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第十一届全国免疫学学术大会

作者： Cai Mengying wang Cheng Gu Hongjing Duan Yueqiang Lai Chengcai wang Keyu Yang Penghui wang xiliang Beijing Institute of Microbiology and epidemiology Beijing 307 Hospital Affiliated to Academy of Medical Sciences Beijing 302 Hospital

Objective: Respiratory syncytial virus(RSV) is leading cause of lower respiratory infection in infants and children, but there is still no licensed vaccine availble. Virus-like partice(VLP)is a novel approach for RSV vaccine which form a virus-like structure but lack the viral ***: We designed VLP vaccines consist of influenza virus matrix(M1)protein and RSV fusion protein(F) or glycoprotein(G).These VLPs were develped based on the Bac-to-Bac baculovirus expression systerm and identified by western blot and electron microscoy. Finally,protective efficacy of the VLPs were evaluated using BALB/c ***:We successfully developed RSV-F and RSV-G VLPs. Morphology and sizes of VLPs were consistent with that of the wild-type *** BALB/c mice immunized intranaslly(i.n.) with RSV-F VLPs,RSV-G VLPs, or both showed viral-specific antibody responses against RSV. Total IgG, IgG1,IgG2 a,and mucosal IgA were detected in mice with RSV-F plus RSV-G VLPs, revealing potent cellular and mucosal immune responses. Moreover, we found that those mixed RSV VLPs conferred enhanced protection against live RSV challenges,showing significant decreases in lung viral replication and obvirous attenuation of histopathological changes associated with viral ***: RSV-F plus RSV-G VLPs by intransal vaccination as a promisingvaccine candidate, which warrants furhter evaluation using cotton rat and primate models.

关键词： Respiratory syncytial virus VLP Intranasal vaccination Fusion protein Glycoprotein

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Influenza virus vaccine expressing fusion and attachment protein epitopes of respiratory syncytial virus induces protective antibodies in vivo

Influenza virus vaccine expressing fusion and attachment pro...

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第九届全国免疫学学术大会

作者： Penghui Yang Chengrong Bian Yueqiang Duan Hongjing Gu Shaogeng Zhang xiliang wang Beijing Institute of Microbiology and Epidemiology State Key Laboratory of Pathogen and Biosecurity Beijing302 Hospital

Respiratory syncytial virus(RSV)is an important viral pathogen that causes life-threatening respiratory infections in both infants and the elderly;no vaccines are at present *** this report,we examined the use of infl...

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超高压电网无功平衡实用计算

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电力自动化设备 2008年第7期28卷 62-65页

作者：周会平王熙亮温渤婴中国农业大学信息与电气工程学院北京100083 国网信息通信有限公司北京100761

介绍了以直流潮流为基础,结合最优无功补偿理论的超高压电网无功平衡计划实用计算方法。该方法根据电网有功日负荷预测值,按照实际的电网拓扑结构和运行方式,首先计算线路和变压器等电网元件的等值电路参数,生成导纳矩阵,通过直流潮流... 详细信息

介绍了以直流潮流为基础,结合最优无功补偿理论的超高压电网无功平衡计划实用计算方法。该方法根据电网有功日负荷预测值,按照实际的电网拓扑结构和运行方式,首先计算线路和变压器等电网元件的等值电路参数,生成导纳矩阵,通过直流潮流计算支路有功潮流和无功损耗,然后按照无功分点在线路中点的优化理论,快速优化各节点无功补偿量。算例表明,该实用算法可避免重复迭代和潮流不收敛问题。

关键词：直流潮流最优无功补偿超高压电网:无功平衡计划

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维普期刊数据库

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Immunization with a live attenuated H7N9 influenza vaccine protects mice against lethal challenge

Immunization with a live attenuated H7N9 influenza vaccine p...

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第十届全国免疫学学术大会

作者： Yueqiang Duan Penghui Yang Xiaolan Yang Zhongpeng Zhao Hongjing Gu xiliang wang Beijing Institute of Microbiology and Epidemiology 302 hospitial

The emergence of severe cases of human influenza A(H7N9) viral infection in China in the spring of 2003 resulted in a global effort to rapidly develop an effective candidate vaccine. In this report, using reverse genetic technology, we generated a cold-adapted(ca), live attenuated monovalent reassortant influenza H7N9 virus(Ah01/AA ca) that contained hemagglutinin(HA) and neuraminidase(NA) genes from a 2013 pandemic A H7N9 isolate, A/Anhui/01/2013 virus(Ah01/H7N9);the remaining six internal gene segments came from the cold-adapted influenza H2N2 A/Ann Arbor/6/60 virus(AA virus). Ah01/AA ca exhibited phenotypes of temperature sensitivity(ts), ca, and attenuation(att). Intranasal immunization of female BALB/c mice with Ah01/AA ca twice at a 2-week interval induced robust humoral, mucosal, and cellmediated immune responses in a dose-dependent manner. Furthermore, the candidate Ah01/AA ca virus was immunogenic and offered partial or complete protection of mice against a lethal challenge by the live 2013 influenza A H7N9(A/Anhui/01/2013). Protection was demonstrated by the inhibition of viral replication and the attenuation of histopathological changes in the challenged mouse lung. Taken together, these data support the evaluation of this Ah01/AA ca candidate vaccine for human use in clinical trials.

关键词： H7N9 live attenuated vaccine cold-adapted

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文学他国化与“变文格义”:隋唐佛学中的变异思想

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暨南学报（哲学社会科学版） 2020年第5期42卷 1-11页

作者：曹顺庆王熙靓四川大学文学与新闻学院

变异学的重要创新理论之一,是文学他国化研究。"变文"是伴随着佛教中国化、世俗化的进程出现的一种说唱题材的通俗文学作品。它作为中国古代文明与印度文明互鉴与交流的成功实践,是异质文明之间相互对话碰撞,并最终在文学观念上融合创... 详细信息

变异学的重要创新理论之一,是文学他国化研究。"变文"是伴随着佛教中国化、世俗化的进程出现的一种说唱题材的通俗文学作品。它作为中国古代文明与印度文明互鉴与交流的成功实践,是异质文明之间相互对话碰撞,并最终在文学观念上融合创新的产物,是变异学的重要案例。"格义"是产生于中国佛教史上的概念,其基本含义是指汉魏两晋时期一些佛教学者用中国本土固有的思想去比附印度佛教概念的方法。本文拟通过分析"变文"与"格义"中蕴藏的变异思想,以论证隋唐佛学中的"他国化"变异在碰撞交流的基础之上催发新的文化现象,从而论证"他国化"不仅能解决历史上的中国文学如何实现"中国化"的重大现实问题,而且能促进文明互鉴、文化的碰撞交流与文化创新。

关键词：变文格义变异学文明互鉴

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维普期刊数据库

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C-C motif chemokine ligand 2(CCL2) mediates acute lung injury induced by lethal influenza H7N9 virus

C-C motif chemokine ligand 2(CCL2) mediates acute lung injur...

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第十一届全国免疫学学术大会

作者： Lai Chengcai wang Keyu Gu Hongjing Zhao Zhongpeng Zhang Liangyan Xing Li Yang Penghui wang xiliang State Key Laboratory of Pathogens and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 302 Hospital

An avian-origin influenza A(H7N9) virus has raised concern in China in the spring of 2013. Most H7N9 infections cases resulted in acute respiratory distress syndrome(ARDS), which is a severe form of acute lung injury(ALI) that further contributes to morbidity and mortality. In this study,we report a close association of CCL2 expression and acute lung injury induced by lethal H7N9 virus strain(A/Hebei/01/2013) in a mouse model. Elevated CCL2 levels were detected inconfirmed H7N9 patients and the bronchoalveolarlavage fluid(BALF) of H7N9 infected mice. Moreover, the overexpressions of CCL2 were observed in lung tissue of infected mice post infection. More importantly, CCL2 deficiency ameliorated acute lung injury induced by H7N9 virus in mice as determined by weight loss, survival rates, lung wet dry ratio and *** together, our findings demonstrated that CCL2 were essential for H7N9 virus infection andinterfering CCL2 might be promising as a potential therapeutic target for future influenza pandemic.

关键词： H7N9 CCL2 ALI

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Basic fibroblast growth factor(FGF2) plays a crucial role in influenza A virus-induced acute lung injury

Basic fibroblast growth factor(FGF2) plays a crucial role in...

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第十一届全国免疫学学术大会

作者： wang Keyu Lai Chengcai Gu Hongjing Zhao Zhongpeng Duan Yueqiang Yang Xiaolan Yang Penghui wang xiliang State Key Laboratory of Pathogens and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 302 Hospital of PLA

Influenza virus(IFV) infection is a major cause of severe respiratory illness that affects almost every country in the world. IFV infections result in respiratory illness and even acute lung injury and death, but the underlying mechanisms responsible for IFV pathogenesis remain to be fully elucidated. In this study, we found that the basic fibroblast growth factor 2(FGF2) level was markedly increased in H1N1 virus-infected humans and mice. FGF2, which is predominately derived from epithelial cells, recruits and activates neutrophils via the FGFR2-PI3K-AKT-NFκB signaling pathway. FGF2 depletion or knockoutexacerbated influenza-associated disease by impairing neutrophil recruitment and activation. More importantly, administration of recombinant FGF2 protein significantly alleviated the severity of IFV-induced lunginjury and promotedthe survival of IFV-infected animals. These findings demonstrate that FGF2 plays a critical role in preventing IFV-induced lung injury and suggest FGF2 as a promising potential therapeutic target during IFV infection.

关键词： H1N1 FGF2 neutrophils NFκB signaling therapy

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Long noncoding RNA AVAN mediates antiviral innate immune response via enhance the deubiquitination of TRIM25 and expression of FOXO3a

Long noncoding RNA AVAN mediates antiviral innate immune res...

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第十二届全国免疫学学术大会

作者： Lai Chengcai wang Keyu Gu Hongjing Zhao Lingna Zhou Shanshan Xia Min Yang Penghui xiliang wang Beijing Institute of Microbiology and Epidemiology State Key Laboratory of Pathogen and Biosecurity Beijing 302 Hospital of PLA

Long noncoding RNAs(IncRNAs)regulate a number of biological process and immune ***,the role of IncRNAs in innate immune responses remained largely ***,we indentified a uncharacterized

关键词： long noncoding RNA AVAN virus TRIM25 FOX03a

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Bivalent vaccine platform based on influenza virus elicits protective immune response against FLU and hepatitis C virus

Bivalent vaccine platform based on influenza virus elicits p...

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第十一届全国免疫学学术大会

作者： Penghui Yang Tianyu Ren Fang Sun Hongjing Gu Chengcai Lai Shaogeng Zhang xiliang wang Beijing 302 Hospital Beijing Institute of Microbiology and Epidemiology State Key Laboratory of Pathogen and Biosecurity

Hepatitis C virus(HCV)has the devastating impact of endanger human health,which can evolve into liver fibrosis,cirrhosis as well as hepatocellular *** now,an effective HCV vaccine is not *** this study,we generated a recombinant PR8 influenza virus vector carrying the core and envelope glycoproteins(C/E1/E2)epitopes of HCV inserted into the influenza nonstructural(NS1)protein gene,termed as ***

关键词： Influenza virus HCV Hexon protein epitopes viral vector

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Immunogenicity of an influenza virus-vectored vaccine carrying the hepatitis C virus protein epitopes in mice

Immunogenicity of an influenza virus-vectored vaccine carryi...

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第十二届全国免疫学学术大会

作者： Yang penghui Sun Fang Duan Yueqiang Gu Hongjing Zhang Peirui zhang Shaogeng wang xiliang Beijing 302 Hospital State Key Laboratory of Pathogens and Biosecurity Beijing Institute of Microbiology and Epidemiology

Hepatitis C virus(HCV) has a devastating impact on human health,and infections can progress into liver fibrosis,cirrhosis,and hepatocellular *** is no effective HCV *** this study,we rescued a recombinant PR8 influenza viral vector,called rgFLU-HCV,carrying the core and envelope glycoprotein(C/E1/E2) epitopes of HCV inserted into the influenza nonstructural protein 1 *** morphological characteristics of rgFLU-HCV and the expression of the C/E1/E2 epitopes of HCV were ***-HCV replicated in various cell lines,including MDCK,A549,and Huh7.5 *** importantly,in BALB/c mice immunized intranasally twice at a 21-day interval with 10,10,or 10 TCID rgFLU-HCV,the viral vector induced a robust antibody response to influenza and HCV and potent IFN-γ and IL-4 secretion in response to HCV antigens in a dose-dependent *** rgFLU-HCV virus also stimulated IFN-γ production by virus-specific peripheral blood mononuclear cells in patients with chronic HCV *** study demonstrated that rgFLU-HCV carrying HCV antigens is immunogenic in vivo and has potential for the development of a HCV vaccine.

关键词： Influenza virus vector HCV epitopes

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