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MicroRNA profile in neosquamous esophageal mucosa following ablation of Barrett's esophagus

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World journal of Gastroenterology 2017年第30期23卷 5508-5518页

作者： Loveena Sreedharan George C Mayne David I watson Timothy Bright Reginald V Lord Alfiya Ansar Tingting Wang jakob Kist David Stj Astill Damian j Hussey Department of Surgery Flinders Medical Centre Flinders University St Vincent’s Centre for Applied Medical Research Department of Anatomical Pathology Flinders Medical Centre

To investigate the microRNA expression profile in esophageal neosquamous epithelium from patients who had undergone ablation of Barrett’s esophagus. METHODSHigh throughput screening using TaqMan® Array Human MicroRNA quantitative PCR was used to determine expression levels of 754 microRNAs in distal esophageal mucosa (1 cm above the gastro-esophageal junction) from 16 patients who had undergone ablation of non-dysplastic Barrett’s esophagus using argon plasma coagulation vs pretreatment mucosa, post-treatment proximal normal non-treated esophageal mucosa, and esophageal mucosal biopsies from 10 controls without Barrett’s esophagus. Biopsies of squamous mucosa were also taken from 5 cm above the pre-ablation squamo-columnar junction. Predicted mRNA target pathway analysis was used to investigate the functional involvement of differentially expressed microRNAs. RESULTSForty-four microRNAs were differentially expressed between control squamous mucosa vs post-ablation neosquamous mucosa. Nineteen microRNAs were differentially expressed between post-ablation neosquamous and post-ablation squamous mucosa obtained from the more proximal non-treated esophageal segment. Twelve microRNAs were differentially expressed in both neosquamous vs matched proximal squamous mucosa and neosquamous vs squamous mucosa from healthy patients. Nine microRNAs (miR-424-5p, miR-127-3p, miR-98-5p, miR-187-3p, miR-495-3p, miR-34c-5p, miR-223-5p, miR-539-5p, miR-376a-3p, miR-409-3p) were expressed at higher levels in post-ablation neosquamous mucosa than in matched proximal squamous and healthy squamous mucosa. These microRNAs were also more highly expressed in Barrett’s esophagus mucosa than matched proximal squamous and squamous mucosa from controls. Target prediction and pathway analysis suggests that these microRNAs may be involved in the regulation of cell survival signalling pathways. Three microRNAs (miR-187-3p, miR-135b-5p and miR-31-5p) were expressed at higher levels in post-

关键词： Neosquamous Barrett’s esophagus Ablation

Lifetime risk of esophageal adenocarcinoma in patients with Barrett's esophagus

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World journal of Gastroenterology 2014年第28期20卷 9611-9617页

作者： Piers Gatenby Christine Caygill Christine Wall Santanu Bhatacharjee james Ramus Anthony watson Marc Winslet Division of Surgery and Interventional Science UCLLondonNW32QGUnited Kingdom

AIM:To investigate the lifetime risk of development of esophageal adenocarcinoma and/or high-grade dysplasia in patients diagnosed with Barrett’s ***:Data were extracted from the United Kingdom National Barrett’s Oesophagus Registry on date of diagnosis,patient age and gender of 7877 patients from who had been registered from 35 United Kingdom *** expectancy was evaluated from United Kingdom National Statistics data based upon gender and age at year at *** data were then used with published estimates of annual adenocarcinoma and high-grade dysplasia incidences from metaanalyses and large population-based studies to estimate overall lifetime risk of development of these study ***:The mean age at diagnosis of Barrett’s esophagus was 61.6 years in males and 67.3 years in *** mean life expectancy at diagnosis was23.1 years in males,20.7 years in females and 22.2years *** data from published meta-analyses,the lifetime risk of development of adenocarcinoma was between 1 in 8 and 1 in 14 and the lifetime risk of high-grade dysplasia or adenocarcinoma was 1 in 5 to1 in *** data from 3 large recent population-based cohort studies the lifetime risk of adenocarcinoma was between 1 in 10 and 1 in 37 and of the combined endpoint of high-grade dysplasia and adenocarcinoma was between 1 in 8 and 1 in *** at Barrett’s esophagus diagnosis is reducing and life expectancy is increasing,which will partially counter-balance lower annual cancer ***:There is a significant lifetime risk of development of high-grade dysplasia and adenocarcinoma in Barrett’s esophagus.

关键词： Barrett esophagus Esophageal neoplasms Forecasting Meta-analysis Cohort studies

围孕期补充叶酸和／或多种维生素预防神经管缺陷

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生殖与避孕 2005年第7期25卷 430-434页

作者： Lumnley j watson L watson M Bower C 陈晓军丰有吉单位不详

1背景神经管缺陷(NTDs)是在脑和脊索发育过程中形成的先天性畸形,包括无脑儿(颅骨、表面皮肤和脑组织全部或部分缺失)、脊柱裂(脊柱未闭合造成的脊索、脊膜分别或同时突出或暴露;有些病例合并脑积水)和脑膨出(脑膜疝,脑组织位于颅骨外,... 详细信息

1背景神经管缺陷(NTDs)是在脑和脊索发育过程中形成的先天性畸形,包括无脑儿(颅骨、表面皮肤和脑组织全部或部分缺失)、脊柱裂(脊柱未闭合造成的脊索、脊膜分别或同时突出或暴露;有些病例合并脑积水)和脑膨出(脑膜疝,脑组织位于颅骨外,表面被正常或萎缩皮肤覆盖).

关键词：神经管缺陷多种维生素神经管闭合不全预防叶酸孕期皮肤覆盖先天性畸形无脑儿脊柱裂

Clinical supervision for novice millennial nurses in the perinatal setting: The need for generational sensitivity

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Open journal of Nursing 2013年第5期3卷 373-378页

作者： jo watson G. j. Macdonald Donna Brown Lawrence S Bloomberg Faculty of Nursing University of Toronto Toronto Canada Sunnybrook Health Sciences Centre Toronto Canada

This paper reports on a qualitative research study that examined the experience of expert and novice nurses participating in a new, reflective program of “clinical supervision”, intending to facilitate the transition of new graduate nurses into the workforce. Three patterns emerged during the constructivist inquiry: readiness to reflect, valuing of clinical supervision, and sustainability of the clinical supervision model. The researchers suggest generational sensitivity as a key perspective to consider when developing engaging workplace strategies for millennial nurses. The article offers recommendations for the implementation of clinical supervision and would be of interest to nurse leaders in a clinical setting.

关键词： New Graduate Nurses Clinical Supervision Generational Sensitivity Constructivist Inquiry

Chronic exposure to high-density polyethylene microplastic through feeding alters the nutrient metabolism of juvenile yellow perch (Perca flavescens)

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Animal Nutrition 2022年第2期9卷 143-158页

作者： Xing Lu Dong-Fang Deng Fei Huang Fabio Casu Emma Kraco Ryan j.Newton Merry Zohn Swee j.Teh Aaron M.watson Brian Shepherd Ying Ma Mahmound A.O.Dawood Lorena M.Rios Mendoza School of Freshwater Sciences University of WisconsinMilwaukeeWI 53204USA South Carolina Department of Natural Resources CharlestonSC 29412USA USDA/ARS/School of Freshwater Sciences University of WisconsinMilwaukeeWI 53204USA School of Veterinary Medicine Department of AnatomyPhysiologyand Cell BiologyUniversity of CaliforniaDavisCA 95616USA Department of Natural Sciences Marine Resources Research InstituteUniversity of WisconsinSuperiorWI 54880USA Yangtze River Fisheries Research Institute Chinese Academy of Fishery SciencesWuhanChina Fisheries College Jimei UniversityXiamenChina. Faculty of Agriculture Kafrelsheikh UniversityKafr El-SheikhEgypt.

Microplastics are emergent contaminants threatening aquatic organisms including aquacultured *** study investigated the effects of high-density polyethylene(HDPE,100 to 125 mm)on yellow perch(Perca flavescens)based on integrative evaluation including growth performance,nutritional status,nutrient metabolism,fish health,and gut microbial *** test diets(0,1,2,4,or 8 g HDPE/100 g diet)containing 41%protein and 10.5%lipid were fed to juvenile perch(average body weight,25.9±0.2 g;n=15)at a feeding rate of 1.5%to 2.0%body weight *** feeding trial was conducted in a flow-through water system for 9 wk with 3 tanks per treatment and 15 yellow perch per *** mortality or HDPE accumulation in the fish was found in any *** gain and condition factor of fish were not significantly impacted by HDPE(P>0.05).Compared to the control group,fish fed the 8%HDPE diet had significantly decreased levels of protein and ash(P<0.05).In response to the increasing levels of HDPE exposure,the hepatosomatic index value,hepatocyte size,and liver glycogen level were increased,but lipid content was reduced in the liver *** to the control treatment,fish fed the 8%HDPE diet had significant accumulations of total bile acids and different metabolism pathways such as bile acid biosynthesis,pyruvate metabolism,and carnitine *** enterocyte necrosis was documented in the foregut of fish fed the 2%or 8%HDPE diet;and significant cell sloughing was observed in the midgut and hindgut of fish fed the 8%HDPE *** fed the 2%HDPE diet harbored different microbiota communities compared to the control *** study demonstrates that HDPE ranging from 100 to 125 mm in feed can be evacuated by yellow perch with no impact on ***,dietary exposure to HDPE decreased whole fish nutrition quality,altered nutrient metabolism and the intestinal histopathology as well as microbiota community of yellow *** results indicate that extende

关键词： Dietary exposure Intestinal microbiota Liver metabolomics Microplastics Nutrient composition Yellow perch

Real-world emission factors for Caterpillar 797B heavy haulers during mining operations

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Particuology 2016年第5期14卷 22-30页

作者： Xiaoliang Wang judith C. Chow Steven D. Kohl Kevin E. Percy Allan H. Legge john G. watson Desert Research Institute 2215 Raggio Parkway Reno NV 895J2 USA Graduate Faculty University of Nevada Reno NV89503 USA The State Key Laboratory of Loess and Quaternary Geology Institute of Earth Environment Chinese Academy of Sciences Xi'an 710075 China Wood Buffalo Environmental Association 330 Thickwood Boulevard Fort McMurray AB Canada 7DK 1 Y1 Biosphere Solutions 1610 1 lth Avenue N.W. Calgary AB Canada T2N IG9

Real-world fuel-based emission factors （EFs） from Caterpillar 797B diesel heavy haulers when used for oil sands mining operations were measured using an on-hoard portable emissions monitoring system. Average EFs （in g/kg fuel） for 16 separate tests on four trucks were 3150 ± 4 for CO2, 2.51 ± 1.30 for CH4, 10.28 ± 3.21 for CO, 0.61 ± 0.32 for non-methane hydrocarbons, 55.49 ± 9,75 for NOx （reported as NO2）, 0.70 ± 0.17 for PM2.s （mass of particles with aerodynamic diameter 〈2.5 μm）, and 0.34 ± 0.05 for black carbon （BC）. Ultrafine particle numbers averaged （4.7 ± 4.1 ） × 10^15 particleslkg fuel. Fuel-based EFs of NO, NO2, and particle numbers were higher during idling, whereas PM2.5 and BC EFs were higher during traveling without a load. A comparison of emissions with those reported by oil sands facilities showed large variations, both among the facilities and between the reported emissions and the real-world measurements.

关键词： Heavy hauler Emission Nonroad Portable emissions measurement system Mining Oil sands

用光调制反射研究GaSb/AlSb多层量子势阱的带间跃迁

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镇江船舶学院学报 1989年第2期3卷 38-44页

作者： H.Shen Z.Hang 冷静 F.H.Pollak L.L.Chang W.I.Wang L.Esaki 美国纽约城市大学布鲁克林学院物理系美国IBM公司

我们测量了两个GaSb/AlSb多层量子势阱在77K时的光调制反射谱。在Г点（GaSb）亚带间的能量,理论与实验符合得甚好,这种一致性可延伸到空穴态被束缚而电子态低于AlSb导带最小值X点的整个能量范围, 这就使得有可能确定导带的补偿参数0.85... 详细信息

我们测量了两个GaSb/AlSb多层量子势阱在77K时的光调制反射谱。在Г点（GaSb）亚带间的能量,理论与实验符合得甚好,这种一致性可延伸到空穴态被束缚而电子态低于AlSb导带最小值X点的整个能量范围, 这就使得有可能确定导带的补偿参数0.85±0.08（应变前）和应变的贡献,超过这个能量范围时,理论与实验的偏离则为Г-X混合态提供了证据。

关键词： GaSb/AlSb 量子势阱带间跃迁

Long-term opioid therapy and functional status

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中华物理医学与康复杂志 2017年第10期39卷 789-789页

作者： Hoffman EM watson jC St Sauver j

BACKGROUND AND OBjECTIVE While opioids appeared to be effective for the treatment of non-cancer pain in short durations,the long-term efficacy of these medications is not well *** a longitudinal cohort format,this study followed patients with polyneuropathy,in order to examine the relationship between the duration of opioid therapy and functional status.

关键词：药物治疗状态治疗时间神经病多发性

Serum outperforms plasma in small extracellular vesicle microRNA biomarker studies of adenocarcinoma of the esophagus

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World journal of Gastroenterology 2020年第20期26卷 2570-2583页

作者： Karen Chiam George C Mayne Tingting Wang David I watson Tanya S Irvine Tim Bright Lorelle T Smith Imogen A Ball joanne M Bowen Dorothy M Keefe Sarah K Thompson Damian j Hussey Discipline of Surgery College of Medicine and Public HealthFlinders University of South AustraliaAdelaideSA 5042Australia Flinders Health and Medical Research Institute Cancer Program College of Medicine and Public HealthFlinders UniversityAdelaideSA 5042Australia Discipline of Surgery The University of AdelaideAdelaideSA 5005Australia Adelaide Medical School The University of AdelaideAdelaideSA 5005Australia Faculty of Health Sciences The University of AdelaideAdelaideSA 5005Australia

BACKGROUND Circulating microRNAs(miRNAs)are potential biomarkers for many ***,they can originate from non-disease specific sources,such as blood cells,and compromise the investigations for miRNA *** small extracellular vesicles(sEVs)have been suggested to provide a purer source of circulating miRNAs for biomarkers discovery,the most suitable blood sample for sEV miRNA biomarker studies has not been *** To compare the mi RNA profiles between matched serum and plasma s EV preparations to determine their suitability for biomarker *** Matched serum and plasma samples were obtained from 10 healthy controls and10 patients with esophageal adenocarcinoma.s EV isolates were prepared from serum and plasma using Exo Quick TM and quantified using Nano *** was extracted from s EV preparations with the mi RNeasy Serum/Plasma kit and profiled using the Taqman Openarray q *** overall mi RNA content and theexpression of specific mi RNAs of reported vesicular and non-vesicular origins were compared between serum and plasma s EV *** diagnostic performance of a previously identified multi-mi RNA biomarker panel for esophageal adenocarcinoma was also *** The overall mi RNA content was higher in plasma s EV preparations(480 mi RNAs)and contained 97.5%of the mi RNAs found in the serum s EV preparations(412 mi RNAs).The expression of commonly expressed mi RNAs was highly correlated(Spearman’s R=0.87,P<0.0001)between the plasma and serum s EV preparations,but was consistently higher in the plasma s EV *** blood-cell mi RNAs(hsa-mi R-223-3 p,hsa-mi R-451 a,mi R-19 b-3 p,hsa-mi R-17-5 p,hsa-mi R-30 b-5 p,hsa-mi R-106 a-5 p,hsa-mi R-150-5 p and hsa-mi R-92 a-3 p)were expressed at 2.7 to 9.6 fold higher levels in the plasma s EV preparations compared to serum s EV preparations(P<0.05).In plasma s EV preparations,the percentage of protein-associated mi RNAs expressed at relatively higher levels(Ct

关键词： Biomarkers Exosomes Extracellular vesicles Circulating microRNA MicroRNAs Plasma Serum Blood cells Real-time polymerase chain reaction Adenocarcinoma of esophagus