检索结果-南通市图书馆

Testicular oxidative stress and apoptosis status in streptozotocin-induced diabetic rats after treatment with rooibos(Aspalathus linearis),honeybush(Cyclopia intermedia),and sutherlandia(Lessertia frutescens)infusions

引用

Asian pacific Journal of Reproduction 2021年第1期10卷 11-20页

作者： Temidayo s.Omolaoye shantal Windvogel stefan s.du plessis Division of Medical Physiology Faculty of Medicine and Health SciencesStellenbosch UniversityTygerbergSouth Africa Department of Basic sciences College of MedicineMohammed Bin Rashid University of Medicine and Health SciencesDubaiUnited Arab Emirates Centre for Cardio-metabolic Research in Africa Stellenbosch UniversitySouth Africa

Objective:To investigate the testicular oxidative stress and apoptosis status,as well as the sperm functional parameters in streptozotocin(sTZ)induced diabetic rats following treatment with rooibos(Aspalathus linearis),honeybush(Cyclopia intermedia)and sutherlandia(Lessertia frutescens)***:Diabetes was induced by injecting fourteen-week-old adult male Wistar rats(250-300 g)with a single intraperitoneal injection of sTZ(45 mg/kg body weight).Fifty rats were randomly divided into five groups:the vehicle group received 0.1 M citrate buffer,the diabetic control group received 45 mg/kg sTZ,the diabetic+rooibos group received 45 mg/kg sTZ+2.0%rooibos,the diabetic+honeybush group received 45 mg/kg sTZ+4.0%honeybush,and the diabetic+sutherlandia group received 45 mg/kg sTZ+0.2%*** were sacrificed 7 weeks after induction of diabetes *** testes and epididymides were harvested and weighed after *** were retrieved from the cauda epididymis for motility,concentration,and morphology analysis,and the testis was used for all biochemical *** stress was determined by measuring malondialdehyde levels,catalase,and superoxide dismutase activities,while apoptotic biomarkers were evaluated by Western blotting ***:After induction of diabetes,rats in the diabetic control group,diabetic+rooibos group,diabetic+honeybush group,and diabetic+sutherlandia group presented with significantly elevated blood glucose levels as compared with the vehicle group(Ps in the diabetic control group had a reduction in sperm progressive motility,while rats in the diabetic+rooibos group and the diabetic+sutherlandia group displayed an increase in progressive motility as compared with the diabetic control *** diabetic control animals showed a 40.0%decrease in sperm concentration when compared to the vehicle group,and there were no significant differences in sperm kinematic and speed parameters between the *** add

关键词： Diabetes sperm Oxidative stress Apoptosis Rooibos Honeybush sutherlandia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insulin and leptin enhance human sperm motility, acrosome reaction and nitric oxide production

引用

Asian Journal of Andrology 2008年第5期10卷 799-807页

作者： Fanuel Lampiao stefan s. du plessis Division of Medical Physiology Department of Biomedical Sciences University of Stellenbosch Tygerberg 7505 South Africa

Aim： To investigate the in vitro effects of insulin and leptin on human sperm motility, viability, acrosome reaction and nitric oxide （NO） production. Methods： Washed human spermatozoa from normozoospermic donors were treated with insulin （10 μIU） and leptin （10 nmol）. Insulin and leptin effects were blocked by inhibition of their intracellular effector, phosphotidylinositol 3-kinase （PI3K）, by wortmannin （10 μmol） 30 min prior to insulin and leptin being given. Computer-assisted semen analysis was used to assess motility after 1, 2 and 3 h of incubation. Viability was assessed by fluorescence-activated cell sorting using propidium iodide as a fluorescent probe. Acrosome-reacted cells were observed under a fluorescent microscope using fluorescein-isothiocyanate-Pisum sativum agglutinin as a probe. NO was measured after treating the sperm with 4,5-diaminofluorescein-2/diacetate （DAF-2/DA） and analyzed by fluorescence-activated cell sorting. Results： Insulin and leptin significantly increased total motility, progressive motility and acrosome reaction, as well as NO production. Conclusion： This study showed the in vitro beneficial effects of insulin and leptin on human sperm function. These hormones could play a role in enhancing the fertilization capacity of human spermatozoa.

关键词： insulin leptin spermatozoa nitric oxide motility acrosome reaction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

3xidative phosphorylation versus glycolysis： what Fuel do spermatozoa use？

引用

Asian Journal of Andrology 2015年第2期17卷 230-235,I0007,I0008页

作者： stefan s du plessis Ashok Agarwal Gayatri Mohanty Michelle van der Linde Division of Medical Physiology Department of Biomedical Sciences Faculty of Medicine and Health Sciences Stellenbosch University Tygerberg South Africa Center for Reproductive Medicine Cleveland Clinic Cleveland Ohio USA Department of Zoology Ravenshaw University Cuttack Odisha India

spermatozoa are highly specialized cells. Adenosine triphosphate （ATP）, which provides the energy for supporting the key functions of the spermatozoa, is formed by 2 metabolic pathways, namely glycolysis and oxidative phosphorylation （OXPHOs）. It is produced in the mitochondria through OXPHOs as well as in the head and principal piece of the flagellum through glycolysis. However, there is a great discrepancy as to which method of ATP production is primarily utilized by the spermatozoa for successful fertilization. Mitochondrial respiration is considered to be a more efficient metabolic process for ATP synthesis in comparison to glycolysis. However, studies have shown that the diffusion potential of ATP from the mitochondria to the distal end of the flagellum is not sufficient to support sperm motility, suggesting that glycolysis in the tail region is the preferred pathway for energy production. It is suggested by many investigators that although glycolysis forms the major source of ATP along the flagellum, energy required for sperm motility is mainly produced during mitochondrial respiration. Nevertheless, some studies have shown that when glycolysis is inhibited, proper functioning and motility of spermatozoa remains intact although it is unclear whether such motility can be sustained for prolonged periods of time, or is sufficiently vigorous to achieve optimal fertilization. The purpose of this article is to provide an overview of mammalian sperm energy metabolism and identify the preferred metabolic pathway for ATP generation which forms the basis of energy Droduction in human spermatozoa during fertilization.

关键词： adenosine triphosphate energy glycolysis oxidative phosphorylation spermatozoa

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Descriptive histomorphological evaluation of the testis and caudal epididymis following treatment with rooibos(Aspalathus linearis),honeybush(Cyclopia intermedia)and sutherlandia(Lessertia frutescens)in healthy and streptozotocin-induced diabetic rats

引用

Asian pacific Journal of Reproduction 2021年第4期10卷 176-186,I0001,I0002页

作者： Temidayo s.Omolaoye stefan s.du P.lessis Department of Basic sciences College of MedicineMohammed Bin Rashid University of Medicine and Health SciencesDubaiUnited Arab Emirates Division of Medical Physiology Faculty of Medicine and Health SciencesStellenbosch UniversityTygerbergSouth Africa

Objective:To evaluate the testis and the cauda epididymis after treating both healthy and diabetic rats with rooibos(Aspalathus linearis),honeybush(Cyclopia intermedia),and sutherlandia(Lessertia frutescens),***:Ninety male Wistar rats were randomly divided into nine groups(n=10 per group).Fifty rats were randomized to receive tap water,0.1 M citrate buffer,2.0%fermented rooibos,4.0%fermented honeybush,or 0.2%unfermented *** was induced via a single intraperitoneal injection of streptozotocin(sTZ)at 45 mg/kg in 40 *** diabetic rats were randomized to receive tap water,2.0%fermented rooibos,4.0%fermented honeybush,or 0.2%unfermented *** were sacrificed after seven weeks of diabetic *** was collected for hormonal assay,while the testes and caudal epididymis were retrieved for histomorphological ***:Diabetic rats treated with 2.0%fermented rooibos exhibited a significant increase in fasting plasma glucose(FPG)versus the vehicle group(P=0.002),and a 3.6%decrease compared to the diabetic control group(P>0.05).Diabetic rats treated with honeybush or sutherlandia showed 23.6%and 15.4%decrease in FPG when compared to the diabetic control group,respectively(both P>0.05).The diabetic control rats had a significant decrease in the percentage of histologically normal seminiferous tubules compared to the control group(P=0.049).There was 21.0%,14.0%,and 5.4%rise in the percentage of normal seminiferous tubules in diabetic rats receiving rooibos,honeybush or sutherlandia,respectively,compared to the diabetic control *** infusion control groups(rooibos,honeybush and sutherlandia)showed normal seminiferous tubules,presence of spermatozoa in the epididymal lumen,and had normal overall *** testicular and epididymal morphology were altered in the diabetic control group,but these disruptions were mildly ameliorated by rooibos,honeybush,and ***:The detrimental effect

关键词： Testis Epididymis Rooibos Honeybush sutherlandia Diabetes Histomorphological evaluation streptozotocin.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

New developments of the effect of melatonin on reproduction

引用

World Journal of Obstetrics and Gynecology 2013年第2期2卷 8-15页

作者： Fanuel Lampiao stefan s du plessis Division of Physiology Department of Basic Medical SciencesCollege of MedicineBlantyre 3Malawi Division of Medical Physiology Stellenbosch UniversityTygerberg CampusTygerberg 19063South Africa

In the past decades, a lot of advances in understanding the biochemistry and physiology of the pineal gland have been made. There is evidence that it interacts with many endocrine as well as non-endocrine tissues to influence their metabolic activity modulating many organs and functions. Melatonin is secreted by the pineal gland in the brain and plays an important role in regulating the neuroendocrine system. This hormone is one of the major role players in the regulation of the circadian sleep-wake cycle. It is normally released from the pineal gland during the night in response to environmental changes in light. studies have shown that melatonin plays a role in the regulation of many reproductive processes such as puberty, gonadal function, and pregnancy. Beside these, melatonin has been shown to be able to directly neutralize a number of free radicals and reactive oxygen and nitrogen species. The main objective of this review is to provide comprehensive information about the new developments in melatonin research regarding its role in reproduction. A review of international scientific literature was done and a question-and-answer format was used in an attempt to convey comprehensive information in a simple manner. This review discusses evidence currently available relating to the effect of melatonin on reproductive processes. It deliberates the mechanism of action of melatonin, its effect on puberty, testicular and ovafunction, pregnancy, and oxidative stress. A growing body of scientific evidence is suggesting that melatonin plays an important role in reproductive function. It is therefore imperative to highlight the beneficial effects of this hormone in improving the reproductive processes.

关键词： Melatonin Reproduction Reactive oxygen species Antioxidants Pineal gland

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Obesity： modern man＇s fertility nemesis

引用

Asian Journal of Andrology 2010年第4期12卷 480-489页

作者： stephanie Cabler Ashok Agarwal Margot Flint stefan s. du plessis Center for Reproductive Medicine The Cleveland Clinic 9500 Euclid Avenue Cleveland OH 44195 USA Division of Medical Physiology Faculty of Health Sciences Stellenbosch University PO Box 19063 Tygerberg 7505 South Africa

The obesity pandemic has grown to concerning proportions in recent years, not only in the Western World, but in developing countries as well. The corresponding decrease in male fertility and fecundity may be explained in parallel to obesity, and obesity should be considered as an etiology of male fertility. studies show that obesity contributes to infertility by reducing semen quality, changing sperm proteomes, contributing to erectile dysfunction, and inducing other physical problems related to obesity. Mechanisms for explaining the effect of obesity on male infertility include abnormal reproductive hormone levels, an increased release of adipose-derived hormones and adipokines associated with obesity, and other physical problems including sleep apnea and increased scrotal temperatures. Recently, genetic factors and markers for an obesity-related infertility have been discovered and may explain the difference between fertile obese and infertile obese men. Treatments are available for not only infertility related to obesity, but also as a treatment for the other comorbidities arising from obesity. Natural weight loss, as well as bariatric surgery are options for obese patients and have shown promising results in restoring fertility and normal hormonal profiles. Therapeutic interventions including aromatase inhibitors, exogenous testosterone replacement therapy and maintenance and regulation of adipose-derived hormones, particularly leptin, may also be able to restore fertility in obese males. Because of the relative unawareness and lack of research in this area, controlled studies should be undertaken and more focus should be given to obesity as an etiolgy of male infertility.

关键词： adipokines infertility leptin obesity spermatozoa

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Diabetes mellitus and male infertility

引用

Asian pacific Journal of Reproduction 2018年第1期7卷 6-14页

作者： Omolaoye Temidayo s du plessis stefan s Division of Medical Physiology Faculty of Medicine and Health SciencesStellenbosch UniversityTygerbergSouth Africa

Infertility is prevalent in about 10%-25% of couples in their reproductive age, analogous to 60-80 million infertile couples globally. Of these infertility cases, 10%-30% are exclusively attributed to a problem of the male. several diseases have been implicated as contributors to deteriorating male fertility and diabetes mellitus (DM) is included. DM, a chronic non-communicable disease, has been considered as one of the most appreciable health threats, as it affects 9% (422 million) of the world's population as of 2014. It is characterised by hyperglycaemia, which can result from the inability of the pancreaticβ-cells to secrete insulin or from the target tissue becoming insensitive to insulin. DM has been reported to influence male reproductive function through diverse pathways and mechanisms. The adverse effects of reactive oxygen species and successive development of oxidative stress that occur due to DM have been investigated and implicated by several studies. The products of non-enzymatic glycosylation are reported to be widely distributed in the reproductive tract of diabetic men. Additionally, DM has been implicated to impair the processes of male sexual acts. Data reported in this review were extracted from PubMed, Google scholar, science Direct and scopus with diabetes and male infertility as the key search words. In light of the aforementioned, the aim of this review is to provide brief background information on DM as well highlight and explain the likely mechanisms of male fertility which DM impacts.

关键词： Diabetes mellitus Male infertility Diabetic neuropathy Oxidative stress Reactive oxygen species Advanced glycated end products

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The in vitro effects of superoxide, some commercially available antioxidants and red palm oil on sperm motility

引用

Asian Journal of Andrology 2009年第6期11卷 695-702页

作者： Yapo Guillaume Aboua stefan stephanus du plessis Patricia Reichgelt Nicole Brooks Department of Biomedical sciences Faculty of Health and Wellness Sciences Cape Peninsula University of TechnologyBellville 7535 South Africa Division of Medical Physiology Faculty of Health Sciences Stellenbosch University Tygerberg 7505 South Africa

In this study, two commercially available superoxide scavengers, tetrakis （1-methyl-4-pyridyl） porphyrin （Mn[III]TMPyP） and superoxide dismutase （sOD）, as well as red palm oil （RPO）, a natural vegetable oil, had been used to investigate their possible in vitro effects against the toxic effects of superoxide （O2＋） on human sperm motility. semen samples were obtained from 12 normozoospermic healthy volunteer donors aged between 19 and 23 years. The O2＋donor 2,3-dimetoxyl-l,4-naphthoquinone （DMNQ）（2.5 μmol· L^-1-100 μmol· L^-1） was added to normozoospermic post-swim-up sperm in the presence or absence of Mn（III）TMPyP （50 μmol· L^-1）, sOD （50 IU） or RPO （0.1% or 0.5%）. Computer-assisted semen analysis was used to analyze various motility parameters. The parameters of interest were percentage of motile cells, progressive motility, rapid cells and static cells. Concentrations of higher than 25 μmol· L^-1 DMNQ were detrimental to sperm motility. Mn（III）TMPyP was able to attenuate the effect of O2＋ on the motility parameters. In vitro addition of sOD and RPO showed harmful effects on sperm motility.

关键词： antioxidants motility red palm oil superoxide

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：