Increasing the effectiveness of intracerebral injections in adult and neonatal mice: a neurosurgical point of view

作     者：Bertrand Mathon Mérie Nassar Jean Simonnet Caroline Le Duigou Stéphane Clemenceau Richard Miles Desdemona Fricker 

作者机构：Department of NeurosurgeryGroupe Hospitalier Universitaire de La Pitié-SalpêtrièreAPHPF-75013ParisFrance InsermU 1127F-75013ParisFrance CNRSUMR 7225F-75013ParisFrance Sorbonne UniversitésUPMC Univ Paris 06UMR S 1127F-75013ParisFrance Institut du Cerveau et de la Moelle épinièreICMF-75013ParisFrance 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2015年第31卷第6期

页      面：685-696页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100202[医学-儿科学] 10[医学] 

基　　金：supported by the French Society of Neurosurgery (B.M.) by an ANR Grant JCJC R10206DD (D.F.) 

主　　题：rodents surgery neurosciencesstereotaxic injection newborn mice neonatal brain 

摘      要：Intracerebral injections of tracers or viral constructs in rodents are now commonly used in the neurosciences and must be executed perfectly. The purpose of this article is to update existing protocols for intracerebral injections in adult and neonatal mice. Our procedure for stereotaxic injections in adult mice allows the investigator to improve the effectiveness and safety, and save time. Furthermore, for the first time, we describe a two-handed procedure for intracerebral injections in neonatal mice that can be performed by a single operator in a very short time. Our technique using the stereotaxic arm allows a higher precision than freehand techniques previously described. Stereotaxic injections in adult mice can be performed in 20 min and have 〉90% efficacy in targeting the injection site. Injections in neonatal mice can be performed in 5 min. Efficacy depends on the difficulty of precisely localizing the injection sites, due to the small size of the animal. We describe an innovative, effortless, and reproducible surgical protocol for intracerebral injections in adult and neonatal mice.