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Brocaeloid D,a novel compound isolated from a wheat pathogenic fungus, Microdochium majus 99049

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Synthetic and Systems Biotechnology 2019年第4期4卷 173-179页

作者： Jingyu Zhang Zhenzhen Wang Zhijun Song Loganathan Karthik Chengjian Hou Guoliang Zhu lan.Jiang Jianying Han Rong Ma Li Li Lixin Zhang Xueting Liu tom Hsiang State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai200237China Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing100101China Department of Medicinal Chemistry Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100050China School of Environmental Sciences University of Guelph50 Stone Road EastGuelphOntarioN1G 2W1Canada University of Chinese Academy of Sciences Beijing100049China

Microbes serve as the most important resource for drug *** our screening for bioactive compounds from our natural products library,a pathogenic fungus,Microdochium majus strain 99049,from wheat was selected for further investigation.A new alkaloid named brocaeloid D(1),together with six previously characterized compounds(2–7)were *** 1 belongs to 4-oxoquinoline with C-2 reversed prenylation and a succinimide *** the structures of these newly isolated compounds were determined by different means in spectroscopic *** absolute configurations of 1 was further deduced from comparison of its CD spectrum with that of known compound *** bioactivities of these identified compounds were evaluated against several pathogenic microorganisms and cancer cell *** 1–5 showed activity against HUH-7 human hepatoma cells with IC50 values of 80μg/*** 6 showed mild activity against HeLa cells(IC50=51.9μg/mL),weak anti-MTB activity(MIC=80μg/mL),and moderate anti-MRSA activity(MIC=25μg/mL),and compound 7 showed weak anti-MRSA activity(MIC=100μg/mL).

关键词： Wheat pathogenic fungus Anticancer Antibacterial Natural product

Norditerpenoids biosynthesized by variediene synthase-associated P450 machinery along with modifications by the host cell Aspergillus oryzae

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Synthetic and Systems Biotechnology 2022年第4期7卷 1142-1147页

作者： lan.Jiang Kangjie Lv Guoliang Zhu Zhi Lin Xue Zhang Cuiping Xing Huanting Yang Weiyan Zhang Zhixin Wang Chengwei Liu Xudong Qu tom Hsiang Lixin Zhang Xueting Liu State Key Laboratory of Bioreactor Engineering East China University of Science of TechnologyShanghaiChina State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology Shanghai Jiao Tong UniversityShanghai200240China Key Laboratory of Saline-alkali Vegetation Ecology Restoration Ministry of EducationCollege of Life SciencesNortheast Forestry UniversityHarbinChina School of Environmental Sciences University of Guelph50 Stone Road EastGuelphOntarioCanada

The chemical diversity of terpenoids is typically established by terpene synthase-catalyzed cyclization and diversified by post-tailoring *** bifunctional terpene synthase(BFTS)associated P450 enzymes have shown significant catalytic potentials through the development of various new terpenoids with different biological *** study discovered the BFTS and its related gene cluster from the plan. endophytic fungus Didymosphaeria variabile *** expression of the BFTS in Saccharomyces cerevisiae resulted in the characterization of a major product diterpene variediene(1),along with two new minor products neovariediene and *** heterologous expression of the BFTS and one cytochrome P450 enzyme VndE(CYP6138B1)in Aspergillus oryzae NSAR1 led to the identification of seven norditerpenoids(19 carbons)with a structurally unique 5/5 bicyclic ring ***,in vivo experiments suggested that the cyclized terpene variediene(1)was modified by VndE along with the endogenous enzymes from the host cell *** through serial chemical conversions,followed by multi-site hydroxylation via *** endogenous *** work revealed that the two-enzymes biosynthetic system and host cell machinery could produce structurally unique terpenoids.

关键词： Bifunctional terpene synthases Norditerpenoids Cytochrome P450 Aspergillus oryzae Heterologous expression

Sperm flagellar 2(SPEF2)is essential for sperm flagellar assembly in humans

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Asian Journal of Andrology 2022年第4期24卷 359-366页

作者： Dong-Yan Li Xiao-Xuan Yang Chao-Feng Tu Wei-Li Wang lan.lan.Meng Guang-Xiu Lu Yue-Qiu Tan Qian-Jun Zhang Juan Du Institute of Reproductive and Stem Cell Engineering School of Basic Medical ScienceCentral South UniversityChangsha 410078China Clinical Research Center for Reproduction and Genetics in Hunan Province Reproductive and Genetic Hospital of CITIC-XiangyaChangsha 410078China

Spermiogenesis is a complex and tightly regulated process,consisting of acrosomal biogenesis,condensation of chromatin,flagellar assembly,and disposal of extra *** studies have reported that sperm flagellar 2(SPEF2)deficiency causes severe asthenoteratozoospermia owing to spermiogenesis failure,but the underlying molecular mechanism in humans remains ***,we performed proteomic analysis on spermatozoa from three SPEF2 mutant patients to study the functional role of SPEF2 during sperm tail development.A total of 1262 differentially expressed proteins were detected,including 486 upregulated and 776 *** constructed heat map of the differentially expressed proteins showed similar *** these,the expression of proteins related to flagellar assembly,including SPEF2,sperm associated antigen 6(SPAG6),dynein light chain tctex-type 1(DYNLT1),radial spoke head component 1(RSPH1),translocase of outer mitochondrial membrane 20(tom20),EF-hand domain containing 1(EFHC1),meiosis-specific nuclear structural 1(MNS1)and intraflagellar transport 20(IFT20),was verified by western *** clustering analysis indicated that these differentially expressed proteins were specifically enriched for terms such as spermatid development and flagellar ***,we showed that SPEF2 interacts with radial spoke head component 9(RSPH9)and IFT20 in vitro,which are well-studied components of radial spokes or intra-flagellar transport and are essential for flagellar *** results provide a rich resource for further investigation into the molecular mechanism underlying the role that SPEF2 plays in sperm tail development and could provide a theoretical basis for gene therapy in SPEF2 mutant patients in the future.

关键词： SPEF2 flagellar assembly male infertility protein interaction proteomics

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幽门螺杆菌感染相关胃癌化疗患者症状群特征

中华医院感染学杂志 2024年第2期34卷 200-204页

作者：樊丽媛李梦鸽何义富丁从兰毕清泉安徽医科大学护理学院安徽合肥230601 中国科学技术大学附属第一医院(安徽省立医院)西区肿瘤内科一病区安徽合肥230031

目的探讨幽门螺杆菌(Hp)感染相关胃癌化疗患者症状群的内部特征及其影响因素。方法采用横断面调查,于2021年12月—2022年5月选取中国科学技术大学附属第一医院西区收治的211例胃癌患者为研究对象,收集患者化疗期间疲乏、疼痛、食欲不振... 详细信息

目的探讨幽门螺杆菌(Hp)感染相关胃癌化疗患者症状群的内部特征及其影响因素。方法采用横断面调查,于2021年12月—2022年5月选取中国科学技术大学附属第一医院西区收治的211例胃癌患者为研究对象,收集患者化疗期间疲乏、疼痛、食欲不振、睡眠障碍、焦虑、抑郁症状,使用潜在类别模型分组,多分类Logistic回归分析其影响因素。结果Hp感染相关胃癌化疗患者症状群表现为4种不同的类别,分别命名为“低症状组”、“高症状组”、“低心理症状组”和“高心理症状组”,占比依次为27.4%、19.0%、43.1%和10.4%;多分类Logistic回归分析显示,无吸烟史(OR=0.283,P=0.013)是“低心理症状组”的保护因素,无饮酒史(OR=10.852,P=0.011)是“高心理症状组”的危险因素。结论Hp感染相关胃癌患者化疗期间的症状群存在明显不同的分类特征,需要根据不同类别患者的症状特征,给予个性化管理和干预。

关键词：幽门螺杆菌感染胃癌化疗期症状群潜在类别分析问卷调查

不同烟草种质感染番茄斑驳花叶病毒的细胞病理特征

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植物病理学报 2024年第1期54卷 92-101页

作者：李月月张水英周文鹏张天毅兰平秀谭冠林李凡洛阳师范学院生命科学学院洛阳471934 云南农业大学植物保护学院昆明650201 云南农业大学现代教育技术中心昆明650201 云南省农业科学院生物技术与种质资源研究所昆明650201

番茄斑驳花叶病毒(tomato mottle mosaic virus,tomMV)比同属烟草花叶病毒(tobacco mosaic virus,TMV)和番茄花叶病毒(tomato mosaic virus,tomV)在茄科作物上的侵染性更强,为明确受tomMV侵染后烟草不同表现症状对应的细胞病理学特征。... 详细信息

番茄斑驳花叶病毒(tomato mottle mosaic virus,tomMV)比同属烟草花叶病毒(tobacco mosaic virus,TMV)和番茄花叶病毒(tomato mosaic virus,tomV)在茄科作物上的侵染性更强,为明确受tomMV侵染后烟草不同表现症状对应的细胞病理学特征。本文构建了tomMV的侵染性克隆载体,分别对感染tomMV的不同烟草组织中的病毒粒子及不同表现症状的烟草细胞超微结构进行了观察分析。结果表明tomMV为长度约220~310 nm,直径约16~18 nm的杆状粒子,感染tomMV的珊西烟(Nicotiana tabacum *** nc)种子也可携带病毒。tomMV病毒粒子以分散或整齐分层排列于细胞质或液泡内。感染tomMV的珊西烟和黄花烟(***)细胞均产生多泡体结构、大量次级小囊泡以及髓鞘样结构,线粒体嵴减少或消失。其中,症状表现为花叶、畸形的珊西烟叶片细胞中叶绿体变化较大,有膜破碎、大量液泡进入的现象;线形叶细胞中多泡体结构和次级小囊泡数量更多;而表现为系统性坏死的黄花烟叶片细胞中叶绿体变化较小。本研究tom-MV侵染性克隆的构建和不同烟草被感染后的细胞病变观察为进一步研究tomMV的致病机理以及与寄主的互作等提供了重要条件和依据。

关键词：番茄斑驳花叶病毒侵染性克隆载体细胞病变珊西烟黄花烟

Effects of Extreme Air Temperature and Humidity on the Insecticidal Expression Level of Bt Cotton

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Journal of Integrative Agriculture 2012年第11期11卷 1836-1844页

作者： CHENYuan WEN Yu-jin CHEN Yuan John tom Cothren ZHANG Xiang WANG Yong-hui WilliamA Payne CHEN De-hua Ecology and Cultivation in Middle and Lower Reaches of Yangtse River Ministry of Agriculture/Agricultural College Yangzhou University Yangzhou 225009 P.R.China Department of Soil and Crop Sciences Texas A&M University College Station TX77843 USA Key Laboratory of Crops Genetics and Physiology Jiangsu Province/Agricultural College Yangzhou University Yangzhou 225009 P.R. China Norman Borlaug Institute for International Agriculture Texas A&M University College Station TX77843 USA

The higher survival rates of Helicoverpa amigera larvae were usually observed after adverse climate which was related to extreme temperature （T） and relative humidity （RH） stresses in transgenic Bacillus thuringiensis （Bt） cotton. The unstable resistance of Bt cotton to bollworms has been correlated with the reduced expression of CrylAc δ-endotoxin. The objective of this study was to investigate the effects of combined temperature and relative humidity stresses on the leaf CrylAc insecticidal protein expression during critical developmental stages. The study was undertaken on two transgenic cotton cultivars that share same parental background, Sikang 1 （a conventional cultivar） and Sikang 3 （a hybrid cultivar）, during the 2007 and 2008 growing seasons at the Yangzhou University Farm, Yangzhou, China. The study was arranged with two factors that consisted of temperature （two levels） and relative humidity （three levels）. The six T/RH treatments were 37℃/95%, 37℃/70%, 37℃/50%, 18℃/95%, 18℃/70%, and 18℃/50%. In 2007, the six treatments were imposed to the plan.s at peak flowering stage for 24 h; in 2008, the six treatments were applied to the plan.s at peak square, peak flowering, and peak boll stages for 48 h. The results of the study indicated that the leaf insecticidal protein expression in CrylAc was significantly affected by extreme temperature only at peak flowering stage, and by both extreme temperature and relative humidity during boll filling stage. The greatest reductions were observed when the stresses were applied at peak boll stage. In 2008, after 48 h stress treatment, the leaf Bt endotoxin expression reduced by 25.9-36.7 and 23.6-40.5% at peak boll stage, but only by 14.9-26.5 and 12.8-24.0% at peak flowering stage for Sikang 1 and Sikang 3, respectively. The greatest reduction was found under the low temperature combined with low relative humidity condition for both years. It is believed that the temperature and relative humidity stresses may be attribut

关键词： Bt cotton high temperature low temperature relative humidity CryIAc protein

急性下呼吸道感染的抗生素处方策略及用药说明——随机对照试验

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美国医学会杂志（中文版） 2006年第4期25卷 203-209页

作者： Paul Little Kate Rumsby Joanne Kelly Louise Watson Michael Moore Gregory Warner tom Fahey lan.Williamson 马凌云（译）文仲光（校） Primary Medical Care Group University of Southampton Highfield Nightingale Surgery Romsey Hants Three Swans Surgery Salisbury England Department of Primary Carel Dundee University Dundee Scotland 不详

背景：在初级医疗机构治疗的疾病中，急性下呼吸道感染最为常见。尽管有关抗生素应用的系统综述很少，且得出的结论各有不同，但仍有许多内科医生开处抗生素。目的：评价急性下呼吸道感染的三种抗生素处方策略和一种用药说明的有效性。... 详细信息

背景：在初级医疗机构治疗的疾病中，急性下呼吸道感染最为常见。尽管有关抗生素应用的系统综述很少，且得出的结论各有不同，但仍有许多内科医生开处抗生素。目的：评价急性下呼吸道感染的三种抗生素处方策略和一种用药说明的有效性。设计、地点及患者：于1998年8月18日至2003年7月30日进行随机对照试验，807例病人均因单纯急性下呼吸道感染而于初级保健机构就诊。按析因设计将病人分为6组：有用药说明书或无用药说明书与3个不同抗生素使用组（立即使用抗生素、不使用抗生素、延后使用抗生素）的任一组合。干预：三种处方策略包括立即使用抗生素（n=262）、延后使用抗生素（n=272）与不使用抗生素（n=273）。每组大约一半病人给予用药说明书（立即使用抗生素组129例，延后使用抗生素组136例，不使用抗生素组140例）。主要观测指标：症状持续时间及严重程度。结果：总共562例（70％）病人交回了完整的日记，78例（10％）病人提供了症状持续时间及严重程度的信息。分级至少为“一个小问题”的咳嗽平均持续11．7天（25％的病人咳嗽持续≥17天）。用药说明书对主要结果没有影响。与不使用抗生素组相比，其他策略并未使咳嗽持续时间（延后使用抗生素组：0．75天；95％可信区间[CI]，-0．37～1．88；立即使用抗生素组：0．11天；95％CI，-1．01～1．24）或其他主要结局发生改变。与立即使用抗生素组相比，延后使用抗生素组和对照组较少使用抗生素（分别为96％、20％和16％，P〈0．001），少数病人感到非常满意（分别为86％、77％和72％，P=0．005），少数病人相信抗生素的效果（分别是75％、40％和47％，P〈0．001）。使用抗生素组的病人1个月内复诊者较少（不使用抗生素组、延后使用抗生素组和立即使用抗生素组分别为0．19、0．12和0．11，P=0．04）；有用药说明书的病人就诊较多（无用药说明书和有用药说明书者分别为0．11和0．17，P=0．02）。结论：单纯急性下呼吸道感染者不用抗生素或延后使用抗生素是可以接受的，症状的消失在各组间差别很小，应明显减少抗生素的使用，消除对抗生素效果的迷信。

关键词：急性下呼吸道感染抗生素应用随机对照试验用药处方初级医疗机构初级保健机构持续时间析因设计严重程度

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心理理论研究的起源与进展

湖北大学学报（哲学社会科学版） 2005年第5期32卷 578-582页

作者：罗杰卿素兰湖北大学教育学院湖北武汉430062 中央教育科学研究所北京100088

自1983年Wimmer和Perner创设了心理理论研究的经典实验范式——“错误信念任务”范式以来,心理理论的研究成为发展心理学研究中的一个热点课题。文章主要对以下几个方面的研究进行了综述:心理理论研究的起源、研究范式、Wellman等人对... 详细信息

自1983年Wimmer和Perner创设了心理理论研究的经典实验范式——“错误信念任务”范式以来,心理理论的研究成为发展心理学研究中的一个热点课题。文章主要对以下几个方面的研究进行了综述:心理理论研究的起源、研究范式、Wellman等人对儿童心理理论研究的元分析过程和结果、心理理论发展的影响因素和研究范围,并在此基础上讨论了以往研究的不足以及今后研究的方向。

关键词：心理理论错误信念元分析

Micro-tom番茄矮化微型机制及其在植物功能基因组学研究中的应用

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遗传 2008年第10期30卷 1257-1264页

作者：刘小花张岚岚朱长青陈昆松徐昌杰浙江大学农业与生物技术学院园艺系杭州310029

Micro-tom番茄植株矮小,生长密度高,生命周期短,容易被高效转化,成为功能基因组学研究的新型模式植物。文章对Micro-tom的由来和生物学特征作了概述,重点阐述了Micro-tom矮化微型的遗传机制以及该番茄在功能基因组学研究中的应用和遗传... 详细信息

Micro-tom番茄植株矮小,生长密度高,生命周期短,容易被高效转化,成为功能基因组学研究的新型模式植物。文章对Micro-tom的由来和生物学特征作了概述,重点阐述了Micro-tom矮化微型的遗传机制以及该番茄在功能基因组学研究中的应用和遗传转化体系研究的进展。

关键词： Micro-tom番茄矮化微型功能基因组学遗传转化