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Neuroscience Bulletin 2021年第6期37卷 789-803页

作者： Wenhao Zhu Hao Huang Shiqi Yang Xiang Luo Wenzhen Zhu shabei xu Qi Meng Chengchao Zuo Yong Liu Wei Wang Alzheimer’s Disease Neuroimaging Initiative Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Radiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Brainnetome Center and National Laboratory of Pattern Recognition Institute of AutomationChinese Academy of SciencesBeijing 100190China School of Artificial Intelligence Beijing University of Posts and TelecommunicationsBeijing 100876China University of the Chinese Academy of Sciences Beijing 100049China 不详

Grey matter(GM)alterations may contribute to cognitive decline in individuals with white matter hyperintensities(WMH)but no consensus has yet ***,we investigated cortical thickness and grey matter volume in 23 WMH patients with mild cognitive impairment(WMH-MCI),43 WMH patients without cognitive impairment,and 55 healthy *** WMH groups showed GM atrophy in the bilateral thalamus,fronto-insular cortices,and several parietal-temporal regions,and the WMH-MCI group showed more extensive and severe GM *** GM atrophy in the thalamus and fronto-insular cortices was associated with cognitive decline in the WMH-MCI patients and may mediate the relationship between WMH and cognition in WMH ***,the main results were well replicated in an independent dataset from the Alzheimer's Disease Neuroimaging Initiative database and in other control *** comprehensive results provide robust evidence of specific GM alterations underlying WMH and subsequent cognitive impairment.

关键词： White matter hyperintensities Cognitive impairment Cortical thickness Grey matter volume Replication

Reactive changes in astrocytes, and delayed neuronal death, in the rat hippocampal CA1 region following cerebral ischemia/reperfusion

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Neural Regeneration Research 2009年第1期4卷 36-41页

作者： Guiqing Zhang Xiang Luo Zhiyuan Yu Chao Ma shabei xu Wei Wang Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China Department of Rehabilitation and Psychology First Affiliated Hospital Medical College Xinjiang Shihezi University Shihezi 832008 Xinjiang Uygur Autonomous Region China Normal College Xinjiang Shihezi University Shihezi 832000 Xinjiang Uygur Autonomous Region China

BACKGROUND： Blood supply to the hippocampus is not provided by the middle cerebral artery. However, previous studies have shown that delayed neuronal death in the hippocampus may occur following focal cerebral ischemia induced by middle cerebral artery occlusion. OBJECTIVE： To observe the relationship between reactive changes in hippocampal astrocytes and delayed neuronal death in the hippocampal CA1 region following middle cerebral artery occlusion. DESIGN, TIME AND SETTING： The immunohistochemical, randomized, controlled animal study was performed at the Laboratory of Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, from July to November 2007. MATERIALS： Rabbit anti-glial fibrillary acidic protein （GFAP）（Neomarkers, USA）, goat anti-rabbit IgG （Sigma, USA） and ApoAlert apoptosis detection kit （Biosciences Clontech, USA） were used in this study. METHODS： A total of 42 healthy adult male Wistar rats, aged 3–5 months, were randomly divided into a sham operation group （n = 6） and a cerebral ischemia/reperfusion group （n = 36）. In the cerebral ischemia/reperfusion group, cerebral ischemia/reperfusion models were created by middle cerebral artery occlusion. In the sham operation group, the thread was only inserted into the initial region of the internal carotid artery, and middle cerebral artery occlusion was not induced. Rats in the cerebral ischemia/reperfusion group were assigned to a delayed neuronal death （＋） subgroup and a delayed neuronal death （–） subgroup, according to the occurrence of delayed neuronal death in the ischemic side of the hippocampal CA1 region following cerebral ischemia. MAIN OUTCOME MEASURES： Delayed neuronal death in the hippocampal CA1 region was measured by Nissl staining. GFAP expression and delayed neuronal death changes were measured in the rat hippocampal CA1 region at the ischemic hemisphere by double staining for GFAP and TUNEL. RESULTS： After 3 days of ischemia

关键词： cerebral ischemia hippocampus delayed neuronal death astrocytes glial fibrillaryacidic protein

Morphological properties and proliferation analysis of olfactory ensheathing cells seeded onto three-dimensional collagen-heparan sulfate biological scaffolds

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Neural Regeneration Research 2012年第16期7卷 1213-1219页

作者： Na Liu Zhouping Tang Zhiyuan Yu Minjie Xie Yu Zhang Erfang Yang shabei xu Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 HubeiProvince China

This study aimed to examine the differences in the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaffolds and in two-dimensional culture on common flat culture plates. The proliferation rate of olfactory ensheathing cells in three-dimensional culture was higher than that in two-dimensional culture, as detected by an M-I-r assay. In addition, more than half of the olfactory ensheathing cells subcultured using the trypsinization method in three-dimensional culture displayed a spindly Schwann cell-like morphology with extremely long processes, while they showed a flat astrocyte-like morphology in two-dimensional culture. Moreover, spindle-shaped olfactory ensheathing cells tended to adopt an elongated bipolar morphology under both culture conditions. Experimental findings indicate that the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaffolds are better than those in two-dimensional culture.

关键词： morphological properties cell proliferation biological scaffold olfactory ensheathing cells three-dimensional culture neural regeneration

Biocompatibility of a collagen-heparin sulfate scaffold implanted into porcine brain

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Neural Regeneration Research 2010年第3期5卷 191-196页

作者： Zhouping Tang Xingyong Chen Wengao Zeng shabei xu xuewei Xie Ronghua Tang Suiqiang Zhu Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China Department of Neurology Fujian Provincial HospitaI Fuzhou 350001 Fujian Province China

BACKGROUND： Collagen-heparin sulfate scaffolds have been widely used to repair nerve injury and promote nerve regeneration. Previous research has evaluated scaffold biocompatibility by measuring gliocyte proliferation but not neuronal apoptosis. OBJECTIVE： To explore the biocompatibility of collagen-heparin sulfate scaffold in porcine brain by detecting peripheral neural apoptosis and protein expression. DESIGN, TIME AND SETTING： A randomized, controlled animal experiment was performed at the Laboratory of Neurology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, between March and June, 2008. MATERIALS： Rabbit anti-human Bax, Caspase-3 polyclonal antibody, rat anti-human Bcl-2 polyclonal antibody, streptavidin biotin-peroxidase complex （SABC） immunohistochemical kit, and TUNEL kit （Roche, USA） were used in this study. METHODS： Twenty adult piglets were randomly evenly divided into implantation and control groups A collagen-heparin sulfate scaffold was implanted from the anterior fontanelle into the brain in the implantation group. The same puncture but no scaffold implantation was made in the control group. MAIN OUTCOME MEASURES： Cell apoptosis was detected using TUNEL; Bax, Bcl-2, and Caspase-3 expressions were measured using the SABC method. RESULTS： At days 1,3, 7, and 14 after scaffold implantation, a few apoptotic cells were observed in the brain tissues near the puncture site, with more apoptotic cells in the implantation group （P 〈 0.05）. However, both groups showed similar apoptosis levels by day 30 after implantation. Implantation increased Bax, Bcl-2, and Caspase-3 expressions on days 3 and 7 after implantation （P 〈 0.05） but decreased the ratio of Bcl-2 to Bax in the implantation group was significantly lower on days 3 and 7 （P 〈 0.05）, with no significant difference by day 30 after implantation （P 〉 0.05）. CONCLUSION： The collagen-heparin sulfate scaffold has good biocompatibility to porcine brain tis

关键词： biomaterial scaffold collagen heparin sulfate neuronal apoptosis biocompatibility nerve tissue engineering neural regeneration

Glial gap-junction communication is involved in hippocampal damage and cognitive deficits following middle cerebral artery occlusion

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Glial gap-junction communication is involved in hippocampal ...

Glial gap junctional communication involvement in hippocampal damage after middle cerebral artery occlusion

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Minjie XIE,Chenju YI,Xiang LUO,shabei xu,Zhiyuan YU,Bitao BU,Wei WANG~* Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430030,China

＜正＞Stroke induced by middle cerebral artery occlusion（MCAO） is a common cause of adult disability leading to motor-sensory disturbances and cognitive *** is generally accepted that cognitive impairment in stroke patients caused by MCAO results from damage to the cerebral cortex rather than the hippocampus,because the middle cerebral artery does not supply blood to the *** we provide the first evidence that patients with exclusively unilateral MCAO show hippocampal damage characterized by an infarct size-independent atrophy and alterations in neuronal and glial metabolites in the ipsilateral hippocampus as assessed by MRI,in parallel with a significant decline in cognitive *** MCAO also induces delayed shrinkage and pyramidal neuronal death in the ipsilateral hippocampus and an impairment of hippocampal-dependent spatial *** glutamate and pyramidal neuronal intracellular calcium levels were significantly elevated and spreading depression waves were observed in the hippocampus which correlated with neuronal *** gap junctional communication （GJC） with carbenoxolone or down-regulation Cx43 significantly increased the survival of the pyramidal neurons in the ipsilateral hippocampus and improved behavioral ***,Cx43 heterozygous mice showed reduced shrinkage and metabolites abnormality in ipsilateral hippocampus after *** results suggest a significant role of astroglial GJC in MCAO-induced remote hippocampal damage and cognitive impairment.

关键词： astrocyte gap junctional communication hippocampus ischemia

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Glial gap junctional communication involvement in hippocampa...

后疫情时代武汉市“双一流”大学某附属医院师生对“全球医学教育基本要求”的认知调查

中国神经科学学会第十届全国学术会议

作者： Minjie Xie Chenju Yi Xiang Luo shabei xu Zhiyuan Yu Yingxin Tang Wenhao Zhu Yixing Du Lintao Jia Qiang Zhang Qiang Dong Wenzhen Zhu Xia Zhang Bitao Bu Wei Wang Departments of Neurology and Radiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Neurology First Affiliated Hospital Nanjing Medical University Institute of Mental Health Research Departments of Psychiatry and Cellular and Molecular MedicineUniversity of Ottawa Department of Neurology Huashan HospitalMedical College of Fudan University

Objective Most patients with stroke casued by middle cerebral artery occlusion（MCAO） show cognitive deficit that is generally regarded as resulting from damage to the cerebral cortex rather than the *** MCAO induces hippocampal damage and whether this contributes to the cognitive defects remains *** we investigate the hippocampal damage and its correlation to cognitive defects after exclusively unilateral MCAO and the underlying mechanism for that *** Patients were assessed for hippocampal damage by magnetic resonance imaging（MRI） and magnetic resonance angiography（MRA）,and the Mini Mental-Status Evaluation（MMSE） and Rey Auditory Verbal Learning Test（RAVLT） were used to assess for cognitive *** We provide the first evidence that patients with exclusively unilateral MCAO showed hippocampal damage characterized by an infarct-size-independent atrophy and alterations in neuronal and glial metabolites in the ipsilateral hippocampus,in parallel with cognitive *** MCAO also induced delayed shrinkage and pyramidal neuronal deaui in the ipsilateral hippocampus and an impairment of hippocampal-dependent spatial *** Gap junctional communication（GJC） with carbenoxolone or downregulation of connexin43（Cx43） significantly increased the survival of the pyramidal neurons in the ipsilateral hippocampus and improved behavioral ***,Cx43heterozygous mice showed reduced shrinkage and metabolite abnormality in ipsilateral hippocampus after *** Astroglial GJC plays a significant role in MCAO-induced remote hippocampal damage and cognitive *** might be possible to improve the cogmtion in patients with MCAO by manipulating interastrocytic communication via the gap junction channels.

关键词： glial hippocampus gap junction middle cerebral artery occlusion

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中国高等医学教育 2021年第12期 3-5页

作者：施恋徐沙贝华中科技大学同济医学院附属同济医院湖北武汉430000

目的:了解新形势下师生对"全球医学教育基本要求"(GMER)的认知态度并进行异同比较。方法:依据GMER 7个领域内容设置问卷,分别进行抽样调查。结果:共发出问卷470份,收回有效问卷445份,有效问卷率92.55%。不同特征下师生基于GMER 7大领域... 详细信息

目的:了解新形势下师生对"全球医学教育基本要求"(GMER)的认知态度并进行异同比较。方法:依据GMER 7个领域内容设置问卷,分别进行抽样调查。结果:共发出问卷470份,收回有效问卷445份,有效问卷率92.55%。不同特征下师生基于GMER 7大领域认知得分存在显著性差异,师资方面:在GMER领域一(t=-2.354,P=0.021)、领域三(t=-2.419,P=0.019)、领域四(t=-2.215,P=0.033)、领域七(t=-3.206,P=0.003)呈现出显著性差异(P<0.05),学历方面:领域二(F=3.240,P=0.043)、领域三(F=3.209,P=0.045)、领域四(F=5.533,P=0.005)、领域六(F=3.101,P=0.049)、领域七(F=9.491,P=0.000)呈现出显著性差异(P<0.05)。结论:总体看,师生对GMER 7个领域的基本要求普遍认同;比较GMER认知态度,师生均在领域一的认知得分较高,在领域五和领域六认知得分较低,参与了抗击新冠肺炎战斗的临床医生,其职业价值、态度、医学人文和医学伦理领域的认知得分明显高于医学研究生。

关键词：全球医学教育基本要求医学教育认知调查

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偏头痛与缺血性卒中关系的研究进展

神经损伤与功能重建 2024年第2期19卷 94-97页

作者：么瑶徐沙贝华中科技大学同济医学院附属同济医院神经内科武汉430030

偏头痛是一种常见的慢性反复发作性疾病。近年来有越来越多的证据表明,偏头痛尤其是先兆偏头痛与缺血性卒中之间存在关联,但发病机制尚未完全阐明。有研究表明,一些常见的血管危险因素可能会增加偏头痛患者卒中风险。本文主要针对偏头... 详细信息

偏头痛是一种常见的慢性反复发作性疾病。近年来有越来越多的证据表明,偏头痛尤其是先兆偏头痛与缺血性卒中之间存在关联,但发病机制尚未完全阐明。有研究表明,一些常见的血管危险因素可能会增加偏头痛患者卒中风险。本文主要针对偏头痛与缺血性卒中关联的可能机制进行简单综述,旨在提高对偏头痛相关卒中的认识,为临床诊疗提供指导。

关键词：偏头痛缺血性卒中发病机制

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浅论针刺期望值及其临床评价

中国针灸 2017年第2期37卷 215-218页

作者：王华付伊萌邱宇驰徐沙贝陈茜吴松湖北中医药大学针灸骨伤学院/针灸治未病湖北省协同创新中心武汉430061 华中科技大学同济医学院附属同济医院

针刺期望值是指人们对即将采取的针刺手段会产生的效果进行的主观估计,由于在针刺临床随机对照试验中一般会设假针刺组,因此针刺期望值对受试者的影响确实存在,有必要对其进行评价及标准化。不同的针刺期望值评价问题、评价尺度和时间... 详细信息

针刺期望值是指人们对即将采取的针刺手段会产生的效果进行的主观估计,由于在针刺临床随机对照试验中一般会设假针刺组,因此针刺期望值对受试者的影响确实存在,有必要对其进行评价及标准化。不同的针刺期望值评价问题、评价尺度和时间点都是影响针刺期望值评价的因素,都会对评价临床疗效产生影响,因此急需建立统一的评价标准以提高其可信度。

关键词：针刺期望值随机对照试验统一标准

Identification of Common Variants within KCNK17 in Chinese Han Population

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2010年第1期30卷 13-17页

作者：唐洲平丁虎徐宇军徐沙贝 Department of Neurology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology The Institute of Hypertension and Department of Internal Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

KCNK17 is a member of the acid-sensitive subfamily of tandem pore K+ channels,which are open at all membrane potentials and contribute to cellular resting membrane *** genome-wide study(GWA)has shown that variants within KCNK17 confer genetic susceptibility for increasing ischemic *** an effort to discover additional polymorphism(s),we scrutinized the genetic polymorphisms in the *** direct DNA sequencing in 32 individuals,we identified nine sequence variants within the 16kb of whol...

关键词： polymorphism haplotypes KCNK17 ischemic stroke Chinese Han population