Individual stages of bacterial dichloromethane degradation mapped by carbon and chlorine stable isotope analysis

作     者：Maria L.Torgonskaya Anatoly M.Zyakun Yuri A.Trotsenko Kestutis S.Laurinavichius Steffen Kümmel Stéphane Vuilleumier Hans H.Richnow 

作者机构：Laboratory of Radioactive IsotopesG.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences Laboratory of Mass SpectrometryG.K.Skryabin Institute of Biochemistry and Physiology of MicroorganismsFRC Pushchino Center for Biological ResearchRussian Academy of Sciences Department of Isotope BiogeochemistryHelmholtz Centre for Environmental Research-UFZ Université de StrasbourgCNRS 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2019年第31卷第4期

页      面：147-160页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the Russian Foundation of Basic Research, Russia Nos. 06-04-22000, 12-04-00760, and 15-0404458 the Centre National de la Recherche Scientifique, France CNRS (No. PICS 3380) supported by REALISE, the Alsace Research Network in Environmental Sciences supported by EUEU Marie Curie Host Fellowships (BIOISOTOPE Contract EVK1-CT-2000-56120) 

主　　题：Dichloromethane(DCM) Microbial degradation Aerobic methylotrophic bacteria Dichloromethane dehalogenase Carbon and chlorine isotopic fractionation 

摘      要：The fractionation of carbon and chlorine stable isotopes of dichloromethane(CH_2Cl_2,DCM)upon dechlorination by cells of the aerobic methylotroph Methylobacterium extorquens DM4 and by purified DCM dehalogenases of the glutathione S-transferase family was *** effects for individual steps of the multi-stage DCM degradation process,including transfer across the cell wall from the aqueous medium to the cell cytoplasm,dehalogenase binding,and catalytic reaction,were *** observed carbon and chlorine isotope fractionation accompanying DCM consumption by cell supensions and enzymes was mainly determined by the breaking of C\Cl bonds,and not by inflow of DCM into *** isotope effects of DCM dehalogenation were initially masked in high density cultures,presumably due to inverse isotope effects of non-specific DCM oxidation under conditions of oxygen *** cofactor supply remarkably affected the correlation of variations of DCM carbon and chlorine stable isotopes(Δδ^(13)C/Δδ^(37)Cl),increasing corresponding ratio from 7.2–8.6 to 9.6–10.5 under conditions of glutathione *** suggests that enzymatic reaction of DCM with glutathione thiolate may involve stepwise breaking and making of bonds with the carbon atom of DCM,unlike the uncatalyzed reaction,which is a one-stage process,as shown by quantum-chemical modeling.