Chronic exposure to high-density polyethylene microplastic through feeding alters the nutrient metabolism of juvenile yellow perch (Perca flavescens)

作     者：Xing Lu Dong-Fang Deng Fei Huang Fabio Casu Emma Kraco Ryan J.Newton Merry Zohn Swee J.Teh Aaron M.Watson Brian Shepherd Ying Ma Mahmound A.O.Dawood Lorena M.Rios Mendoza Xing Lu;Dong-Fang Deng;Fei Huang;Fabio Casu;Emma Kraco;Ryan J.Newton;Merry Zohn;Swee J.Teh;Aaron M.Watson;Brian Shepherd;Ying Ma;Mahmound A.O.Dawood;Lorena M.Rios Mendoza

作者机构：School of Freshwater SciencesUniversity of WisconsinMilwaukeeWI 53204USA South Carolina Department of Natural ResourcesCharlestonSC 29412USA USDA/ARS/School of Freshwater SciencesUniversity of WisconsinMilwaukeeWI 53204USA School of Veterinary MedicineDepartment of AnatomyPhysiologyand Cell BiologyUniversity of CaliforniaDavisCA 95616USA Department of Natural SciencesMarine Resources Research InstituteUniversity of WisconsinSuperiorWI 54880USA Yangtze River Fisheries Research InstituteChinese Academy of Fishery SciencesWuhanChina Fisheries CollegeJimei UniversityXiamenChina. Faculty of AgricultureKafrelsheikh UniversityKafr El-SheikhEgypt. 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2022年第9卷第2期

页      面：143-158页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：The current study was partially funded by the University of Wisconsin-Milwaukee(UWM,Project 150-25-3150-343 PRJ93WQ) USDA-ARS in-house project#s 5090-31320-004-00D and 5090-31320-005-00D UW-system water research fellowship and UWM SURF were supported to Emma K.Kraco Naulin Foundation was awarded to Deng's lab to support part of the study Merry Zohn was supported by fellowship with the Oak Ridge Institute for Science Education Xing Lu(201803260002)and Fei Huang(201806330033)were supported by the China Scholarship Council Ying Ma was supported by the Education Department of Fujian Province,China 

主　　题：Dietary exposure Intestinal microbiota Liver metabolomics Microplastics Nutrient composition Yellow perch 

摘      要：Microplastics are emergent contaminants threatening aquatic organisms including aquacultured *** study investigated the effects of high-density polyethylene(HDPE,100 to 125 mm)on yellow perch(Perca flavescens)based on integrative evaluation including growth performance,nutritional status,nutrient metabolism,fish health,and gut microbial *** test diets(0,1,2,4,or 8 g HDPE/100 g diet)containing 41%protein and 10.5%lipid were fed to juvenile perch(average body weight,25.9±0.2 g;n=15)at a feeding rate of 1.5%to 2.0%body weight *** feeding trial was conducted in a flow-through water system for 9 wk with 3 tanks per treatment and 15 yellow perch per *** mortality or HDPE accumulation in the fish was found in any *** gain and condition factor of fish were not significantly impacted by HDPE(P0.05).Compared to the control group,fish fed the 8%HDPE diet had significantly decreased levels of protein and ash(P0.05).In response to the increasing levels of HDPE exposure,the hepatosomatic index value,hepatocyte size,and liver glycogen level were increased,but lipid content was reduced in the liver *** to the control treatment,fish fed the 8%HDPE diet had significant accumulations of total bile acids and different metabolism pathways such as bile acid biosynthesis,pyruvate metabolism,and carnitine *** enterocyte necrosis was documented in the foregut of fish fed the 2%or 8%HDPE diet;and significant cell sloughing was observed in the midgut and hindgut of fish fed the 8%HDPE *** fed the 2%HDPE diet harbored different microbiota communities compared to the control *** study demonstrates that HDPE ranging from 100 to 125 mm in feed can be evacuated by yellow perch with no impact on ***,dietary exposure to HDPE decreased whole fish nutrition quality,altered nutrient metabolism and the intestinal histopathology as well as microbiota community of yellow *** results indicate that extende