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Miniscope3D:optimized single-shot miniature 3D fluorescence microscopy

Light(Science & Applications) 2020年第1期9卷 367-379页

作者： Kyrollos Yanny Nick Antipa william liberti Sam Dehaeck Kristina Monakhova Fanglin Linda Liu Konlin Shen Ren Ng Laura Waller UCB/UCSF Joint Graduate Program in Bioengineering University of CaliforniaBerkeleyCA 94720USA Department of Electrical Engineering&Computer Sciences University of CaliforniaBerkeleyCA 94720USA TIPs Department Universite libre de Bruxelles(ULB)1050 BrusselsBelgium

Miniature fluorescence microscopes are a standard tool in systems ***,widefield miniature microscopes capture only 2D information,and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth ***,we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an optimized multifocal phase mask at the objective’s aperture *** the phase mask at the aperture stop significantly reduces the size of the device,and varying the focal lengths enables a uniform resolution across a wide depth *** phase mask encodes the 3D fluorescence intensity into a single 2D measurement,and the 3D volume is recovered by solving a sparsity-constrained inverse *** provide methods for designing and fabricating the phase mask and an efficient forward model that accounts for the fieldvarying aberrations in miniature *** demonstrate a prototype that is 17mm tall and weighs 2.5 grams,achieving 2.76μm lateral,and 15μm axial resolution across most of the 900×700×390μm^(3) volume at 40 volumes per *** performance is validated experimentally on resolution targets,dynamic biological samples,and mouse brain *** with existing miniature single-shot volume-capture implementations,our system is smaller and lighter and achieves a more than 2×better lateral and axial resolution throughout a 10×larger usable depth *** microscope design provides single-shot 3D imaging for applications where a compact platform matters,such as volumetric neural imaging in freely moving animals and 3D motion studies of dynamic samples in incubators and lab-on-a-chip devices.

关键词： enable resolution narrow

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Community Detection with the Weighted Parsimony Criterion

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Journal of Systems Science & Complexity 2015年第3期28卷 517-545页

作者： BETTINELLI Andrea HANSEN Pierre liberti Leo Dipartimento di Ingegneria dell’Energia Elettrica e Dell’Informazione Università di Bologna Viale del Risorgimento 2 40136 Bologna Italy Groupe d’études et de Recherche en Analyse des Décisions Hautes tudes Commerciales Montréal Canada LIX cole Polytechnique F-91128 Palaiseau France CNRS LIX Ecole Polytechnique 91128 Palaiseau France

Community detection in networks has been studied extensively in the last decade. Many criteria, expressing the quality of the partitions obtained, as well as a few exact algorithms and a large number of heuristics have been proposed. The parsimony criterion consists in minimizing the number of edges added or removed from the given network in order to transform it into a set of disjoint *** Zhang, Qiu and Zhang have proposed a weighted parsimony model in which a weight coefficient is introduced to balance the numbers of inserted and deleted edges. These authors propose rules to select a good value of the coefficient, use simulated annealing to find optimal or near-optimal solutions and solve a series of real and artificial instances. In the present paper, an algorithm is proposed for solving exactly the weighted parsimony problem for all values of the parameter. This algorithm is based on iteratively solving the problem for a set of given values of the parameter using a row generation algorithm. This procedure is combined with a search procedure to find all lowest breakpoints of the value curve(i.e., the weighted sum of inserted and deleted edges). Computational results on a series of artificial and real world networks from the literature are reported. It appears that several partitions for the same network may be informative and that the set of solutions usually contains at least one intuitively appealing partition.

关键词：网络社区简约加权检测标准生成算法权重系数模拟退火

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Effects of perinatal protein deprivation and recovery on esophageal myenteric plexus

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World Journal of Gastroenterology 2010年第5期16卷 563-570页

作者： Flavio M Greggio Ricardo BV Fontes Laura B Maifrino Patricia Castelucci Romeu Rodrigues de Souza Edson A liberti Autonomic Nervous System Laboratory (VQM) Department of Anatomy Instituto de Ciencias Biomedicas da Universidade de Sao Paulo 2415 Av Prof Lineu Prestes 05508-900 So Paulo Brazil

AIM:To evaluate effects of preand postnatal protein deprivation and postnatal recovery on the myenteric plexus of the rat esophagus. METHODS: Three groups of young Wistar rats (aged 42 d) were studied: normalfed (N42), proteindeprived (D42), and proteinrecovered (R42). The myenteric neurons of their esophagi were evaluated by histochemical reactions for nicotinamide adenine dinucleotide (NADH), nitrergic neurons (NADPH)diaphorase and acetylcholinesterase (AChE), immunohistochemical reaction for vasoactive intestinal polypeptide (VIP), and ultrastructural analysis by transmission electron ***: The cytoplasms of large and medium neurons from the N42 and R42 groups were intensely reactive for NADH. Only a few large neurons from the D42 group exhibited this aspect. NADPH detected in the D42 group exhibited low reactivity. The AChE reactivity was diffuse in neurons from the D42 and R42 groups. The density of large and small varicosities detected by immunohistochemical staining of VIP was low in ganglia from the D42 group. In many neurons from the D42 group, the double membrane of the nuclear envelope and the perinuclear cisterna were not detectable. NADH and NADPH histochemistry revealed no group differences in the prof ile of nerve cell perikarya (ranging from 200 to 400 μm2).CONCLUSION: Protein deprivation causes a delay in neuronal maturation but postnatal recovery can almost completely restore the normal morphology of myenteric neurons.

关键词： Esophagus Enteric nervous system Myenteric plexus Proteins Light microscopy Transmission electron microscopy

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Effects of protein deprivation and re-feeding on P2X_2 receptors in enteric neurons

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World Journal of Gastroenterology 2010年第29期16卷 3651-3663页

作者： Rúbia Misawa Priscila Azevedo Girotti Márcia Sanae Mizuno Edson Aparecido liberti John Barton Furness Patricia Castelucci Department of Anatomy Institute of Biomedical SciencesUniversity of So Paulo Department of Anatomy and Cell Biology University of Melbourne

AIM:To investigate the effects of malnutrition and refeeding on the P2X2 receptor,nitric oxide synthase(NOS),calretinin,calbindin and choline acetyltransferase(ChAT) in neurons of the rat ***:We analyzed the co-localization,numbers and sizes of P2X2-expressing neurons in relation to NOS-immunoreactive(IR),calbindin-IR,ChAT-IR,and calretinin-IR neurons of the myenteric and submucosal *** experimental groups consisted of:(1) rats maintained on normal feed throughout pregnancy until 42 d post-parturition(N);(2) rats deprived of protein throughout pregnancy and 42 d post-parturition(D);and(3) rats undernourished for 21 d post-parturition and then given a protein diet from days 22 to 42(DR).The myenteric and submucosal plexuses were evaluated by double labeling by immunohistochemical methods for P2X2 receptor,NOS,ChAT,calbindin and ***:We found similar P2X2 receptor immunoreactivity in the cytoplasm and surface membranes of myenteric and submucosal neurons from the N,D and DR *** labeling of the myenteric plexus demonstrated that approximately 100% of NOS-IR,calbindin-IR,calretinin-IR and ChAT-IR neurons in all groups also expressed the P2X2 *** the submucosal plexus,the calretinin-IR,ChAT-IR and calbindin-IR neurons were nearly all immunoreactive for the P2X2 *** the myenteric plexus,there was a 19% increase in numbers per cm2 for P2X2 receptor-IR neurons,64% for NOS-IR,84% for calretinin-IR and 26% for ChAT-IR neurons in the D *** spatial density of calbindin-IR neurons,however,did not differ among the three *** submucosal neuronal density increased for calbindin-IR,calretinin-IR and ChAT-IR *** average size of neurons in the myenteric plexus neurons in the D group was less than that in the controls and,in the re-fed rats;there was a 34% reduction in size only for the calretinin-IR ***:This work demonstrates that expression of the P2X2 receptor is present in inhibitory,intrins

关键词： Chemical coding Myenteric neurons Submucosal neurons Undernutrition

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Morphological effects of autoclaved diet on the myenteric neurons of rats

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World Journal of Gastroenterology 2011年第43期17卷 4799-4803页

作者： Patrícia O Gonalez Naianne K Clebis Renata B Mari Karina M Gagliardo Sandra R Stabille Haroldo G Faria Edson A liberti JoséRoberto Kfoury Jr Department of Surgery School of Veterinary Medicine and Animal SciencesUniversity of So Paulo Department of Morphology of the Centre of Biomedical Sciences Federal University of Rio Grande do Norte Department of Biology Paranaense University Department of Morphology MaringáState University Central Vivarium MaringáState University Department of Anatomy Biomedical Sciences InstituteUniversity of So Paulo

AIM:To evaluate the effect of autoclaved diet on the jejunum neurons of the myenteric plexus of rats during their ***:The experimental groups were made up of rats going through weaning whose mothers received either an autoclaved or a non-autoclaved diet during gestation and lactation,and rats that were fed the same diet as their mothers during the post-weaning *** order to measure the neurons'body profile and to quantify the number of neurons per area,preparations were stained by the nicotinamide adenine dinucleotide-diaphorase ***:No significant changes were observed in rats'body weight or in the number of neurons regardless of the diet used(P>0.05) .There was a decrease in the jejunum-ileum length in rats treated with an autoclaved diet(P<0.05) .An increase in the neuronal cross-sectional area was seen in rats that had received the autoclaved diet,an effect that was significant for animals undergoing *** addition,all observed factors showed significant differences when related to the age of the ***:The autoclaved diet did not alter the quantity of neurons,but increased their cell body area,suggesting changes similar to those observed in protein deficiency.

关键词：饮食治疗肌间神经丛非蒸压神经元大鼠烟酰胺腺嘌呤二核苷酸形态横截面积

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Effects of combined pre- and post-natal protein deprivation on the myenteric plexus of the esophagus of weanling rats:A histochemical,quantitative and ultrastructural study

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World Journal of Gastroenterology 2007年第26期13卷 3598-3604页

作者： Edson A liberti Ricardo BV Fontes Verginia M Fuggi Laura BM Maifrino Romeu R Souza Autonomic Nervous System Laboratory (VQM) Department of AnatomyInstitute of Biomedical SciencesUniversity of So Paulo So PauloBrazil

AIM: To evaluate the effects of protein deprivation on the myenteric plexus of the esophagus of weanling rats. METHODS: Pregnant female Wistar rats were divided into 2 groups: nourished (N),receiving normal diet,and undernourished (D),receiving a protein-deprived diet,which continued after birth. At twenty-one days of age,13 esophagi from each group were submitted to light microscopy and morphometrical analysis employing the NADH diaphorase,NADPH diaphorase and acetylcholinesterase techniques. Three other esophagi from each group were evaluated with transmission electron microscopy (TEM). RESULTS: In both the NADH- and the NADPH-reactive mounts,the neurons of the N mounts were more intensely stained,while in the D esophagi only the larger neurons were reactive. Many myenteric neurons of N were intensely reactive for AChE activity but only a few neurons of D exhibited these aspects. Ultrastructural analysis revealed that the granular reticulum of N showed large numbers of ribosomes aligned on the outer surface of its regularly arranged membrane while the ribosomes of D were disposed in clusters. The chromatin was more homogeneously scattered inside the neuron nucleus of N as well as the granular component of the nucleolus was evidently more developed in this group. Statistically significant differences between N and D groups were detected in the total estimated number of neurons stained by the NADPH technique. CONCLUSION: The morphological and quantitative data shows that feeding with protein-deprived diet in 21-d old rats induces a delay in the development of the myenteric neurons of the esophagus.

关键词：肠神经系统肠肌层营养学显微镜

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An Over-Moded TEM Cell System for <i>in vivo</i>Exposure at 2.45 GHz

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Journal of Electromagnetic Analysis and Applications 2012年第9期4卷 345-352页

作者： Alessandra Paffi Caterina Merla Micaela liberti Fabio Fratta Rosanna Pinto Giorgio A. Lovisolo Francesca Apollonio Italian Inter-University Centre of Electromagnetic Fields and Bio-Systems Department of Information Engineering Electronic and Telecommunication University of Rome “La Sapienza” Rome 00184 Italy Italian Inter-University Centre of Electromagnetic Fields and Bio-Systems Department of Information Engineering Electronic and Telecommunication University of Rome “La Sapienza” Rome 00184 Italy Italian Inter-University Centre of Electromagnetic Fields and Bio-Systems Italian National Agency for New Technologies Energy and Sustainable Economic Development Rome 00123 Italy.

A TEM cell designed to operate at 900 MHz for exposing small-restrained animals (e.g. mice) has been theoretically, numerically and experimentally characterized at 2.45 GHz, which is the central frequency of the WiFi protocol. This study aims at evaluating the influence of higher order modes on the field homogeneity. The results demonstrate the superimposition of a tolerable standing wave, due to reflections at the cell terminations, and a slight beat wave due to the interference between different modes. Nevertheless, the final outcome is that the system can still be efficiently used to expose small animals in specific WiFi channels, provided they are properly placed in correspondence to the maxima of the electric field along the guide length.

关键词： in vivo Experiments Exposure System TEM Cell WiFi Signal

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Unbiased Estimations of Atmosphere Vortices: The Saturn’s Storm by Cassini VIMS-V as Case Study

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Journal of Signal and Information Processing 2016年第2期7卷 75-83页

作者： Maria L. Moriconi A. Adriani E. D’Aversa G. L. liberti G. Filacchione F. Oliva Institute of Atmospheric and Climatic Sciences-CNR Roma Italy Institute of Astrophysics and Space Planetology-INAF Roma Italy

The size determination of dynamical structures from spectral images poses the question where to fix the shape’s boundary. Here, we propose a method, suitable for nearly elliptical shape, based on the fit of a 2D Gaussian to the pixel intensities of the spectral image. This method has been tested on a vortex structure embedded in the wake of the 2010 Saturn’s giant storm. On January 4th 2012, the Visual and Infrared Mapping Spectrometer (VIMS), onboard Cassini, observed a giant vortex in the Saturn’s northern hemisphere. The structure was embedded in the wake storm system detected on December 2010 by Fletcher et al. [1]. Therefore, all the VIMS observations focused on the Saturn’s storm have been analyzed to investigate its morphology and development. VIMS detected the vortex from May 2011up to January 2012. The evolution of shape and size has been determined for the vortex cloud top, visible at 890 nm. The largest size resulted 4000 km about and seemed to shrinks continuously up to January 2012, while the shape varied in the second half of the year. The vortex oscillated in 2 degrees latitude around 37°N planetocentric latitude, and drifted in longitude by ~0.75 deg/day in westward direction.

关键词： Remote Sensing Geophysical Signal Processing Image Processing and Understanding

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青春期人类免疫缺陷病毒患者窗口期经输血传播给两例受血者

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国外医学（输血及血液学分册） 2005年第5期28卷 465-466页

作者： Phelps R Robbins K liberti T 杨晓芬武江河北省血液中心河北省医科大学第二附属医院

美国在对献血者进行病毒核酸检测(NAT)前,估计重复献血者通过输血传播艾滋病毒(HIV)的几率为1/4.5万～1/6.6万.采用NAT后,风险估计为1/213.5万;初次献血者传播HIV的风险为1/120万到1/180万.从1999年3月起,献血者除按标准检测HIV-1/2EIA... 详细信息

美国在对献血者进行病毒核酸检测(NAT)前,估计重复献血者通过输血传播艾滋病毒(HIV)的几率为1/4.5万～1/6.6万.采用NAT后,风险估计为1/213.5万;初次献血者传播HIV的风险为1/120万到1/180万.从1999年3月起,献血者除按标准检测HIV-1/2EIA和P24抗原外,还做NAT检查.NAT进一步缩短了HIV感染后的窗口期.虽然在献血人群中的HIV检测已经有了很大的进步,输血传播HIV的危险已经很小了,但风险终究不是零.对于HIV的传播,公共卫生机构必须进行有效和系统性调查以确定预防失误和紧急的流行趋势,并立即干预.本文报道2例经输血感染HIV,证实是通过输注1名18岁女性献血者的血液后感染的,以及这名女性感染者的情况.

关键词：经输血传播人类免疫缺陷病毒窗口期受血者青春期 HIV-1 初次献血者输血传播HIV 患者

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动态血压再评估可改善非透析慢性肾脏病患者的风险分层

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中华高血压杂志 2015年第7期23卷 699-699页

作者： Minutolo R Gabbai FB Chiodini P Garofalo C Stanzione G liberti ME Pacilio M Borrelli S Provenzano M Conte G Nicola L 李金凤叶鹏

在非透析慢性肾脏病风险预后评估中,动态血压比诊室血压更有效,但是动态血压再评估能否进一步明确预后尚未知。研究人员纳入间隔1年行2次动态血压测量的高血压合并非透析慢性肾脏病患者182例,分析由距离基线测量1年后获取的第2次动态血... 详细信息

在非透析慢性肾脏病风险预后评估中,动态血压比诊室血压更有效,但是动态血压再评估能否进一步明确预后尚未知。研究人员纳入间隔1年行2次动态血压测量的高血压合并非透析慢性肾脏病患者182例,分析由距离基线测量1年后获取的第2次动态血压测定值对肾病复合终点（死亡,慢性透析,估算的肾小球滤过率下降≥40%）的评估是否有帮助。

关键词：动态血压测量慢性肾脏病预后评估非透析风险患者研究人员慢性透析

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