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Chemical Research in Chinese Universities 2011年第4期27卷 635-640页

作者： YIN Yu-he NIU Xue SUN BO TENG Guo-sheng ZHAO Yun-hui wu cong-mei College of Chemistry and Life Science Changchun University of Technology Changchun 130012 P. R. China College of Life Science Jilin University Changchun 130012 P. R. China

When devoured by macrophages,Mycobacterium tuberculosis remains persistent in macrophages and gains energy through the glyoxylate bypass to maintain its long-term existence in host *** it is possible to stop persistent infections by interdicting the glyoxylate bypass in which the isocitrate lyase（ICL） is the key rate-limiting enzyme and a persistence *** is the target of anti-TB（TB：tubercular） drugs,which could screen ICL out and effectively inhibit the activity of ICL in Mycobacterium tuberculosis,and because of this,anti-TB drugs can be used to kill persistent Mycobacterium *** this study,the ICL gene of the Mycobacterium tuberculosis H37Rv was cloned successfully and recombinant protein with bioactivity was obtained through the enzyme characteristic *** specific activity of the recombined ICL is 24μmol·mg-1·*** recombined ICL protein was used as the target,and phages which can specifically combine to ICL were screened in the phage 7 peptide *** to the results of the ELISA and DNA sequence detection,eventually three 7-peptide chains were *** the peptide chains were reacted with ICL,respectively,to detect their inhibitory effects on *** results show that all the three 7-peptide chains possessed varying inhibitory effects on the activity of *** study provided lead compounds for the research and development of new peptide anti-TB drugs.

关键词： Mycobacterium tuberculosis Isocitrate lyase Gene expression Phage peptide library Peptide inhibitor

Dome Combustion Hot Blast Stove for Huge Blast Furnace

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Journal of Iron and Steel Research International 2012年第9期19卷 1-7页

作者： ZHANG Fu-ming , MAO Qing-wu , mei cong-hua , LI Xin , HU Zu-rui ( Beijing Shougang International Engineering Technology Co Ltd , Beijing 100043 , China ) Beijing Shougang International Engineering Technology Co Ltd Beijing 100043 China

In Shougang Jingtang 5 500m 3 huge blast furnace ( BF ) design , dome combustion hot blast stove ( DCHBS ) technology is *** process is optimized and integrated , and reasonable hot blast stove ( HBS ) technical parameters are *** model is established and adopted by computational fluid dynamics ( CFD ) .The transmission theory is studied for hot blast stove combustion and gas flow , and distribution results of HBS velocity field , CO density field and temperature field are *** test model and hot trail unit are established , and the numeral calculation result is verified through test and investigation.3-D simulation design is *** process flow and process layout are optimized and *** air two-stage high temperature preheating technology is designed and *** sets of small size DCHBSs are adopted to preheat the combustion air to 520-600℃.With the precondition of BF gas combustion , the hot blast stove dome temperature can exceed 1 420 ℃. According to DCHBS technical features , reasonable refractory structure is *** technical measures are adopted to prevent hot blast stove shell intercrystalline stress *** blast stove hot pipe and lining system are optimized and *** blowing in , the blast temperature keeps increasing , and the monthly average blast temperature reaches 1 300℃ when burning single BF gas.

关键词： dome combustion hot blast stove high blast temperature ceramic burner high temperature combustion air preheating CFD

Embryonic Stem Cells-loaded Gelatin Microcryogels Slow Progression of Chronic Kidney Disease

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Chinese Medical Journal 2016年第4期129卷 392-398页

作者： Xiao-Dong Geng Wei Zheng cong-mei wu Shu-Qiang Wang Quan Hong Guang-Yan Cai Xiang-mei Chen Di wu Department of Nephrology Chinese People's Liberation Army General Hospital Chinese People's Liberation Army Institute of Nephrology State Key Laboratory of Kidney Diseases National Clinical Research Center for Kidney Diseases Beijing Key Laboratory of Kidney Disease Beijing 100853 China Department of Biotechnology School of Chemistry and Life Science Changchun University of Technology Changchun Jilin 130012 China

Background： Chronic kidney disease （CKD） has become a public health *** interventions to slow or prevent disease progression are urgently *** this setting, cell therapies associated with regenerative effects are attracting increasing *** evaluated the effect of embryonic stem cells （ESCs） on the progression of ***： Adult male Sprague-Dawley rats were subjected to 5/6 *** used pedicled greater omentum flaps packing ESC-loaded gelatin microcryogets （GMs） on the 5/6 nephrectomized *** viability of ESCs within the GMs was detected using in vio two-photon fluorescence confocal *** were sacrificed after 12 *** injury was evaluated using serum creatinine, urea nitrogen, 24 h protein, renal pathology, and tubular injury score *** damage was evaluated by periodic acid-Schiff and Masson trichrome ***： In vitro, ESCs could be automatically loaded into the *** cell distribution, good cell attachment, and viability were achieved from day 1 to 7 in *** 12 weeks, in the pedicled greater omentum flaps packing ESC-loaded GMs on 5/6 nephrectomized rats group, the plasma urea nitrogen levels were 26% lower than in the right nephrectomy group, glomerulosclerosis index was 62% lower and tubular injury index was 40% lower than in the 5/6 nephrectomized rats group without ***： In a rat model of established CKD, we demonstrated that the pedicled greater omentum flaps packing ESC-loaded GMs on the 5/6 nephrectomized kidney have a long-lasting therapeutic rescue function, as shown by the decreased progression of CKD and reduced glomerular injury.

关键词： Chronic Renal Insufficiency Embryonic Stem Cells Gelatin Microcryogels Regenerative Medicine

Sky location of massive black hole binaries in the foreground of Galactic white dwarf binaries

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 97-108页

作者： Pan Guo Hong-Bo Jin cong-Feng Qiao Yue-Liang wu The International Centre for Theoretical Physics Asia-Pacific University of Chinese Academy of Sciences The School of Physical Sciences University of Chinese Academy of Sciences Institute for Frontiers in Astronomy and Astrophysics Beijing Normal University National Astronomical Observatories Chinese Academy of Sciences Institute of Theoretical Physics Chinese Academy of Sciences

For space-based gravitational wave （GW） detection,the main noise source for massive black hole binaries （MBHBs） is attributed to approximately 10～7double white dwarf binaries in the *** a GW source,the amplitude of the detector response,recorded by a space-based gravitational wave detector,exhibits a modulation effect with a year period when observing the source from various orbital *** the adverse conditions mentioned above,where there is a strong foreground noise and annual modulation in the signals,we employed the wavelet transform and the strong-amplitude relevant orbital position search methods,which allows the weak MBHB sources to achieve higher locating *** detail,for two MBHB sources of lower intensity,the precision of luminosity distance,represented by the ratio ?DL/DLat the 95%confidence level,is enhanced by factors of ～*** angular resolutions,denoted by??s,are enhanced by a factor of ～*** improvements increase the number of detectable GW sources,facilitate multi-messenger follow-up observations and provide constraints on the cosmological constant.

关键词： space-based gravitational wave detection gravitational waves massive black hole binaries

pEgr-TNFα质粒的构建及基因-放射治疗小鼠黑色素瘤的实验研究

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中华肿瘤杂志 2004年第3期26卷 143-145页

作者：吴丛梅李修义汕头大学医学院生物学教研室广东515031

目的　研究pEgr TNFα重组质粒的构建及其联合放射治疗抑制小鼠黑色素瘤的效果和对免疫系统的影响。方法　将TNFαcDNA插入pcDNA3.1质粒的多克隆位点 ,用Egr 1的启动子替代pcDNA3.1质粒的CMV启动子 ,构建成pEgr TNFα重组质粒 ;建立小鼠... 详细信息

目的　研究pEgr TNFα重组质粒的构建及其联合放射治疗抑制小鼠黑色素瘤的效果和对免疫系统的影响。方法　将TNFαcDNA插入pcDNA3.1质粒的多克隆位点 ,用Egr 1的启动子替代pcDNA3.1质粒的CMV启动子 ,构建成pEgr TNFα重组质粒 ;建立小鼠B16黑色素瘤动物模型 ,肿瘤局部注射脂质体包裹的质粒 ,在不同时间测量肿瘤体积 ,并检测照后 15d部分免疫学指标。结果　pEgr TNFα基因放射联合治疗后第 3～ 15天 ,肿瘤生长速率明显低于单纯照射组、单纯基因治疗组和空质粒对照组。小鼠脾脏NK细胞毒活性、IL 2分泌活性、腹腔巨噬细胞TNFα和IL 1β活性均明显高于单纯放疗组。结论　pEgr TNFα基因放射联合治疗抗肿瘤作用明显优于单纯放疗组和单纯基因治疗组 ,同时可以减轻放疗对机体免疫功能的损伤。

关键词： pEgr-TNFα重组质粒基因治疗放射治疗黑色素瘤小鼠免疫功能

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小分子化合物S1诱导PC3细胞凋亡的作用

吉林大学学报（医学版） 2008年第3期34卷 361-363页

作者：吴丛梅杨清金顺子长春工业大学生物工程学院吉林长春130012 吉林大学第一医院检验科吉林长春130021 吉林大学公共卫生学院卫生部放射生物学重点实验室吉林长春130021

目的：研究小分子化合物8-氧-8H-苊并[1，2-b]吡咯-9-腈的氨基或卤素取代衍生物（S1）对人雄性激素非依赖型前列腺癌PC3细胞的作用及机制。方法：常规培养PC3细胞，分为10．00、5．00、1．00、0．50、0．10、0．05和0．01μmol·L^-1 S... 详细信息

目的：研究小分子化合物8-氧-8H-苊并[1，2-b]吡咯-9-腈的氨基或卤素取代衍生物（S1）对人雄性激素非依赖型前列腺癌PC3细胞的作用及机制。方法：常规培养PC3细胞，分为10．00、5．00、1．00、0．50、0．10、0．05和0．01μmol·L^-1 S1化合物组，同时设培养肿瘤细胞对照组和抗癌药物环磷酰胺（5．00 μmol·L^-1）对照组，采用MTT法检测PC3细胞增殖抑制率，流式细胞术检测诱导PC3细胞凋亡作用，Caspase3、8和9试剂盒检测细胞凋亡途径。结果：0．10～10．00μmol·L^-1 S1化合物组，PC3细胞生长抑制率明显高于环磷酰胺对照组（P〈O．01）；5．00和10．00μmol·L^-1 S1化合物组PC3细胞凋亡率明显高于培养肿瘤细胞对照组（P〈0．01）；10．00μmol·L^-1 S1化合物作用于PC3细胞后不同时间（1、2、4及6h）细胞Caspase 3和Caspase 9活性均显著高于培养肿瘤细胞对照组（P〈0．01），Caspase 8活性未见明显改变。结论：S1化合物能够通过诱导PC3细胞凋亡而导致细胞死亡，其作用机制是线粒体途径，进一步证实S1化合物作为肿瘤治疗药物的可行性。

关键词： PC3细胞系细胞凋亡机制

Crystallization Modulation and Holistic Passivation Enables Efficient Two‑Terminal Perovskite/CuIn(Ga)Se_(2)Tandem Solar Cells

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Nano-Micro Letters 2025年第1期17卷 205-215页

作者： cong Geng Kuanxiang Zhang Changhua Wang Chung Hsien wu Jiwen Jiang Fei Long Liyuan Han Qifeng Han Yi‑Bing Cheng Yong Peng State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070People’s Republic of China Triumph Photovoltaic Materials Co. Ltd.No.1001 Yannan AvenueHigh‑Tech Development DistrictBengbuAnhui 233000People’s Republic of China College of Materials Science and Engineering Guilin University of TechnologyGuilin 541004People’s Republic of China State Key Laboratory of Metal Matrix Composites Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

Two-terminal(2-T)perovskite(PVK)/CuIn(Ga)Se_(2)(CIGS)tandem solar cells(TSCs)have been considered as an ideal tandem cell because of their best bandgap matching regarding to Shockley–Queisser(S–Q)***,the nature of the irregular rough morphology of commercial CIGS prevents people from improving tandem device *** this paper,D-homoserine lactone hydrochloride is proven to improve coverage of PVK materials on irregular rough CIGS surfaces and also passivate bulk defects by modulating the growth of PVK *** addition,the minority carriers near the PVK/C60 interface and the incompletely passivated trap states caused interface recombination.A surface reconstruction with 2-thiopheneethylammonium iodide and N,N-dimethylformamide assisted passivates the defect sites located at the surface and grain ***,LiF is used to create this field effect,repelling hole carriers away from the PVK and C60 interface and thus reducing *** a result,a 2-T PVK/CIGS tandem yielded a power conversion efficiency of 24.6%(0.16 cm^(2)),one of the highest results for 2-T PVK/CIGS TSCs to our *** validation underscores the potential of our methodology in achieving superior performance in PVK/CIGS tandem solar cells.

关键词： PVK/CIGS TSCs Irregular rough surfaces Modulating the growth PVK/C60 interface recombination

一种新型小分子化合物增强PC3细胞的辐射敏感性

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肿瘤防治研究 2009年第1期36卷 28-30页

作者：吴丛梅殷玉和吴德生长春工业大学生物工程学院 130012 汕头大学医学院生殖研究中心

目的检测小分子化合物8-氧-8H-苊并[1,2-b]吡咯-9-腈的氨基取代衍生物(S1)对前列腺癌细胞系PC3辐射敏感性的作用。方法采用流式细胞术分别检测不同剂量X射线和不同浓度S1化合物以及两者联合作用后,PC3细胞凋亡率的变化;采用Western blo... 详细信息

目的检测小分子化合物8-氧-8H-苊并[1,2-b]吡咯-9-腈的氨基取代衍生物(S1)对前列腺癌细胞系PC3辐射敏感性的作用。方法采用流式细胞术分别检测不同剂量X射线和不同浓度S1化合物以及两者联合作用后,PC3细胞凋亡率的变化;采用Western blot方法检测S1作用后不同时间Bcl-2蛋白在PC3细胞中的表达变化。结果X射线诱导PC3细胞凋亡具有剂量依赖特性,2Gy、5GyX射线照射未发现明显的凋亡,10Gy、20Gy照射组有明显的凋亡产生(P<0.01);S1化合物能够显著诱导PC3细胞凋亡,并具有剂量依赖特性,其中5μmol/L和10μmol/L组凋亡率明显高于对照组(P<0.01);S1作用后2～12hPC3细胞中Bcl-2蛋白表达逐渐减低;S1作用后,分别给予PC3细胞2Gy和8GyX射线照射,细胞凋亡率明显高于单独照射组和单独S1作用组(P<0.01)。结论S1能够增强PC3细胞对X射线的敏感性,可望为临床提高前列腺癌放疗效果,降低辐射损伤提供新的治疗方案。

关键词：前列腺癌放疗增敏

PTEN基因稳定转染联合辐射对肿瘤细胞周期进程及G_1期激酶抑制蛋白P27^(kip1)表达的影响

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吉林大学学报（医学版） 2007年第2期33卷 195-199页

作者：田梅吴丛梅刘林林朴春姬李修义吉林大学公共卫生学院卫生部放射生物学重点实验室长春工业大学生物工程学院吉林长春130012 吉林大学第二医院放疗科吉林长春130041

目的:研究辐射诱导表达载体pEgr-hPTEN体外转染对人脑胶质瘤SHG-44细胞周期进程及G1期激酶抑制蛋白P27表达的影响。方法:脂质体包裹重组载体体外转染肿瘤细胞并经G418筛选稳定表达细胞株,光学显微镜和透射电镜观察稳定转染细胞形态,以We... 详细信息

目的:研究辐射诱导表达载体pEgr-hPTEN体外转染对人脑胶质瘤SHG-44细胞周期进程及G1期激酶抑制蛋白P27表达的影响。方法:脂质体包裹重组载体体外转染肿瘤细胞并经G418筛选稳定表达细胞株,光学显微镜和透射电镜观察稳定转染细胞形态,以Western blotting法检测PTEN基因的辐射诱导表达情况,应用流式细胞术研究稳定转染联合0~10Gy X-射线照射对胶质瘤细胞周期进程及P27kip1的表达。结果:稳定转染PTEN基因的SHG-44细胞PTEN蛋白的相对表达量可被辐射诱导增强,5Gy以内PTEN蛋白的相对表达量随照射剂量增加而增加;稳定转染细胞超微结构有明显的退行性改变,可见核内染色质趋边的类似早期凋亡的改变;稳定转染联合X-射线照射细胞周期发生明显改变,细胞从G1期到S期发生阻滞,细胞周期相关蛋白P27表达上调。结论:PTEN基因稳定转染联合辐射可诱导肿瘤细胞G1期阻滞,并与P27kip1表达上调有关。

关键词： pEgr-hPTEN X线神经胶质瘤 P27^kip1 细胞周期

辐射诱导pEgr-p16表达增强及其体外抑制B16黑色素瘤作用

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中华放射医学与防护杂志 2007年第3期27卷 235-238页

作者：周莉吴丛梅黄天华汕头大学医学院附属肿瘤医院妇瘤科广东515031 长春工业大学生物工程学院汕头大学医学院生物学教研室

目的研究pEgr-p16重组质粒在转染的黑色素瘤B16细胞中的辐射诱导表达特性,验证其联合放射治疗体外抑制B16细胞增殖的作用。方法将脂质体包裹的pEgr-p16重组质粒,转染B16细胞株;采用定量PCR方法检测不同剂量X射线诱导后p16的表达和同一剂... 详细信息

目的研究pEgr-p16重组质粒在转染的黑色素瘤B16细胞中的辐射诱导表达特性,验证其联合放射治疗体外抑制B16细胞增殖的作用。方法将脂质体包裹的pEgr-p16重组质粒,转染B16细胞株;采用定量PCR方法检测不同剂量X射线诱导后p16的表达和同一剂量X射线诱导下p16的表达时程,流式细胞数术检测细胞凋亡率的变化;MTT法检测pEgr-p16基因协同放射治疗后B16细胞的存活情况。结果pEgr-p16重组质粒转染B16细胞,不同剂量X射线均可诱导p16表达增强,为假照组的3.78-6.67倍（P〈0.01）;2GyX射线照射后,p16表达随时间延长而逐渐增强,在照射后72h达到最高值。p16基因联合放射可诱导B16细胞凋亡,凋亡率高于单纯照射组和单纯基因诱导组（P〈0.05-0.01）;转染pEgr-p16质粒的细胞经2GyX射线照射,8d后细胞数明显低于同时间其他实验组（P〈0.05-0.001）。结论pEgr-p16基因-放射联合治疗有明显的抑制黑色素瘤B16细胞生长的作用,其作用优于单纯给予射线或基因转染。

关键词： pEgr-p16基因辐射 B16细胞