An electropenetrography waveform library for the probing and ingestion behaviors of Culex tarsalis on human hands

作     者：Anastasia M.W.Cooper Samuel B.Jameson Victoria Pickens Cameron Osborne Elaine A.Backus Kristopher Silver Dana N.Mitzel 

作者机构：Department of EntomologyKansas State UniversityManhattanKansasUSA Department of Tropical MedicineTulane University School of Public Health and Tropical MedicineNew OrleansLos AngelesUSA USDA Agricultural Research ServiceSan Joaquin Valley Agricultural Sciences CenterParlierCaliforniaUSA National Bio and Agro-Defense FacilityUSDA Agricultural Research ServiceManhattanKansasUSA 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2024年第31卷第4期

页      面：1165-1186页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by the USDA Research,Education,and Economics Workforce Development Agreement(#58-3022-0-002) the Hatch Multistate Project(NE1943) 

主　　题：AC-DC blood feeders electrical penetration graph EPG human hands mosquitoes waveform library 

摘      要：Culex tarsalis Coquillett(Diptera:Culicidae)mosquitoes are capable of vectoring numerous pathogens affecting public and animal ***,the probing behaviors of mosquitoes are poorly understood because they occur in opaque ***(EPG)has the potential to elucidate these behaviors by recording the electrical signals generated during *** used an AC–DC EPG with variable input resistors(Ri levels)to construct a waveform library for *** feeding on human *** events associated with mosquito probing were used to characterize waveforms at four Ri levels and with two electrical current *** optimal settings for EPG recordings of *** probing on human hands was an Ri level of 10^(7)Ohms using an applied signal of 150 millivolts alternating *** for *** included those previously observed and associated with probing behaviors in Aedes aegypti L.(Diptera:Culicidae):waveform families J(surface salivation),K(stylet penetration through the skin),L(types 1 and 2,search for a blood vessel/ingestion site),M(types 1 and 2,ingestion),N(type 1,an unknown behavior which may be a resting and digestion phase),and W(withdrawal).However,we also observed variations in the waveforms not described in ***,which we named types L3,M3,M4,and *** investigation enhances our understanding of mosquito probing *** also provides a new tool for the automated calculation of peak *** work will facilitate future pathogen acquisition and transmission studies and help identify new pest and disease management targets.