Nitrogen application regulates antioxidant capacity and flavonoid metabolism,especially quercetin,in grape seedlings under salt stress

作     者：Congcong Zhang Han Wang Guojie Nai Lei Ma Xu Lu Haokai Yan Meishuang Gong YuanyuanLi Ying Lai Zhihui Pu Li Wei Guiping Chen Ping Sun Baihong Chen Shaoying Ma Sheng Li Congcong Zhang;Han Wang;Guojie Nai;Lei Ma;Xu Lu;Haokai Yan;Meishuang Gong;Yuanyuan Li;Ying Lai;Zhihui Pu;Li Wei;Guiping Chen;Ping Sun;Baihong Chen;Shaoying Ma;Sheng Li

作者机构：College of HorticultureGansu Agricultural UniversityLanzhou 730070China College of Life Science and TechnologyGansu Agricultural UniversityLanzhou 730070China College of AgronomyGansu Agricultural UniversityLanzhou 730070China Laboratory and Practice Base Management CenterGansu Agricultural UniversityLanzhou 730070China State Key Laboratory of Aridland Crop ScienceGansu Agricultural UniversityLanzhou 730070China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第12期

页      面：4074-4092页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the Key Talent Project of Gansu Provincial Party Committee Organization Department Funding,China(2023RCXM23) the Industrial Support of Gansu Provincial Department of Education Funding,China(2021CYZC-55) the Key Research and Development Projects of Gansu Provincial Funding,China(21YF5NA090) 

主　　题：grapevine salt stress nitrogen multi-omics quercetin antioxidant 

摘      要：Salt stress is a typical abiotic stress in plants that causes slow growth,stunting,and reduced yield and fruit *** is necessary to ensure proper crop ***,the effect of fertilization on salt tolerance in grapevine is *** this study,we investigated the effect of nitrogen fertilizer(0.01 and 0.1 mol L^(-1)NH_(4)NO_(3))application on the salt(200 mmol L^(-1)NaCl)tolerance of grapevine based on physiological indices,and transcriptomic and metabolomic *** results revealed that 0.01 mol L^(-1)NH_(4)NO_(3) supplementation significantly reduced the accumulation of superoxide anion(O_(2)^(-)·),enhanced the activities of superoxide dismutase(SOD)and peroxidase(POD),and improved the levels of ascorbic acid(AsA)and glutathione(GSH)in grape leaves compared to salt treatment ***,joint transcriptome and metabolome analyses showed that the differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)were significantly enriched in the flavonoid biosynthesis pathway(ko00941)and the flavone and flavonol biosynthesis pathway(ko00944).In particular,the relative content of quercetin(C00389)was markedly regulated by salt and *** analysis revealed that exogenous foliar application of quercetin improved the SOD and POD activities,increased the AsA and GSH contents,and reduced the H_(2)O_(2) and O_(2)^(-)·***,10 hub DEGs,which had high Pearson correlations(R^(2)0.9)with quercetin,were repressed by *** conclusion,all the results indicated that moderate nitrogen and quercetin application under salt stress enhanced the antioxidant system defense response,thus providing a new perspective for improving salt tolerance in grapes.