Development of a recombinant pB602L-based indirect ELISA assay for detecting antibodies against African swine fever virus in pigs

作     者：WANG Peng-fei WANG Ming SHI Zhi-bin SUN Zhen-zhao WEI Li-li LIU Zai-si WANG Shi-da HE Xi-jun WANG Jing-fei WANG Peng-fei;WANG Ming;SHI Zhi-bin;SUN Zhen-zhao;WEI Li-li;LIU Zai-si;WANG Shi-da;HE Xi-jun;WANG Jing-fei

作者机构：State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第3期

页      面：819-825页

核心收录：

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by grants from the National Key R&D Program of China (2016YFD0500705, 2017YFD0500105, and 2017YFC1200502) the Fundamental Research Funds for the Central Publicinterest Research Institutes (Y2017LM08) 

主　　题：African swine fever virus pB602L recombinant protein indirect ELISA assay pig 

摘      要：African swine fever(ASF),caused by the African swine fever virus(ASFV),is a devastating disease of domestic and wild *** is no effective vaccine,and the control of the disease relies mainly on surveillance and early detection of infected ***,serological assays,such as ELISA,have been developed mainly based on recombinant structural viral proteins of ASFV,including p72,p54,and ***,the antibodies against these proteins do not provide efficient protection against ASFV infection in ***,new serological assays that can be applied for clinical diagnosis and evaluating serological immune response in vaccinated pigs are still *** this study,we expressed and purified a recombinant p B602 L *** purified p B602 L protein was then used as an antigen to develop an indirect ELISA *** assay has no cross-reaction with the anti-sera against the 15 most common pig pathogens in China,such as classical swine fever virus,pseudorabies virus,and porcine *** assay and a commercial ELISA kit were then used to detect 60 field pig serum samples,including an unknown number of antiASFV *** coincidence of the two assays was 95%.Furthermore,the p B602 L-based ELISA was employed to test the antibody responses to the seven-gene-deleted ASFV strain HLJ/18-7 GD in *** results showed that the antibody levels in all vaccinated pigs,starting from the 10 th day post-inoculation,have increased continuously during the observation period of 45 *** results indicate that this p B602 L-based indirect ELISA assay can be employed potentially in the field of ASFV diagnosis.