Phylogenetic and Structural Analysis of Major Surface Proteins Hemagglutinin and Neuraminidase of Novel Avian Influenza Virus A H7N9 from Chinese Patient
Phylogenetic and Structural Analysis of Major Surface Proteins Hemagglutinin and Neuraminidase of Novel Avian Influenza Virus A H7N9 from Chinese Patient作者机构：Department of Microbiology Public Health College Harbin Medical University Harbin 150081 P. R. China Institute of Pathogen Biology Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100176 P R. China Key Laboratory of Resource and Plant Biology of Anhui Province College of Life Science Huaibei Normal University Huaibei 235000 P. R. China Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150081 P R. China
出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)
年 卷 期：2013年第29卷第5期
页 面：934-940页
核心收录：
学科分类：090601[农学-基础兽医学] 08[工学] 080401[工学-精密仪器及机械] 09[农学] 0906[农学-兽医学] 0804[工学-仪器科学与技术] 0803[工学-光学工程]
基 金：Supported by the National Natural Science Foundation of China(Nos.81172725 30271175)
主 题：Avian influenza virus Hemagglutinin Neuraminidase Homology modeling Phylogenetic
摘 要：This research reveals the phylogenetic history and structural information of the hemagglutinin（HA） and neuraminidase（NA） from novel avian influenza virus A/Hangzhou/1/2013（HTN9_2013） strain from human infected in China. Strains closely related to the HTN9 2013 strain were obtained from Nation Center for Biotechnology Information（USA）-basic local alignment search tool（NCBI-BLAST） searching, and the phylogenetic trees were con- structed. The 3D structures of HA and NA from H7N9 2013 strain were built by homology modeling technology, and molecular dynamics（MD） simulations were performed on the high-performance computer cluster. Characteristic amino acid sites were then screened from multiple sequence alignment（MSA） via home-made Python script and mapped onto the 3D structures. The thermodynamic characteristic root-mean-square-fluctuation （RMSF） of these sites in the structure was also analyzed with MD trajectories. The HA of HTN9_2013 strain is closely related to the A/duck/Zhejiang/12/2011 strain isolated in China, while the NA of HTN9 2013 strain is mostly related to the A/mallard/Czech Republic/13438-29K/2010 strain isolated in Europe. The 3D structures of HA and NA from H7N9 2013 stain are mostly identical to the existing structure of H7 and N9. A total of 11 and 14 characteristic ami- no acid sites were identified in HA and NA, respectively, in HTN9_2013. Structural analysis indicates that certain sites in the top region of HA are important, at which the mutation of some amino acids can impact the receptor bin- ding that may be related to its infection of human beings.
维普期刊数据库