Biocompatibility of a collagen-heparin sulfate scaffold implanted into porcine brain

作     者：Zhouping Tang Xingyong Chen Wengao Zeng Shabei Xu Xuewei Xie Ronghua Tang Suiqiang Zhu 

作者机构：Department of Neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China Department of Neurology Fujian Provincial HospitaI Fuzhou 350001 Fujian Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2010年第5卷第3期

页      面：191-196页

核心收录：

学科分类：10[医学] 

基　　金：the National Natural Science Foundation of China  No. 30570628  30770751 

主　　题：biomaterial scaffold collagen heparin sulfate neuronal apoptosis biocompatibility nerve tissue engineering neural regeneration 

摘      要：BACKGROUND： Collagen-heparin sulfate scaffolds have been widely used to repair nerve injury and promote nerve regeneration. Previous research has evaluated scaffold biocompatibility by measuring gliocyte proliferation but not neuronal apoptosis. OBJECTIVE： To explore the biocompatibility of collagen-heparin sulfate scaffold in porcine brain by detecting peripheral neural apoptosis and protein expression. DESIGN, TIME AND SETTING： A randomized, controlled animal experiment was performed at the Laboratory of Neurology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, between March and June, 2008. MATERIALS： Rabbit anti-human Bax, Caspase-3 polyclonal antibody, rat anti-human Bcl-2 polyclonal antibody, streptavidin biotin-peroxidase complex （SABC） immunohistochemical kit, and TUNEL kit （Roche, USA） were used in this study. METHODS： Twenty adult piglets were randomly evenly divided into implantation and control groups A collagen-heparin sulfate scaffold was implanted from the anterior fontanelle into the brain in the implantation group. The same puncture but no scaffold implantation was made in the control group. MAIN OUTCOME MEASURES： Cell apoptosis was detected using TUNEL; Bax, Bcl-2, and Caspase-3 expressions were measured using the SABC method. RESULTS： At days 1,3, 7, and 14 after scaffold implantation, a few apoptotic cells were observed in the brain tissues near the puncture site, with more apoptotic cells in the implantation group （P 〈 0.05）. However, both groups showed similar apoptosis levels by day 30 after implantation. Implantation increased Bax, Bcl-2, and Caspase-3 expressions on days 3 and 7 after implantation （P 〈 0.05） but decreased the ratio of Bcl-2 to Bax in the implantation group was significantly lower on days 3 and 7 （P 〈 0.05）, with no significant difference by day 30 after implantation （P 〉 0.05）. CONCLUSION： The collagen-heparin sulfate scaffold has good biocompatibility to porcine brain tis