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Science China Chemistry 2020年第2期63卷 182-186页

作者： Kai Sheng Li-Ming Fan xue-fei tian Rakesh Kumar Gupta Linna Gao Chen-Ho Tung Di Sun Key Laboratory of Colloid and Interface Chemistry Ministry of EducationSchool of Chemistry and Chemical EngineeringState Key Laboratory of Crystal MaterialsShandong UniversityJinan 250100China School of Aeronautics Shandong Jiaotong UniversityJinan 250037China Department of Chemistry College of ScienceNorth University of ChinaTaiyuan 030051China College of Chemical and Environmental Engineering Shandong University of Science and TechnologyQingdao 266590China

Two novel Sn(Ⅱ) supramolecular isomeric frameworks,with the identical formula of {(NH2Me2)2[Sn(BDC)(SO4)]}n,Sn-CP-1-α(1) and Sn-CP-1-β(2)(H2BDC=terephthalic acid) were synthesized under solvothermal condition and fully characterized by single crystal X-ray diffraction(SCXRD),Fourier transform infrared spectroscopy(FTIR),ultraviolet-visible spectroscopy(UVVis),elemental analyses,and thermogravimetric analysis(TGA).Interestingly,the structures of 1 and 2 are governed by the temperature of the reaction,suggesting a temperature-induced supramolecular *** supramolecular isomers are primarily caused by the different bridging alignments of SO42–.Compounds 1 and 2 display 2 D layer and 3 D framework with different topologies,non-interpenetrated 44-sql and two-fold interpenetrated 4-connected dia topology,*** to Lewis acid properties of coordinatively unsaturated Sn(Ⅱ) sites in CPs,they have been utilized as heterogeneous catalyst for the cyanosilylation of aldehydes with an excellent conversion yield over 99% under solvent-free conditions.

关键词： supramolecular isomerism Sn(Ⅱ)coordination polymers catalysis cyanosilylation

Synergistic anti-liver cancer effects of curcumin and total ginsenosides

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World Journal of Gastrointestinal Oncology 2020年第10期12卷 1091-1103页

作者： Zhe Deng Xiao-Yan Xu Fenny Yunita Qing Zhou Yong-Rong Wu Yu-Xing Hu Zhi-Qi Wang xue-fei tian Department of Internal Medicine College of Integrated Chinese and Western MedicineHunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Hunan Key Laboratory of Translational Research in Formulas and Zheng of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina The First Affiliated Hospital Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina School of Basic Chinese Medical Sciences Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina College of Pharmaceutical Sciences Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina

BACKGROUND Liver cancer is the sixth most frequently occurring cancer in the world and the fourth most common cause of cancer *** pathogenesis of liver cancer is closely associated with inflammation and immune response in the tumor *** therapeutic agents for liver cancer,which can control inflammation and restore cellular immunity,are ***(Cur)is a natural anti-inflammatory drug,and total ginsenosides(TG)are a commonly used immunoregulatory *** note,both Cur and TG have been shown to exert anti-liver cancer *** To determine the synergistic immunomodulatory and anti-inflammatory effects of Cur combined with TG in a mouse model of subcutaneous liver *** A subcutaneous liver cancer model was established in BALB/c mice by a subcutaneous injection of hepatoma cell *** were treated with Cur(200 mg/kg per day),TG(104 mg/kg per day or 520 mg/kg per day),the combination of Cur(200 mg/kg per day)and TG(104 mg/kg per day or 520 mg/kg per day),or 5-fluorouracil combined with cisplatin as a positive control for 21 *** volume was measured and the protein expression of programmed cell death 1 and programmed cell death 1 ligand 1(PD-L1),inflammatory indicators Toll like receptor 4(TLR4)and nuclear factor-κB(NF-κB),and vascular growth-related factors nitric oxide synthases(iNOS)and matrix metalloproteinase 9 were analyzed by Western blot ***4+CD25+Foxp3+regulatory T cells(Tregs)were counted by flow *** The combination therapy of Cur and TG significantly inhibited the growth of liver cancer,as compared to vehicle-treated animals,and TG showed dose *** combined with TG-520 markedly decreased the protein expression of PD-L1(P<0.0001),while CD4+CD25+Foxp3+Tregs regulated by the PD-L1 signaling pathway exhibited a positive correlation with *** combined with TG-520 also inhibited the cascade action mediated by NF-κB(P<0.0001),thus inhibiting the TLR4/NF-κB signalling pathway(

关键词： Total ginsenosides Curcumin Liver cancer Immune Inflammation Programmed cell death 1 ligand 1

Mediating role of social support in dysphoria,despondency,and quality of life in patients undergoing maintenance hemodialysis

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World Journal of Psychiatry 2024年第3期14卷 409-420页

作者： Xiang Zhou Hong Jiang Yi-Peng Zhou Xiao-Yu Wang Hai-Yan Ren xue-fei tian Qing-Qing Zhang Department of Nephrology People’s Hospital of Xinjiang Uygur Autonomous RegionXinjiang Clinical Research Center for Kidney DiseaseUrumqi 832000Xinjiang Uygur Autonomous RegionChina Department of Endocrinology and Metabolism People’s Hospital of Xinjiang Uygur Autonomous RegionUrumqi 832000Xinjiang Uygur Autonomous RegionChina Section of Nephrology Department of Internal MedicineYale University School of MedicineNew HavenCT 06510United States

BACKGROUND Dysphoria and despondency are prevalent psychological issues in patients undergoing Maintenance Hemodialysis(MHD)that significantly affect their quality of life(QOL).High levels of social support can significantly improve the physical and mental well-being of patients undergoing ***,there is limited research on how social support mediates the relationship between dysphoria,despondency,and overall QOL in patients undergoing *** is imperative to investigate this mediating effect to mitigate dysphoria and despondency in patients undergoing MHD,ultimately enhancing their overall *** To investigate the mediating role of social support in relationships between dysphoria,despondency,and QOL among patients undergoing *** Participants comprised 289 patients undergoing MHD,who were selected using a random sampling *** Social Support Rating Scale,Self-Rating Anxiety Scale,Self-Rating Depression Scale,and QOL Scale were *** analysis was performed to examine the associations between social support,dysphoria,despondency,and QOL in patients undergoing *** assess the mediating impact of social support on dysphoria,despondency,and QOL in patients undergoing MHD,a bootstrap method was *** Significant correlations among social support,dysphoria,despondency,and quality in patients undergoing MHD were observed(all P<0.01).Dysphoria and despondency negatively correlated with social support and QOL(P<0.01).Dysphoria and despondency had negative predictive impacts on the QOL of patients undergoing MHD(P<0.05).The direct effect of dysphoria on QOL was statistically significant(P<0.05).Social support mediated the relationship between dysphoria and QOL,and this mediating effect was significant(P<0.05).Similarly,the direct effect of despondency on QOL was significant(P<0.05).Moreover,social support played a mediating role between despondency and QOL,with a significant mediating effect(P<0.05).CONCLUSION These find

关键词： Maintenance hemodialysis Social support Dysphoria Despondency Quality of life Mediating effect

Antihepatoma peptide,scolopentide,derived from the centipede scolopendra subspinipes mutilans

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World Journal of Gastroenterology 2023年第12期29卷 1875-1898页

作者： Yu-Xing Hu Zhuo Liu Zhen Zhang Zhe Deng Zhen Huang Ting Feng Qing-Hong Zhou Si Mei Chun Yi Qing Zhou Pu-Hua Zeng Gang Pei Sha tian xue-fei tian College of Integrated Chinese and Western Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Hunan Key Laboratory of Translational Research in Formulas and Zheng of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Scientific Research Hunan Academy of Traditional Chinese Medicine Affiliated HospitalChangsha 410208Hunan ProvinceChina Hunan Province University Key Laboratory of Oncology of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Pediatric Shenzhen Hospital of Beijing University of Chinese MedicineShenzhen 518000Guangdong ProvinceChina Department of Physiology Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Pathology Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Andrology First Hospital of Hunan University of Chinese MedicineChangsha 410007Hunan ProvinceChina Department of Oncology Hunan Academy of Traditional Chinese Medicine Affiliated HospitalChangsha 410208Hunan ProvinceChina College of Pharmacy Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Dr Neher’s Biophysics Laboratory for Innovative Drug Discovery Macao University of Science and TechnologyMacao 999078China

BACKGROUND Centipedes have been used to treat tumors for hundreds of years in ***,current studies focus on antimicrobial and anticoagulation agents rather than *** molecular identities of antihepatoma bioactive components in centipedes have not yet been extensively *** is a challenge to isolate and characterize the effective components of centipedes due to limited peptide purification technologies for animal-derived *** To purify,characterize,and synthesize the bioactive components with the strongest antihepatoma activity from centipedes and determine the antihepatoma *** An antihepatoma peptide(scolopentide)was isolated and identified from the centipede scolopendra subspinipes mutilans using a combination of enzymatic hydrolysis,a Sephadex G-25 column,and two steps of high-performance liquid chromatography(HPLC).Additionally,the CCK8 assay was used to select the extracted fraction with the strongest antihepatoma *** molecular weight of the extracted scolopentide was characterized by quadrupole time of flight mass spectrometry(QTOF MS),and the sequence was matched by using the Mascot search *** on the sequence and molecular weight,scolopentide was synthesized using solid-phase peptide synthesis *** synthetic scolopentide was confirmed by MS and *** antineoplastic effect of extracted scolopentide was confirmed by CCK8 assay and morphological changes again in *** antihepatoma effect of synthetic scolopentide was assessed by the CCK8 assay and Hoechst staining in vitro and tumor volume and tumor weight in *** the tumor xenograft experiments,qualified model mice(male 5-week-old BALB/c nude mice)were randomly divided into 2 groups(n=6):The scolopentide group(0.15 mL/d,via intraperitoneal injection of synthetic scolopentide,500 mg/kg/d)and the vehicle group(0.15 mL/d,via intraperitoneal injection of normal saline).The mice were euthanized by cervical dislocation after 14 d of con

关键词： Scolopendra Centipede Antihepatom peptide Hepatocellular carcinoma Death receptor 4 Death receptor 5

Frankincense myrrh attenuates hepatocellular carcinoma by regulating tumor blood vessel development through multiple epidermal growth factor receptor-mediated signaling pathways

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World Journal of Gastrointestinal Oncology 2022年第2期14卷 450-477页

作者： Piao Zheng Zhen Huang Dong-Chang Tong Qing Zhou Sha tian Bo-Wei Chen Di-Min Ning Yin-Mei Guo Wen-Hao Zhu Yan Long Wei Xiao Zhe Deng Yi-Chen Lei xue-fei tian College of Integrated Traditional Chinese and Western Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Integrated Traditional Chinese and Western Medicine The Second Xiangya HospitalCentral South UniversityChangsha 410011Hunan ProvinceChina The First Affiliated Hospital Hunan University of Chinese MedicineChangsha 410021Hunan ProvinceChina Hunan Key Laboratory of Translational Research in Formulas and Zheng of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Institute of Integrative Medicine Department of Integrated Traditional Chinese and Western MedicineXiangya HospitalCentral South UniversityChangsha 410008Hunan ProvinceChina

BACKGROUND In traditional Chinese medicine(TCM),frankincense and myrrh are the main components of the antitumor drug Xihuang *** compounds show anticancer activity in other biological ***,whether frankincense and/or myrrh can inhibit the occurrence of hepatocellular carcinoma(HCC)is unknown,and the potential molecular mechanism(s)has not yet been *** To predict and determine latent anti-HCC therapeutic targets and molecular mechanisms of frankincense and myrrh in *** In the present study,which was based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(http://***/***),Universal Protein database(http://***),GeneCards:The Human Gene Database(http://***/)and Comparative Toxicogenomics Database(http://***/),the efficacy of and mechanism by which frankincense and myrrh act as anti-HCC compounds were *** core prediction targets were screened by molecular *** vivo,SMMC-7721 human liver cancer cells were transplanted as xenografts into nude mice to establish a subcutaneous tumor model,and two doses of frankincense plus myrrh or one dose of an EGFR inhibitor was administered to these mice continuously for 14 *** tumors were collected and evaluated:the tumor volume and growth rate were gauged to evaluate tumor growth;hematoxylineosin staining was performed to estimate histopathological changes;immunofluorescence(IF)was performed to detect the expression of CD31,α-SMA and collagen IV;transmission electron microscopy(TEM)was conducted to observe the morphological structure of vascular cells;enzyme-linked immunosorbent assay(ELISA)was performed to measure the levels of secreted HIF-1αand TNF-α;reverse transcription-polymerase chain reaction(RT-qPCR)was performed to measure the mRNA expression of HIF-1α,TNF-α,VEGF and MMP-9;and Western blot(WB)was performed to determine the levels of proteins expressed in the EGFR-mediated PI3K/Akt and

关键词： Hepatocellular carcinoma Frankincense Myrrh Network pharmacology Tumor blood vessels Multiple signaling pathways

Calculus bovis inhibits M2 tumor-associated macrophage polarization via Wnt/β-catenin pathway modulation to suppress liver cancer

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World Journal of Gastroenterology 2024年第29期30卷 3511-3533页

作者： Zhen Huang Fan-Ying Meng Lin-Zhu Lu Qian-Qian Guo Chang-Jun Lv Nian-Hua Tan Zhe Deng Jun-Yi Chen Zi-Shu Zhang Bo Zou Hong-Ping Long Qing Zhou Sha tian Si Mei xue-fei tian College of Integrated Chinese and Western Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Hunan Key Laboratory of Translational Research in Formulas and Zheng of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Key Laboratory of Traditional Chinese Medicine for Mechanism of Tumor Prevention and Treatment Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina The First Clinical College of Traditional Chinese Medicine Hunan University of Traditional Chinese MedicineChangsha 410007Hunan ProvinceChina Department of Hepatology Hunan University of Chinese MedicineChangsha 410007Hunan ProvinceChina The First Hospital of Hunan University of Chinese Medicine Hunan University of Chinese MedicineChangsha 410007Hunan ProvinceChina Faculty of Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina

BACKGROUND Calculus bovis(CB),used in traditional Chinese medicine,exhibits anti-tumor effects in various cancer *** also constitutes an integral component of a compound formulation known as Pien Tze Huang,which is indicated for the treatment of liver ***,its impact on the liver cancer tumor microenvironment,particularly on tumor-associated macrophages(TAMs),is not well *** To elucidate the anti-liver cancer effect of CB by inhibiting M2-TAM polarization via Wnt/β-catenin pathway *** This study identified the active components of CB using UPLC-Q-TOF-MS,evaluated its anti-neoplastic effects in a nude mouse model,and elucidated the underlying mechanisms via network pharmacology,transcriptomics,and molecular *** vitro assays were used to investigate the effects of CB-containing serum on HepG2 cells and M2-TAMs,and Wnt pathway modulation was validated by real-time reverse transcriptase-polymerase chain reaction and Western blot *** This study identified 22 active components in CB,11 of which were detected in the *** investigations have demonstrated the ability of CB to effectively inhibit liver tumor *** integrated approach employing network pharmacology,transcriptomics,and molecular docking implicated the Wnt signaling pathway as a target of the antineoplastic activity of CB by suppressing M2-TAM *** vitro and in vivo experiments further confirmed that CB significantly hinders M2-TAM polarization and suppresses Wnt/β-catenin pathway *** inhibitory effect of CB on M2-TAMs was reversed when treated with the Wnt agonist SKL2001,confirming its pathway *** This study demonstrated that CB mediates inhibition of M2-TAM polarization through the Wnt/β-catenin pathway,contributing to the suppression of liver cancer growth.

关键词： Calculus bovis M2 tumor-associated macrophage polarization Liver cancer Wnt/β-catenin pathway Tumor microenvironment

Shuyu pills inhibit immune escape and enhance chemosensitization in hepatocellular carcinoma

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World Journal of Gastrointestinal Oncology 2021年第11期13卷 1725-1740页

作者： Zhe Deng Yong-Jie Teng Qing Zhou Zhao-Guang Ouyang Yu-Xing Hu Hong-Ping Long Mei-Jie Hu Si Mei Feng-Xia Lin Xin-Jun Dai Bo-Yu Zhang Ting Feng xue-fei tian College of Integrated Chinese and Western Medicine Hunan Key Laboratory of Translational Research in Formulas and Zheng of Traditional Chinese MedicineHunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina The First Hospital of Hunan University of Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Preventive Dentistry Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative MedicineGuangzhou Medical UniversityGuangzhou 510132Guangdong ProvinceChina Experiment Center of Medical Innovation The First Hospital of Hunan University of Chinese MedicineHunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Physiology Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Cardiology Shenzhen Bao'an Traditional Chinese Medicine Hospital GroupThe Affiliated Hospital of Guangzhou University of Chinese MedicineShenzhen 518133Guangdong ProvinceChina College of Acupuncture and Massage Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina

BACKGROUND Hepatocellular carcinoma(HCC)is characterized by dysregulation of the immune microenvironment and the development of ***,expression of the programmed cell death protein 1(PD-1)/programmed cell death 1 ligand 1(PD-L1)axis,an immune checkpoint,may lead to tumour immune escape,resulting in disease *** latest research shows that tumour immune escape may be caused by the upregulation of PD-L1 mediated by hypoxia-inducible factor-1 alpha(HIF-1α),and simultaneous inhibition of HIF-1αand PD-L1 has the potential to enhance the host’s antitumour ***,inhibition of the PD-1/PD-L1 axis may mitigate tumour *** pills(SYPs)contain immunity-enhancing and antitumour components,making them a potential HCC *** To investigate the efficacy of SYPs for HCC treatment via simultaneous HIF-1α and PD-L1 inhibition and the mechanism *** A subcutaneous xenograft tumour model was first established in BALB/c nude mice by the subcutaneous injection of 1×107 SMMC-7721 *** mice(male,5 weeks old;n=24)were then randomly divided into the following four groups(n=6):Control(0.9%normal saline),SYP(200 mg/kg),SYP+cisplatin(DDP)(200 mg/kg+5 mg/kg DDP weekly via intraperitoneal injection),and DDP(5 mg/kg cisplatin weekly via intraperitoneal injection).The dose of saline or SYPs for the indicated mouse groups was 0.2 mL/d via intragastric *** tumour volumes and body weights of the mice were measured every 2 *** mice were euthanized by cervical dislocation after 14 d of continuous treatment,and the xenograft tissues were excised and *** blot assays were used to measure the protein expression of HIF-1α,PD1,PD-L1,CD4+T cells,and CD8+T cells in HCC tumours from *** reverse transcription polymerase chain reaction was used for real-time quantitative detection of PD-1,PD-L1,and HIF-1α mRNA *** immunofluorescence assay was conducted to examine the expressio

关键词： Shuyu pills Hepatocellular carcinoma Tumour microenvironment Immune escape Chemoresistance

Xihuang pills induce apoptosis in hepatocellular carcinoma by suppressing phosphoinositide 3-kinase/protein kinase- B/mechanistic target of rapamycin pathway

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World Journal of Gastrointestinal Oncology 2022年第4期14卷 872-886页

作者： Yong-Jie Teng Zhe Deng Zhao-Guang Ouyang Qing Zhou Si Mei Xing-Xing Fan Yong-Rong Wu Hong-Ping Long Le-Yao Fang Dong-Liang Yin Bo-Yu Zhang Yin-Mei Guo Wen-Hao Zhu Zhen Huang Piao Zheng Di-Min Ning xue-fei tian The First Hospital of Hunan University of Chinese Medicine Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina College of Integrated Chinese and Western Medicine Hunan Key Laboratory of Translational Research in Formulas and Zheng of Traditional Chinese MedicineHunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Preventive Dentistry Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative MedicineGuangzhou Medical UniversityGuangzhou 510132Guangdong ProvinceChina Department of Physiology Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina State Key Laboratory of Quality Research in Chinese Medicine Macao Institute For Applied Research in Medicine and HealthMacao University of Science and TechnologyMacaoChina School of Pharmacy Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina College of Acupuncture and Massage Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina

BACKGROUND The phosphoinositide 3-kinase/protein kinase-B/mechanistic target of rapamycin(PI3K/Akt/mTOR) signalling pathway is crucial for cell survival, differentiation, apoptosis and metabolism. Xihuang pills(XHP) are a traditional Chinese preparation with antitumour properties. They inhibit the growth of breast cancer, glioma, and other tumours by regulating the PI3K/Akt/mTOR signalling pathway. However, the effects and mechanisms of action of XHP in hepatocellular carcinoma(HCC) remain unclear. Regulation of the PI3K/Akt/mTOR signalling pathway effectively inhibits the progression of HCC. However, no study has focused on the XHPassociated PI3K/Akt/mTOR signalling pathway. Therefore, we hypothesized that XHP might play a role in inhibiting HCC through the PI3K/Akt/mTOR signalling *** To confirm the effect of XHP on HCC and the possible mechanisms *** The chemical constituents and active components of XHP were analysed using ultra-performance liquid chromatography-quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS). Cellbased experiments and in vivo xenograft tumour experiments were utilized to evaluate the effect of XHP on HCC tumorigenesis. First, SMMC-7721 cells were incubated with different concentrations of XHP(0, 0.3125, 0.625, 1.25, and 2.5 mg/mL) for 12 h, 24 h and 48 h. Cell viability was assessed using the CCK-8 assay, followed by an assessment of cell migration using a wound healing ***, the effect of XHP on the apoptosis of SMMC-7721 cells was evaluated. SMMC-7721 cells were stained with fluorescein isothiocyanate and annexin V/propidium iodide. The number of apoptotic cells and cell cycle distribution were measured using flow cytometry. The cleaved protein and mRNA expression levels of caspase-3 and caspase-9 were detected using Western blotting and quantitative reverse-transcription polymerase chain reaction(RT-qPCR), ***, Western blotting and RT–qPCR were performed to confirm the effects of XHP on

关键词： Hepatocellular carcinoma Xihuang pills Apoptosis Antitumour Phosphoinositide 3-kinase/protein kinase-B/mechanistic target of rapamycin pathway

Advances in the Development of Gradient Scaffolds Made of Nano‑Micromaterials for Musculoskeletal Tissue Regeneration

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Nano-Micro Letters 2025年第3期17卷 455-500页

作者： Lei Fang Xiaoqi Lin Ruian Xu Lu Liu Yu Zhang Feng tian Jiao Jiao Li Jiajia xue Beijing Laboratory of Biomedical Materials Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China School of Biomedical Engineering Faculty of Engineering and ITUniversity of Technology SydneySydneyNSW 2007Australia

The intricate hierarchical structure of musculoskeletal tissues,including bone and interface tissues,necessitates the use of complex scaffold designs and material structures to serve as tissue-engineered *** has led to growing interest in the development of gradient bone scaffolds with hierarchical structures mimicking the extracellular matrix of native tissues to achieve improved therapeutic *** on the anatomical characteristics of bone and interfacial tissues,this review provides a summary of current strategies used to design and fabricate biomimetic gradient scaffolds for repairing musculoskeletal tissues,specifically focusing on methods used to construct compositional and structural gradients within the *** latest applications of gradient scaffolds for the regeneration of bone,osteochondral,and tendon-to-bone interfaces are ***,the current progress of testing gradient scaffolds in physiologically relevant animal models of skeletal repair is discussed,as well as the challenges and prospects of moving these scaffolds into clinical application for treating musculoskeletal injuries.

关键词： Gradient scaffolds Musculoskeletal tissues Advanced manufacturing Biomaterials Tissue regeneration

感染幽门螺杆菌小鼠肝组织Cyclin D1和PCNA的表达

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世界华人消化杂志 2006年第14期14卷 1341-1345页

作者：田雪飞范学工黄燕张艳朱才中南大学湘雅医院传染科湖南省长沙市410008

目的:探讨经口接种制作的幽门螺杆菌(H pylori)小鼠感染模型H Pylori对肝组织Cyclin D1和PCNA表达的影响．方法:C57BL／6小鼠30只经口感染接种H pylori悉尼株(SS1),置层流柜中饲养8 mo．用巢式PCR检测肝组织H pylori 16S rRNA,并提取阳... 详细信息

目的:探讨经口接种制作的幽门螺杆菌(H pylori)小鼠感染模型H Pylori对肝组织Cyclin D1和PCNA表达的影响．方法:C57BL／6小鼠30只经口感染接种H pylori悉尼株(SS1),置层流柜中饲养8 mo．用巢式PCR检测肝组织H pylori 16S rRNA,并提取阳性样本的mRNA与蛋白,用RT-PCR、 Western blot等方法检测小鼠肝组织癌基因 Cyclin D1和PCNA mRNA及蛋白表达变化．结果:巢式PCR检测15只小鼠肝组织中H pylori DNA,有6只16S rRNA基因为阳性,测序结果经序列分析后显示与胃黏膜分离培养细菌、接种细菌同源性100％．肝组织内有 H pylori定植的6只感染小鼠肝组织Cyclin D1 和PCNA mRNA表达升高(0．78±0．13 vs 0．66 ±0．03,P<0．05:0．86±0．17 vs 0．56±0．24, P<0．01)．Western blot结果显示,Cyclin D1蛋白表达有增高趋势,但与对照组比较无显著性差异;PCNA蛋白表达增高,与对照组比较有显著性差异(1．16±0．40 vs 0．64±0．11,P<0．05)．结论:经口接种H Pylori可达小鼠肝脏,使 Cyclin D1和PCNA表达增强．

关键词：幽门螺杆菌肝细胞癌 PCNA Cyclin D1 小鼠