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Journal of Environmental Sciences 2012年第5期24卷 963-968页

作者： Himanshu Priyadarshi Absar Alam Gireesh-Babu P Rekha Das Pankaj Kishore shivendra kumar Aparna Chaudhari College of Fisheries (CAU) Lembucherra Tripura 799210 India Department of Fish Genetics and Biotechnology Central Institute of Fisheries Education Department of Fish Processing Technology Central Institute of Fisheries Education Department of Fish Nutrition and Biochemistry Central Institute of Fisheries Education Krishi Vigyan Kendra Rajendra Agricultural University

A mercury biosensor was constructed by integrating biosensor genetic elements into E. coli JM109 chromosome in a single copy number, using the attP/attB recombination mechanism of λ phage. The genetic elements used include a regulatory protein gene （merR） along with operator/promoter （O/P） derived from the mercury resistance operon from pDU1358 plasmid of Serratia marcescens. The expression of reporter gene gfp is also controlled by merR/O/P. Integration of the construct into the chromosome was done to increase the stability and precision of the biosensor. This biosensor could detect Hg（Ⅱ） ions in the concentration range of 100–1700 nmol/L, and manifest the result as the expression of GFP. The GFP expression was significantly different （P 0.05） for each concentration of inducing Hg（Ⅱ） ions in the detection range, which reduces the chances of misinterpretation of results. A model using regression method was also derived for the quantification of the concentration of Hg（Ⅱ） in water samples.

关键词： attP/attB integration E. coli biosensor GFP mercury

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Designing a Mini-Core Collection Effectively Representing 3004 Diverse Rice Accessions

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Plant Communications 2020年第5期1卷 141-154页

作者： Angad kumar shivendra kumar Kajol B.M.Singh Manoj Prasad Jitendra K.Thakur Plant Mediator Lab National Institute of Plant Genome ResearchAruna Asaf Ali MargNew Delhi 110067India

Genetic diversity provides the foundation for plant breeding and genetic *** 3000 rice genomes were recently sequenced as part of the 3K Rice Genome(3KRG)*** added four additional Indian rice accessions to create a panel of 3004 ***,such a large collection of germplasm is difficult to preserve and *** construction of core and mini-core collections is an efficient method for the management of genetic *** this study,we developed a mini-core comprising 520 accessions that captured most of the SNPs and represented all of the phenotypes and geographic regions from the original *** mini-core was validated using different statistical analyses and contained representatives from all major rice groups,including japonica,indica,aus/boro,and aromatic/*** association analyses of the mini-core panel efficiently reproduced the marker–trait associations identified in the original *** analysis validated the utility of the mini-core *** the current era with many ongoing large-scale sequencing projects,such a strategy for mini-core design should be useful in many *** rice mini-core collection developed in this study would be valuable for agronomic trait evaluation and useful for rice improvement via marker-assisted molecular breeding.

关键词： rice mini-core SNPs GWAS 3KRG agronomic trait

Palliative surgical bypass for unresectable periampullary carcinoma

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Hepatobiliary & Pancreatic Diseases International 2008年第3期7卷 308-312页

作者： shivendra Singh Ajay kumar Sachdev Adarsh Chaudhary Anil kumar Agarwal Department of Gastrointestinal Surgery Govind Ballabh Pant Hospital&Maulana Azad Medical CollegeNew Delhi 110002India

BACKGROUND:Around 60%to 80%of patients with periampullary carcinoma are unresectable either due to distant metastasis or local vascular *** the advancement of endoscopic interventional procedures,the role of surgical bypass has ***,surgical bypass is still appropriate in patients with unresectable disease discovered at the time of *** study was conducted to assess the results of palliative surgical bypass for patients with unresectable periampullary carcinoma at our hospital,a tertiary referral center of Northern India. METHOD:The study group comprised 204 patients who had undergone surgical bypass for advanced periampullary carcinoma over the last 15 years. RESULTS:Between January 1990 and December 2004,204 patients(128 males,76 females)consisting of 179 patients with carcinoma of head of the pancreas,14 patients with ampullary carcinoma,8 patients with lower end cholangiocarcinoma and 3 patients with duodenal carcinoma underwent surgical *** average age was 51 years (range 20-78 years).Both biliary and gastric bypasses were done in 158(77.45%),biliary bypass alone in 37(18.13%) and gastric bypass alone in 9(4.32%).Biliary bypass was done by Roux-en-Y hepaticojejunostomy,and gastric bypass by retrocolic *** overall postoperative mortality and morbidity were 0.98%and 26.9%,respectively The patients who died had undergone previously endoscopic *** included wound infection in 12.25%of the patients,bile leak in 5.12%,delayed gastric emptying in 5.38%,ascitic leak from drains in 8.8%,and upper gastrointestinal bleeding in 1.96%.The incidences of wound infection and bile leak both were significantly higher in patients who had had preoperative biliary stenting None of the patients who had undergone Roux-en-Y hepati cojejunostomy+retrocolic gastrojejunostomy required any intervention later in their ***:Surgical bypass is a safe procedure with negligible mortality and minimal mo

关键词： surgical bypass periampullary carcinoma

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Efficient One-Pot Access to 2,9-Dihydrothiopyrano[2,3-b]indole Scaffolds Showing Large Stokes Shifts

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Chinese Journal of Chemistry 2015年第11期33卷 1244-1250页

作者： shivendra Singh Sampak Samanta Department of Chemistry Indian Institute of Technology Indore Indore 452017 India

A simple, mild and efficient one-pot approach for the construction of 2-aryl-3-nitro-2,9-dihydrothiopyrano- [2,3-b]indole derivatives has been realized in CH2C12 medium at ambient temperature via three-component tandem reaction of N-protected-2-chloro-3-formylindoles, sodium hydrosulfide and β-substituted nitroolefins/δ-substituted nitrodienes using DABCO （10 mol%） as an organocatalyst, followed by dehydration in the presence of activated molecular sieves （4 A）. The significant advantages of this protocol are simple operation, shorter reaction time, high atom economy, good to high yields （73%-89%） and wider substrate scope. In addition, all the synthesized com- pounds have shown the large positive Stokes shift values （5632-6081 cm ^-1）.

关键词： one-pot MCR 2,9-dihydrothiopyrano[2,3-b]indole organocatalysis molecular sieves large Stokes shifts

Epigenetic effects of ethanol on liver and gastrointestinal injury

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World Journal of Gastroenterology 2006年第33期12卷 5265-5271页

作者： shivendra D Shukla Annayya R Aroor Department of Medical Pharmacology & Physiology University of Missouri School of Medicine Columbia Missouri 65212 United States

Alcohol consumption causes cellular injury. Recent developments indicate that ethanol induces epigenetic alterations, particularly acetylation, methylation of histones, and hypo- and hypermethylation of DNA. This has opened up a new area of interest in ethanol research and is providing novel insight into actions of ethanol at the nucleosomal level in relation to gene expression and patho-physiological consequences. The epigenetic effects are mainly attributable to ethanol metabolic stress (Emess), generated by the oxidative and non-oxidative metabolism of ethanol, and dysregulation of methionine metabolism. Epigenetic changes are important in ethanol-induced hepatic steatosis, fibrosis, carcinoma and gastrointestinal injury. This editorial highlights these new advances and its future potential.

关键词： Alcohol Alcoholic liver disease DNA methylation Epigenetics Ethanol Gastrointestinal injury Histone modifications Liver injury

Molecular targets of cancer chemoprevention by garlic-derived organosulfides

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Acta Pharmacologica Sinica 2007年第9期28卷 1355-1364页

作者： Anna HERMAN-ANTOSIEWICZ Anna A POWOLNY shivendra V SINGH Department of Molecular Biology University of Gda(sk Kladki 2480-822 GdaskPoland Department of Pharmacology and University of Pittsburgh Cancer Institute University of Pittsburgh School of MedicinePittsburghPennsylvania 15213USA

The medicinal benefits of Allium vegetables,especially garlic,have been notedthroughout recorded *** known health benefits of Allium vegetablesand their constituents include cardiovascular protective effects,stimulation ofimmune function,reduction of blood glucose level,radioprotection,improvementof memory loss,protection against microbial,viral and fungal infections,as well asanticancer ***-based case control studies have suggested aninverse correlation between dietary intake of Allium vegetables and the risk ofdifferent types of *** anticarcinogenic effect of Allium vegetables in-cluding garlic is attributed to organosulfur compounds (OSC),which are highlyeffective in affording protection against cancer in animal models induced by avariety of chemical *** recent studies have shown that certainnaturally occurring OSC analogues can suppress proliferation of cancer cells inculture and in *** OSC-induced changes in the proliferation of cancer cellsare frequently associated with perturbations in cell cycle progression and induc-tion of G2/M phase *** OSC have also been demonstrated to induceapoptosis via the intrinsic pathway by altering the ratio of the Bcl-2 family ofproteins both in cell culture and in in vivo ***-angiogenic activity forgarlic-derived OSC has also been *** article summarizes currentknowledge on molecular targets of cancer chemoprevention by OSC.

关键词： garlic organosulfides apoptosis cell cycle angiogenesis chemoprevention

Binge ethanol intake in chronically exposed rat liver decreases LDL-receptor and increases angiotensinogen gene expression

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World Journal of Hepatology 2011年第9期3卷 250-255页

作者： Annayya R Aroor shivendra D Shukla Department of Medical Pharmacology and Physiology University of Missouri School of Medicine

AIM: To investigated the status of low-density lipoprotein (LDL)-receptor and angiotensionogen gene expression in rats treated chronically with ethanol followed by binge administration, a model that mimics the human scenario. METHODS: Rats were chronically treated with ethanol in liquid diet for 4 wk followed by a single binge mode of ethanol administration (5 mg/kg body weight). Samples were processed 4 h after binge ethanol administration (chronic ethanol binge). Control rats were fed isocaloric diet. In the control for binge, ethanol was replaced by water. Expression of mRNA for angioten-sinogen, c-fos and LDL-receptor, and nuclear accumulation of phospho-extracellular regulated kinases (ERK)1/2 and ERK1/2 protein were examined. RESULTS: Binge ethanol administration in chronically treated rats caused increase in steatosis and necrosis. Chronic ethanol alone had negligible effect on mRNA levels of LDL-receptor, or on the levels of nuclear ERK1/2 and phospho-ERK1/2. But, chronic ethanol followed by binge caused a decrease in LDL-receptor mRNA, and also decreased the levels of ERK1/2 and phospho-ERK1/2 in the nuclear compartment. On the other hand, chronic ethanol-binge increased mRNA expression of angiotensinogen and c-fos. CONCLUSION: Binge ethanol after chronic exposure, causes transcriptional dysregulation of LDL-receptor and angiotensinogen genes, both cardiovascular risk factors.

关键词： Alcoholic liver injury Angiotensinogen Ethanol binge Extracellular regulated kinases1/2 Low-density lipoproteun-receptor Plasminogen activator inhibitor-1

Multimodality management of gallbladder cancer can lead to a better outcome:Experience from a tertiary care oncology centre in North India

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World Journal of Gastroenterology 2021年第45期27卷 7813-7830页

作者： Shaifali Goel Abhishek Aggarwal Assif Iqbal Vineet Talwar Swarupa Mitra shivendra Singh Department of GI and HPB Oncosurgery Rajiv Gandhi Cancer Institute and Research CenterDelhi 110085DelhiIndia Department of Medical Oncology Rajiv Gandhi Cancer Institute and Research CenterDelhi 110085DelhiIndia Department of Radiation Oncology Rajiv Gandhi Cancer Institute and Research CenterDelhi 110085DelhiIndia

BACKGROUND Surgical resection is a treatment of choice for gallbladder cancer(GBC)patients but only 10%of patients have a resectable disease at *** after surgical resection,overall survival(OS)has been poor due to high rates of *** of surgery and systemic therapy can improve outcomes in this aggressive *** To summarize our single-center experience with multimodality management of resectable GBC *** Data of all patients undergoing surgery for suspected GBC from January 2012 to December 2018 was retrieved from a prospectively maintained electronic *** extracted included demographics,operative and perioperative details,histopathology,neoadjuvant/adjuvant therapy,follow-up,and *** know the factors associated with recurrence and OS,univariate and multivariate analysis was done using log rank test and cox proportional hazard analysis for categorical and continuous variables,*** analysis was done using multiple regression *** Of 274 patients with GBC taken up for surgical resection,172(62.7%)were female and the median age was 56 *** exploration,102 patients were found to have a metastatic or unresectable disease(distant metastasis in 66 and locally unresectable in 34).Of 172 patients who finally underwent surgery,93(54%)underwent wedge resection followed by anatomical segment IVb/V resection in 66(38.4%)and modified extended right hepatectomy in 12(7%)*** postoperative mortality at 90 d was 4.6%.During a median follow-up period of 20 mo,71(41.2%)patients developed *** 1-,3-,and 5-years OS rates were 86.5%,56%,and 43.5%,*** 1-and 3-year disease free survival(DFS)rates were 75%and 49.2%,*** multivariate analysis,inferior OS was seen with pT3/T4 tumor(P=0.0001),perineural invasion(P=0.0096),and R+resection(P=0.0125).However,only pT3/T4 tumors were associated with a poor DFS(P<0.0001).CONCLUSION Multim

关键词： Gallbladder cancer Multimodality Surgical resection Adjuvant Chemotherapy Chemoradiotherapy

Anti-yeast activities of Origanum oil against human pathogenic yeasts

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Advances in Bioscience and Biotechnology 2011年第2期2卷 103-107页

作者： Amber Adams Satyanshu kumar John Clauson shivendra Sahi 不详

Outbreak of autoimmune diseases by pathogenic yeasts has led to a serious medical threat. As these organisms evolve resistance to existing antifungal drugs, the concern could be further compounded. The realm of plant derived products offers a wide spectrum of potentially valuable alternatives to the existing synthetic fungicides. Essential oils from sev-eral medicinal plants have been shown to exhibit pharmacological attributes. In the present study, anti-yeast properties of Oregano essential oil (OEO) were examined in vitro against four human patho-genic yeasts i.e., Candida albicans, Cryptococcus al-bidus, Cryptococcus neoformans and Rhodotorula ru-brum. OEO concentration of 200 μg/mL was found to be growth inhibitory against all four yeasts examined, thereby showing its potential to function as a natural anti-yeast agent.

关键词： Origanum Vulgare Candida Albicans Cryptococcus Neoformans Rhodotorula Rubrum Cryptococcus Albidus

Pancreatic ductal adenocarcinoma associated with pancreatic ductal intraepithelial neoplasia:report of a case

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Hepatobiliary & Pancreatic Diseases International 2007年第5期6卷 553-556页

作者： Rajkumar Krishnasamy Shaleen Agarwal shivendra Singh Sunil Puri Puja Sakhuja Anil K Agarwal Department of Gastrointestinal Surgery Govind Ballabh Pant Hospital and Maulana Azad Medical College(Delhi University)New Delhi 110002India Department of Radiology Govind Ballabh Pant Hospital and Maulana Azad Medical College(Delhi University)New Delhi 110002 Department of Pathology Govind Ballabh Pant Hospital and Maulana Azad Medical College(Delhi University)New Delhi 110002

BACKGROUND:The presence of pancreatic ductal intraepithelial neoplasia in patients with chronic pancreatitis is a risk factor for development of pancreatic adenocarcinoma. METHOD:A case of pancreatic ductal adenocarcinoma associated with pancreatic ductal intraepithelial neoplasia was diagnosed in the setting of chronic pancreatitis. RESULTS:Distal pancreatectomy combined with splenec- tomy was performed with a diagnosis of pancreatic body *** examination suggested adenocarcinoma associated with pancreatic ductal intraepithelial *** tumor was detected in the remaining head of the pancreas,for which a total pancreatectomy was done. CONCLUSIONS:When a patient with pancreatic ductal intraepithelial neoplasia associated with adenocarcinoma of the pancreas in the setting of chronic pancreatitis is at an increased risk of recurrence in the remaining pancreatic parenchyma,total pancreatectomy may be feasible.

关键词： pancreatectomy pancreatic ductal carcinoma chronic pancreatitis

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