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Temporal Transcriptional Regulation of IL-10-Induced Anti-Inflammatory Genes in LPS-Triggered Macrophages

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Open j.urnal of Immunology 2014年第3期4卷 96-116页

作者： Amanda F. Dillow Leah N. Cardwell tyler j. Smith Brad D. Groppe Brian A. Peterson Maxwell A. Sickman Brian K. Weaver 不详

Interleukin-10 (IL-10) mediates an anti-inflammatory response that is executed through the expression of IL-10-induced genes. Certain IL-10-induced genes, exemplified by TNIP3, are induced by IL-10 only in conj.nction with a pro-inflammatory signal. We sought to characterize the mechanism whereby IL-10 and Toll-like receptor signals synergized to induce expression of genes like TNIP3 inmacrophages. Stimulation with IL-10 and lipopolysaccharide (LPS) synergistically induced an increase in the transcription rate of TNIP3, while having no effect on its mRNA stability. This transcriptional mechanism proved to be generalizable to 14 other genes that also were synergistically induced by IL-10 and LPS in monocytes/macrophages. Although all of the genes had this synergistic transcriptional regulation in common, they could be divided into three subsets based on their differential requirements for de novo protein synthesis and kinetics of expression: namely, primary response genes, early secondary response genes, and late secondary response genes. This coordinated and temporal pattern of transcriptional regulation in response to IL-10 and LPS was conserved in both human and mouse monocytes/macrophages, and it was associated with differential dependencies on PI3K and j.K signaling pathways. These results underscore the complex nature of the IL-10-induced transcriptional response that occurs specifically in LPS-triggered macrophages.

关键词： IL-10 TLR Macrophage Inflammation Transcription

Maize Carbohydrate Partitioning Defective33 Encodes an MCTP Protein and Functions in Sucrose Export from Leaves

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Molecular Plant 2019年第9期12卷 1278-1293页

作者： Thu M.Tran tyler j.McCubbin Saadia Bihmidine Benj.min T.j.lius R.Frank Baker Martin Schauflinger Clifford Weil Nathan Springer Paul Chomet Ruth Wagner j.ff Woessner Karen Grote j.anette Peevers Thomas L.Slewinski David M.Braun Division of Biological Sciences’Interdisciplinary Plant Group Missouri Maize CenterUniversity of MissouriColumbiaMO 65211USA Division of Plant Sciences University of MissouriColumbiaMO 65211USA Electron Microscopy Core Facility University of MissouriColumbiaMO 65211USA Department of Agronomy Purdue UniversityWest LafayetteIN 47907USA Department of Plant and Microbial Biology University of MinnesotaSaint PaulMN 55108USA NRGene Inc. 8910 University Center Lane\r\n S u ite 400San DiegoCA 92122USA Bayer Crop Science ChesterfieldMO 63017USA National Key Laboratory for Plant Cell Technology Agricultural Genetics InstituteHanoiVietnam

To sustain plant growth,development,and crop yield,sucrose must be transported from leaves to distant parts of the plant,such as seeds and *** identify genes that regulate sucrose accumulation and transport in maize(Zea mays),we isolated carbo/iydrafe part/f/ofi/ngf defecf/Ve33(cpd33),a recessive mutant that accumulated excess starch and soluble sugars in mature *** cpd33 mutants also exhibited chlorosis in the leaf blades,greatly diminished plant growth,and reduced ***33 encodes a protein containing multiple C2 domains and transmembrane *** localization experiments showed the CPD33 protein localized to plasmodesmata(PD),the plasma membrane,and the endoplasmic *** also found that a loss-of-function mutant of the CPD33 homolog in Arabidopsis,QUIRKY,had a similar carbohydrate hyperaccumulation *** labeled sucrose transport assays showed that sucrose export was significantly lower in cpd33 mutant leaves relative to wild-type ***,PD transport in the adaxial-abaxial direction was unaffected in cpd33 mutant ***,transmission electron microscopy revealed fewer PD at the companion cell-sieve element interface in mutant phloem tissue,providing a possible explanation for the reduced sucrose export in mutant ***,our results suggest that CPD33 functions to promote symplastic transport into sieve elements.

关键词： carbohydrate accumulation companion cells phloem, plasmodesmata sieve elements symplastictransport

Evolving technologies for growing,imaging and analyzing 3D root system architecture of crop plants

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j.urnal of Integrative Plant Biology 2016年第3期58卷 230-241页

作者： Miguel A.Pieros Brandon G.Larson j.n E.Shaff David j.Schneider Alexandre Xavier Falcao Lixing Yuan Randy T.Clark Eric j.Craft tyler W.Davis Pierre-Luc Pradier Nathanael M.Shaw Ithipong Assaranurak Susan R.Mc Couch Craig Sturrock Malcolm Bennett Leon V.Kochian USDA-ARS Robert Holley Center for Agriculture and Health538 Tower RoadIthacaNY 14580USA Department of Information Systems Institute of ComputingUniversity of CampinasAv.Albert Einstein1251CEP 13083-852CampinasSPBrazil Department of Plant Nutrition China Agricultural UniversityBeijing 100193China Department of Plant Breeding and Genetics Cornell UniversityIthacaNY 14853USA Centre for Plant Integrative Biology School of BiosciencesUniversity of NottinghamSutton BoningtonLE12 5RDUK College of Science King Saud UniversityRiyadh 11451Kingdom of Saudi Arabia

A plant＇s ability to maintain or improve its yield under limiting conditions,such as nutrient de ficiency or drought,can be strongly in fluenced by root system architecture（RSA）,the three-dimensional distribution of the different root types in the soil. The ability to image,track and quantify these root system attributes in a dynamic fashion is a useful tool in assessing desirable genetic and physiological root traits. Recent advances in imaging technology and phenotyping software have resulted in substantive progress in describing and quantifying RSA. We have designed a hydroponic growth system which retains the three-dimensional RSA of the plant root system,while allowing for aeration,solution replenishment and the imposition of nutrient treatments,as well as high-quality imaging of the root system. The simplicity and flexibility of the system allows for modi fications tailored to the RSA of different crop species and improved throughput. This paper details the recent improvements and innovations in our root growth and imaging system which allows for greater image sensitivity（detection of fine roots and other root details）,higher ef ficiency,and a broad array of growing conditions for plants that more closely mimic those found under field conditions.

关键词： Abiotic stress digital root phenotyping mineral nutrition root system architecture

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250例经血清学证实为西尼罗河病毒性脑膜炎和脑炎患者的CSF检查结果

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世界核心医学期刊文摘(神经病学分册) 2006年第10期 32-33页

作者： tyler K.L. Pape j. GoodyR.j. 赵天智 Neurology B- 182 University of Colorado Health Sciences Center 4200 East 9th Avenue Denver CO 80262 United States Dr.

Obj.ctive: To provide a large, comprehensive evaluation of the CSF findings in patients with serologically confirmed West Nile virus (WNV), CNS disease, and their correlation with outcome. Methods: CSF samples from 334 WNV- infected hospitalized patients were analyzed. Information was available and extracted from the medical records of 250 of these patients, and CSF parameters correlated with clinical and epidemiologic features of disease (e.g., patient age, sex, outcome). Results: Patients with meningitis had a mean of 226 cells/mm3,and those with encephalitis had a mean of 227 cells/*** percent of meningitis patients and 5% of encephalitis patients had fewer than 5 cells/mm3, and approximately 8% of both groups had more than 500 cells/mm3. Patients with meningitis had a mean of 41% neutrophils, and those with encephalitis had 45% . Forty- five percent of meningitis patients and 37% of encephalitis patients had at least 50% neutrophils in their initial CSF specimen. Neither the mean percent neutrophils nor their distribution differed significantly between groups. Forty- seven percent of encephalitis patients and 16% of meningitis patients had CSF protein of 100 mg/dL or greater (p < 0.01). Although specific CSF parameters, including nucleated cell count and protein concentration, correlated significantly with outcome, multivariate analysis suggested that their total predictive value was modest. Age was an additional predictor of outcome independent of CSF variables in all patients. Conclusions: Serologically confirmed West Nile virus meningitis and encephalitis produce similar degrees of CSF pleocytosis and are frequently associated with substantial CSF neutrophilia. Patients with encephalitis have higher CSF protein concentrations and are more likely to have adverse outcomes, including admission to long- term care facilities or even death after their acute illness. CSF findings were only a modest predictor of disease outcome, with patient age adding importan

关键词：脑炎 CSF 患者病毒性脑膜炎血清学检查结果尼罗河

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恢复人体的触觉

科技纵览 2016年第5期 26-31页

作者： Dustin j.tyler Mike McGregor

伊格尔·斯派蒂克（IgorSpetic）戴上眼罩和降噪耳机，摸索到他面前的碗里，伸手进去摸到了一个樱桃的茎，并顺势拿起了樱桃。他用的是左手，他自己的手。

关键词：触觉降噪耳机樱桃

煤的快速热分介——褐煤在小型流化床反应器中脱挥发份

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煤炭转化 1982年第1期 25-33页

作者： R.j.tyler 赵振本

用小型流化床热介器研究了磨细的澳大利亚 Loy Yang 褐煤（<0.2毫米）在快速加热条件下的脱挥发份性能。热介器连续运行,煤以1～3克/小时的加料速度直接加入用氮气流化的砂子床内。颗粒的加热速度可能超过10℃/秒。在热介温度范围为435... 详细信息

用小型流化床热介器研究了磨细的澳大利亚 Loy Yang 褐煤（<0.2毫米）在快速加热条件下的脱挥发份性能。热介器连续运行,煤以1～3克/小时的加料速度直接加入用氮气流化的砂子床内。颗粒的加热速度可能超过10℃/秒。在热介温度范围为435～900℃内,记录了焦油、C1—C3碳氢化合物和总挥发物的收率。在580℃得到了23%（重量）的最大焦油收率（以干燥无灰煤为基准）。比 Fisc-her 检定法得到的数量增加60%。C1—C3碳氢化合物的收率随温度升高而增加,在900℃时达到8%。元素分析表明,焦油和半焦组份极大地取决于热介温度。也采用煤中添加水份,用氢气流化和用石油焦代替砂子流化床等方法,研究了煤脱挥发份的效果。

关键词：挥发份碳氢化合物挥发物乙烷挥发分烷烃半焦流化床反应器沸腾床反应器焦油收集器收率褐煤柴煤

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将汽车IC中的闩锁敏感度降至最低

世界电子元器件 2008年第7期 26-28页

作者： j.hn j.Naughton Matthew A tyler 安森美半导体

对于半导体供应商而占,要对汽车应用的新j.设计进行合格验证,通过闩锁免疫性测试是一项挑战。芯片设计人员可能需要在裸片上增加关键晶体管的物理间隔,但实际上,此举不会带来任何明显的好处,在某些情况下甚至还可能产生某些副作用。理... 详细信息

对于半导体供应商而占,要对汽车应用的新j.设计进行合格验证,通过闩锁免疫性测试是一项挑战。芯片设计人员可能需要在裸片上增加关键晶体管的物理间隔,但实际上,此举不会带来任何明显的好处,在某些情况下甚至还可能产生某些副作用。理解其原因以及如何预测闩锁阈值，就可以应用有效的布线技巧和保护结构，满足闩锁免疫目标的最佳实践方法。

关键词：汽车应用闩锁敏感度 IC 半导体供应商设计人员保护结构实践方法

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煤的快速热分解——烟煤在小型流化床反应器中脱挥发份

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煤炭转化 1982年第1期 34-44页

作者： R.j.tyler 祝岳民

采用小型流化床反应器,在快速加热的情况下研究了10种烟煤的脱挥发份性能。热介器系连续运行,煤粒以每小时1—3克的速度,用氮气直接喷入流化的热砂床中。报导了所有煤的焦油收率,C1—C3碳氢化合物气体收率及总挥发产物和粘结指数。约在... 详细信息

采用小型流化床反应器,在快速加热的情况下研究了10种烟煤的脱挥发份性能。热介器系连续运行,煤粒以每小时1—3克的速度,用氮气直接喷入流化的热砂床中。报导了所有煤的焦油收率,C1—C3碳氢化合物气体收率及总挥发产物和粘结指数。约在600℃获得最大的焦油收率,而且总是比葛—全（Gray—King）试验得到的高得多。总的挥发物收率也比工业分析值高得多。最大的焦油收率看来是与煤的 H/C 原子比成正比。焦油的元素分析极大地取决于热介温度。焦油的H/C 原子比与原料煤的 H/C 原子比成正比例。同时也研究了由于改变热介器的气氛和流化床物料的性质对两种煤脱挥发份性能的影响。用氢、氦,二氧化碳或水蒸汽代替氮气,对焦油的收率没有影响,而且（有一个例外）对碳氢化合物气体收率稍有影响。在氢存在下于600℃以上温度时,甲烷的收率增加了。用石油焦代替砂子作流化床物料时,焦油收率大为降低。活性炭流化床实际上不产生焦油。

关键词：热解器挥发物快速热解化合物油收率流化床反应器沸腾床反应器原子比挥发份挥发分烟煤煤炭

Local immunotherapy with interleuk in-2 delivered from biodegradable p olymer microspheres combined with interstitial chemotherapy:a novel treatme nt for experimental malignant glioma

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癌症 2003年第7期22卷 752-752页

作者： Rhines LD DiMeco F Lawson HC tyler BM Hanes j.Olivi A Brem H

OBj.CTIVE:Local delivery of carmustine(BCNU)from biodegradable polymers prolon gs survival against experimental brain ***,paracrine administration of inte rleukin-2(IL-2)has been shown to elicit a potent antitumor immune response and to improve survival in animal brain tumor *** r eport the use of a novel polymeric mic rosphere delivery vehicle to release *** demonstr ate both in vitro release of cytokine from the microspheres and histological evidence of the inflammatory response elicited by I L-2released from the microspheres i n the rat *** microspheres a re used to deliver IL-2,and biodegradable polymer wafers are used to deliver BCNU,directly at the site of an intracranially implanted glioma in the *** two agents administered locally show a s ynergistic ***:Fischer 344rats challenged intracranially with 9L gliosarcoma received an intracranial implant of either empty microspheres or microspheres containing IL-2(IL-2MS ).Five days later,animals in each group were randomized to receiv e polymer implants loaded with 0,3.8,or 10%BCNU at the tumor ***:Animals that received the combination of IL-2MS a nd 3.8%BCNU polymer(median survival,28.5d )or IL-2MS and 10%BCNU polymer(median survival,45.5d )showed significantly improved surv ival compared with animals that rece ived monotherapy with IL-2microsph eres(median survival,24d ),3.8%BCNU polymer(median survival,24d ),or 10%BCNU polymer(median survival,32.5d ).Control animals had a median survival of *** combination of either 3.8or 10%BCNU polymer with IL-2MS resulted in 7and 25%long-term survivors,***:By showing synergy of IL-2and BCNU in an animal glioma model and using a reproducible synthetic delivery sy stem for each agent (i.e.,one that did not rely on genetic ally engineered cells or viruses),we hope that the combination of local immunotherapy and chemotherapy can take an important step closer to clinical applic ation in patients with

关键词：实验性神经胶质瘤 IL-2 腔内化疗

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