Miniscope3D:optimized single-shot miniature 3D fluorescence microscopy
作者机构：UCB/UCSF Joint Graduate Program in BioengineeringUniversity of CaliforniaBerkeleyCA 94720USA Department of Electrical Engineering&Computer SciencesUniversity of CaliforniaBerkeleyCA 94720USA TIPs DepartmentUniversite libre de Bruxelles(ULB)1050 BrusselsBelgium
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2020年第9卷第1期
页 面：367-379页
核心收录：
学科分类：0808[工学-电气工程] 070207[理学-光学] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0803[工学-光学工程] 0702[理学-物理学]
基 金：supported in part by the Defense Advanced Research Projects Agency(DARPA),contract no.N66001-17-C-4015 Gordon and Betty Moore Foundation Data-Driven Discovery Initiative(grant GBMF4562) National Institutes of Health(NIH)grant 1R21EY027597-01 the National Science Foundation(grant no.1617794) an Alfred P.Sloan Foundation fellowship funding from the National Science Foundation Graduate Research Fellowship Program(NSF GRFP)
主 题：enable resolution narrow
摘 要：Miniature fluorescence microscopes are a standard tool in systems ***,widefield miniature microscopes capture only 2D information,and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth ***,we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an optimized multifocal phase mask at the objective’s aperture *** the phase mask at the aperture stop significantly reduces the size of the device,and varying the focal lengths enables a uniform resolution across a wide depth *** phase mask encodes the 3D fluorescence intensity into a single 2D measurement,and the 3D volume is recovered by solving a sparsity-constrained inverse *** provide methods for designing and fabricating the phase mask and an efficient forward model that accounts for the fieldvarying aberrations in miniature *** demonstrate a prototype that is 17mm tall and weighs 2.5 grams,achieving 2.76μm lateral,and 15μm axial resolution across most of the 900×700×390μm^(3) volume at 40 volumes per *** performance is validated experimentally on resolution targets,dynamic biological samples,and mouse brain *** with existing miniature single-shot volume-capture implementations,our system is smaller and lighter and achieves a more than 2×better lateral and axial resolution throughout a 10×larger usable depth *** microscope design provides single-shot 3D imaging for applications where a compact platform matters,such as volumetric neural imaging in freely moving animals and 3D motion studies of dynamic samples in incubators and lab-on-a-chip devices.
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