检索结果-南通市图书馆

An electropenetrography waveform library for the probing and ingestion behaviors of Culex tarsalis on human hands

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2024年第4期31卷 1165-1186页

作者： Anastasia M.W.Cooper samuel b.Jameson Victoria Pickens Cameron Osborne Elaine A.backus Kristopher Silver Dana N.Mitzel Department of Entomology Kansas State UniversityManhattanKansasUSA Department of Tropical Medicine Tulane University School of Public Health and Tropical MedicineNew OrleansLos AngelesUSA USDA Agricultural Research Service San Joaquin Valley Agricultural Sciences CenterParlierCaliforniaUSA National bio and Agro-Defense Facility USDA Agricultural Research ServiceManhattanKansasUSA

Culex tarsalis Coquillett(Diptera:Culicidae)mosquitoes are capable of vectoring numerous pathogens affecting public and animal ***,the probing behaviors of mosquitoes are poorly understood because they occur in opaque ***(EPG)has the potential to elucidate these behaviors by recording the electrical signals generated during *** used an AC–DC EPG with variable input resistors(Ri levels)to construct a waveform library for *** feeding on human *** events associated with mosquito probing were used to characterize waveforms at four Ri levels and with two electrical current *** optimal settings for EPG recordings of *** probing on human hands was an Ri level of 10^(7)Ohms using an applied signal of 150 millivolts alternating *** for *** included those previously observed and associated with probing behaviors in Aedes aegypti L.(Diptera:Culicidae):waveform families J(surface salivation),K(stylet penetration through the skin),L(types 1 and 2,search for a blood vessel/ingestion site),M(types 1 and 2,ingestion),N(type 1,an unknown behavior which may be a resting and digestion phase),and W(withdrawal).However,we also observed variations in the waveforms not described in ***,which we named types L3,M3,M4,and *** investigation enhances our understanding of mosquito probing *** also provides a new tool for the automated calculation of peak *** work will facilitate future pathogen acquisition and transmission studies and help identify new pest and disease management targets.

关键词： AC-DC blood feeders electrical penetration graph EPG human hands mosquitoes waveform library

Measurement of copy number variation in single cancer cells using rapid-emulsification digital droplet MDA

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2017年第1期3卷 260-266页

作者： samuel C.Kim Gayatri Premasekharan Iain C.Clark Hawi b.Gemeda Pamela L.Paris Adam R.Abate Department of bioengineering and Therapeutic Sciences University of CaliforniaSan Francisco(UCSF)California Institute for Quantitative Biosciences(QB3)San FranciscoSan FranciscoCA 94158USA Department of Urology Division of Hematology&OncologyUniversity of CaliforniaSan Francisco(UCSF)San FranciscoCA 94158USA

Uniform amplification of low-input DNA is important for applications across biology,including single-cell genomics,forensic science,and microbial and viral ***,the requisite biochemical amplification methods are prone to bias,skewing sequence proportions and obscuring signals relating to copy *** droplet multiple displacement amplification enables uniform amplification but requires expert knowledge of microfluidics to generate monodisperse *** addition,existing microfluidic methods are tedious and labor intensive for preparing many ***,we introduce rapid-emulsification multiple displacement amplification,a method to generate monodisperse droplets with a hand-held syringe and hierarchical droplet *** conventional microfluidic devices require >10 min to emulsify a sample,our system requires tens of seconds and yields data of equivalent *** demonstrate the approach by using it to accurately measure copy number variation(CNV)in single cancer cells.

关键词： amplification bias copy number variation ddMDA droplet microfluidics multiple displacement amplification

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于生态学原理的作物品种混合种植建立

Journal of Plant Ecology 2023年第6期16卷 46-60页

作者： Emanuel b.Kopp Pascal A.Niklaus samuel E.Wuest Agroscope Group Breeding ResearchDivision Plant BreedingMüller-Thurgau-Strasse 298820 W鋎enswilSwitzerland Department of Evolutionary biology and Environmental Studies and Zurich-basel Plant Science Center University of ZurichWinterthurerstrasse 1908057 ZurichSwitzerland University of Zurich Research Priority Program on Global Change and biodiversity University of Zurich8057 ZurichSwitzerland

作物品种混合种植具有很多有益效应,包括抑制病原体和增加产量和产量稳定性。然而,这些效应并不一定在所有作物品种混合种植中都出现,多样性带来的效应可能会因作物异质性的增加而抵消。在田间建立作物品种混合种植,如果没有预先预测组... 详细信息

作物品种混合种植具有很多有益效应,包括抑制病原体和增加产量和产量稳定性。然而,这些效应并不一定在所有作物品种混合种植中都出现,多样性带来的效应可能会因作物异质性的增加而抵消。在田间建立作物品种混合种植,如果没有预先预测组合哪些基因型可以产生表现良好的混合种植,会导致工作量过于庞大。因此,需要有效的工具来缩减有希望的品种混合种植组合,并在实验中确定其中哪些组合会带来最大效应。在这里,我们首先综述已有的关于生态多样性实验和当前农业应用效应及其机理,然后讨论应用这些知识开发良好品种混合种植组合时出现的一些主要困难。我们还讨论了解决其中一些问题的非常规方法。我们特别强调基于性状确定好的品种混合种植伙伴的潜力和局限性,并认为非传统性状和性状衍生的指标可能需要基于性状的方法来充分发挥其潜力。具体而言,我们认为可以使用现代遗传和基因组方法确定良好的混合种植伙伴。或者,可以通过组合对环境变化做出不同反应的品种(这些品种可以在标准品种测试试验中轻松识别)来获得良好的混作组合。初步分析结果表明,解释对不同环境响应的生态位差异,可以指示功能互补性并提高混合种植组合的产量及其稳定性。

关键词：农业生态学品种混合种植作物多样性群落生态学植物育种病原物/疾病控制产量稳定性

Heat shock protein 90 inhibition by 17-DMAG lessens disease in the MRL/Ipr mouse model of systemic lupus erythematosus

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2012年第3期9卷 255-266页

作者： samuel K Shimp III Cristen b Chafin Nicole L Regna Sarah E Hammond Molly A Read David L Caudell Marissa Nichole Rylander Christopher M Reilly Virginia Tech-Wake Forest School of biomedical Engineering and Science Virginia Polytechnic Institute and State University Blacksburg VA USA Department of biomedical Sciences and Pathobiology Virginia-Maryland Regional College of Veterinary Medicine Blacksburg VA USA Edward Via College of Osteopathic Medicine Blacksburg VA USA Department of Mechanical Engineering Virginia Polytechinc Institue and State University Blacksburg VA USA

Elevated expression of heat shock protein 90 （HSP90） has been found in kidneys and serum of systemic lupus erythematosus （SLE） patients and MRLIMp-FasIprIFasJpr（MRLIIpr） autoimmune mice. We investigated if inhibition of HSP90 would reduce disease in MRL/ Ipr mice. In vitro, pretreatment of mesangial cells with HSP90 inhibitor Geldanamycin prior to immune-stimulation showed reduced expression of IL-6, IL-12 and NO. In vivo, we found HSP90 expression was elevated in MRL/Ipr kidneys when compared to C57bL/6 mice and MRIJIpr mice treated with HSP90 inhibitor 17-DMAG. MRIJIpr mice treated with 17-DMAG showed decreased proteinuria and reduced serum anti-dsDNA antibody production. Glomerulonephritis and glomerular IgG and C3 were not significantly affected by administration of 17-DMAG in MRIJIpr. 17-DMAG increased CD8＋ T cells, reduced double-negative T cells, decreased the CD4/CD8 ratio and reduced follicular b cells. These studies suggest that HSP90 may play a role in regulating T-cell differentiation and activation and that HSP90 inhibition may reduce inflammation in lupus.

关键词： Geldanamycin heat shock protein 90 MRUIpr systemic lupus erythematosus 17-DMAG

Stabilized composition of 26 mg/mL of high molecular weight HA for subcutaneous injection to improve skin quality

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plastic and Aesthetic Research 2022年第1期9卷 564-575页

作者： samuel Gavard Molliard Jérémie bon bétemps basste Hadjab Anas Ghazal Mhd badi Marco Cerrano Department of Research and Development Kylane Laboratoires SAPlan-les-Ouates 1228Switzerland Department of Clinic Derma ClinicJeddah 23432Saudi Arabia Department of Clinic Art ClinicRiyadh 12432Saudi Arabia Department of Clinic Entourage CliniqueLausanne 1003Switzerland

Aim:Skin firmness is one of the key parameters to define skin quality and facial *** the minimally invasive anti-aging strategies,hyaluronic acid(HA)injection is widely accepted to improve skin *** most of the available HA injectables are designed and intended for intradermal injection,a novel HA/sorbitol composition containing 2.6% high molecular weight hyaluronic acid stabilized by sorbitol was recently developed to be specifically injected into the adipose tissue to improve the quality of all skin layers,especially the skin ***:The HA/sorbitol composition was investigated versus product comparators in terms of biophysical properties,tolerance in subcutaneous tissue with in vivo implantation study,and skin firmness assessment on human skin ***:The HA/sorbitol composition was characterized by unique and differentiated biophysical properties,proper distribution and high tolerance of the gel composition into the adipose tissue,and the ability to efficiently improve skin ***:The HA/sorbitol composition represents a new attractive solution to treat facial skin aging with an injection strategy specifically targeting the adipose tissue instead of the dermis,to improve the quality of the skin.

关键词： Facial aging hyaluronic acid sorbitol biophysical properties skin quality

Study of biological markers in skin quality treatment by subcutaneous injection of a stabilized composition of 26 mg/mL of high molecular weight HA

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plastic and Aesthetic Research 2022年第1期9卷 539-552页

作者： Jérémie bon bétemps samuel Gavard Molliard basste Hadjab Mhd badi Anas Ghazal Marco Cerrano Research and Development Department Kylane Laboratoires SAPlan-les-Ouates 1228Switzerland Art Clinic Riyadh 12432Saudi Arabia Derma Clinic Jeddah 23432Saudi Arabia Entourage Clinique Lausanne 1003Switzerland

Aim:Hyaluronic acid(HA)injectables have gained rapid acceptance for the treatment of skin rejuvenation.A novel HA/sorbitol composition intended for skin quality improvement containing 2.6%of high molecular weight HA stabilized by sorbitol was recently designed to be injected *** aim of this study was to assess the expression of biological markers of skin quality after administration of the ***:The HA/sorbitol composition was evaluated after injection into the superficial adipose tissue with ex vivo cultured human skin explants versus a product comparator to study the general morphology of the skin tissues and the expression of HA,elastin,collagen type Ⅰ,collagen type Ⅲ,and fibrillin-1 in the dermal ***:The results demonstrate that the HA/sorbitol composition is able to boost the production of HA,elastin,collagen type Ⅰ,collagen type Ⅲ,and fibrillin-1 in the dermis while providing a proper quality of skin ***:The HA/sorbitol composition improved biological markers of skin quality in the dermis after product injection into the superficial adipose *** novel composition can be considered as an attractive solution to treat skin aging by injecting a specific HA/sorbitol formulation to strategically target the subcutaneous tissue to improve the quality of the different layers of the skin.

关键词： Facial aging hyaluronic acid sorbitol skin quality biological markers

Furongian(upper Cambrian) Guole Konservat-Lagersttte from South China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Geologica Sinica(English Edition) 2016年第1期90卷 30-37页

作者： ZHU Xuejian PENG Shanchi samuel ZAMORA bertrand LEFEbVRE CHEN Guiying State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and PalaeontologyChinese Academy of SciencesNanjing 210008China Instituto Geologico y Minero de Espana c/Manuel Lasala 449°B50006 ZaragozaSpain UMR CNRS 5276 Universite Lyon 1&ENS-Lyon69622 VilleurbanneFrance College of Earth Sciences Guilin University of TechnologyGuilin 541004GuangxiChina

The Guole biota contains common shelly fossils and some exceptionally well-preserved softbodied fossils. Stratigraphically, it is located in the mudstones and siltstones of the Sandu Formation near Guole Town, Jingxi County, Guangxi, South China. It is the first Furongian （late Cambrian） burgess Shale-type biota found in the world, thereby filling the gap between middle Cambrian and Lower Ordovician burgess Shale-type Lagerstaitten. Preliminary studies suggest that the Guole biota includes approximately seven metazoan groups as well as algae. These will provide important new evolutionary and ecological information.

关键词： Cambrian Furongian burgess Shale soft-bodied fossils South China

Failure of P-selectin blockade alone to protect the liver from ischemia-reperfusion injury in the isolated blood-perfused rat liver

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第44期14卷 6808-6816页

作者： samuel Wyllie Neal R barshes Saul J Karpen John A Goss Michael E. Debakey Department of Surgery Liver Transplant Center LaboratoryBaylor College of MedicineHoustonTX 77030United States

AIM： To determine if blockade of P-selectin in the isolated blood-perfused cold ex vivo rat liver model protects the liver from ischemia-reperfusion injury. METHODS： The effect of P-selectin blockade was assessed by employing an isolated blood-perfused cold ex vivo rat liver with or without P-selectin antibody treatment before and after 6 h of cold storage in University of Wisconsin solution. RESULTS： In our isolated blood-perfused rat liver model, pre-treatment with P-selectin antibody failed to protect the liver from ischemia-reperfusion injury, as judged by the elevated aspartate aminotransferase activity. In addition, P-selectin antibody treatment did not significantly reduced hepatic polymorphonuclear leukocyte accumulation after 120 min of perfusion. Histological evaluation of liver sections obtained at 120 min of perfusion showed significant oncotic necrosis in liver sections of both ischemic control and P-selectin antibody-treated groups. However, total bile production after 120 rain of perfusion was significantly greater in P-selectin antibody-treated livers, compared to control livers. No significant difference in P-selectin and ICAM-1 mRNAs and proteins, GSH, GSSG, and nuclear NF-kb was found between control and P-selectin antibody-treated livers. CONCLUSION： In conclusion, we have shown that blockade of P-selectin alone failed to reduced polymorphonuclear leukocyte accumulation in the liver and protect hepatocytes from ischemia-reperfusion injury in the isolated blood-perfused cold-ex vivo rat liver model.

关键词： P-selectin Ischemia-reperfusion Antibody-blockade Liver Rat

Is routine measurement of international normalized ratio necessary as part of the investigation of patients with cardiac-type chest pain?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Emergency Medicine 2021年第3期12卷 221-224页

作者： samuel G.Campbell Kirk Magee Ismail Cajee Simon Field Michael b.butler Christine L.Campbell Sarah E.bryson Charles V.Keating Emergency and Trauma Centre Queen Elizabeth II HSCHalifaxNova Scotia B3H 3A7Canada

Dear editor, Chest pain is a frequent complaint of patients presenting to the emergency department (ED), and many of them are referred to the cardiology service for further investigation. At the Charles V. Keating Emergency and Trauma Centre in Halifax, Nova Scotia, Canada, 4,800 (6.6%) of the approximately 73,000 patients per year register with a complaint of “chest pain”, and 20% of patients are referred to cardiology. Coagulation studies, specifi cally international normalized ratio (INR) frequently part of the “routine” panel of blood tests, are ordered for patients in the ED being investigated or treated for chest pain suspected to be cardiac in nature. Recent calls to examine how much of our practice is likely to benefit patients in any way have led us to question the clinical utility of routine use of these tests.

关键词： routine patients pain