Antihepatoma peptide,scolopentide,derived from the centipede scolopendra subspinipes mutilans
作者机构：College of Integrated Chinese and Western MedicineHunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Hunan Key Laboratory of Translational Research in Formulas and Zheng of Traditional Chinese MedicineHunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of Scientific ResearchHunan Academy of Traditional Chinese Medicine Affiliated HospitalChangsha 410208Hunan ProvinceChina Hunan Province University Key Laboratory of Oncology of Traditional Chinese MedicineHunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of PediatricShenzhen Hospital of Beijing University of Chinese MedicineShenzhen 518000Guangdong ProvinceChina Department of PhysiologyHunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of PathologyHunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Department of AndrologyFirst Hospital of Hunan University of Chinese MedicineChangsha 410007Hunan ProvinceChina Department of OncologyHunan Academy of Traditional Chinese Medicine Affiliated HospitalChangsha 410208Hunan ProvinceChina College of PharmacyHunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Dr Neher’s Biophysics Laboratory for Innovative Drug DiscoveryMacao University of Science and TechnologyMacao 999078China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2023年第29卷第12期
页 面：1875-1898页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：Supported by the National Natural Science Foundation of China,No.U20A20408 and No.82074450 Natural Science Foundation of Hunan Province,No.2020JJ4066 and No.2021JJ40405 Key scientific research project of Hunan Education Department,No.21A0243 Key project of academician workstation guidance project,No.21YSZQ007
主 题：Scolopendra Centipede Antihepatom peptide Hepatocellular carcinoma Death receptor 4 Death receptor 5
摘 要：BACKGROUND Centipedes have been used to treat tumors for hundreds of years in ***,current studies focus on antimicrobial and anticoagulation agents rather than *** molecular identities of antihepatoma bioactive components in centipedes have not yet been extensively *** is a challenge to isolate and characterize the effective components of centipedes due to limited peptide purification technologies for animal-derived *** To purify,characterize,and synthesize the bioactive components with the strongest antihepatoma activity from centipedes and determine the antihepatoma *** An antihepatoma peptide(scolopentide)was isolated and identified from the centipede scolopendra subspinipes mutilans using a combination of enzymatic hydrolysis,a Sephadex G-25 column,and two steps of high-performance liquid chromatography(HPLC).Additionally,the CCK8 assay was used to select the extracted fraction with the strongest antihepatoma *** molecular weight of the extracted scolopentide was characterized by quadrupole time of flight mass spectrometry(QTOF MS),and the sequence was matched by using the Mascot search *** on the sequence and molecular weight,scolopentide was synthesized using solid-phase peptide synthesis *** synthetic scolopentide was confirmed by MS and *** antineoplastic effect of extracted scolopentide was confirmed by CCK8 assay and morphological changes again in *** antihepatoma effect of synthetic scolopentide was assessed by the CCK8 assay and Hoechst staining in vitro and tumor volume and tumor weight in *** the tumor xenograft experiments,qualified model mice(male 5-week-old BALB/c nude mice)were randomly divided into 2 groups(n=6):The scolopentide group(0.15 mL/d,via intraperitoneal injection of synthetic scolopentide,500 mg/kg/d)and the vehicle group(0.15 mL/d,via intraperitoneal injection of normal saline).The mice were euthanized by cervical dislocation after 14 d of con
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