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Basal Serum Anti-Miillerian Hormone Level as a Predictor of Clinical Outcomes in Freezing-all Embryo Transfer Program

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Current Medical Science 2018年第5期38卷 861-867页

作者： xiao-lan LI Rui HUANG Cong FANG xiao-yan liang Reproductive Medicine Research Center Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou 510655China

As a novel biomarker,there is inconsistent evidence regarding the association between anti-Miillerian hormone (AMH) and live birth rate in freezing-all embryo transfer cycles.We aim to assess the prognostic effect of baseline AMH on clinical outcomes,especially live birth rate in freezing-all embryo transfer cycles.A total of 828 non-polycystic ovary patients that underwent their first frozen-thawed embryo transfers in our center between January 2010 and January 2015 were recruited in this retrospective analysis.Patients were stratified into three groups based on their baseline AMH concentration:low AMH group (5.8 ng/mL).The results showed that low AMH level was associated With adverse clinical outcomes.The differences in implantation rate (21.9% vs.43.2% vs.58.8%,P<0.001),clinical pregnancy rate (32.0% vs.55.2% vs.65.7%, P<0.001),live birth delivery rate (21.8% vs.43.6% vs.52.7%,P<0.001)and miscarriage rate (31.8% vs.17.5% vs.15.4%,P=0.014)among the three groups were statistically significant.After adjusting confounders (i.***.age,baseline FSH level,AFC,endometrium thickness,endometriurn preparation protocols,number of embryos transferred,etiologies of infertility),differences in live birth rate,clinical pregnancy rate and implantation rate between groups remained significant.The further age subgroup analysis demonstrated that low AMH concentration was significantly associated with poor outcomes both in young and advanced patients.The area under the curve for serum AMH,age,AFC and FSH were 0.635,0.634,0.615 and 0.543 respectively,for predicting live birth.In conclusion, baseline AMH was an independent prognostic factor of live birth rate of freezing-all embryo transfers,but its predictive value on live birth rate was of limited clinical value.

关键词： anti-Miillerian hormone freezing-all embryo transfer live birth rate

Epidemiological Survey and Risk Factor Analysis of Recurrent Spontaneous Miscarriages in Infertile Women at Large Infertility Centers

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Chinese Medical Journal 2017年第17期130卷 2056-2062页

作者： Hai-yan Wang Jie Qiao xiao-Xi Sun Shu-Yu Wang xiao-yan liang Yun Sun Feng-Hua Liu Reproductive Medical Center Department of Obstetrics and Gynecology Peking University Third Hospital Beijing 100191 China Shanghai Ji Ai Diagnosis and Treatment Center for Genetics and Infertility Shanghai 200011 China Reproductive Medical Center Beijing Obstetrics and Gynecology Hospital Beijing 100006 China Reproductive Medical Center The Sixth Affiliated Hospital Sun Yat-sen University Guangzhou Guangdong 510665 China Reproductive Medical Center Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital Shanghai 200011 China Reproductive Medical Center Maternal and Child Health Hospital of Guangdong Province Guangzhou Guangdong 511400 China

Background：A higher frequency of spontaneous miscarriage has been observed in infertile couples,and there is a higher prevalence of infertility among patients with a history of recurrent spontaneous miscarriages （RSMs;〉2 miscarriages）.This study aimed to determine the proportion of infertile patients with RSM and examine risk factors associated in patients with RSM being treated with assisted reproductive technologies.Methods：This cross-sectional observational study was conducted at six reproductive medicine centers in three cities of China.Data of 751 patients with at least one spontaneous miscarriage were analyzed.Demographic data and etiological factors associated with infertility were compiled and compared between patients with a single spontaneous miscarriage （SSM） and those with RSM.Results：Two hundred （26.6%,95% confidence interval [CI]：23.50-29.95%） patients experienced RSMs and 551 （73.4%） had a single miscarriage.The odds of RSM increased with increasing age （odds ratio [OR] =1.06）,uterine disorders （OR =2.09）,endocrine disorders （OR =2.48）,and immune disorders （OR =2.98）.Higher education level,masters or above,and a pelvic cavity disorder were associated with lower risk of RSM （OR =0.27 and 0.46,respectively）.Late spontaneous miscarriages were more frequent in patients with RSM than in those with a SSM （31.5% vs.14.2%,respectively,P 〈 0.001） and were associated with a history of uterine cavity procedures （OR =2.095） and cervical factors related to infertility （OR =4.136,95% CI：1.012-16.90）.Conclusions：Compared to patients with only a SSM,the conditions of patients with RSM are more complicated.To increase the success rate of assisted reproductive technology,factors including uterus cavity adhesion,cervical relaxation,endocrine disorders,and immune disorders should be treated before assisted reproduction is initiated.These data may provide treatment guidance for infertile patients with a history of RSM.

关键词： Assisted Reproductive Technology Epidemiological Survey Infertility Recurrent Spontaneous Miscarriage Risk Factor

Designing salt stress-resilient crops:Current progress and future challenges

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Journal of Integrative Plant Biology 2024年第3期66卷 303-329页

作者： xiaoyan liang Jianfang Li Yongqing yang Caifu Jiang yan Guo State Key Laboratory of Plant Environmental Resilience College of Biological SciencesChina Agricultural UniversityBeijing 100094China State Key Laboratory of Nutrient Use and Management College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100194China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100094China Frontiers Science Center for Molecular Design Breeding China Agricultural UniversityBeijing 100193China

Excess soil salinity affects large regions of land and is a major hindrance to crop production worldwide.Therefore,understanding the molecular mechanisms of plant salt tolerance has scientific importance and practical significance.In recent decades,studies have characterized hundreds of genes associated with plant responses to salt stress in different plant species.These studies have substantially advanced our molecular and genetic understanding of salt tolerance in plants and have introduced an era of molecular design breeding of salt-tolerant crops.This review summarizes our current knowledge of plant salt tolerance,emphasizing advances in elucidating the molecular mechanisms of osmotic stress tolerance,salt-ion transport and compartmentalization,oxidative stress tolerance,alkaline stress tolerance,and the trade-off between growth and salt tolerance.We also examine recent advances in understanding natural variation in the salt tolerance of crops and discuss possible strategies and challenges for designing salt stress-resilient crops.We focus on the model plant Arabidopsis(Arabidopsis thaliana)and the four most-studied crops:rice(Oryza sativa),wheat(Triticum aestivum),maize(Zea mays),and soybean(Glycine max).

关键词： molecular design breeding salt stress-resilient crop salt tolerance

Logistic Regression Analysis of Risk Factors Associated with Spontaneous Abortion after In Vitro Fertilization/Intracytoplasmic Sperm Injection-Embryo Transfer in Polycystic Ovary Syndrome Patients

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Reproductive and Developmental Medicine 2018年第2期2卷 105-110页

作者： xiao-Lan Li Rui Huang Cong Fang yan-Fang Wang xiao-yan liang Reproductive Medicine Center The Sixth Affiliated Hospital of Sun Yat‑Sen UniversityGuangzhou 510655China

Objective:To analyze spontaneous abortion rate and its associated risk factors in patients with polycystic ovary syndrome(PCOS)after in vitro fertilization/intracytoplasmic sperm injection-embryo transfer(IVF/ICSI-ET).Methods:This was a retrospective cohort study on 2,231 PCOS patients undergoing IVF/ICSI treatment.For comparison,we enrolled another 2,231 patients with tubular factors in control group.Spontaneous abortion rate and chromosomal abnormality rate were compared between the two groups.Furthermore,clinical data were compared between the patients with spontaneous abortion and those with ongoing pregnancy using univariate and multivariate analysis for risk factors of spontaneous abortion in PCOS patients.Results:Patients in the PCOS group had significantly higher spontaneous abortion rate(24.15%)than that in the control group(12.75%,P<0.001).Chromosomal abnormality rate was significantly lower in the PCOS group(36.05%,31/86)than in the control group(55.56%,50/90,P=0.009).In the PCOS group,patients with spontaneous abortion had significantly elder age,higher body mass index(BMI),and homeostasis model assessment for insulin resistance(HOMA-IR)than those with ongoing pregnancy.The logistic regression analysis confirmed that the age,BMI,and HOMA-IR were the risk factors of spontaneous abortion in the PCOS patients.There were no significant differences between groups in the type of cycles,the quality of embryos transferred,the number of embryos transferred,fasting blood glucose,fasting insulin,and so on.Conclusions:Compared with non-PCOS patients,PCOS patients had higher spontaneous abortion rate,but the chromosomal abnormality was not a major factor for the high spontaneous abortion rate in the PCOS patients.Age,BMI,and HOMA-IR were risk factors for spontaneous abortion after IVF/ICSI-ET in PCOS patients.

关键词： Polycystic Ovary Syndrome Risk Factors Spontaneous Abortion

Allograft pretreatment for the repair of sciatic nerve defects: green tea polyphenols versus radiation

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Neural Regeneration Research 2015年第1期10卷 136-140页

作者： Sheng-hu Zhou Ping Zhen Shen-song Li xiao-yan liang Ming-xuan Gao Qi Tian Xu-sheng Li Orthopedics Center Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA

Pretreatment of nerve allografts by exposure to irradiation or green tea polyphenols can elimi- nate neuroimmunogenicity, inhibit early immunological rejection, encourage nerve regeneration and functional recovery, improve tissue preservation, and minimize postoperative infection. In the present study, we investigate which intervention achieves better results. We produced a 1.0 cm sciatic nerve defect in rats, and divided the rats into four treatment groups： autograft, fresh nerve allograft, green tea polyphenol-pretreated （1 mg/mL, 4~C） nerve allograft, and irradiation-pre- treated nerve allograft （26.39 Gy/min for 12 hours; total 19 kGy）. The animals were observed, and sciatic nerve electrophysiology, histology, and transmission electron microscopy were carried out at 6 and 12 weeks after grafting. The circumference and structure of the transplanted nerve in rats that received autografts or green tea polyphenol-pretreated nerve allografts were similar to those of the host sciatic nerve. Compared with the groups that received fresh or irradiation-pre- treated nerve allografts, motor nerve conduction velocity in the autograft and fresh nerve allograft groups was greater, more neurites grew into the aUografts, Schwann cell proliferation was evident, and a large number of new blood vessels was observed; in addition, massive myelinated nerve fibers formed, and abundant microfilaments and microtubules were present in the axoplasm. Our findings indicate that nerve allografts pretreated by green tea polyphenols are equivalent to trans- planting autologous nerves in the repair of sciatic nerve defects, and promote nerve regeneration. Pretreatment using green tea polyphenols is better than pretreatment with irradiation.

关键词： nerve regeneration peripheral nerve injury allografi green tea polyphenols irradiation sciatic nerve transplantation nerve defects nerve repair alternative nerual regeneration

Establishing a nonlethal and efficient mouse model of male gonadotoxicity by intraperitoneal busulfan injection

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Asian Journal of Andrology 2020年第2期22卷 184-191页

作者： Yun Xie Cun-Can Deng Bin Ouyang Lin-yan Lv Jia-Hui Yao Chi Zhang Hai-Cheng Chen xiao-yan liang Xiang-Zhou Sun Chun-Hua Deng Gui-Hua Liu Department of Andrology The First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510000China Guangdong Provincial Key Laboratory of Orthopedics and Traumatology Guangzhou 510000China Reproductive Centre The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou 510000China Gastrointestinal Diseases Research Institute of Guangdong Province The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou 510000China Department of Andrology Guangzhou First People’s HospitalSchool of MedicineSouth China University of TechnologyGuangzhou 510000China

An ideal animal model of azoospermia would be a powerful tool for the evaluation of spermatogonial stem cell(SSC)transplantation.Busulfan has been commonly used to develop such a model,but 30%–87%of mice die when administered an intraperitoneal injection of 40 mg kg^?1.In the present study,hematoxylin and eosin staining,Western blot,immunofluorescence,and quantitative real-time polymerase chain reaction were used to test the effects of busulfan exposure in a mouse model that received two intraperitoneal injections of busulfan at a 3-h interval at different doses(20,30,and 40 mg kg^?1)on day 36 or a dose of 40 mg kg^?1 at different time points(0,9,18,27,36,and 63 days).The survival rate of the mice was 100%.When the mice were treated with 40 mg kg^?1 busulfan,dramatic SSC depletion occurred 18 days later and all of the germ cells were cleared by day 36.In addition,the gene expressions of glial cell line-derived neurotrophic factor(GDNF),fibroblast growth factor 2(FGF2),chemokine(C-X-C Motif)ligand 12(CXCL12),and colony-stimulating factor 1(CSF1)were moderately increased by day 36.A 63-day,long-term observation showed the rare restoration of endogenous germ cells in the testes,suggesting that the potential period for SSC transplantation was between day 36 and day 63.Our results demonstrate that the administration of two intraperitoneal injections of busulfan(40 mg kg^?1 in total)at a 3-h interval to mice provided a nonlethal and efficient method for recipient preparation in SSC transplantation and could improve treatments for infertility and the understanding of chemotherapy-induced gonadotoxicity.

关键词： azoospermia busulfan gonadotoxicity infertility recipient preparation spermatogonial stem cell

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荒漠区沥青路面拱胀病害机理及影响因素分析

公路交通科技 2024年第4期41卷 31-41页

作者：林宇坤宋玲刘杰闫晓亮朱世煜石河子大学水利建筑工程学院新疆石河子832003 新疆交通规划勘察设计研究院有限公司新疆乌鲁木齐830006 新疆交投阿乌高速公路有限责任公司新疆乌鲁木齐830006 长安大学公路学院陕西西安710064

针对阿勒泰荒漠区S21阿乌高速出现的沥青路面拱胀病害问题,对其机理及影响因素进行分析。经现场调查与室内试验,分析沥青路面拱胀病害成因。以调查结果为基础,运用Kirchhoff薄板理论与有限积分变换法理论推导基层拱胀临界应力计算公式,... 详细信息

针对阿勒泰荒漠区S21阿乌高速出现的沥青路面拱胀病害问题,对其机理及影响因素进行分析。经现场调查与室内试验,分析沥青路面拱胀病害成因。以调查结果为基础,运用Kirchhoff薄板理论与有限积分变换法理论推导基层拱胀临界应力计算公式,分析沥青路面拱胀病害力学机制,确定基层拱胀临界应力值。应用Abaqus建立道路有限元模型,分析基层施工温度、膨胀系数、回弹模量及路表最高温度等因素对基层纵向最大应力与路面最大拱起高度的影响,并基于基层拱胀临界应力值给出相应建议。研究认为:S21阿乌高速沥青路面拱胀病害是水泥稳定砾石基层在过大的温度应力作用下发生拱起破坏的结果。考虑实际道路情况,S21阿乌高速水泥稳定砾石基层拱胀临界应力值为3.49 MPa。当基层纵向最大应力大于基层拱胀临界应力值时,基层将会发生拱起失稳破坏。基层施工温度、膨胀系数及路表最高温度均为基层纵向最大应力与路面最大拱起高度的敏感因素,而基层回弹模量对路面最大拱起高度无明显影响。建议将基层膨胀系数控制在2.8×10^(-5)℃^(-1)以内,基层回弹模量控制在1400~2200 MPa以内。

关键词：道路工程沥青路面拱胀病害薄板理论水泥稳定砾石基层有限元分析

MP-3 microperimeter in early primary open angle glaucoma with a new pattern

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International Journal of Ophthalmology(English edition) 2024年第5期17卷 861-868页

作者： Shuang liang Li-Tao Wang xiao-Li Liu Jia-liang Duan Dan-yan Liu Department of Ophthalmology of the Second Hospital of Hebei Medical University Shijiazhuang 050000Hebei ProvinceChina Department of Ophthalmology Shijiazhuang People’s HospitalShijiazhuang 050000Hebei ProvinceChina

AIM:To investigate macular microperimetry in patients with early primary open angle glaucoma(POAG)using a new custom-made pattern,and analyze the characteristics of macular sensitivity.METHODS:This case-control study included 38 patients with POAG,who were divided into pre-perimetric glaucoma(18 eyes of 18 patients),early-stage(20 eyes of 20 patients),and control(20 eyes of 20 patients)groups.All subjects underwent standard 24-2 humphrey visual field test.An MP-3 microperimeter with a new custom-made pattern(28 testing points distributed in four quadrants,covering the central 10°of the retina)was used to evaluate macular sensitivity.Ganglion cell complex(GCC)thicknesses were examined using an RS-3000 Advance OCT system.The features of structure and function were analysed per quadrant.RESULTS:The pre-perimetric glaucoma group had significantly lower inferior hemifield macular sensitivity compared to controls(P<0.05).The early-stage POAG group had significantly lower average,inferior hemifield,inferonasal,and inferotemporal mean sensitivities compared to the pre-perimetric glaucoma group(P<0.05),and lower macular sensitivity in all sectors compared to controls(P<0.05).Regarding GCC thickness,all sectors in the early-stage POAG group became thinner compared to those in controls(P<0.05);whereas all sectors in the early-stage POAG group,except the superonasal quadrant,became thinner compared to those in the pre-perimetric glaucoma group(P<0.05).Macular sensitivity and GCC thickness were significantly associated in each sector.The inferotemporal quadrant had the highest correlation coefficients(0.840).The structure-function relationship for the inferonasal and inferotemporal sectors was stronger compared to the corresponding superior sectors.CONCLUSION:Microperimetry reveals variations in macular sensitivity in patients with early glaucoma earlier than conventional perimetry,particularly in pre-perimetric glaucoma cases in which it might be undetectable by conventional methods.The new custom-made pattern may improve th

关键词： early primary open angle glaucoma macular sensitivity microperimetry optical coherence tomography ganglion cell complex

Large animal models for Huntington's disease research

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Zoological Research 2024年第2期45卷 275-283页

作者： Bofeng Han Weien liang xiao-Jiang Li Shihua Li Sen yan Zhuchi Tu Guangdong-Hongkong-Macao CNS Regeneration Institute of Jinan University Key Laboratory of CNS Regeneration(Jinan University)-Ministry of EducationGuangdong Key Laboratory of Non-Human Primate ResearchGuangzhouGuangdong 510632China Department of Pathophysiology School of MedicineJinan UniversityGuangzhouGuangdong 510632China

Huntington'sdisease(HD)isahereditary neurodegenerative disorder for which there is currently no effectivetreatmentavailable.Consequently,the development of appropriate disease models is critical to thoroughly investigate disease progression.The genetic basis of HD involves the abnormal expansion of CAG repeats in the huntingtin(HTT)gene,leading to the expansion of a polyglutamine repeat in the HTT protein.Mutant HTT carrying the expanded polyglutamine repeat undergoes misfolding and forms aggregates in the brain,which precipitate selective neuronal loss in specific brain regions.Animal models play an important role in elucidating the pathogenesis of neurodegenerative disorders such as HD and in identifying potential therapeutic targets.Due to the marked species differences between rodents and larger animals,substantial efforts have been directed toward establishing large animal models for HD research.These models are pivotal for advancing the discovery of novel therapeutic targets,enhancing effective drug delivery methods,and improving treatment outcomes.We have explored the advantages of utilizing large animal models,particularly pigs,in previous reviews.Since then,however,significant progress has been made in developing more sophisticated animal models that faithfully replicate the typical pathology of HD.In the current review,we provide a comprehensive overview of large animal models of HD,incorporating recent findings regarding the establishment of HD knock-in(KI)pigs and their genetic therapy.We also explore the utilization of large animal models in HD research,with a focus on sheep,non-human primates(NHPs),and pigs.Our objective is to provide valuable insights into the application of these large animal models for the investigation and treatment of neurodegenerative disorders.

关键词： Huntington's disease Large animal models Sheep Non-human primates Transgenic pigs