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Identification of Epinephelus malabaricus and Epinephelus coioides using DNA markers

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Acta Oceanologica Sinica 2007年第1期26卷 122-129页

作者： wang shifeng DU Jiaying wang Jun DING Shaoxiong College of Oceanography and Environment Science Xiamen University Xiamen 361005 China

Using multi-molecular marker technologies and based on morphological criteria, the genetic relationship between Epinepheelus malabaricus and E, coioides was examined in the hope of resolving the long-standing issue of identifying these two species. Results showed that：（ 1 ） E. coioides and E. malabaricus should be identified as two species, the consistency of mitochondrial DNA cytochrome b gene sequence between E. malabaricus and E. coioides is 94.4%, and the genetic similarity by AFLP was 0.753 9 ; （2） Hybridization exists between E. malabaricus and E. coioides, the specific RAPD and AFLP fragments are found to be useful in the identification of these two species, and the genetic properties （both with exterior and inheritance） of hybrid is significantly biased to the male parents ; and （3） AFLP was a potentially powerful tool in constructing the genetic linkage map for these two groupers.

关键词： Epinephelus malabaricus Epinephelus coioides hybridize molecular identification

Interictal Electrophysiological Source Imaging Based on Realistic Epilepsy Head Model in Presurgical Evaluation:A Prospective Study

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Chinese Journal of Electrical Engineering 2023年第1期9卷 61-70页

作者： Ruowei Qu Zhaonan wang shifeng wang Le wang Alan wang Guizhi Xu State Key Laboratory of Reliability and Intelligence of Electrical Equipment Hebei University of TechnologyTianjin 300401China Tianjin Universal Medical Imaging Diagnostic Center Tianjin 300110China Department of Functional Neurosurgery Huanhu HospitalTianjin 300350China Auckland Bioengineering Institute The University of AucklandGraftonAuckland 1010New Zealand

Invasive techniques are becoming increasingly important in the presurgical evaluation of *** the electrophysiological source imaging(ESI)of interictal scalp electroencephalography(EEG)to localize the epileptogenic zone remains a *** accuracy of the preoperative localization of the epileptogenic zone is key to curing *** T1 MRI and the boundary element method were used to build the realistic head *** solve the inverse problem,the distributed inverse solution and equivalent current dipole(ECD)methods were employed to locate the epileptogenic ***,a combination of inverse solution algorithms and Granger causality connectivity measures was *** ECD method exhibited excellent focalization in lateralization and localization,achieving a coincidence rate of 99.02%(p<0.05)with the stereo *** combination of ECD and the directed transfer function led to excellent matching between the information flow obtained from intracranial and scalp EEG *** ECD inverse solution method showed the highest performance and could extract the discharge information at the cortex level from noninvasive low-density EEG ***,the accurate preoperative localization of the epileptogenic zone could reduce the number of intracranial electrode implantations required.

关键词： Epilepsy epileptogenic zone realistic head model functional brain connectivity ESI

Sedimentary Record of the Planation Surface in the Hoh Xil Region of the Northern Tibet Plateau

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Acta Geologica Sinica(English Edition) 2000年第4期74卷 827-835页

作者： YI Haisheng wang Chengshan LIU Shun LIU Zhifei wang shifeng

Miocene marl is the most widespread Tertiary stratigraphic record in the northern Tibet Plateau, termed the Wudaoliang Group in the Hoh Xil region and the correlative Suonahu Formation in the Qiangtang region. The uniform marl overlies red beds of the Eocene-Oligocene Fenghuoshan Group. The Wudaoliang Group is generally 100-400 m thick, but the thickest strata are 700-1300 m, located in the Haidinghu (Maiding Lake) and Tuotuohe (Tuotuo River) regions respectively. Based on observations from eight measured sections and outcrops, the thin-bedded marl, which varies in colour from grey-white to light brown-grey, is explained as a large-scale or serial lacustrine deposit stretching throughout northern *** Wudaoliang Group commonly crops out on geographic lowland at an average elevation of 4600 m above sea level within the mountain chains, showing concordant summit levels, e.g. the Fenghuoshan and Bairizhajia Mountains. These mountains with a flat ridge are considered to be remains of the palaeo-planation surface. However, the spatial distribution of the Wudaoliang Group is not confined by the current mountain-basin landform configuration. We have observed the Miocene Wudaoliang Group marl exposed on a 5233 m-high mountain peak. The largest difference in height between the current lake level and the mesa crest is 600 m; the maximum dip angle is 25°, but usually below 10°, which is obviously different from the Fenghuoshan Group red beds with moderate to strong structural deformation. The horizon of the Wudaoliang Group thin-bedded marl and its widespread occurrence throughout the northern Tibetan Plateau can only be reasonably inferred to a sedimentary record relevant to the palaeo-planation. Its deposition occurred on primary land floor by erosion at 20 Ma and its bed surface is a typical sign of geomorphic rise and collapse after the erosion.

关键词： Miocene sedimentary record planation surface uplift collapse Hoh Xil northern Tibet

Utilization of Bayer Red Mud Derived from Bauxite for Belite-Ferroaluminate Cement Production

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Journal of Renewable Materials 2020年第11期8卷 1531-1541页

作者： Yanrong Zhao Ping Chen shifeng wang Yaxiong Ji Yuanhao wang Bolin Wu Rongjin Liu College of Material Science and Engineering Guilin University of TechnologyGuilin541004China Key Laboratory of New Processing Technology for Nonferrous Metal&Materials Ministry of EducationGuilin University of TechnologyGuilin541004China Guangxi Key Laboratory of New Energy and Building Energy Saving Guilin541004China Guangxi Engineering and Technology Center for Utilization of Industrial Waste Residue in Building Materials Guilin541004China Department of Physics College of ScienceTibet UniversityLhasa850000China

Bayer red mud(BRM)is a kind of industrial solid waste characterized by huge volume and high *** disposal generates serious environmental pollution and occupies a large number of *** utilization and recycling of BRM is currently a crucial issue and needs to be addressed as soon as *** chemical composition of BRM is similar to cement *** this study,the feasibility of preparing Belite-ferroaluminate clinker(BFAC)with different BRM was *** physical properties,mechanics performance,radioactivity levels and trace harmful metals leaching were ***,BEI and EDS were used to characterize the mineral formation,and SEM is used to reveal the solidified mechanism of trace harmful *** results show that the preparation of BFAC using a certain amount of BRM was *** formed phases in clinkers mainly included C_(4)A_(3)Š,C_(2)S and C_(4)*** flexural strength and compressive strength of BFAC at 3 days increased whereas 28 and 90 days decreased with the increase of BRM due to the formation of higher C_(4)AF and lower C_(2)*** formation of large amounts of Al_(2)O_(3)·3H_(2)O gel and Fe_(2)O_(3)·3H_(2)O gel in hydration products enhanced the adsorption capability to heavy metals and other *** trace harmful metal concentration in the leaching solution was much less than the upper *** radioactivity level of leaching solution was close to natural radioactive *** is safe as raw material of BFAC.

关键词： Bayer red mud belite-ferroaluminate clinker leaching of harmful metal radioactivity levels phosphogypsum

Antigenicity analysis of Vibrio harveyi TS-628 strain

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Frontiers in Biology 2007年第3期2卷 263-267页

作者： QIN Yingxue wang Jun wang shifeng YAN Qingpi Department of Oceanography Institute of Subtropic OceanXiamen UniversityXiamen 361000China Fisheries College Jimei UniversityXiamen 361021China

Vibrio harveyi,the major causative agent of vib-riosis,affects a diverse range of marine cultured organisms over a wide geographical ***,reports about screening the effective antigen and research on vaccines of *** are ***,lipopolysaccharide(LPS)and outer membrane proteins(OMP)are major immunogenic antigens in many Gram-negative *** this study,the flagellin,OMP and LPS of the *** TS-628 strain iso-lated from infected groupers were extracted and Western blot analysis was used to detect the antigenicity of these *** of the Western blot assay reveal that there are four positive flagellin bands:35 kDa,38 kDa,43 kDa,and 52 kDa,of which the 43 kDa and 52 kDa bands displayed the strongest positive *** are five positive OMPbands about 35 kDa,38 kDa,43 kDa,47 kDa,and 52 kDa,of which the 43 kDa appeared to have the strongest positive reaction although the other four proteins also displayed strong ***,LPS is Western *** results indicate that the 43 kDa and 52 kDa flagellin and OMP of size 43 kDa,52 kDa can be candidates for developing vaccines against ***.

关键词： Vibrio harveyi flagellin LPS OMP antigenicity

Magnetostratigraphy of the Zanda basin in southwest Tibet Plateau and its tectonic implications

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Chinese Science Bulletin 2008年第9期53卷 1393-1400页

作者： wang shifeng ZHANG WeiLin FANG XiaoMin DAI Shuang Oliver KEMPF Institute of Tibetan Plateau Research Chinese Academy Sciences Beijing 100085 China Key Laboratory of Western China's Environmental Systems Ministry of Education of China & College of Resources and Environ- ment Lanzhou University Lanzhou730000 China Institut fer Umweltgeologie Technische University Braunschweig Braunschweig 38106 Germany

The Zanda basin is one of the very important basins at the north slope of the Himalaya Range. Thus the study of the basin strata will provide critical information about the tectonic evolution of the Himalayan Orogenic Belt. 268 oriented block samples were collected in the 750-m-thick sections of the Zanda ba- sin. The characteristic remanent magnetization (ChRM) was isolated that decays linearly to the origin between 500oC and 690oC for most studied samples. An age range of 9.5―2.6 Ma was estimated from the correlation between our observed polarity column and the Geomagnetic Polarity Time Scale (GPTS). The age of the Zanda basin does not support the models that the South Tibetan Detach system (STDS) is one of the basin controlling faults. Given the sedimentological features in the basin and the tectonic features at the north edge of the basin, the Zanda basin was a half graben that was possibly controlled by the Karakorum fault on the northeast.

关键词：盆地磁性地层学断层西藏南部

Assessment of internal controls for data normalization of gene expression after different bacterial stimulation by quantitative real-time PCR in golden pompano Trachinotus blochii

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Journal of Oceanology and Limnology 2020年第2期38卷 480-489页

作者： CHEN Xiaojuan ZHANG Xiaoqi SUN Yun TU Zhigang CAO Zhenjie wang shifeng ZHOU Yongcan State Key Laboratory of Marine Resource Utilization in South China Sea Hainan UniversityHaikou 570228China Hainan Academy of Ocean and Fisheries Sciences Haikou 570228China

Trachinotus blochii is one of the important commercial fish *** this study,we aim to confirm the reliability reference genes in *** during different bacterial challenge through quantitative real-time PCR(qRT-PCR).The expression of the seven selected genes in four immune organs(i.e.,spleen,kidney,intestine,and gill)stimulated with Vibrio harveyi,Edwardsiella tarda,and Streptococcus agalactiae were determined by *** PCR data was analyzed using the geNorm and NormFinder *** results showed the selection of the internal controls should be tissue specific when studying gene expression in response to bacterial *** 48 h of stimulation with ***,geNorm ranked EF1 A/Actin,18 S rRNA/B2M,UBCE/B2M,and 18 S rRNA/B2M,as the most stably expressed genes in spleen,kidney,intestine,and gill,*** 48 h of stimulation with ***,geNorm ranked 18 S rRNA/EF1 A,18 S rRNA/B2M,B2M/RPL13,and 18 S rRNA/EF1 A,as the most stably expressed genes in spleen,kidney,intestine,and gill,*** 48 h of stimulation with ***,18 S rRNA/EF1 A,18 S rRNA/B2 M,B2 M/Actin,and 18 S rRNA/B2M were ranked as the most stably expressed genes in spleen,kidney,intestine,and gill,*** to the results analyzed by geNorm,reference genes received similar rankings when using NormFinder *** results showed that the reference genes appeared to be not only tissue specific,but also specific to the infecting species of *** one gene is preferred when *** were infected by bacteria,18 S rRNA,B2M,B2M,18 S rRNA may be used in spleen,kidney,intestine,and gill,respectively.

关键词： Trachinotus blochii housekeeping gene expression stability reference gene

A multiscale study of the penetrationenhancing mechanism of menthol

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Journal of Traditional Chinese Medical Sciences 2019年第4期6卷 347-354页

作者： Liping Chen Lina Ma Shufang Yang Xiaowen Wu Xingxing Dai shifeng wang Xinyuan Shi School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 102488China Key Laboratory for Production Process Control and Quality Evaluation of Traditional Chinese Medicine Beijing Municipal Science&Technology CommissionBeijing 100029China Department of Molecular Cellular and Developmental BiologyUniversity of ColoradoBoulderCO 80309USA

Objective:Transdermal drug delivery systems represent a critical focus in the pharmaceutics field;however,their use is limited by the fact that many drugs usually pass through the skin with low *** is a common penetration enhancer because of its high penetration-enhancing efficiency and *** research aimed to reveal the penetrationenhancing mechanisms of menthol via a multiscale ***:First,the interaction of menthol with the stratum corneum was studied using vertical Franz diffusion cells obtained from the abdominal skin of rats as a ***,the skin samples were observed via transmission electron ***,the interaction of different concentrations of menthol with a mixed lipid model of the stratum corneum was investigated via molecular dynamics simulation using the GROMOS 54A7 force field on a microcosmic ***:At concentrations of 3.5%or lower,menthol changed the original structure of the stratum corneum to varying degrees,which increased its fluidity and facilitated the permeation and storage of *** increased the fluidity of the stratum corneum mainly via two ***,menthol had strong hydrogen-bonding capability,and it could compete for the lipidelipid hydrogen bonding sites,thereby weakening the stability of the hydrogenbonding network connecting the skin *** addition,menthol had strong affinity for cholesterol,probably due to their similar molecular structures,suggesting that the incorporation of menthol would increase the fluidity of the lipid membrane similarly to ***:The penetration-enhancing mechanism of menthol was explained using in vitro and molecular dynamics simulation *** findings may advance the basic research of transdermal drug delivery systems and facilitate the discoveries of novel penetration enhancers.

关键词： Menthol Molecular dynamics simulations Permeability

Classification research of TCM pulse conditions based on multi-label voice analysis

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Journal of Traditional Chinese Medical Sciences 2024年第2期11卷 172-179页

作者： Haoran Shen Junjie Cao Lin Zhang Jing Li Jianghong Liu Zhiyuan Chu shifeng wang Yanjiang Qiao Key Laboratory of TCM-information Engineer of State Administration of TCM School of Chinese Materia MedicaBeijing University of Chinese MedicineBeijing102488China School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China Sinosense Technology Co. LtdBeijing100141China

Objective:To explore the feasibility of remotely obtaining complex information on traditional Chinese medicine(TCM)pulse conditions through voice ***: We used multi-label pulse conditions as the entry point and modeled and analyzed TCM pulse diagnosis by combining voice analysis and machine *** features were extracted from voice recordings in the TCM pulse condition *** obtained features were combined with information from tongue and facial diagnoses.A multi-label pulse condition voice classification DNN model was built using 10-fold cross-validation,and the modeling methods were validated using publicly available ***: The analysis showed that the proposed method achieved an accuracy of 92.59%on the public *** accuracies of the three single-label pulse manifestation models in the test set were 94.27%,96.35%,and 95.39%.The absolute accuracy of the multi-label model was 92.74%.Conclusion: Voice data analysis may serve as a remote adjunct to the TCM diagnostic method for pulse condition assessment.

关键词： Pulse conditions TCM pulse diagnosis Voice analysis Multi-label classification Machine learning