A chorismate mutase from Radopholus similis plays an essential role in pathogenicity

作     者：Sihua Yang Junyi Li Shuai Yang Shiqiao Tang Huizhong Wang Chunling Xu Hui Xie Sihua Yang;Junyi Li;Shuai Yang;Shiqiao Tang;Huizhong Wang;Chunling Xu;Hui Xie

作者机构：Laboratory of Plant Nematology andResearch Centerof Nematodes of Plant QuarantineDepartment of Plant Patholo of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第3期

页      面：923-937页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 

基　　金：funded by the Guangdong Basic and Applied Basic Research Foundation,China(2021A1515011273) he National Natural Science Foundation of China(31071665) 

主　　题：Radopholus similis chorismate mutase plant defense transgenic tomato 

摘      要：In the process of infecting plants, plant parasitic nematodes release a series of proteins that play an essential role in the successful infection and pathogenesis of plant cells and tissues through stylets or body walls. In this study,based on transcriptome data, a chorismate mutase gene of Radopholus similis(RsCM) was identified and cloned,which is a single copy gene specifically expressed in the oesophageal gland and highly expressed in juveniles and females. Transient expression of RsCM in tobacco leaves showed that it was localised in the cytoplasm and nucleus of tobacco leaf cells, which inhibited the pattern-triggered immunity(PTI) induced by flg22, including callose deposition and defence gene expression, and cell death induced by immune elicitors BAX, but could not inhibit cell death induced by immune elicitors Gpa2/RBP-1. The RNA interference(RNAi) transgenic tomato of RsCM obviously inhibited the infection, pathogenicity, and reproduction of R. similis. However, the resistance of the overexpression transgenic tomato of RsCM to R. similis infection was significantly reduced, and the expression levels of two salicylic acid(SA) pathway genes(PR1 and PR5) in roots infected by the nematode were significantly down-regulated,which indicated that RsCM might be involved in the inhibition of SA pathway. The results of this study demonstrate that RsCM suppresses the host immune system and might be a new target for the control of R. similis, which also provides new data for the function and mechanism of CM genes of migratory parasitic plant nematodes.