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Individual s.ages.of bacterial dichloromethane degradation mapped by carbon and chlorine s.able is.tope analys.s

Journal of Environmental s.iences 2019年第4期31卷 147-160页

作者： Maria L.Torgons.aya Anatoly M.Zyakun Yuri A.Trots.nko Kes.utis.s.Laurinavichius.s.effen Kümmel s.éphane Vuilleumier Hans.H.Richnow Laboratory of Radioactive Is.topes G.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences Laboratory of Mas. s.ectrometry G.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences Department of Is.tope Biogeochemis.ry Helmholtz Centre for Environmental Research-UFZ Univers.té de s.ras.ourg CNRS

The fractionation of carbon and chlorine s.able is.topes.of dichloromethane(CH_2Cl_2,DCM)upon dechlorination by cells.of the aerobic methylotroph Methylobacterium extorquens.DM4 and by purified DCM dehalogenas.s.of the glutathione s.trans.eras. family was.*** effects.for individual s.eps.of the multi-s.age DCM degradation proces.,including trans.er acros. the cell wall from the aqueous.medium to the cell cytoplas.,dehalogenas. binding,and catalytic reaction,were *** obs.rved carbon and chlorine is.tope fractionation accompanying DCM cons.mption by cell s.pens.ons.and enzymes.was.mainly determined by the breaking of C\Cl bonds.and not by inflow of DCM into *** is.tope effects.of DCM dehalogenation were initially mas.ed in high dens.ty cultures.pres.mably due to invers. is.tope effects.of non-s.ecific DCM oxidation under conditions.of oxygen *** cofactor s.pply remarkably affected the correlation of variations.of DCM carbon and chlorine s.able is.topes.Δδ^(13)C/Δδ^(37)Cl),increas.ng corres.onding ratio from 7.2–8.6 to 9.6–10.5 under conditions.of glutathione *** s.gges.s.that enzymatic reaction of DCM with glutathione thiolate may involve s.epwis. breaking and making of bonds.with the carbon atom of DCM,unlike the uncatalyzed reaction,which is.a one-s.age proces.,as.s.own by quantum-chemical modeling.

关键词： Dichloromethane(DCM) Microbial degradation Aerobic methylotrophic bacteria Dichloromethane dehalogenas. Carbon and chlorine is.topic fractionation

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Kinetic s.udy of phytotoxicity induced by foliar lead uptake for vegetables.expos.d to fine particles.and implications.for s.s.ainable urban agriculture

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Journal of Environmental s.iences 2016年第8期28卷 16-27页

作者： TianTian Xiong Annabelle Aus.ruy Antoine Pierart Muhammad s.ahid Eva s.hreck s.éphane Mombo Camille Dumat Univers.té de Toulous. Institut National Polytechnique de Toulouse-Ecole Nationale Supérieure Agronomique de Toulouse (INP-ENSAT) Centre National de la Recherche s.ientifique Ins.itut Ecocitoyen Centre de Vie la Fossette RD 268 Department of Environmental s.iences COMSATS Institute of Information Technology Géos.iences.Environnement Toulous. (GET) Observatoire Midi Pyrénées Université de Toulouse Centre d'Etude et de Recherche Travail Organis.tion Pouvoir (CERTOP) UMR5044 Universite J. Jaurès - Toulouse II

At the global s.ale, foliar metal trans.er occurs.for cons.med vegetables.cultivated in numerous.urban or indus.rial areas.with a polluted atmos.here. However, the kinetics.of metal uptake, trans.ocation and involved phytotoxicity was.never jointly s.udied with vegetables.expos.d to micronic and s.b-micronic particles.PM）. Different leafy vegetables.lettuces.and cabbages. cultivated in RHIZOtes.？ devices.were, therefore, expos.d in a greenhous. for 5, 10 and 15 days.to various.Pb O PM dos.s. The kinetics.of trans.er and phytotoxicity was.as.es.ed in relation to lead concentration and expos.re duration. A s.gnificant Pb accumulation in leaves.up to 7392 mg/kg dry weight（DW） in lettuce） with trans.ocation to roots.was.obs.rved. Lead foliar expos.re res.lted in s.gnificant phytotoxicity, lipid compos.tion change, a decreas. of plant s.oot growth（up to 68.2% in lettuce） and net photos.nthes.s.up to 58% in lettuce）. The phytotoxicity res.lts.indicated plant adaptation to Pb and a higher s.ns.tivity of lettuce in comparis.n with cabbage. Air quality needs. therefore, to be cons.dered for the health and quality of vegetables.grown in polluted areas. s.ch as.certain megacities.in China, Pakis.an, Europe, etc.） and furthermore, to as.es. the health ris.s.as.ociated with their cons.mption.

关键词： Metallic particles.Vegetables.Foliar uptake Trans.er kinetics.and phytotoxicity

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Ionization Proces. of Atoms.by Intens. Femtos.cond Chirped Las.r Puls.s

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Journal of Modern Phys.cs 2013年第10期4卷 20-26页

作者： s.éphane Laulan Jérémie Haché Harouna s. Ba s.mira Barmaki Laboratoire de Phys.que Computationnelle et Photonique Université de Moncton Campus de Shippagan Shippagan Canada

We numerically inves.igate the ionization mechanis. in a real hydrogen atom under intens. fem to s.cond chirped las.r puls.s. The central carrier frequency of the puls.s.is.chos.n to be 6.2 eV (λ = 200 nm), which corres.onds.to the fourth-harmonic of the Ti:s.pphire las.r. Our s.mulation of the las.r-atom interaction cons.s.s.on numerically s.lving the three-dimens.onal time-dependent s.hrodinger equation with a s.ectral method. The unperturbed wave functions.and electronic energies.of the atomic s.s.em were found by us.ng an L2 dis.retization technique bas.d on the expans.on of the wave functions.on B-s.line functions. The pres.nted res.lts.of kinetic energy s.ectra of the emitted electrons.s.ow the s.ns.tivity of the ionization proces. to the chirp parameter. Particular attention is.paid to the important role of the excited bound s.ates.involved in the ionization paths.

关键词： Hydrogen Atom Chirped Las.r Multiphoton Ionization Population Trans.er Nonperturbative Approach

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s.rolimus.eluting s.ent fractures.as.ociated with aneurys. and very late s.ent thrombos.s.in the right coronary artery

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上海医学 2007年第Z1期30卷 226-229页

作者： LIU Xuebo Gary s.Mintz s.éphane G.Carlier Martin B.Leon Department of Cardiology Zhongshan HospitalFudan University Columbia Univers.ty Medical Center and Cardiovas.ular Res.arch Faundation New York

Although the occurrence of coronary s.ent fracture is.rare, recent reports.s.owed that s.ent fracture after s.rolimus.eluting s.ent (s.s. implantation may be as.ociated with neointimal hyperplas.a and res.enos.s. We report two cas.s.of s.ent fracture that occurred late after elective s.s.implantation into the right coronary artery (RCA) that were related to the aneurys., res.enos.s. thrombos.s. and ves.el occlus.on.

关键词： s.ent aneurys. eluting res.enos.s elective implantation dis.al occlus.on longitudinal uns.able

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Comment on “Locating the s.urce field lines.of Jovian decametric radio emis.ions. by YuMing Wang et al.

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Earth and Planetary Phys.cs 2022年第1期6卷 10-12页

作者： Laurent Lamy Baptis.e Cecconi s.éphane Aicardi C.K.Louis LEs.A Observatoire de ParisUniversitéPSLCNRSSorbonne UniversitéUniversitéde Paris5 place Jules Janssen92195 MeudonFrance s.ation de Radioas.ronomie de Nançay Observatoire de ParisUniversitéPSLCNRSUniv.Orléans18330 NançayFrance LAM PythéasAix Marseille UniversitéCNRSCNES38 Rue Frédéric Joliot Curie13013 MarseilleFrance DIO UMS2201 CNRSObservatoire de ParisUniversitéPSL61 avenue de l’Observatoire75014ParisFrance s.hool of Cos.ic Phys.cs DIAS Dunsink ObservatoryDublin Institute for Advanced StudiesDublinIreland

In this.comment on the article“Locating the s.urce field lines.of Jovian decametric radio emis.ions.by Wang YM et al.,2020,we dis.us. the as.umptions.us.d by the authors.to compute the beaming angle of Jupiter’s.decametric emis.ions.induced by the moon *** method,relying on multi-point radio obs.rvations.was.applied to a s.ngle event obs.rved on 14th March 2014 by Wind and both s.EREO A/B s.acecraft from~5 to~16 *** have erroneous.y identified the emis.ion as.a northern(Io-B type)ins.ead of a s.uthern one(Io-D type).We encourage the authors.to update their res.lts.with the correct hemis.here of origin and to tes. their method on a larger s.mple of Jupiter-Io emis.ions.

关键词： planetary magnetos.here Jupiter auroral radio emis.ions planet-moon interaction

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Proteomic and metabolomic profiling underlines.the s.age- and time-dependent effects.of high temperature on grape berry metabolis.

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Journal of Integrative Plant Biology 2020年第8期62卷 1132-1158页

作者： David Lecourieux Chris.ian Kappel s.éphane Claverol Philippe Pieri Regina Feil John E.Lunn Marc Bonneu Lijun Wang Eric Gomès.s.rge Delrot Fatma Lecourieux UMR1287 EGFV INRAEBordeaux Sciences AgroBordeaux UniversityISVV33140 Villenave d'OrnonFrance Ins.itut of Biochemis.ry and Biology Potsdam UniversityD-14476 PotsdamGermany Proteome Platform Bordeaux Functional Genomic CenterBordeaux University33076 BordeauxFrance Max Planck Ins.itute of Molecular Plant Phys.ology 14476 PotsdamGolmGermany Ins.itute of Botany the Chinese Academy of SciencesBeijing 100093China UMR1287 EGFV CNRSINRAEBordeaux Sciences AgroBordeaux UniversityISVV33140 Villenave d'OrnonFrance

Climate change s.enarios.predict an increas. in mean air temperatures.and in the frequency,intens.ty,and length of extreme temperature events.in many wine-growing regions.*** elevated temperature has.detrimental effects.on berry growth and compos.tion,it threatens.the economic and environmental s.s.ainability of wine *** Cabernet s.uvignon fruit-bearing cuttings.we inves.igated the effects.of high temperature(HT)on grapevine berries.through a label-free s.otgun proteomic analys.s.coupled to a complementary metabolomic *** the 2,279 proteins.identified,592 differentially abundant proteins.were found in berries.expos.d to *** gene ontology categories.s.res.,”“protein,”“s.condary metabolis.,”and“cell wall”were predominantly altered under *** temperatures.s.rongly impaired carbohydrate and energy metabolis.,and the effects.depended on the s.age of development and duration of *** amounts.correlated poorly with protein expres.ion levels.in HT berries.highlighting the value of proteomic s.udies.in the context of heat ***,this.work reveals.that HT alters.key proteins.driving berry development and ***,we provide a lis. of differentially abundant proteins.that can be cons.dered as.potential markers.for developing or s.lecting grape varieties.that are better adapted to warmer climates.or extreme heat waves.

关键词： metabolis. extreme high

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Alternative and Eco-Friendly s.nthes.s.of Tetrakis.Aminomethyl)Calix-[4]-Res.rcinarene

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International Journal of Organic Chemis.ry 2017年第4期7卷 403-411页

作者： s. Ali Mous.aoui Ziad Damaj Moheddine Wehbie s.éphane Pellet Ros.aing Iyad Karamé Department of Chemis.ry Laboratory of Catalysis Organometallic and Materiels LCOM Lebanese University Faculty of Sciences I Hadath Lebanon Ins.itut de Chimie s.parative de Marcoule ICSM UMR 5257 - CEA/CNRS/UM2/ENSCM Site de Marcoule Batiment 426 F-30207 Bagnols sur Cèse Cedex France

A cleaner and eco-friendly method was.developed for the preparation of tetrakis.aminomethyl)calix-[4]-res.rcinarene via a s.nthetic pathway of five s.eps.s.arting from methylres.rcinol. This.alternative methodology is.firs.ly bas.d on avoiding the us. of CH2BrCl, which is.a non-eco-friendly s.bs.ance with high ozone depletion potential, and on replacing it by CH2Cl2 as.a readily available reagent with much les. dangerous.effects. s.condly, this.method engages.acetone or CH2Cl2 as.the s.lvent of the bromination s.ep in the place of the very toxic CCl4, leading to tetrakis.bromomethyl)calix-[4]-res.rcinarene. The brominated intermediate has.been reacted with s.dium azide in acetone ins.ead of the high-boiling s.lvent DMs. to produce tetrakis.azidomethyl) calix-[4]-res.rcinarene without the need of tedious.purification. Las.ly, this.work reports.an efficient hydrogenation method of the vers.tile azido adduct employing Pt/C (5%) as.the catalys. for the preparation of the amino cavitand as.an alternative route with high atom economy that can replace the clas.ical methods.us.d currently.

关键词： Bridged Calix--Res.rcinarene Eco-Friendly s.nthes.s Catalytic Hydrogenation Atom Economy Radical s.bs.itution Nucleophilic s.bs.itution CH2Cl2

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Production of liquid bio-fuel from catalytic de-oxygenation:Pyrolys.s.of beech wood and flax s.ives

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燃料化学学报 2019年第2期47卷 153-166页

作者： Chetna Mohabeer Luis.Reyes.Lokmane Abdelouahed s.éphane Marcotte Jean-Chris.ophe Buvat Lucette Tidahy Edmond Abi-Aad Bechara Taouk Normandie Univ INSA Rouen NormandieUNIROUENLaboratoire de Sécurité des Procédés Chimiques Normandie Univ INSA Rouen NormandieUNIROUEN Univers.té du Littoral Cte d'Opale Unité de Chimie Environnementale et Interactions sur le Vivant

This.s.udy pres.nts.a detailed analys.s.of the catalytic de-oxygenation of the liquid and gas.ous.pyrolytic products.of two biomas.es.(beech wood and flax s.ives. us.ng different catalys.s.(commercial HZs.-5 and H-Y,and lab-s.nthes.s.d Fe-HZs.-5,Fe-H-Y,Pt/Al2O3 and CoMo/Al2O3). The experiments.were all conducted in a s.mi-batch reactor under the s.me operating conditions.for all feed materials. BET s.ecific s.rface area,BJH pore s.ze dis.ribution and FT-IR technologies.have been us.d to characteris. the catalys.s.while gas.chromatography-mas. s.ectrometry (GC-Ms.,flame ionis.tion detection (GC-FID) and thermal conductivity detection (GC-TCD) were us.d to examine the liquid and gas.ous.pyrolytic products. It was.firs.ly s.en that at higher catalys.-to-biomas. ratios.of 4∶1,de-oxygenation efficiency did not experience any further s.gnificant improvement. FeHZs.-5 was.deemed to be the mos. efficient of the catalys.s.utilis.d as.it helped reach the lowes. oxygen contents.in the bio-oils.s.mples.and the s.cond bes. was.HZs.-5. It was.als. found that HZs.-5 and H-Y tended to privilege the decarbonylation route(production of CO),whils. their iron-modified counterparts.favoured the decarboxylation one (production of CO2) for both biomas.es.s.udied. It was.then s.en that the major bio-oil components.(carboxylic acids. underwent almos. complete convers.on under catalytic treatment to produce mos.ly unoxygenated aromatic compounds.phenols.and gas.s.like CO and CO2. Finally,phenols.were s.en to be the family mos. s.gnificantly formed from the actions.of all catalys.s.

关键词： pyrolys.s biomas. catalytic treatment de-oxygenation bio-oil upgrading

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Increas.ng the effectivenes. of intracerebral injections.in adult and neonatal mice: a neuros.rgical point of view

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Neuros.ience Bulletin 2015年第6期31卷 685-696页

作者： Bertrand Mathon Mérie Nas.ar Jean s.monnet Caroline Le Duigou s.éphane Clemenceau Richard Miles.Des.emona Fricker Department of Neuros.rgery Groupe Hospitalier Universitaire de La Pitié-SalpêtrièreAPHPF-75013ParisFrance Ins.rm U 1127F-75013ParisFrance CNRs UMR 7225F-75013ParisFrance s.rbonne Univers.tés UPMC Univ Paris 06UMR S 1127F-75013ParisFrance Ins.itut du Cerveau et de la Moelle épinière ICMF-75013ParisFrance

Intracerebral injections.of tracers.or viral cons.ructs.in rodents.are now commonly us.d in the neuros.iences.and mus. be executed perfectly. The purpos. of this.article is.to update exis.ing protocols.for intracerebral injections.in adult and neonatal mice. Our procedure for s.ereotaxic injections.in adult mice allows.the inves.igator to improve the effectivenes. and s.fety, and s.ve time. Furthermore, for the firs. time, we des.ribe a two-handed procedure for intracerebral injections.in neonatal mice that can be performed by a s.ngle operator in a very s.ort time. Our technique us.ng the s.ereotaxic arm allows.a higher precis.on than freehand techniques.previous.y des.ribed. s.ereotaxic injections.in adult mice can be performed in 20 min and have 〉90% efficacy in targeting the injection s.te. Injections.in neonatal mice can be performed in 5 min. Efficacy depends.on the difficulty of precis.ly localizing the injection s.tes. due to the s.all s.ze of the animal. We des.ribe an innovative, effortles., and reproducible s.rgical protocol for intracerebral injections.in adult and neonatal mice.

关键词： rodents s.rgery neuros.iences.tereotaxic injection newborn mice neonatal brain

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s.inal alignment evolution with age:A pros.ective gait analys.s.s.udy

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World Journal of Orthopedics 2017年第3期8卷 256-263页

作者： s.bas.ien Pes.nti Benjamin Blondel Emilie Peltier Elke Viehweger Vincent Pomero Guillaume Authier s.éphane Fuentes.Jean-Luc Jouve Pediatric orthopedics Timone Enfants Aix-Marseille University Ins.itute of Movement s.iences.(CNRs.UMR 7287) Gait Analys.s.Laboratory Hopital Timone Enfants Aix-Marseille University s.ine Unit Hopital de la Timone s.ine unit Timone Aix-Marseille University Neuros.rgery Timone Enfants Aix-Marseille University

AIM To des.ribe,us.ng gait analys.s.the development of s.inal motion in the growing *** Thirty-s.x healthy children aged from 3 to 16 years.old were included in this.s.udy for a gait analys.s.9m-walk).Various.kinematic parameters.were recorded and analyzed s.ch as.thoracic angle(TA),lumbar angle(LA)and s.gittal vertical axis.s.A).The kinetic parameters.were the net reaction moments.N.m/kg)at the thoracolumbar and lumbos.cral *** TA and LA curves.were not s.atis.ically correlated to the age(res.ectively,P=0.32 and P=0.41).s.A increas.d s.gnificantly with age(Ps.in s.gittal plane at the lumbos.cral junction were s.atis.ically correlated to the age(P=0.003),underlining the fact that s.gittal mechanical cons.raints.at the lumbos.cral junction increas. with *** in trans.ers.l plane at the thoracolumbar and lumbos.cral junctions.were s.atis.ically correlated to the age(P=0.0002and P=0.0006),revealing that trans.ers.l mechanical cons.raints.decreas. with *** The kinetic analys.s.s.owed that during growth,a decreas. of tors.onal cons.raint occurs.while an increas. of s.gittal cons.raint is.*** changes.in s.ine biomechanics.are related to the crucial role of the trunk for bipedalis. acquis.tion,allowing s.abilization des.ite lower limbs.*** the acquis.tion of mature gait,the s.ine will mainly undergo cons.raints.in the s.gittal plane.

关键词： s.gittal balance s.ine biomechanics Gait analys.s Thorcic kyphos.s s.ine growth

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