Identification of Epinephelus malabaricus and Epinephelus coioides using DNA markers

作     者：WANG Shifeng DU Jiaying WANG Jun DING Shaoxiong 

作者机构：College of Oceanography and Environment Science Xiamen University Xiamen 361005 China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2007年第26卷第1期

页      面：122-129页

核心收录：

学科分类：0710[理学-生物学] 090801[农学-水产养殖] 0908[农学-水产] 0707[理学-海洋科学] 09[农学] 

基　　金：This study was supported by the National Natural Science Foundation of China under contract Nos 40306023 and 40576064 the Important Program of Science and Technology of Fujian Province in China under contract No.2004NZ03 

主　　题：Epinephelus malabaricus Epinephelus coioides hybridize molecular identification 

摘      要：Using multi-molecular marker technologies and based on morphological criteria, the genetic relationship between Epinepheelus malabaricus and E, coioides was examined in the hope of resolving the long-standing issue of identifying these two species. Results showed that： （ 1 ） E. coioides and E. malabaricus should be identified as two species, the consistency of mitochondrial DNA cytochrome b gene sequence between E. malabaricus and E. coioides is 94.4%, and the genetic similarity by AFLP was 0.753 9 ; （2） Hybridization exists between E. malabaricus and E. coioides, the specific RAPD and AFLP fragments are found to be useful in the identification of these two species, and the genetic properties （both with exterior and inheritance） of hybrid is significantly biased to the male parents ; and （3） AFLP was a potentially powerful tool in constructing the genetic linkage map for these two groupers.