Assessment of internal controls for data normalization of gene expression after different bacterial stimulation by quantitative real-time PCR in golden pompano Trachinotus blochii

作     者：CHEN Xiaojuan ZHANG Xiaoqi SUN Yun TU Zhigang CAO Zhenjie WANG Shifeng ZHOU Yongcan CHEN Xiaojuan;ZHANG Xiaoqi;SUN Yun;TU Zhigang;CAO Zhenjie;WANG Shifeng;ZHOU Yongcan

作者机构：State Key Laboratory of Marine Resource Utilization in South China SeaHainan UniversityHaikou 570228China Hainan Academy of Ocean and Fisheries SciencesHaikou 570228China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2020年第38卷第2期

页      面：480-489页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：Supported by the National Natural Science Foundation of China(No.41666006,31702379) the Nanhai Famous Youth Project 

主　　题：Trachinotus blochii housekeeping gene expression stability reference gene 

摘      要：Trachinotus blochii is one of the important commercial fish *** this study,we aim to confirm the reliability reference genes in *** during different bacterial challenge through quantitative real-time PCR(qRT-PCR).The expression of the seven selected genes in four immune organs(i.e.,spleen,kidney,intestine,and gill)stimulated with Vibrio harveyi,Edwardsiella tarda,and Streptococcus agalactiae were determined by *** PCR data was analyzed using the geNorm and NormFinder *** results showed the selection of the internal controls should be tissue specific when studying gene expression in response to bacterial *** 48 h of stimulation with ***,geNorm ranked EF1 A/Actin,18 S rRNA/B2M,UBCE/B2M,and 18 S rRNA/B2M,as the most stably expressed genes in spleen,kidney,intestine,and gill,*** 48 h of stimulation with ***,geNorm ranked 18 S rRNA/EF1 A,18 S rRNA/B2M,B2M/RPL13,and 18 S rRNA/EF1 A,as the most stably expressed genes in spleen,kidney,intestine,and gill,*** 48 h of stimulation with ***,18 S rRNA/EF1 A,18 S rRNA/B2 M,B2 M/Actin,and 18 S rRNA/B2M were ranked as the most stably expressed genes in spleen,kidney,intestine,and gill,*** to the results analyzed by geNorm,reference genes received similar rankings when using NormFinder *** results showed that the reference genes appeared to be not only tissue specific,but also specific to the infecting species of *** one gene is preferred when *** were infected by bacteria,18 S rRNA,B2M,B2M,18 S rRNA may be used in spleen,kidney,intestine,and gill,respectively.