Virus-like particle vaccine by intranasal vaccination elicits protecitive immunity against respiratory syncytial virus infection in mice
作者单位：Beijing Institute of Microbiology and epidemiology Beijing 307 HospitalAffiliated to Academy of Medical Sciences Beijing 302 Hospital
会议名称：《第十一届全国免疫学学术大会》
会议日期：2016年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
关 键 词：Respiratory syncytial virus VLP Intranasal vaccination Fusion protein Glycoprotein
摘 要：Objective: Respiratory syncytial virus(RSV) is leading cause of lower respiratory infection in infants and children, but there is still no licensed vaccine availble. Virus-like partice(VLP)is a novel approach for RSV vaccine which form a virus-like structure but lack the viral ***: We designed VLP vaccines consist of influenza virus matrix(M1)protein and RSV fusion protein(F) or glycoprotein(G).These VLPs were develped based on the Bac-to-Bac baculovirus expression systerm and identified by western blot and electron microscoy. Finally,protective efficacy of the VLPs were evaluated using BALB/c ***:We successfully developed RSV-F and RSV-G VLPs. Morphology and sizes of VLPs were consistent with that of the wild-type *** BALB/c mice immunized intranaslly(i.n.) with RSV-F VLPs,RSV-G VLPs, or both showed viral-specific antibody responses against RSV. Total IgG, IgG1,IgG2 a,and mucosal IgA were detected in mice with RSV-F plus RSV-G VLPs, revealing potent cellular and mucosal immune responses. Moreover, we found that those mixed RSV VLPs conferred enhanced protection against live RSV challenges,showing significant decreases in lung viral replication and obvirous attenuation of histopathological changes associated with viral ***: RSV-F plus RSV-G VLPs by intransal vaccination as a promisingvaccine candidate, which warrants furhter evaluation using cotton rat and primate models.