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A chorismate mutase from Radopholus similis plays an essential role in pathogenicity

Journal of Integrative Agriculture 2024年第3期23卷 923-937页

作者： sihua yang Junyi Li Shuai yang Shiqiao Tang Huizhong Wang Chunling Xu Hui Xie Laboratory of Plant Nematology andResearch Centerof Nematodes of Plant Quarantine Department of Plant Patholo of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China

In the process of infecting plants, plant parasitic nematodes release a series of proteins that play an essential role in the successful infection and pathogenesis of plant cells and tissues through stylets or body walls. In this study,based on transcriptome data, a chorismate mutase gene of Radopholus similis(RsCM) was identified and cloned,which is a single copy gene specifically expressed in the oesophageal gland and highly expressed in juveniles and females. Transient expression of RsCM in tobacco leaves showed that it was localised in the cytoplasm and nucleus of tobacco leaf cells, which inhibited the pattern-triggered immunity(PTI) induced by flg22, including callose deposition and defence gene expression, and cell death induced by immune elicitors BAX, but could not inhibit cell death induced by immune elicitors Gpa2/RBP-1. The RNA interference(RNAi) transgenic tomato of RsCM obviously inhibited the infection, pathogenicity, and reproduction of R. similis. However, the resistance of the overexpression transgenic tomato of RsCM to R. similis infection was significantly reduced, and the expression levels of two salicylic acid(SA) pathway genes(PR1 and PR5) in roots infected by the nematode were significantly down-regulated,which indicated that RsCM might be involved in the inhibition of SA pathway. The results of this study demonstrate that RsCM suppresses the host immune system and might be a new target for the control of R. similis, which also provides new data for the function and mechanism of CM genes of migratory parasitic plant nematodes.

关键词： Radopholus similis chorismate mutase plant defense transgenic tomato

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Advancing insights into in vivo meningeal lymphatic vessels with stereoscopic wide-field photoacoustic microscopy

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Light(Science & Applications) 2024年第5期13卷 894-907页

作者： Fei yang Zhiyang Wang Wenbin Shi Miao Wang Rui Ma Wuyu Zhang Xipeng Li Erqi Wang Wenjie Xie Zhan Zhang Qi Shen Feifan Zhou sihua yang MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science South China Normal UniversityGuangzhou 510631China. Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China. Key Laboratory of Biomedical Engineering of Hainan Province School of Biomedical EngineeringHainan UniversityHaikou 570100China. Department of Neurology Sun Yatsen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China. Guangdong Province Key Laboratory of Brain Function and Disease Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510120China. Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter South China Normal UniversityGuangzhou 510006China

Meningeal lymphatic vessels(mLVs)play a pivotal role in regulating metabolic waste from cerebrospinal fluid(CSF).However,the current limitations in field of view and resolution of existing imaging techniques impede understanding the stereoscopic morphology and dynamic behavior of mLVs in ***,we utilized dual-contrast functional photoacoustic microscopy to achieve wide-field intravital imaging of the lymphatic system,including mLVs and glymphatic *** stereoscopic photoacoustic microscopy based on opto-acoustic confocal features has a depth imaging capability of 3.75 mm,facilitating differentiation between mLVs on the meninges and glymphatic pathways within the brain ***,using this imaging technique,we were able to visualize the dynamic drainage of mLVs and identify a peak drainage period occurring around 20–40 min after injection,along with determining the flow direction from CSF to lymph ***,in the Alzheimer’s disease(AD)mouse model,we observed that AD mice exhibit a~70%reduction in drainage volume of mLVs compared to wild-type *** the development of AD,there is be continued decline in mLVs drainage *** finding clearly demonstrates that the AD mouse model has impaired CSF *** study opens up a horizon for understanding the brain’s drainage mechanism and dissecting mLVs-associated neurological disorders.

关键词： lymphatic impaired field

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New insight into photoacoustic conversion efficiency by plasmon-mediated nanocavitation： Implications for precision theranostics

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Nano Research 2017年第8期10卷 2800-2809页

作者： Yujiao Shi sihua yang Da Xing Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science College of Biophotonics South China Normal University Guangzhou 510631 China

The probe-assisted integration of imaging and therapy into a single modality offers significant advantages in bio-applications. As a newly developed photoacoustic （PA） mechanism, plasmon-mediated nanocavitation, whereby photons are effectively converted into PA shockwaves, has excellent advantages for image-guided therapy. In this study, by simulating the laser absorption, temperature field, and nanobubble dynamics using both finite-element analysis and computational fluid dynamics, we quantified the cavitation-induced PA conversion efficiency of a water-immersed gold nanosphere, revealing new insights. Interestingly, sequential multi-bubble emission accompanied by high PA signal production occur under a single high-dose pulse of laser irradiation, enabling a cavitation-induced PA conversion efficiency up to 2%, which is -50 times higher than that due to thermal expansion. The cavitation-induced PA signal has unique frequency characteristics, which may be useful for a new approach for in vivo nanoparticle tracking. Our work offers theoretical guidance for accurate diagnosis and controllable therapy based on plasmon-mediated nanocavitation.

关键词： photoacoustic imaging conversion efficiency nanocavitation theranostics

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Thermally confined shell coating amplifies the photoacoustic conversion efficiency of nanoprobes

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Nano Research 2016年第12期9卷 3644-3655页

作者： Yujiao Shi Huan Qin sihua yang Da Xing Ministry of Education Key Laboratory of Laser Life Science and Institute of Laser Life Science College of Biophotonics South China Normal University Guangzhou 510631 China

Efficient probes/contrast agents are highly desirable for good-performance photoacoustic （PA） imaging, where the PA signal amplitude of a probe is dominated by both its optical absorption and the conversion efficiency from absorbed laser energy to acoustic waves. Nanoprobes have a unique micro- mechanism of PA energy conversion due to the size effect, which, however, has not been quantitatively demonstrated and effectively utilized. Here, we present quantitative simulations of the PA signal production process for plasmon- mediated nanoprobes based on the finite element analysis method, which were performed to provide a deep understanding of their PA conversion micromechanism. Moreover, we propose a method to amplify the PA conversion efficiency of nanoprobes through the use of thermally confined shell coating, which allows the active control of the conversion efficiency beyond that of conventional probes. Additionally, we deduced the dependence of the conversion efficiency on the shell properties. Gold-nanoparticles/polydimethylsiloxane nanocomposites were experimentally synthesized in the form of gel and microfilms to verify our idea and the simulation results agreed with the experiments. Our work paves the way for the rational design and optimization of nanoprobes with improved conversion efficiency.

关键词： photoacousticnanoprobes micromechanism conversion efficiency shell coating

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Editorial—Introduction to the special issue on photoacoustic and microwave-thermoacoustic imaging

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Journal of Innovative Optical Health Sciences 2017年第4期10卷 1-3页

作者： Da Xing sihua yang MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science College of Biophotonics South China Normal University Guangzhou 510631 China

Photoacoustic imaging and microwave-thermoacoustic imaging are innovative hybrid imaging techniques that have experienced rapid development in recent *** imaging is based on the photoacoustic e®*** the laser pulses(the width of the laser pulse is usually several nanoseconds to tens of nanoseconds)irradiate the biological tissue,the absorbers in the tissue absorb the optical energy and then induce the instantaneous rise in temperature,and radiate the thermal energy in the form of mechanical energy,*** *** can detect the ultrasound signals with ultrasound transducer and recover the absorption information of the absorbers in the tissue with di®erent imaging *** imaging integrates the merit of high contrast of optical imaging and high imaging depth of ultrasound *** the excitation source of the photoacoustic imaging is changed into the microwave(the width of the microwave pulse is usually from tens of nanoseconds to hundreds of nanoseconds),that is called thermoacoustic imaging,which can provide high-resolution imaging and imaging depth of more than ten ***,photoacoustic imaging and thermoacoustic imaging have high molecular speci¯city and have already been widely used in the research of physics,chemistry,and biomedicine.

关键词： microwave pulse optical

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“医工融合转化”专题前言

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中国激光 2024年第9期51卷 1-2,F0002页

作者：刘谦黄国亮丁志华邱海霞杨思华海南大学清华大学生物医学工程学院浙江大学二级中国光学学会生物医学光子学专业委员会中国感光学会光学传感与诊断专业委员会中国人民解放军总医院第一医学中心激光医学科华南师范大学华南师范大学激光生命科学教育部重点实验室华南师范大学生物光子学研究院

医工融合转化旨在面向人民生命健康,围绕预防、诊断、治疗和康复等具体需求,通过结合医学知识与工程技术,创造能够满足临床需求的创新医疗产品。医工融合转化不仅促进了医学与工程学领域的深度交叉和协同发展,而且推动了新型医学诊疗技... 详细信息

医工融合转化旨在面向人民生命健康,围绕预防、诊断、治疗和康复等具体需求,通过结合医学知识与工程技术,创造能够满足临床需求的创新医疗产品。医工融合转化不仅促进了医学与工程学领域的深度交叉和协同发展,而且推动了新型医学诊疗技术的研发。此外,它还加速了智能医疗与健康管理的创新,从而显著提升了医疗服务水平,是实现国家“十四五”发展规划中全民健康信息化建设战略目标的重要途径。

关键词：人民生命健康医疗服务水平工程技术全民健康协同发展临床需求智能医疗医学诊疗技术

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Spatial weight matrix in dimensionality reduction reconstruction for microelectromechanical system-based photoacoustic microscopy

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Visual Computing for Industry,Biomedicine,and Art 2020年第1期3卷 247-256页

作者： Yuanzheng Ma Chang Lu Kedi Xiong Wuyu Zhang sihua yang MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

A micro-electromechanical system(MEMS)scanning mirror accelerates the raster scanning of optical-resolution photoacoustic microscopy(OR-PAM).However,the nonlinear tilt angular-voltage characteristic of a MEMS mirror introduces distortion into the maximum back-projection ***,the size of the airy disk,ultrasonic sensor properties,and thermal effects decrease the ***,in this study,we proposed a spatial weight matrix(SWM)with a dimensionality reduction for image *** three-layer SWM contains the invariable information of the system,which includes a spatial dependent distortion correction and 3D *** employed an ordinal-valued Markov random field and the Harris Stephen algorithm,as well as a modified delay-and-sum method during a time *** results from the experiments and a quantitative analysis demonstrate that images can be effectively reconstructed using an SWM;this is also true for severely distorted *** index of the mutual information between the reference images and registered images was 70.33 times higher than the initial index,on ***,the peak signal-to-noise ratio was increased by 17.08%after 3D *** accomplishment offers a practical approach to image reconstruction and a promising method to achieve a real-time distortion correction for MEMS-based OR-PAM.

关键词： Photoacoustic microscopy Spatial weight matrix Dimensionality reduction Distortion correction Mutual information

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Imaging of human wrist joint by a flexible-transducer-based morphological-adaptive photoacoustic tomography: a feasibility study

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Chinese Optics Letters 2019年第9期17卷 73-77页

作者： Xin Wang sihua yang M0E Key Laboratory of Laser Life Science & Institute of Laser Life Science South China Normal UniversityGuangzhou 510631China College of Biophotonics South China Normal UniversityGuangzhou 510631China

In this study, the feasibility of visualization of human joints using photoacoustic tomography(PAT) is investigated. To verify this idea, the system of integrated optical fiber bundles and a custom-made flexible transducer is established, both of which give the advantage of morphological adaptation;therefore, the coupling section can be worn on human limbs. The imaging capacity of the flexible-transducer-based PAT system is validated by mapping the structures of the finger and the wrist joint. To the best of our knowledge, it is the first time to achieve photoacoustic imaging of such large human wrist joints. The cross-sectional photoacoustic images of a healthy joint clearly exhibit the main internal structures, including the phalanx, tendons, and blood vessels, which are comparable with the corresponding images by 3.0 T magnetic resonance *** experimental results demonstrate that the proposed system holds promise for early diagnosis of joint disorders.

关键词： Imaging human wrist joint photoacoustic tomography(PAT)

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In vivo volumetric monitoring of revascularization of traumatized skin using extended depth-of-field photoacoustic microscopy

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Frontiers of Optoelectronics 2020年第4期13卷 307-317页

作者： Zhongwen CHENG Haigang MA Zhiyang WANG sihua yang MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

Faster and better wound healing is a critical medical *** the repair process of wounds is closely related to revascularization,accurate early assessment and postoperative monitoring are very important for establishing an optimal treatment ***,we present an extended depth-of-field photoacoustic microscopy system(E-DOF-PAM)that can achieve a constant spatial resolution and relatively uniform excitation efficiency over a long axial *** superior performance of the system was verified by phantom and in vivo ***,the system was applied to the imaging of normal and trauma sites of volunteers,and the experimental results accurately revealed the morphological differences between the normal and traumatized skin of the epidermis and *** results demonstrated that the E-DOF-PAM is a powerful tool for observing and understanding the pathophysiology of cutaneous wound healing.

关键词： photoacoustic microscopy(PAM) extended depth-of-field traumatized skin

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Tomography-assisted Doppler photoacoustic microscopy:proof of concept

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Chinese Optics Letters 2020年第10期18卷 53-57页

作者： Xinkun Wang Kedi Xiong Xin Jin sihua yang MOE Key Laboratory of Laser Life Science&Inst itute of Laser Life Science College of BiophotenicsSouth China Normal UniversityGuangzhou 510631China Guangdong Provincial Key Laboratory of Laser Life Science Collge of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

The previous methods to measure flow speed by photoacoustic microscopy solely focused on either the transverse or the axial flow component, which did not reflect absolute flow speed. Here, we present absolute flow speed maps by combining Doppler bandwidth broadening with volumetric photoacoustic microscopy. Photoacoustic Doppler bandwidth broadening and photoacoustic tomographic images were applied to measure the transverse flow component and the Doppler angle, respectively. Phantom experiments quantitatively demonstrated that ranges of 55° to 90° Doppler angle and 0.5 to 10 mm/s flow speed can be measured. This tomography-assisted method provides the foundation for further measurement in vivo.

关键词： medical optics and biotechnology photoacoustic imaging scanning microscopy flow speed

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