Norditerpenoids biosynthesized by variediene synthase-associated P450 machinery along with modifications by the host cell Aspergillus oryzae

作     者：Lan Jiang Kangjie Lv Guoliang Zhu Zhi Lin Xue Zhang Cuiping Xing Huanting Yang Weiyan Zhang Zhixin Wang Chengwei Liu Xudong Qu Tom Hsiang Lixin Zhang Xueting Liu 

作者机构：State Key Laboratory of Bioreactor EngineeringEast China University of Science of TechnologyShanghaiChina State Key Laboratory of Microbial Metabolism and School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai200240China Key Laboratory of Saline-alkali Vegetation Ecology RestorationMinistry of EducationCollege of Life SciencesNortheast Forestry UniversityHarbinChina School of Environmental SciencesUniversity of Guelph50 Stone Road EastGuelphOntarioCanada 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2022年第7卷第4期

页      面：1142-1147页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：the financial support from the National Key Research and Development Program of China(2020YFA0907800 and 2019YFA0906200) the National Natural Science Foundation of China(21907031,81903529,21977029,31720103901,21877124) the Open Project Funding of the State Key Laboratory of Bioreactor Engineering the 111 Project(B18022) Genome sequencing and assembly of strain DV17020 were supported by funding from the Natural Science and Engineering Research Council of Canada to Prof.T.Hsiang 

主　　题：Bifunctional terpene synthases Norditerpenoids Cytochrome P450 Aspergillus oryzae Heterologous expression 

摘      要：The chemical diversity of terpenoids is typically established by terpene synthase-catalyzed cyclization and diversified by post-tailoring *** bifunctional terpene synthase(BFTS)associated P450 enzymes have shown significant catalytic potentials through the development of various new terpenoids with different biological *** study discovered the BFTS and its related gene cluster from the plant endophytic fungus Didymosphaeria variabile *** expression of the BFTS in Saccharomyces cerevisiae resulted in the characterization of a major product diterpene variediene(1),along with two new minor products neovariediene and *** heterologous expression of the BFTS and one cytochrome P450 enzyme VndE(CYP6138B1)in Aspergillus oryzae NSAR1 led to the identification of seven norditerpenoids(19 carbons)with a structurally unique 5/5 bicyclic ring ***,in vivo experiments suggested that the cyclized terpene variediene(1)was modified by VndE along with the endogenous enzymes from the host cell *** through serial chemical conversions,followed by multi-site hydroxylation via *** endogenous *** work revealed that the two-enzymes biosynthetic system and host cell machinery could produce structurally unique terpenoids.