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Chinese Journal of Chemistry 2023年第22期41卷 3003-3011页

作者： Lu Zhang Qingfeng Du yicong Luo Yuanlin wang Feng Gao Yong Ye Wanbin Zhang Green Catalysis Center College of ChemistryZhengzhou University100 Kexue RoadZhengzhouHenan 450001China Frontiers Science Center for Transformative Molecules School of Chemistry and Chemical EngineeringShanghai Jiao Tong University 800 Dongchuan RoadShanghai 200240China

Skipped dienes are of great importance but remain challenging to synthesize because of the presence of a sp hybrid carbon between the two ***,we have developed the first nickel-catalyzed regio-,(E)-stereo-and linear-selective ring-opening/cross-coupling reaction of vinylaziridines with organoboronic acids under mild conditions to construct various skipped *** reaction exhibits wide functional-group compatibility,and could be adapted for the introduction of skipped aminodiene functionality into bioactive molecules.

关键词： DFT calculation Multifunctional organoboronic acids Ni-catalyzed Ring-opening Skipped aminodiene

Cell-based biosensors:Towards the development of cellular monitoring

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Chinese Science Bulletin 2002年第22期47卷 1849-1856页

作者： Gaixia Xu yicong Wu Rong Li Ping wang Weiming Yan Xiaoxiang Zheng Zhejiang Univ Dept Biomed Engn Minist Educ Biosensor Natl Special LabKey Lab BME Hangzhou 310027 Peoples R China

Cell-based biosensors (CBBs), a research hot-spot of biosensors, which treat living cells as sensing elements, can detect the functional information of biologically active analytes. They characterize with high sensitivity, excellent selectivity and rapid response, and have been applied in many fields, such as biomedicine, environmental monitoring and pharmaceutical screening. Recently cell-cultured technology, silicon microfabrication technology and genetic technology have promoted exploration of CBBs dramatically. To elucidate the novel research findings and applications of cell- based biosensors, this paper summarizes various research approaches, presents some challenges and proposes the research trends.

关键词： cell-based biosensors functional analysis cellular microenvironment electrophysiological genetically-engineered.

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同步电机水冷却器不停机反冲洗的应用与实效

冶金设备管理与维修 2023年第5期41卷 12-14页

作者：巩达关泽磊徐东升张闯梅兴冬王义聪鞍钢股份能源管理中心发电分厂辽宁鞍山114000

针对高炉电动鼓风机运行期间同步电机水冷却器内部由于泥沙污垢等使铜管结垢或堵塞,降低冷却器换热能力,造成了同步电机定子绕组温度升高的情况,采取了对运行中的高炉电动鼓风机组同步电机水冷却器进行不停机反冲洗操作,实现清除冷却器... 详细信息

针对高炉电动鼓风机运行期间同步电机水冷却器内部由于泥沙污垢等使铜管结垢或堵塞,降低冷却器换热能力,造成了同步电机定子绕组温度升高的情况,采取了对运行中的高炉电动鼓风机组同步电机水冷却器进行不停机反冲洗操作,实现清除冷却器内部泥沙污垢,提升冷却器换热能力而保证电动鼓风机组安全稳定长周期经济运行,为高炉稳定生产提供了有力的保障。

关键词：高炉电动鼓风机同步电机水冷却器不停机反冲洗

Steady-migration retention characteristics of peptides under gradient elution： application towards a dynamic separation method for minor-adjustments of the retention of peptides in RPLC

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Science China Chemistry 2017年第6期60卷 829-836页

作者： Min Li Yongjun Lu yicong Yang Jianjun Li Lili wang Wei Tuo Xiaohui Ning Xin-Du Geng Institute of Modern Separation Science Shaanxi Provincial Key Laboratory Northwest University Xi'an 710069 China Xi'an Aolan Science and Technology Development Company Xi 'an 710075 China Xi'an Shiyou University Xi'an 710065 China

Minor-adjustment of the retention of peptides, induced by varying the mobile phase flow-rate(MPF-R), is a new dynamic separation method for simultaneously and rapidly identifying and improving the selectivity of hidden and overlapping peptide peaks. It can also-stabilize the reverse elution order of some pair-peaks under gradient elution in reverse phase liquid chromatography. The retention characteristics of peptides under gradient elution in RPLC was firstly found to be dominated by two variables of the steady region(SR) and migration region(MR). The changes in peptide retention induced by varying the MPF-R can be attributed to changes in the rate of bond breaking of multiple molecular interactions of peptides from the SR and of the mass transfer of peptides from the stationary phase to the mobile phase in the MR. The two dynamic variables were also found to independently depend on the type of peptide. Desirable results were obtained using six standard oligopeptides and a real sample of trypsin-digested *** is expected that the quality control of peptide drugs, high dispersion of peptide peaks in peptide mapping and "bottom-up MS"in proteomics will be improved by this method, even enabling peptide purification on a preparative scale in industry.

关键词： steady-migration retention liquid chromatography peptides dynamic separation minor-adjustment of retention elution orders

Co-delivery of TRAIL and paclitaxel by fibronectin-targeting liposomal nanodisk for effective lung melanoma metastasis treatment

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Nano Research 2022年第1期15卷 728-737页

作者： Shiqi Huang Lang Deng Hanming Zhang Luyao wang yicong Zhang Qing Lin Tao Gong Xun Sun Zhirong Zhang Ling Zhang Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of EducationWest China School of PharmacyCollege of Polymer Science and EngineeringSichuan UniversityChengdu 610041China

Melanoma is a highly aggressive cancer which often forms metastatic tumors in the lung,leading to sharply reduced patients'survival *** treating these tumors thus could improve late stage melanoma with lung *** this study,we fabricated a Cys-Arg-Glu-Lys-Ala with N-methylated Glu(CR(NMe)EKA)decorated disk shaped nano vehicle to co-deliver tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)and paclitaxel(PTX)to lung melanoma tumor sites(TRAIL-[ND-PTX]^(CR(NMe)EKA)).These nanodisks displayed better tumor-targeting and penetration capability than spherical nanoparticles,while the fibronectin-targeting CR(NMe)EKA motif also increased the tumor accumulation of loaded *** combined usage of TRAIL and PTX both killed tumor cells and reduced local nutrition supply,leading to stronger overall anti-tumor *** TRAIL-[ND-PTX]^(CR(NMe)EKA)system performed remarkably better than free paclitaxel and also significantly elongated survival rate of melanoma lung metastasis bearing mice,without displaying significant ***,this designing strategy and the fabricated nanoplatform possess potential for further development.

关键词： nanodisks Cys-Arg-Glu-Lys-Ala with N-methylated Glu(CR(NMe)EKA) tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) lung melanoma metastasis tumor-targeting

石家庄新型冠状病毒肺炎疫情新型冠状病毒分子流行病学特征

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中华检验医学杂志 2022年第6期45卷 637-641页

作者：高会霞杨林郭云王逸丛刘玉珍唐岳赫章梁鑫明黄顺楷高鹏黄颖戴慕巍张志胡倩王瑜玲陈芳戴二黑江平杜玉涛石家庄市第五医院检验科石家庄050021 石家庄医学检验实验室有限公司石家庄050000 中国科学院大学生命科学学院北京100049 河北医科大学第四医院骨科石家庄050010 石家庄市第五医院结核科石家庄050021 石家庄市第五医院内科石家庄050021

目的从分子层面分析石家庄新型冠状病毒的流行病学特征,揭示此次传播链的源头,为新型冠状病毒肺炎疫情防控提供科学依据。方法收集2021年1月2—8日来自石家庄市第五医院170例新型冠状病毒感染者共计404份新型冠状病毒核酸阳性样本,采用... 详细信息

目的从分子层面分析石家庄新型冠状病毒的流行病学特征,揭示此次传播链的源头,为新型冠状病毒肺炎疫情防控提供科学依据。方法收集2021年1月2—8日来自石家庄市第五医院170例新型冠状病毒感染者共计404份新型冠状病毒核酸阳性样本,采用多重聚合酶链反应靶向测序技术对样本进行深度测序获得病毒一致性序列。采用PANGOLIN分类系统对170个毒株序列进行分析,采用T-test对数据进行统计学分析。结果在404份新型冠状病毒核酸阳性样本中,356份获得病毒的全长基因组序列(>95%,100×测序深度),剔除重复取样,得到170株全基因组序列,所有样本均属于B.1.1分支。病毒突变数18~22个,大部分突变不改变氨基酸信息。根据基因组突变个数和种类,所有毒株分为48个亚型,其中2个毒株占主导(分别为66株和31株)。结论本研究中所有新型冠状病毒肺炎患者来自单一源头的境外输入性病例,病毒在社区隐匿传播了一段时间,并且在传播的过程中发生了变异。

关键词：新型冠状病毒新型冠状病毒肺炎多重PCR测序

Evaluation of Chito-Oligosaccharide(COS)in Vitro and in Vivo:Permeability Characterization in Caco-2 Cells Monolayer and Pharmacokinetics Properties in Rats

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Journal of Ocean University of China 2022年第3期21卷 782-788页

作者： ZHANG Pengpeng ZHANG Miaomiao DONG Kaiyu ZHANG yicong YANG Shuang wang Yuanhong JIANG Tingfu YU Mingming LV Zhihua School of Medicine and Pharmacy Ocean University of ChinaQingdao266003China Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao266003China Key Laboratory of Glycoscience&Glycotechnology of Shandong Province Qingdao266003China Key Laboratory of Marine Drugs Ministry of Education of ChinaQingdao266003China

Chito-oligosaccharide(COS)had shown a variety of biological activities and potential biomedical *** present study investigated the pharmacokinetics,bioavailability,and in vitro absorption of COS with degrees of polymerization(DPs)2-7 and explored the influence of DPs on *** Caco-2 cell permeation studies,COS were low permeability compounds with no directional effects,suggesting a low in vivo absorption mediated by facilitation diffusion and paracellular *** an intragastrical administration to rats,COS2 showed the highest systemic exposure in six *** bioavailability of COS2-7 was 7.33%,6.11%,4.67%,4.13%,4.02%,0.99%,*** in bioavailability for each COS correlated to structural variations,with high DPs contributing to a decrease in *** conclusion,COS could be absorbed by the intestinal tract both in vitro and in *** very low oral bioavailability of COS could be due to low *** can affect absorption and bioavailability of *** study provided evidence for the absorption characteristics of COS2-7 to help us better understanding the pharmacological actions.

关键词： chito-oligosaccharide(COS) Caco-2 transport pharmacokinetics bioavailability

Camptothecin multifunctional nanoparticles effectively achieve a balance between the efficacy of breast cancer treatment and the preservation of intestinal homeostasis

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Bioactive Materials 2024年第11期41卷 413-426页

作者： Qingya Liu Yun Yang Meng Pan Kun Shi Dong Mo yicong Li Meng wang Linfeng Guo Zhiyong Qian Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu610041China Department of Plastic and Burn Surgery West China HospitalSichuan UniversityChengdu610041China

Camptothecin(CPT)exhibits potent antitumor activity;however,its clinical application is limited by significant gastrointestinal adverse effects(GAEs).Although the severity of GAEs associated with CPT derivatives has decreased,the incidence rate of these adverse effects has remained *** multifunctional nanoparticles(PCRHNs)have the potential to increase the efficacy of CPT while reducing side effects in major target organs;however,the impact of PCRHNs on the GAEs from CPT remains ***,we investigated the therapeutic effects of PCRHNs and different doses of CPT and examined their impacts on the intestinal barrier and the intestinal *** found that the therapeutic efficacy of PCRHNs+Laser treatment was superior to that of high-dose CPT,and PCRHNs+Laser treatment also provided greater benefits by helping maintain intestinal barrier integrity,intestinal microbiota diversity,and intestinal microbiota *** summary,compared to high-dose CPT treatment,PCRHNs+Laser treatment can effectively balance therapeutic effects and GAEs.A high dose of CPT promotes the enrichment of the pathogenic bacteria Escherichia-Shigella,which may be attributed to diarrhea caused by CPT,thus leading to a reduction in microbial burden;additionally,Escherichia-Shigella rapidly grows and occupies niches previously occupied by other bacteria that are lost due to ***+Laser treatment increased the abundance of Lactobacillus(probiotics),possibly due to the photothermal effect of the *** effect increased catalase activity,thus facilitating the conversion of hydrogen peroxide into oxygen within tumors and increasing oxygen levels in the body,which is conducive to the growth of facultative anaerobic bacteria.

关键词： Camptothecin PCRHNs Intestinal barrier Intestinal microbiota Probiotics

Expansion and improvement of ChinaMu by MuT-seq and chromosome-level assembly of the Mu-starter genome

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Journal of Integrative Plant Biology 2024年第4期66卷 645-659页

作者： Lei Liang Yuancong wang Yanbin Han yicong Chen Mengfei Li Yibo Wu Zeyang Ma Han Zhao Rentao Song State Key Laboratory of Maize Bio-breeding Frontiers Science Center for Molecular Design BreedingJoint International Research Laboratory of Crop Molecular BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100091China Institute of Crop Germplasm and Biotechnology Jiangsu Provincial Key Laboratory of AgrobiologyJiangsu Academy of Agricultural SciencesNanjing 210014China Sanya Institute of China Agricultural University Sanya 572025China Hainan Yazhou Bay Seed Laboratory Sanya 572025China

ChinaMu is the largest sequence-indexed Mutator(Mu)transposon insertional library in maize(Zea mays).In this study,we made significant improvements to the size and quality of the ChinaMu *** developed a new Mu-tag isolation method Mu-Tn5-seq(MuT-seq).Compared to the previous method used by ChinaMu,MuT-seq recovered 1/3 more germinal insertions,while requiring only about 1/14 of the sequencing volume and 1/5 of the experimental *** MuT-seq,we identified 113,879 germinal insertions from 3,168 Mu-active F1 *** also assembled a high-quality genome for the Mu-active line Mu-starter,which harbors the initial active MuDR element and was used as the pollen donor for the mutation *** the Mu-starter genome,we recovered 33,662(15.6%)additional germinal insertions in 3,244(7.4%)genes in the Mu-starter *** Mu-starter genome also improved the assignment of 117,689(54.5%)germinal *** newly upgraded ChinaMu dataset currently contains 215,889 high-quality germinal *** insertions cover 32,224 pan-genes in the Mu-starter and B73Ref5 genomes,including 23,006(80.4%)core genes shared by the two *** a test model,we investigated Mu insertions in the pentatricopeptide repeat(PPR)superfamily,discovering insertions for 92%(449/487)of PPR genes in ChinaMu,demonstrating the usefulness of ChinaMu as a functional genomics resource for maize.

关键词： ChinaMu Mutator MuT-seq PPR gene Zea mays

Catalytic Asymmetric[4+1]Spiroannulation ofα-Bromo-βNaphthols with Azoalkenes by an Electrophilic Dearomatization/S_(RN)1-Debromination Approach

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CCS Chemistry 2022年第3期4卷 1054-1064页

作者： Lu Bai Xin Luo yicong Ge Hui wang Jingjing Liu Yaoyu wang Xinjun Luan Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education College of Chemistry&Materials ScienceNorthwest UniversityXi’an 710127 State Key Laboratory of Elemento-Organic Chemistry Nankai UniversityTianjin 300071

An enantioselective[4+1]spiroannulation ofαbromo-β-naphthols with azoalkenes has been developed for the one-step construction of a new class of pyrazoline-based spirocyclic *** chiral Cu(II)/Box catalysts,asymmetric induction was achieved with high levels of enantioselectivity[up to 99:1 enantiomeric ratio(er)].Notably,α-chloroandα-iodo-substitutedβ-naphthols were also tolerated by this *** studies disclosed that this process was triggered by electrophilefacilitated dearomatization ofα-bromo-β-naphthols and followed by the debromination via SRN 1-subsitution with in situ-formed *** chiral copper(II)-species,bound with azoalkene moiety,was assumed to control the enantiodiscrimination over the naphthoxy C-radical that was generated from the debromination ***,the potential utility of this protocol was greatly amplified by the derivatization of spirocyclic products through oxidative dearomatization of the other aromatic ring in the naphthyl fragment,providing a rather attractive route for the rapid generation of synthetically more valuable doubly dearomatized architectures.

关键词： dearomatization spiroannulation bromonaphthol azoalkene asymmetric catalysis