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Crowberry(Empetrum nigrum var. japonicum) inhibits cell proliferation and migration by targeting DEK via the Akt signaling pathway in cholangiocarcinoma cells

Crowberry(Empetrum nigrum var. japonicum) inhibits cell prol...

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2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青年科学家论坛

作者： xuebing zhou Xue Wang Ludan Zhang Renbo An Xiangshan Ren YANBIAN University

Background: Cholangiocarcinoma(CCA) is a rare biliary adenocarcinoma related with poor clinical prognosis. Crowberry is used as herbal medicine to control inflammatory diseases and repair cellular antioxidant enzyme activity. Although crowberry has shown significant therapeutic efficacy about the treatment of various tumors and diseases, its anti-cancer results and specific molecular mechanisms in CCA remain poorly *** of the study: The main purpose of this study is to survey the effects of crowberry on the biological behavior of CCA *** and Methods: Chemical profiles of the extract were qualitatively analyzed using highperformance liquid chromatography(HPLC) and HPLC--tandem mass spectrometry. MTT method,colony formation assay and EdU assay were used to measure cell proliferation. The effect of crowberry on CCA cell migration was assessed by wound healing method and migration ***, hochest assays and flow cytometry were used to assessed the cell apoptosis. Western blotting was used to assess the protein expression levels of key factors associated with apoptosis, the Akt signaling pathway, and epithelial-mesenchymal transition. Xenograft model,immunohistochemistry staining and H&E staining were used to assessed the effect of crowberry in ***: Crowberry clearly inhibited the proliferation and migration of CCA cells in a dose-dependent manner and induced apoptosis. Furthermore, crowberry targeted the PI3 K/Akt signaling pathway by obviously altering the expression levels/activation of several key mediators and reducing the expression of the proto-oncogene DEK in vivo and ***: Crowberry inhibits the proliferation and migration of CCA cells by downregulating DEK through the Akt signaling pathway.

关键词： Cholangiocarcinoma Empetrum nigrum var japonicum Proliferation Migration DEK

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Lidocaine hydrochloride loaded isomaltulose microneedles for efficient local anesthesia of the skin

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Chinese Chemical Letters 2024年第4期35卷 298-302页

作者： xuebing Jiang Siyi Wang Li Zhang Xian Jiang Maling Gou State Key Laboratory of Biotherapy West China HospitalSichuan UniversityChengdu 610041China Huahang Microcreate Technology Co. Ltd.Chengdu 610042China Department of Dermatology West China HospitalSichuan UniversityChengdu 610041China

Lidocaine hydrochloride(LIDH) as an anesthetic is widely used in local anesthesia. Dissolving microneedles(MNs) have great application value in the field of skin anesthesia. However, the limited drug-loading of dissolving MNs is an existing challenge that affects clinical use. In this study, we have screened isomaltulose(ISO) as the proper matrix material for the MNs by using molecular dynamics(MD) simulation. Our findings indicate that ISO has good compatibility with LIDH, and the LIDH-loaded ISO MNs(LI-MNs) have high drug-loading capacity. The drug-loading capacity of LI-MNs could reach 80%, and it could effectively puncture the skin. In addition, the preparation method of customized LI-MNs was established based on three-dimensional(3D) printing technology. It was shown that the administration time of LI-MNs could be controlled within 3 min. Also, the LI-MNs were able to provide the local anesthetic efficacy within2 min and sustained for more than 2 h. Significantly, LI-MNs had more efficient drug efficacy compared to the topical creams and the majority of existing LIDH-loaded dissolving MNs. They even provided a longer duration of action than the injections. Overall, the LI-MNs with high drug-loading have a promising application prospect.

关键词： Microneedles Local anesthesia Lidocaine hydrochloride Isomaltulose Transdermal delivery

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Access to Versatile Functionalized Cu(Ⅲ) Complexes Enabled by Direct Transmetalation to Well-Defined Copper(Ⅲ) Fluoride Complex Me_(4)N^(+)[Cu(CF_(3))_(3)F]^(-)

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Chinese Journal of Chemistry 2024年第10期42卷 1107-1113页

作者： Guangyu Wang Huaiyuan Li xuebing Leng Long Lu Qilong Shen Key Laboratory of Fluorine and Nitrogen Chemistry and Advanced Materials Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesChinese Academy of Sciences345 Lingling LuShanghai 200032China

The invention of a well-defined Cu(Ⅲ)fluoride complex Me_(4)N^(+)[Cu(CF_(3))_(3)(F)]^(-)1 enabled to access a versatile of functionalized Cu(Ⅲ)complexes[Me_(4)N]^(+)[Cu(X)(CF_(3))_(3)]^(-)(X=C_(6)F_(5),C_(6)F_(5)C≡C,CN,Cl,N_(3),^(t)BuOO,SCN,OAc,SAr),many of them for the first *** availability of these complexes allowed us to evaluate the trans-influence order of ligand in Cu(Ⅲ)complexes:Bn>CF_(3)^(-)>C_(6)F_(5)^(-)>N_(3)^(-)>py~CH_(3)^(-)~C_(6)F_(5)C≡C>NO_(2)PhO^(-)~^(t)BuOO^(-)~CH_(3)COO^(-)>F^(-).

关键词： Fluorine Copper Cu(Ⅲ) Cu(Ⅲ)fluoride Trifluoromethyl

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Periodontitis exacerbates pulmonary hypertension by promoting IFNγ^(+)T cell infiltration in mice

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International Journal of Oral Science 2024年第2期16卷 359-369页

作者： Xiaoqian Meng Linjuan Du Shuo Xu Lujun zhou Boyan Chen Yulin Li Chumao Chen Huilin Ye Jun Zhang Guocai Tian xuebing Bai Ting Dong Wenzhen Lin Mengjun Sun Kecong zhou Yan Liu Wuchang Zhang Shengzhong Duan Department of Endodontics Shanghai Ninth People’s HospitalCollege of StomatologyShanghai Jiao Tong University School of MedicineShanghaiChina Laboratory of Oral Microbiota and Systemic Diseases Shanghai Ninth People’s HospitalCollege of StomatologyShanghai Jiao Tong University School of MedicineNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of StomatologyShanghaiChina Hongqiao International Institute of Medicine Shanghai Tongren Hospital/Faculty of Basic MedicineKey Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of EducationShanghai Jiao Tong University School of MedicineShanghaiChina Department of Orthodontics Shanghai Ninth People’s HospitalCollege of StomatologyShanghai Jiao Tong University School of MedicineShanghaiChina Department of Periodontology Shanghai Ninth People’s HospitalCollege of StomatologyShanghai Jiao Tong University School of MedicineShanghaiChina Stomatology Hospital School of StomatologyZhejiang University School of MedicineHangzhouChina

Uncovering the risk factors of pulmonary hypertension and its mechanisms is crucial for the prevention and treatment of the *** the current study,we showed that experimental periodontitis,which was established by ligation of molars followed by orally smearing subgingival plaques from patients with periodontitis,exacerbated hypoxia-induced pulmonary hypertension in ***,periodontitis dysregulated the pulmonary microbiota by promoting ectopic colonization and enrichment of oral bacteria in the lungs,contributing to pulmonary infiltration of interferon gamma positive(IFNγ^(+))T cells and aggravating the progression of pulmonary *** addition,we identified Prevotella zoogleoformans as the critical periodontitis-associated bacterium driving the exacerbation of pulmonary hypertension by periodontitis,and the exacerbation was potently ameliorated by both cervical lymph node excision and IFNγneutralizing *** study suggests a proof of concept that the combined prevention and treatment of periodontitis and pulmonary hypertension are necessary.

关键词： hypertension prevention interferon

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Subtropical forest macro-decomposers rapidly transfer litter carbon and nitrogen into soil mineral-associated organic matter

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Forest Ecosystems 2024年第2期11卷 131-139页

作者： Guoxiang Niu Tao Liu Zhen Zhao xuebing Zhang Huiling Guan Xiaoxiang He Xiankai Lu National Engineering Laboratory for Applied Technology of Forestry and Ecology in South China Central South University of Forestry and TechnologyChangsha410004China Lushan Botanical Garden Chinese Academy of SciencesJiujiang332900China Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems South China Botanical GardenChinese Academy of SciencesGuangzhou510650China Section of Microbial Ecology Department of BiologyLund UniversityLund22362Sweden Lutou National Station for Scientific Observation and Research of Forest Ecosystem in Hunan Province Yueyang414000China

Background:Forest soils in tropical and subtropical areas store a significant amount of *** framework to assess soil organic matter(SOM)dynamics under evolving global conditions suggest that dividing bulk SOM into particulate and mineral-associated organic matter(POM ***)is a promising method for identifying how SOM contributes to reducing global *** macrofauna,earthworms,and millipedes have been found to play an important role in facilitating SOM ***,how these two co-existing macrofaunae impac the litter decomposition process and directly impact the formation of POM and MAOM remains ***:Here,we set up a microcosm experiment,which consisted of 20 microcosms with four treatments earthworm and litter addition(E),millipedes and litter addition(M),earthworm,millipedes,and litter addition(E+M),and control(only litter addition)in five *** soil and litter were sterilized prior to beginning the incubation experiment to remove any existing *** incubating the samples for 42 days,the litte properties(mass,C,and N contents),soil physicochemical properties,as well as the C and N contents,and POM and MAOM^(13)C abundance in the 0–5 and 5–10 cm soil layers were ***,the relative influences o soil physicochemical and microbial properties on the distribution of C and N in the soil fractions were analyzed Results:The litter mass,C,and N associated with all four treatments significantly decreased after incubation especially under treatment E+M(litter mass:-58.8%,litter C:-57.0%,litter N:-75.1%,respectively),while earthworm biomass significantly decreased under treatment *** or millipede addition alone showed no significant effects on the organic carbon(OC)and total nitrogen(TN)content in the POM fraction,but join addition of both significantly increased OC and TN regardless of soil ***,all three macrofauna treatments increased the OC and TN content and decreased the^(13)C abundance

关键词： Tropical and subtropical forest Soil organic matter fractions Earthworm Millipedes Litter decomposition

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X射线衍射分析聚乙烯吡咯烷酮对水合物分解过程的影响

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化工学报 2021年第2期72卷 1125-1131页

作者：唐翠萍周雪冰梁德青中国科学院广州能源研究所广东广州510640 中国科学院天然气水合物重点实验室广东广州510640 广东省新能源和可再生能源研究开发与应用重点实验室广东广州510640 中国科学院广州天然气水合物研究中心广东广州510640 天然气水合物国家重点实验室北京100028

深水油气资源的勘探开发以及开采过程中的环保要求,使得天然气水合物动力学抑制剂使用不可避免,含动力学抑制剂的分解研究对水合物生成后的解堵具有重要的指导意义。本文在高压反应釜内采用甲烷和丙烷混合气,合成天然气水合物,并用X射... 详细信息

深水油气资源的勘探开发以及开采过程中的环保要求,使得天然气水合物动力学抑制剂使用不可避免,含动力学抑制剂的分解研究对水合物生成后的解堵具有重要的指导意义。本文在高压反应釜内采用甲烷和丙烷混合气,合成天然气水合物,并用X射线粉晶衍射仪分析了含动力学抑制剂聚乙烯吡咯烷酮的水合物分解过程。结果显示甲烷和丙烷气体会形成SⅡ型水合物,但伴随有SⅠ型甲烷水合物的生成;添加动力学抑制剂后,水合物的分解速率变慢,在-60℃,添加0.5%的聚乙烯吡咯烷酮后,分解起始的20 min内,无抑制剂体系水合物分解可达69%,而在含抑制剂体系分解约18%;SⅡ型甲烷丙烷混合气水合物分解过程中晶胞各晶面分解速率相同,没有偏好性,水合物笼作为一个整体分解,添加抑制剂不改变这种分解方式,仍以整体分解。

关键词：天然气水合物动力学抑制剂分解 X射线衍射晶体结构

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Modification of plasma protein for bioprinting via photopolymerization

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Chinese Chemical Letters 2024年第8期35卷 486-490页

作者： Wenbi Wu Yinchu Dong Haofan Liu xuebing Jiang Li Li Yi Zhang Maling Gou Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China Chengdu OrganoidMed Medical Laboratory West China Health ValleyChengdu 610043China

Bioprinting is emerging as an advanced tool in tissue ***,there is still a lack of bioinks able to form hydrogels with desirable bioactivities that support positive cell *** this study,modified plasma proteins capable of forming hydrogels with multiple biological functions are developed as bioinks for digital light processing(DLP)*** Plasma-MA(BM)was synthesized via a one-pot method through the reaction between the fresh frozen plasma and methacrylic *** methacry-lated levels were observed to influence the physical properties of BM hydrogels including mechanical properties,swelling,and *** photo-crosslinked BM hydrogels can sustainedly release vascu-lar endothelial growth factor(VEGF)and exhibit positive biological effects on cell adhesion and prolifer-ation,and cell functionality such as tube formation of human umbilical vein endothelial cells(HUVECs),and neurite elongation of rat pheochromocytoma cells(PC12).Meanwhile,BM hydrogels can also induce cell infiltration,modulate immune response,and promote angiogenesis in ***,the plasma bioinks can be used to fabricate customized scaffolds with complex structures through a DLP printing *** findings implicate that the modified plasma with growth factor release is a promising candidate for bioprinting in autologous and personalized tissue engineering.

关键词： Bioprinting Bioink Photopolymerization Plasma Growth factor

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A multi-feature-based intelligent redundancy elimination scheme for cloud-assisted health systems

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CAAI Transactions on Intelligence Technology 2024年第2期9卷 491-510页

作者： Ling Xiao Beiji Zou Xiaoyan Kui Chengzhang Zhu Wensheng Zhang xuebing Yang Bob Zhang School of Computer Science and Engineering Central South UniversityChangshaChina Hunan Engineering Research Center of Machine Vision and Intelligent Medicine Central South UniversityChangshaChina The College of Literature and Journalism Central South UniversityChangshaChina Institute of Automation Chinese Academy of SciencesBeijingChina Department of Computer and Information Science University of MacaoMacaoChina

Redundancy elimination techniques are extensively investigated to reduce storage overheads for cloud-assisted health *** eliminates the redundancy of duplicate blocks by storing one physical instance referenced by multiple *** compression is usually regarded as a complementary technique to deduplication to further remove the redundancy of similar blocks,but our observations indicate that this is disobedient when data have sparse duplicate *** addition,there are many overlapped deltas in the resemblance detection process of post-deduplication delta compression,which hinders the efficiency of delta compression and the index phase of resemblance detection inquires abundant non-similar blocks,resulting in inefficient system ***,a multi-feature-based redundancy elimination scheme,called MFRE,is proposed to solve these *** similarity feature and temporal locality feature are excavated to assist redundancy elimination where the similarity feature well expresses the duplicate ***,similarity-based dynamic post-deduplication delta compression and temporal locality-based dynamic delta compression discover more similar base blocks to minimise overlapped deltas and improve compression ***,the clustering method based on block-relationship and the feature index strategy based on bloom filters reduce IO overheads and improve system *** demonstrate that the proposed method,compared to the state-of-the-art method,improves the compression ratio and system throughput by 9.68%and 50%,respectively.

关键词： big data cloud computing compression data compression medical applications performance evaluation

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Study on the effects of thrombin on AQP4 mRNA and AQP4 protein expression in rat primary astrocytes

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Journal of Nanjing Medical University 2006年第4期20卷 220-224页

作者： Jinghua zhou xuebing Cao Shenggang Sun Department of Neurology Union Hospital Tongii Medical College Huazhong University of Science and Technology Wuhan 430022 China

Objective： To study the biologic effects of various concentrations of thrombin on aquaporin 4 （AQP4） expression in rat primary cultured astrocytes, and to explore the regulation mechanism of transmembrane water transportation in astrocytes after intracerebral hemorrhage （ICH）. Methods： Primary cultured astrocytes were incubated in culture mediums containing various concentrations of thrombin for 24 h and harvested. AQP4 mRNA and AQP4 protein expression were determined by reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemical technique. Cell apoptosis was detected by TdT- mediated dUTP nick end labeling （TUNEL） technique. Cell morphology was observed by phase contrast microscope, and cell viability was assayed by MTT. Results： AQP4 mRNA and AQP4 protein showed a low expression in normal astrocytes. The expression of AQP4 mRNA and AQP4 protein significantly increased in the astrocytes treated with 100 U/ml or 200 U/ml thrombin （P 〈 0.01 ）,and these astrocytes swelled. The number of TUNEL positive cells significantly increased. On the other hand, AQP4 mRNA and AQP4 protein expression were downegulated in the astrocytes treated with 0.5 U/ml or 1 U/ml thrombin （P 〈 0.05）, and the cell morphology did not change. Few TUNEL positive cells were observed. Conclusion： AQP4 overxpression induced by high concentrations of thrombin causes an increased permeability of water in astrocytic membrane. On the contrary, the decreased AQP4 expression prevents the astrocytes from swelling and apoptosis.

关键词： thrombin astrocytes aquaporin-4 apoptosis

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Variation in Bombyx mori immune response against fungal pathogen Beauveria bassiana with variability in cell wallβ-1,3-glucan

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Insect Science 2024年第1期31卷 211-224页

作者： Yulong Wang Zhen Liu xuebing Yin Shihong Liu Kai Wang Rongjie Wan Haoran Chen Xinyang Li Bo Huang Anhui Provincial Key Laboratory of Microbial Pest Control Anhui Agricultural UniversityHefeiChina

Entomopathogenic fungi are protected by a cell wall with dynamic structure for adapting to various environmental conditions.β-1,3-Glucan recognition proteins activate the innate immune system of insects by recognizing surface molecules of ***,the associations between pathogenicity and the different components of entomopathogenic fungal cell walls remain *** Beauveria bassiana strains were selected that have significantly differing virulence against Bombyx *** molecular mechanisms underlying the immune response in *** were investigated using RNA sequencing,which revealed differences in the immune response to different *** strains at 12 h *** assays revealed thatβ-1,3-glucan content had an opposite trend to that of fungal virulence.β-1,3-Glucan injection upregulated BmβGRP4 expression and significantly reduced the virulence of the high-virulence strain but not that of the medium-virulence or low-virulence ***βGRP4 silencing in *** with RNA interference resulted in the opposite virulence pattern,indicating that the virulence of *** was affected by the cell walls’content ofβ-1,3-glucan,which could be recognized by Bmβ***,interference with the gene CnA(calcineurin catalytic A subunit)involved inβ-1,3-glucan synthesis eliminated differences in virulence between *** *** results indicate that strains of a single species of pathogenic fungi that have differing cell wall components are recognized differently by the innate immune system of ***.

关键词： ombyx mori BmβGRP4 innate immunity insect fungal pathogen virulence

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