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PIF4 interacts with ABI4 to serve as a transcriptional activator complex to promote seed dormancy by enhancing ABA biosynthesis and signaling

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Journal of Integrative Plant Biology 2024年第5期66卷 909-927页

作者： Xiaofeng Luo Yujia Dai Baoshan Xian Jiahui Xu Ranran Zhang Muhammad Saad Rehmani Chuan Zheng Xiaoting Zhao Kaitao Mao Xiaotong Ren Shaowei Wei Lei Wang Juan He weiming tan Junbo Du Weiguo Liu Shu Yuan Kai Shu Shaanxi Key Laboratory of Qinling Ecological Intelligent Monitoring and Protection School of Ecology and EnvironmentNorthwestern Polytechnical UniversityXi'an 710129China Research&Development Institute of Northwestern Polytechnical University in ShenzhenShenzhen 518057China College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China Institute of Ecological Agriculture Sichuan Agricultural UniversityChengdu 611130China College of Resources Sichuan Agricultural UniversityChengdu 611130China

Transcriptional regulation plays a key role in the control of seed dormancy,and many transcription factors(TFs)have been ***,the mechanisms underlying the interactions between different TFs within a transcriptional complex regulating seed dormancy remain largely ***,we showed that TF PHYTOCHROME-INTERACTING FACTOR4(PIF4)physically interacted with the abscisic acid(ABA)signaling responsive TF ABSCISIC ACID INSENSITIVE4(ABI4)to act as a transcriptional complex to promote ABA biosynthesis and signaling,finally deepening primary seed *** pif4 and abi4 single mutants exhibited a decreased primary seed dormancy phenotype,with a synergistic effect in the pif4/abi4 double ***4 binds to ABI4 to form a heterodimer,and ABI4 stabilizes PIF4 at the protein level,whereas PIF4 does not affect the protein stabilization of ***,both TFs independently and synergistically promoted the expression of ABI4 and NCED6,a key gene for ABA *** genetic evidence is also consistent with the phenotypic,physiological and biochemical analysis ***,this study revealed a transcriptional regulatory cascade in which the PIF4–ABI4 transcriptional activator complex synergistically enhanced seed dormancy by facilitating ABA biosynthesis and signaling.

关键词： ABA ABI4 PIF4 seed dormancy and germination transcriptional complex

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现代化进程中益阳市农业产业体系构建研究

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农业技术与装备 2024年第9期 83-85页

作者：谭伟明曹佩蒋上志陈苏敏益阳职业技术学院湖南益阳413000 长沙工业学院湖南长沙410200

在介绍益阳市现代农业产业体系发展现状的基础上,分析了益阳市现代农业产业体系发展中存在的问题,从谋规划、拓渠道、调结构、强体系等方面,探索了构建益阳现代农业产业技术体系建设新路径。

关键词：现代化进程农业产业体系构建研究品牌打造益阳市

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Monoclonal Antibody-Based Enzyme Linked Immunosorbent Assay for the Analysis of Jasmonates in Plants

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Journal of Integrative Plant Biology 2008年第8期50卷 1046-1052页

作者： Aixing Deng weiming tan Suping He Wei Liu Tiegui Nan Zhaohu Li Baomin Wang Qing X. Li College of Agronomy and Biotechnology China Agricultural University Beijing 100094 China Department of Molecular Biosciences and Bioengineering University of Hawaii Honolulu Hawaii 96822 USA

Methyl jasmonate （MeJA） and its free-acid form, jasmonic acid （JA） are naturally occurring plant growth regulators widely distributed in higher plants. In order to improve the sensitivity for the analysis of MeJA at low levels in small amounts of plant samples, a monoclonal antibody （MAb）（designated as MAb 3E5D7C4B6） against MeJA was derived from a JA- bovine serum albumin （BSA） conjugate as an immunogen. The antibody belongs to the IgG1 subclass with a κ type light chain and has a dissociation constant of approximately 6.07 ×10^-9 M. MAb3E5D7C4B6 is very specific to MeJA. It was used to develop a direct competitive enzyme-linked immunosorbent assay （dcELISA）, conventional and simplified indirect competitive ELISAs （icELISA）. JA was derivatized into MeJA for the ELISA analysis. The IC50 value and detection range for MeJA were, respectively, 34 and 4-257 nglmL by the conventional icELISA, 21 and 3-226 nglmL by the simplified icELISA and 5.0 and 0.7-97.0 nglmL by the dcELISA. The dcELISA was more sensitive than either the conventional or simplified icELISA. The assays were used to measure the content of jasmonates as MeJA in tobacco leaves under drought stress or inoculated with tobacco mosaic virus and tomato leaves inoculated with tomato mosaic virus or Lirioinyza sativae Blanchard as compared with the corresponding healthy leaves. The increased jasmonates content indicated its role in response to the drought stress and pathogens.

关键词： enzyme-linked immunosorbent assay immunoassay methyl jasmonate plant growth regulators

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Analysis of trace elements in air particulate matters by non-suppressed ion chromatography

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Journal of Environmental Sciences 1991年第1期3卷 29-39页

作者： Feng Yingsheng(Y.S.Fung) and tan weiming (W.M.Tam)Department of Chemistry,Hong Kong University,Hong Kong

The application of non-suppressed ion chromatography for monitoring of trace elements in air particulate matter was studied in the present investigation. The results indicate that the use of microwave acid digestion method is superior in comparison with the conventional thermal acid digestion method as it leads to higher recovery, better reproducibility, lower volatility loss, better protection against environmental contamination and much less digestion time (5 minutes vs. 24 hours). The use of eluent as extractant is shown to reduce the water dip problem in the chro-matogram. The addition of chelating agent in the eluent coupled with UV detection is shown to provide satisfactory chromatographic separation and good sensitivity for the analysis of transition metals present in the air particulate matter. Using the U.S. National Bureau of Standards Reference Material 1648 Urban Particulate Matter as standard for checking, the analytical procedure is shown to give good recovery and reproducibility for the detection of the following cations and anions in air particulate matter: Fe2 Cu, Mn, Pb, Zn, Mg, Na, HN4+, Cl-, NO3- and SO42-. Field test was also performed to check the applicability of the method and the results obtained were discussed in the present paper.

关键词： non-suppressed ion chromatography air particulate matter trace metals analysis anions analysis microwave digestion.

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Dynamical pattern recognition for univariate time series and its application to an axial compressor

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Control Theory and Technology 2024年第1期22卷 39-55页

作者： Jingtao Hu weiming Wu Zejian Zhu Cong Wang Center for Intelligent Medical Engineering School of Control Science and TechnologyShandong UniversityJinan250061ShandongChina School of Automation Science and Engineering South China University of TechnologyGuangzhou510641GuangdongChina

In this paper, a learning and recognition approach is proposed for univariate time series composed of output measurements of general nonlinear dynamical systems. Firstly, a class of dynamical systems in the canonical form is derived to describe the univariate time series by introducing coordinate transformation. An observer-based deterministic learning technique is then adopted to achieve dynamical modeling of the associated transformed systems of the training univariate time series, and the modeling results in the form of radial basis function network (RBFN) models are stored in a pattern library. Subsequently, multiple observer-based dynamical estimators containing the RBFN models in the pattern library are constructed for a test univariate time series, and a recognition decision scheme is proposed by the derived recognition indicator. On this basis, more concise recognition conditions are provided, which is beneficial for verifying the recognition results. Finally, simulation studies on the Rossler system and aero-engine stall warning verify the effectiveness of the proposed approach.

关键词： Dynamical pattern recognition Deterministic learning Stall warning Radial basis function network Sampled-data observer

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Joint Modeling of Citation Networks and User Preferences for Academic Tagging Recommender System

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Computers, Materials & Continua 2024年第6期79卷 4449-4469页

作者： weiming Huang Baisong Liu Zhaoliang Wang Faculty of Electrical Engineering and Computer Science Ningbo UniversityNingbo315211China Inner Mongolia Metal Material Research Institute Baotou014000China

In the tag recommendation task on academic platforms,existing methods disregard users’customized preferences in favor of extracting tags based just on the content of the ***,it uses co-occurrence techniques and tries to combine nodes’textual content for *** still do not,however,directly simulate many interactions in network *** order to address these issues,we present a novel system that more thoroughly integrates user preferences and citation networks into article labelling ***,we first employ path similarity to quantify the degree of similarity between user labelling preferences and articles in the citation ***,the Commuting Matrix for massive node pair paths is used to improve computational ***,the two commonalities mentioned above are combined with the interaction paper labels based on the additivity of Poisson *** addition,we also consider solving the model’s parameters by applying variational *** results demonstrate that our suggested framework agrees and significantly outperforms the state-of-the-art baseline on two real datasets by efficiently merging the three relational *** on the Area Under Curve(AUC)and Mean Average Precision(MAP)analysis,the performance of the suggested task is evaluated,and it is demonstrated to have a greater solving efficiency than current techniques.

关键词： Collaborative filtering citation networks variational inference poisson factorization tag recommendation

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齿轮副端面重合度统一定义及其计算式

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机械传动 2005年第6期29卷 40-42页

作者：谭伟明佛山科学技术学院机电工程系广东佛山528000

针对各种齿轮传动提出端面重合度的统一定义,并且推导其计算表达式,进一步讨论了渐开线齿轮副、微线段齿轮副和正弦齿廓齿轮副的端面重合度的计算问题。齿轮副的端面重合度定义为齿轮作用角(即一对轮齿从进入啮合到脱离啮合过程中齿轮... 详细信息

针对各种齿轮传动提出端面重合度的统一定义,并且推导其计算表达式,进一步讨论了渐开线齿轮副、微线段齿轮副和正弦齿廓齿轮副的端面重合度的计算问题。齿轮副的端面重合度定义为齿轮作用角(即一对轮齿从进入啮合到脱离啮合过程中齿轮所转过的角度)与齿轮的齿距转角的比值。根据齿轮啮合原理,由基本齿条的轮廓曲线能够获得其啮合线方程。根据所获得的啮合线方程,以及给定的齿轮副齿顶线方程,就能够根据本文的计算式得到齿轮副的重合度。对于渐开线齿轮副,该定义与众所周知的“啮合线长度与基节之比”的结果相同。该定义同样适用于非渐开线齿轮副,例如微线段齿轮副、正弦齿廓齿轮副等,而且计算结果更可靠。

关键词：齿轮副重合度作用角齿距转角微线段齿轮正弦齿廓齿轮

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混合驱动的滚齿机传动系统设计

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机械传动 2016年第4期40卷 87-90,100页

作者：谭伟明佛山科学技术学院机电工程系广东佛山528000

提出了一种基于混合驱动的内联系传动链作为滚齿机的范成传动链,意在同时解决滚齿机传动系统设计中的范成和差动这两个主要问题。利用一个自由度为2的差动齿轮机构作为运动合成机构,将一个机械传动运动和一个电子传动运动相叠加,实现粗... 详细信息

提出了一种基于混合驱动的内联系传动链作为滚齿机的范成传动链,意在同时解决滚齿机传动系统设计中的范成和差动这两个主要问题。利用一个自由度为2的差动齿轮机构作为运动合成机构,将一个机械传动运动和一个电子传动运动相叠加,实现粗略调整和精细调整的结合。以混合驱动为基础,比较细致地进行了加工齿数为11~110范围的滚齿机传动系统的设计。传动系统设计结果表明,这种方法能够满足滚齿机的范成运动的要求。

关键词：滚齿机传动系统内联系传动链混合驱动

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混合驱动内联系传动链的建模及仿真

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机械传动 2015年第11期39卷 76-79页

作者：谭伟明佛山科学技术学院机电工程系广东佛山528000

建立基于混合驱动的内联系传动链动态模型,并进行数字仿真。利用伺服电机作为执行机构对于传动链的可调整部分施加补偿运动,从而使得末端件的实际速度接近其理想速度。建立传动链的动态模型后,利用MATLAB的Simulink工具进行了计算机建... 详细信息

建立基于混合驱动的内联系传动链动态模型,并进行数字仿真。利用伺服电机作为执行机构对于传动链的可调整部分施加补偿运动,从而使得末端件的实际速度接近其理想速度。建立传动链的动态模型后,利用MATLAB的Simulink工具进行了计算机建模及仿真。以滚齿机主传动系统作为仿真对象,对齿数为38、50的齿轮切削过程的速度变化情况进行数字仿真。结果证实了这种混合驱动内联系传动链是可行的,其传动误差与末端件的运动检测传感器误差是等量值的。

关键词：内联系传动链混合驱动建模仿真 MATLAB

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混合驱动内联系传动链中的运动合成机构分析

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机械传动 2015年第4期39卷 110-114页

作者：谭伟明佛山科学技术学院机电工程系广东佛山528000

讨论了基于混合驱动的内联系传动链中可能应用的几种运动合成机构。二自由度周转齿轮系有两个输入运动和一个输出运动,故适合作为这种合成机构。其中2K-H型结构比较简单,是常用的二自由度周转齿轮系,本文讨论了其中3种类型的力矩平衡和... 详细信息

讨论了基于混合驱动的内联系传动链中可能应用的几种运动合成机构。二自由度周转齿轮系有两个输入运动和一个输出运动,故适合作为这种合成机构。其中2K-H型结构比较简单,是常用的二自由度周转齿轮系,本文讨论了其中3种类型的力矩平衡和功率平衡特征。获得结论为:2KH型的NGW负号机构能够满足运动合成机构的性能要求;2K-H型的WW正号机构在一定条件下能够满足性能要求;而滚齿机上常用的差速器机构通常不满足这种性能要求。

关键词：内联系传动链混合驱动运动合成机构周转齿轮系 NGW机构 WW机构差速器机构

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