Embryonic Stem Cells-loaded Gelatin Microcryogels Slow Progression of Chronic Kidney Disease

作     者：Xiao-Dong Geng Wei Zheng Cong-Mei Wu Shu-Qiang Wang Quan Hong Guang-Yan Cai Xiang-Mei Chen Di Wu 

作者机构：Department of Nephrology Chinese People's Liberation Army General Hospital Chinese People's Liberation Army Institute of Nephrology State Key Laboratory of Kidney Diseases National Clinical Research Center for Kidney Diseases Beijing Key Laboratory of Kidney Disease Beijing 100853 China Department of Biotechnology School of Chemistry and Life Science Changchun University of Technology Changchun Jilin 130012 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2016年第129卷第4期

页      面：392-398页

核心收录：

学科分类：08[工学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Hainan Province Medical Research Program 北京市科技新星计划项目 The Medical Technology Youth Training Project of PLA 国家自然科学基金 国家973计划 国家863计划 

主　　题：Chronic Renal Insufficiency Embryonic Stem Cells Gelatin Microcryogels Regenerative Medicine 

摘      要：Background： Chronic kidney disease （CKD） has become a public health *** interventions to slow or prevent disease progression are urgently *** this setting, cell therapies associated with regenerative effects are attracting increasing *** evaluated the effect of embryonic stem cells （ESCs） on the progression of ***： Adult male Sprague-Dawley rats were subjected to 5/6 *** used pedicled greater omentum flaps packing ESC-loaded gelatin microcryogets （GMs） on the 5/6 nephrectomized *** viability of ESCs within the GMs was detected using in vio two-photon fluorescence confocal *** were sacrificed after 12 *** injury was evaluated using serum creatinine, urea nitrogen, 24 h protein, renal pathology, and tubular injury score *** damage was evaluated by periodic acid-Schiff and Masson trichrome ***： In vitro, ESCs could be automatically loaded into the *** cell distribution, good cell attachment, and viability were achieved from day 1 to 7 in *** 12 weeks, in the pedicled greater omentum flaps packing ESC-loaded GMs on 5/6 nephrectomized rats group, the plasma urea nitrogen levels were 26% lower than in the right nephrectomy group, glomerulosclerosis index was 62% lower and tubular injury index was 40% lower than in the 5/6 nephrectomized rats group without ***： In a rat model of established CKD, we demonstrated that the pedicled greater omentum flaps packing ESC-loaded GMs on the 5/6 nephrectomized kidney have a long-lasting therapeutic rescue function, as shown by the decreased progression of CKD and reduced glomerular injury.