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Development of a recombinant pB602L-based indirect ELISA assay for detecting antibodies against African swine fever virus in pigs

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Journal of Integrative Agriculture 2022年第3期21卷 819-825页

作者： wang Peng-fei wang Ming SHI Zhi-bin SUN Zhen-zhao WEI Li-li LIU Zai-si wang Shi-da HE Xi-jun wang jing-fei State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

African swine fever(ASF),caused by the African swine fever virus(ASFV),is a devastating disease of domestic and wild *** is no effective vaccine,and the control of the disease relies mainly on surveillance and early detection of infected ***,serological assays,such as ELISA,have been developed mainly based on recombinant structural viral proteins of ASFV,including p72,p54,and ***,the antibodies against these proteins do not provide efficient protection against ASFV infection in ***,new serological assays that can be applied for clinical diagnosis and evaluating serological immune response in vaccinated pigs are still *** this study,we expressed and purified a recombinant p B602 L *** purified p B602 L protein was then used as an antigen to develop an indirect ELISA *** assay has no cross-reaction with the anti-sera against the 15 most common pig pathogens in China,such as classical swine fever virus,pseudorabies virus,and porcine *** assay and a commercial ELISA kit were then used to detect 60 field pig serum samples,including an unknown number of antiASFV *** coincidence of the two assays was 95%.Furthermore,the p B602 L-based ELISA was employed to test the antibody responses to the seven-gene-deleted ASFV strain HLJ/18-7 GD in *** results showed that the antibody levels in all vaccinated pigs,starting from the 10 th day post-inoculation,have increased continuously during the observation period of 45 *** results indicate that this p B602 L-based indirect ELISA assay can be employed potentially in the field of ASFV diagnosis.

关键词： African swine fever virus pB602L recombinant protein indirect ELISA assay pig

Development of the nano-composite cement:Application in regulating grouting in complex ground conditions

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Journal of Mountain Science 2018年第7期15卷 1572-1584页

作者： wang Sheng wang jing-fei YUAN Chao-peng CHEN Li-yi XU Shi-tong GUO Kai-bin State Key Laboratory of Geohazard Prevention & Geoenvironment Protection Chengdu University of Technology

Improvement of the fluidity and setting time of grouting materials has been recognized as an effective approach of seepage prevention in foundation works, and it is quite common to be used for handling severe leakages in complex ground conditions, such as loose, broken and fully fissured stratum. For the purposed of better meeting the engineering requirements, experimental studies were conducted in this study with focus on the nanocomposite grouting materials and the related controlled grouting technology. As compared with the commonly used silicate-sulpho-aluminate composite cement, which is characterized by relatively poor rheological property, quick setting time and low strength, the most suitable nano-material with proper reactants were selected intentionally to improve the mentioned attributes of composite cement. Due to the setting time and strength of the targeted cement slurry behaving with poor performance of harmonization to engineering construction problems, hydration synergistic effect of these composites were investigated in our experiments. Results showed that the properties of grouting materials, including initial fluidity, setting time, ideal right-angle thickening, and early strength and late strength were sufficient to produce an expected grouting application. It is therefore advocated that the refined grouting material could provide a better solution to fix grouting problems in complex ground cementing operations.

关键词： Nano-silica Silicate-sulpho-aluminate composite cement grout Controlled grouting Complex ground conditions

Phylogenetic and Structural Analysis of Major Surface Proteins Hemagglutinin and Neuraminidase of Novel Avian Influenza Virus A H7N9 from Chinese Patient

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Chemical Research in Chinese Universities 2013年第5期29卷 934-940页

作者： YUAN Xiao-hui wang Ying-chen QU Zhang-yi REN Jia-yi WU Xiao-min wang jing-fei Department of Microbiology Public Health College Harbin Medical University Harbin 150081 P. R. China Institute of Pathogen Biology Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100176 P R. China Key Laboratory of Resource and Plant Biology of Anhui Province College of Life Science Huaibei Normal University Huaibei 235000 P. R. China Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150081 P R. China

This research reveals the phylogenetic history and structural information of the hemagglutinin（HA） and neuraminidase（NA） from novel avian influenza virus A/Hangzhou/1/2013（HTN9_2013） strain from human infected in China. Strains closely related to the HTN9 2013 strain were obtained from Nation Center for Biotechnology Information（USA）-basic local alignment search tool（NCBI-BLAST） searching, and the phylogenetic trees were con- structed. The 3D structures of HA and NA from H7N9 2013 strain were built by homology modeling technology, and molecular dynamics（MD） simulations were performed on the high-performance computer cluster. Characteristic amino acid sites were then screened from multiple sequence alignment（MSA） via home-made Python script and mapped onto the 3D structures. The thermodynamic characteristic root-mean-square-fluctuation （RMSF） of these sites in the structure was also analyzed with MD trajectories. The HA of HTN9_2013 strain is closely related to the A/duck/Zhejiang/12/2011 strain isolated in China, while the NA of HTN9 2013 strain is mostly related to the A/mallard/Czech Republic/13438-29K/2010 strain isolated in Europe. The 3D structures of HA and NA from H7N9 2013 stain are mostly identical to the existing structure of H7 and N9. A total of 11 and 14 characteristic ami- no acid sites were identified in HA and NA, respectively, in HTN9_2013. Structural analysis indicates that certain sites in the top region of HA are important, at which the mutation of some amino acids can impact the receptor bin- ding that may be related to its infection of human beings.

关键词： Avian influenza virus Hemagglutinin Neuraminidase Homology modeling Phylogenetic

Establishment of a Risk Assessment Framework for Analysis of the Spread of Highly Pathogenic Avian Influenza

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Agricultural Sciences in China 2007年第7期6卷 877-881页

作者： LI jing wang jing-fei WU Chun-yan YANG Yan-tao JI Zeng-tao wang Hong-bin National Key Laboratory of Veterinary Biotechnology/Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150001 P.R.China College of Veterinary Medicine Northeast Agricultural University Harbin 150030 P.R.China

To evaluate the risk of highly pathogenic avian influenza （HPAI） in China's Mainland, a risk assessment framework was built. Risk factors were determined by analyzing the epidemic data using the brainstorming method; the analytic hierarchy process was designed to weigh risk factors, and the integrated multicriteria analysis was used to evaluate the final result. The completed framework included the risk factor system, data standards for risk factors, weights of risk factors, and integrated assessment methods. This risk assessment framework can be used to quantitatively analyze the outbreak and spread of HPAI in China's Mainland.

关键词： highly pathogenic avian influenza (HPAI) risk factor risk assessment framework analytical hierarchy process (AHP) weights integrated assessment

Co-production of hydrogen, oxygen, and electricity via an integrated solar-driven system with decoupled water electrolyzer and Na-Zn ion battery

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Journal of Energy Chemistry 2025年第1期100卷 621-627页

作者： fei Lv Longjie Liu Jiazhe Wu Pengfei wang Lixia Pan Dengwei jing Yubin Chen International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University

Combining water electrolysis and rechargeable battery technologies into a single system holds great promise for the co-production of hydrogen （H2） and ***,the design and development of such systems is still in its ***,an integrated hydrogen-oxygen （O2）-electricity co-production system featuring a bipolar membrane-assisted decoupled electrolyzer and a Na-Zn ion battery was established with sodium nickelhexacyanoferrate （NaNiHCF） and Zn2+/Zn as dual redox *** decoupled electrolyzer enables to produce H2and O2in different time and space with almost 100%Faradaic efficiency at 100 mA ***,the charged NaNiHCF and Zn electrodes after the electrolysis processes formed a Na-Zn ion battery,which can generate electricity with an average cell voltage of 1.75 V at 10 m A *** connecting Si photovoltaics with the modular electrochemical device,a well-matched solar driven system was built to convert the intermittent solar energy into hydrogen and electric energy with a solar to hydrogen-electricity efficiency of 16.7%,demonstrating the flexible storage and conversion of renewables.

关键词： Hydrogen Electricity Decoupled water electrolyzer Na-Zn ion battery Solar energy

Virucidal activity of MICRO-CHEM PLUS against African swine fever virus

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Journal of Integrative Agriculture 2023年第11期22卷 3560-3563页

作者： JIANG Cheng-gang SUN Ying ZHANG Fan AI Xin LU Ming QIN Jia-lin ZHANG Xian-feng wang jing-fei BU Zhi-gao ZHAO Dong-ming HE Xi-jun State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China National High Containment Laboratory for Animal Diseases Control and Prevention Harbin 150069P.R.China

African swine fever(ASF) was first discovered and reported in East Africa in 1921 and it has been listed as a notifiable animal disease by the World Organization for Animal Health(WOAH) due to the high morbidity and mortality(WOAH 2019). African swine fever virus(ASFV) can persist in pig farm environments, carcasses, and various pig products(Petrini et al. 2019). ASF has caused significant economic losses to the pig industry since its outbreak in China in 2018(Zhao et al. 2019).

关键词： swine fever listed

数字产业化、金融科技创新与实体经济“脱实向虚”

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商业研究 2023年第3期 30-38页

作者：王婧菲孙立东南大学马克思主义学院南京211189 东南大学能源与环境学院南京211189

发挥数字产业化的金融引导功能是抑制实体经济“脱实向虚”的重要选择。以2011-2020年沪深A股上市公司为样本,基于产权性质差异构建双重差分模型,检验数字产业化对金融科技创新与实体经济“脱实向虚”的影响及具体路径。研究表明:数字... 详细信息

发挥数字产业化的金融引导功能是抑制实体经济“脱实向虚”的重要选择。以2011-2020年沪深A股上市公司为样本,基于产权性质差异构建双重差分模型,检验数字产业化对金融科技创新与实体经济“脱实向虚”的影响及具体路径。研究表明:数字产业化可促进金融科技创新水平提升,并抑制实体经济“脱实向虚”。同时,金融科技创新是数字产业化抑制实体经济“脱实向虚”的关键渠道。进一步研究显示,在金融开放水平较低地区,数字产业化对金融科技创新效应驱动效果更强。应大力孵化金融科技数字新基建、适度调整数字经济发展杠杆、拓展数字普惠金融服务边界,助力实体经济高质量发展。

关键词：数字产业化金融科技创新实体经济脱实向虚双重差分

Optimizing the key parameter to accelerate the recovery of AMOC under a rapid increase of greenhouse gas forcing

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 39-45页

作者： Haolan Ren fei Zheng Tingwei Cao Qiang wang International Center for Climate and Environment Science(ICCES) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina College of Oceanography Hohai UniversityNanjingChina CAS Key Laboratory of Ocean Circulation and Waves Institute of OceanologyChinese Academy of Sciences and Pilot National Laboratory for Marine Science and Technology(Qingdao)QingdaoChina

Atlantic Meridional Overturning Circulation(AMOC)plays a central role in long-term climate variations through its heat and freshwater transports,which can collapse under a rapid increase of greenhouse gas forcing in climate *** studies have suggested that the deviation of model parameters is one of the major factors in inducing inaccurate AMOC *** this work,with a low-resolution earth system model,the authors try to explore whether a reasonable adjustment of the key model parameter can help to re-establish the AMOC after its *** a new optimization strategy,the extra freshwater flux(FWF)parameter is determined to be the dominant one affecting the AMOC’s *** traditional ensemble optimal interpolation(EnOI)data assimilation and new machine learning methods are adopted to optimize the FWF parameter in an abrupt 4×CO_(2) forcing experiment to improve the adaptability of model parameters and accelerate the recovery of *** results show that,under an abrupt 4×CO_(2) forcing in millennial simulations,the AMOC will first collapse and then re-establish by the default FWF parameter ***,during the parameter adjustment process,the saltier and colder sea water over the North Atlantic region are the dominant factors in usefully improving the adaptability of the FWF parameter and accelerating the recovery of AMOC,according to their physical relationship with FWF on the interdecadal timescale.

关键词： Recovery of AMOC 4×CO_(2)forcing Key parameter Parameter estimation Data assimilation Machine learning

重思青年马克思与黑格尔的哲学关系——历史与文本中的《1844年经济学哲学手稿》再考察

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江苏行政学院学报 2024年第2期 20-26页

作者：王婧菲凤启龙东南大学马克思主义学院南京210096

马克思在《1844年经济学哲学手稿》中对黑格尔辩证法的批判是判断两者理论关系的重要文本基础,也是国际学术界最富争议性的话题之一。从历史来看,《1844年手稿》中[对黑格尔的辩证法和整个哲学的批判]经历了《马克思恩格斯文库》的首次... 详细信息

马克思在《1844年经济学哲学手稿》中对黑格尔辩证法的批判是判断两者理论关系的重要文本基础,也是国际学术界最富争议性的话题之一。从历史来看,《1844年手稿》中[对黑格尔的辩证法和整个哲学的批判]经历了《马克思恩格斯文库》的首次收录、MEGA1的系统性编纂、《历史唯物主义早期文选》的非顺序性编辑、MEGA2的逻辑与写作双顺序编辑等历史时期,在不同版本中呈现出与其相应的意识形态话语相一致的编辑顺序,并引发了持续的争议。从思想史来看,青年马克思与黑格尔的哲学关系呈现出复杂且多变的状态,随着马克思研究的推进,他对黑格尔的态度也发生着一种辩证性的运动,而《1844年手稿》则是其思想转变过程中的关键节点之一。从文本来看,马克思在《1844年手稿》中对黑格尔的批判不仅仅局限于[对黑格尔的辩证法和整个哲学的批判],对两者哲学关系的再考察要放到更广阔的思想背景和文本内容中去重新思考。

关键词：青年马克思黑格尔《1844年经济学哲学手稿》思想史