Effects of protein deprivation and re-feeding on P2X_2 receptors in enteric neurons

作     者：Rúbia Misawa Priscila Azevedo Girotti Márcia Sanae Mizuno Edson Aparecido Liberti John Barton Furness Patricia Castelucci 

作者机构：Department of AnatomyInstitute of Biomedical SciencesUniversity of So Paulo Department of Anatomy and Cell BiologyUniversity of Melbourne 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2010年第16卷第29期

页      面：3651-3663页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

基　　金：Supported by Fundao de Amparo à Pesquisa do Estado de So Paulo/Proc 04/00746-3 

主　　题：Chemical coding Myenteric neurons Submucosal neurons Undernutrition 

摘      要：AIM:To investigate the effects of malnutrition and refeeding on the P2X2 receptor,nitric oxide synthase(NOS),calretinin,calbindin and choline acetyltransferase(ChAT) in neurons of the rat ***:We analyzed the co-localization,numbers and sizes of P2X2-expressing neurons in relation to NOS-immunoreactive(IR),calbindin-IR,ChAT-IR,and calretinin-IR neurons of the myenteric and submucosal *** experimental groups consisted of:(1) rats maintained on normal feed throughout pregnancy until 42 d post-parturition(N);(2) rats deprived of protein throughout pregnancy and 42 d post-parturition(D);and(3) rats undernourished for 21 d post-parturition and then given a protein diet from days 22 to 42(DR).The myenteric and submucosal plexuses were evaluated by double labeling by immunohistochemical methods for P2X2 receptor,NOS,ChAT,calbindin and ***:We found similar P2X2 receptor immunoreactivity in the cytoplasm and surface membranes of myenteric and submucosal neurons from the N,D and DR *** labeling of the myenteric plexus demonstrated that approximately 100% of NOS-IR,calbindin-IR,calretinin-IR and ChAT-IR neurons in all groups also expressed the P2X2 *** the submucosal plexus,the calretinin-IR,ChAT-IR and calbindin-IR neurons were nearly all immunoreactive for the P2X2 *** the myenteric plexus,there was a 19% increase in numbers per cm2 for P2X2 receptor-IR neurons,64% for NOS-IR,84% for calretinin-IR and 26% for ChAT-IR neurons in the D *** spatial density of calbindin-IR neurons,however,did not differ among the three *** submucosal neuronal density increased for calbindin-IR,calretinin-IR and ChAT-IR *** average size of neurons in the myenteric plexus neurons in the D group was less than that in the controls and,in the re-fed rats;there was a 34% reduction in size only for the calretinin-IR ***:This work demonstrates that expression of the P2X2 receptor is present in inhibitory,intrins