PIF4 interacts with ABI4 to serve as a transcriptional activator complex to promote seed dormancy by enhancing ABA biosynthesis and signaling

作     者：Xiaofeng Luo Yujia Dai Baoshan Xian Jiahui Xu Ranran Zhang Muhammad Saad Rehmani Chuan Zheng Xiaoting Zhao Kaitao Mao Xiaotong Ren Shaowei Wei Lei Wang Juan He Weiming Tan Junbo Du Weiguo Liu Shu Yuan Kai Shu 

作者机构：Shaanxi Key Laboratory of Qinling Ecological Intelligent Monitoring and ProtectionSchool of Ecology and EnvironmentNorthwestern Polytechnical UniversityXi'an 710129China Research&DevelopmentInstitute of Northwestern Polytechnical University in ShenzhenShenzhen 518057China College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Institute of Ecological AgricultureSichuan Agricultural UniversityChengdu 611130China College of ResourcesSichuan Agricultural UniversityChengdu 611130China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2024年第66卷第5期

页      面：909-927页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the National Natural Science Foundation of China(31872804 and 32101670) Natural Science Basic Research Program of Shaanxi(2024JC-YBMS-151) Shaanxi Fundamental Science Research Project for Chemistry&Biology(22JHQ054 and 22JHZ007) GuangDong Basic and Applied Basic Research Foundation(2021A1515110341) the Innovation Foundation for Doctoral Dissertations of Northwestern Polytechnical University(CX2021040,CX2022079 and CX2023096) Postdoctoral Research Foundation of China(2021M692644,2021M702674) 

主　　题：ABA ABI4 PIF4 seed dormancy and germination transcriptional complex 

摘      要：Transcriptional regulation plays a key role in the control of seed dormancy,and many transcription factors(TFs)have been ***,the mechanisms underlying the interactions between different TFs within a transcriptional complex regulating seed dormancy remain largely ***,we showed that TF PHYTOCHROME-INTERACTING FACTOR4(PIF4)physically interacted with the abscisic acid(ABA)signaling responsive TF ABSCISIC ACID INSENSITIVE4(ABI4)to act as a transcriptional complex to promote ABA biosynthesis and signaling,finally deepening primary seed *** pif4 and abi4 single mutants exhibited a decreased primary seed dormancy phenotype,with a synergistic effect in the pif4/abi4 double ***4 binds to ABI4 to form a heterodimer,and ABI4 stabilizes PIF4 at the protein level,whereas PIF4 does not affect the protein stabilization of ***,both TFs independently and synergistically promoted the expression of ABI4 and NCED6,a key gene for ABA *** genetic evidence is also consistent with the phenotypic,physiological and biochemical analysis ***,this study revealed a transcriptional regulatory cascade in which the PIF4–ABI4 transcriptional activator complex synergistically enhanced seed dormancy by facilitating ABA biosynthesis and signaling.