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Open Journal of Immunology 2022年第1期12卷 15-39页

作者： Axel Soto-Muñoz rossanna rodríguez-canul Laboratorio de Inmunología y Biología Molecular Departamento de Recursos del Mar Centro de Investigación y de Estudios Avanzados del IPN Mérida México

This study provided new data in the area of cross-reactivity for Pacific white shrimp (Litopenaeus vannamei). Although the cross-reactivity between the most prominent allergenic proteins of shrimp and other crustaceans has been extensively studied, few data are showing the frequency of arthropod-shrimp Litopenaeus vannamei cosensitization in an in silico analysis. A comparative analysis of “typical and non-typical” shrimp L. vannamei allergens with other species was achieved, revealing new allergens and previously characterized shrimp allergens, which validated the comprehensive identification approach used in this study. Importantly, up to 192, amino acid sequences were identified that had matches to shrimp L. vannamei allergens that matched allergenic proteins in mites, insects, fish, bacteria, mammals, birds, and plants.

关键词： Allergens Litopenaeus vannamei Allergenicity Immunoglobulin E Food Allergy

Morphometric Analysis on Shape Transition during Growth of the Red Snapper (<em>Lutjanus campechanus</em>, Poey, 1860)

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Open Journal of Marine Science 2018年第4期8卷 407-430页

作者： Elvia Teresa Mendoza-Barrera María Eugenia Vega-Cendejas Monica Améndola-Pimenta rossanna rodríguez-canul Departamento de Recursos del Mar. Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN)-Unidad Mérida. Carr. Mérida Yucatán México

The red snapper Lutjanus campechanus (Poey, 1860) has a high commercial value that sustains an important fishery in Mexico. In this study, the patterns in morphological variations from early juvenile to adult stages were assessed by geometric methods (GM) in 194 organisms. Changes in shape were more evident and rapid in the early juvenile stage and decreased during adulthood. The principal components analysis of shape (Relative Warp Analysis, or RWA) identified size and body depth as the main sources of variance associated to both juvenile and adult organisms. The outline of the head and the tail showed the most noticeable differences following the ontogenic pathway visualized by thin-plate splines indicating that the ontogenetic pathway of the upper half and the lower half of the dorsal head profile (DHP) are in relatively opposite directions than those from the tail that bends ventrally. The Two-Block Partial Least Square analysis (2B-PLS) and their CR coefficients showed that the two modules had a moderate linear trend (p = 0.001). Although the blocks have morphological changes at different rates, there is a moderate synchrony in growth by modules. This study is the first to report the use of geometrical morphometry in L. campechanus in Mexico.

关键词： Lutjanus campechanus Ontogeny Shape Integration 2B-PLS CR Coefficient

Landscape Analysis for PaV1 Infection in Lobsters Panulirus argus from the Artisanal Fishery of the Eastern Coast of Yucatan, Mexico

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Open Journal of Marine Science 2016年第3期6卷 386-394页

作者： Ruth A. Pérez-Campos Oswaldo Huchim-Lara Silvia Salas María Liceaga-Correa Héctor Hernández-Nuñez Cristina Pascual-Jiménez Pascual-Jiménez rossanna rodríguez-canul Laboratorio de Inmunología y Biología Molecular Mérida Yucatán México Laboratorio de Pesquerías Mérida Yucatán México Laboratorio de Percepción Remota y Sistemas de Información Geográfica Centro de Investigación y de Estudios Avanzados del IPN-Mérida (CINVESTAV-IPN) Merida Mexico Universidad Nacional Autónoma de México Facultad de Ciencias Unidad Académica Sisal Sisal Mexico

Panulirus argus virus 1 (PaV1) is considered a major threat to spiny lobsters Panulirus argus. In this study Geospatial analysis was used to analyze PaV1 distribution in an artisanal fishery of spiny lobster Panulirus argus population from the north coast of the Yucatan Peninsula. Adult and sub-adult P. argus and seabed coverage data were collected from thirty artisanal fishing sites. Five seabed coverage types were identified: seagrass;sand/seagrass mixture;sand only;coral/sand mixture;and seaweed. No juveniles were examined. Of the 358 collected lobsters, PaV1 was identified in four organisms (three sub-adults and one adult) from two fishing sites (termed A & B), both found in a seagrass coverage area. Overall prevalence was of 1.12%. Prevalence was of 20% (2/10) at one site and of 12.6% (2/16) at the other.

关键词： Panulirus argus PaV1 Geospatial Analysis Artisanal Fishery Seagrass

Presence of Infectious Hypodermal and Haematopoietic Necrosis Virus (IHHNV) in Native Shrimps from Southern Mexico

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Open Journal of Marine Science 2017年第3期7卷 424-432页

作者： Ariadne Hernández-Pérez Jesús Alejandro Zamora-Briseñ o Juan Antonio Pérez Vega Richard Juan de Dios Mena-Loria Daniel Coronado-Molina Jorge Hernández-López Norma Angelica-López-Téllez rossanna rodríguez-canul Laboratorio de Inmunología y Biología Molecular Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN) Unidad Mérida Yucatán México Centro de Investigaciones Biológicas del Noroeste Sonora México Centro Regional de Investigación Pesquera de Lerma Campeche Instituto Nacional de la Pesca (CRIP Lerma-INP) Campeche México

A survey for Infectious Hypodermal and Haematopoietic Necrosis Virus (IHHNV) and White Spot Syndrome Virus (WSSV) was performed during two catching seasons of native shrimps in the Yucatan Coast in the Gulf of Mexico. The mtDNA COI barcode identified two endemic species;the southern pink shrimp Penaeus notialis (Pérez Fantante, 1967) and the northern pink shrimp Penaeus brasiliensis (Latreille, 1987) (previously described as Farfantepenaeus brasiliensis). The prevalence of IHHNV was of 18.18% in 2016, and of 8.57% in 2017. All organisms tested negative for WSSV. This is the first identification of wild shrimps in the state of Yucatan Mexico by mtDNA COI barcode as well as the first identification of IHHNV in such species. The presence of IHHNV in wild shrimps populations has a potential of persisting in the coast of Yucatan with putative detrimental effect on local fisheries because once established in natural waters and hosts;such pathogens are almost impossible to eradicate.

关键词： IHHNV WSSV mtDNA COI Barcode Penaeus notialis Penaeus brasiliensis Yucatan Peninsula

Rice Husk at a Glance:From Agro-Industrial to Modern Applications

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Rice science 2024年第1期31卷 14-32页

作者： Masoumeh KORDI Naser FARROKHI Martin I.PECH-canul Asadollah AHMADIKHAH Department of Cell&Molecular Biology Faculty of Life Sciences and BiotechnologyShahid Beheshti UniversityTehran ***Iran 1062 Metallurgical Industry Avenue Industrial Park Saltillo-Ramos ArizpeRamos ArizpeCoahuila 25900Mexico

Excessive waste production has led to the concept of a circular bioeconomy to deliver valuable by-products and improve environmental *** annual worldwide rice production accounts for more than 750 million tons of grain and 150 million tons of *** husk(RH)contains valuable biomaterials with extensive applications in various *** proportions of each component depend primarily on rice genotype,soil chemistry,and climatic *** and its derivatives,including ash,biochar,hydrochar,and activated carbon have been placed foreground of applications in agriculture and other *** the investigation on RH’s compositions,microstructures,and by-products has been done copiously,owing to its unique features,it is still an open-ended area with enormous scope for innovation,research,and ***,we reviewed the latest applications of RH and its derivatives,including fuel and other energy resources,construction materials,pharmacy,medicine,and nanobiotechnology to keep this versatile biomaterial in the spotlight.

关键词： circular bioeconomy rice husk activated carbon rice husk ash rice husk biochar rice husk hydrochar rice husk application

Study of an Ionic Fluid on the Electrochemical Test of A36 Carbon Steel Ingot

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Journal of Materials Science and Chemical Engineering 2022年第2期10卷 29-43页

作者： Socorro Valdez rodriguez Martin Ignacio Pech-canul Hugo Rojas Oscar Sotelo Francisco Javier rodríguez Araceli Espinoza-Vazquez Ociel rodríguez Instituto de Ciencias Físicas-UNAM Cuernavaca Morelos México Centro de investigación y de Estudios Avanzadas del Instituto Politécnico Nacional Civestav-Saltillo Coahuila México Facultad de Ciencias Químicas e Ingeniería-UAEM Morelos México Departamento de Ingeniería Metalúrgica Facultad de Química-UNAM Ciudad de México México

An ionic fluid based on aromatic heterocyclic family constituted by 1,3-diazole groups was investigated. The purpose is to describe their electrochemical characteristics in order to identify the strategy to avoid the A36 carbon steel surface degradation by using electrochemical measurements. We found that the linear polarization resistance reveals an increasing value when the organic unsaturated cyclic ionic fluid was added to the corrosive electrolyte. The polarization curves and Tafel Extrapolation obtained to know the slopes tafel and the inhibitor efficiency from current density (icorr) shows a high efficiency inhibition value.

关键词： A36-Steel Ionic Fluid Electrochemical Degradation Tafel Extrapolation Linear Polarization Resistance Diazole Group Organic Inhibitor

Effect of brazing parameters on fillet size and microstructure of cladded fin-microchannel tube joints

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Transactions of Nonferrous Metals Society of China 2020年第12期30卷 3240-3253页

作者： J.C.GUÍA-TELLO M.I.PECH-canul E.TRUJILLO-VÁZQUEZ M.A.PECH-canul centro de investigación en materiales avanzados(cimav) laboratorio nacional de nanotecnologíamiguel de cervantes no.120chihuahuachi8.31136méxico centro de investigación y de estudios avanzados del instituto politécnico nacional-unidad saltillo av.industria metalúrgica no.1062parque industrial saltillo-ramos arizperamos arizpecoahuila25900méxico conacyt-facultad de ciencias físico matemáticas benemérita universidad autónoma de pueblaav.san claudiocd universitariajardines de san manuelpueblapuebla72570méxico cinvestav-mérida km 6 ant.carr.a progresoap 73 cordemexméridayu3.97310méxico

This work concerns the optimization of furnace brazing conditions for joining micro-multiport aluminum tubes and fins made with AA4343/AA3003/AA4343 brazing sheet in mini-assemblies mimicking the core of an automotive heat *** method was used for design of experiment,considering five process parameters with two levels of *** aim was to maximize the fillet size of the brazed joints,which has an important influence on the thermal integrity and mechanical *** length measurements of brazed joints were performed with a metallographic *** statistical analysis allowed to obtain the optimum values of process parameters(peak temperature,residence time,heating rate,microchannel tube type and flux).At a 95%confidence level,the variability of fillet length is most significantly affected by the peak brazing temperature(77%),residence time(15%)and heating rate(7%).The predicted maximum fillet length was(152±11)μm,which was corroborated by confirmation *** microstructural analysis of tube−fin joints showed that variations in peak temperature and residence time affect only the size of the eutectic zone of fillet formed,but not the nature or composition of the constituent phases.

关键词： brazing cladded fin Taguchi method fillet size microstructure aluminum peak temperature optimization

Evaluation Regarding the Efficacy and Safety of a Probiotic Mixture in Healthy Volunteers with Evacuation Disorders

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Food and Nutrition Sciences 2012年第1期3卷 117-122页

作者： Lourdes rodríguez-Fragoso Adriana Sandoval-Ocampo Martha Corbalá-Nava Carlos A. Arjona-canul Diana Lizbeth Gomez-Galicia Guillermo Tellez Billy Hargis Jorge Reyes-Esparza 不详

Probiotics have been used in humans for almost a century and are widely recommended because they play an important role in gastrointestinal physiological and pathophysiological processes. The aim of this study was to evaluate the effi- cacy and safety of a probiotic mixture in healthy volunteers with evacuation disorders. We undertook a prospective study with historical control. We used the number of evacuations, effort and stool consistency 10 days before, 10 days during and 10 days after treatment with the probiotic as primary outcome measures. Eligible par-ticipants comprised 25 patients with an average age of 25.12 years (range: 18 - 55) who suffered from evacuation disorders. BIO L6 is a func- tional food consisting of a probiotic preparation that contains 1 mg (108 CFUs) of lyophilized strains of Lactic Acid Bacteria resuspended in 250 mL of drinking water. The probiotic preparation was prescribed daily for two weeks, five days a week. A significant increase in the number of evacuations was observed in 23 patients during BIO L6 consump- tion and continued in 20 after consumption. The degree of effort spent defecating decreased in 17 patients during treat- ment and in 7 after treatment. A significant improvement (P < 0.001) in stool consistency was also observed in all pa- tients receiving BIO L6. Fourteen patients had at least one adverse event (AE) for one or two days during treatment;most treatment-related AEs were considered minor or mild in terms of severity and were consistent with AEs reported for probiotic consumption. The results of this study suggest that the use of probiotic mixture BIO L6 is effective, safe and tolerable and provides beneficial effects for subjects with evacuations disorders.

关键词： Chronic Constipation Probiotic Lactic Acid Bacteria

Development and assessment of a multifunctional chitosan-based coating applied on AZ31 magnesium alloy: Corrosion resistance and antibacterial performance against Klebsiella Pneumoniae

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Journal of Magnesium and Alloys 2021年第6期9卷 2133-2144页

作者： Emmanuel Mena-Morcillo Lucien Veleva Mariana Cerda-Zorrilla Montserrat Soria-Castro Juan C.Castro-Alcátara Rosa C.canul-Puc Applied Physics Department Center for Research and Advanced Study(CINVESTAV-IPN)Carr.Ant.a Progreso Km.697310MeridaYucatanMexico Marine Resources Department Center for Research and Advanced Study(CINVESTAV-IPN)Carr.Ant.a Progreso Km.697310MeridaYucatanMexico

This work presents a simple method to functionalise the surface of AZ31 magnesium alloy by applying a duplex MgF_(2)/chitosan coating,which improves its corrosion resistance and provides it with some antibacterial ***,the effect of three chitosan solutions with different concentrations on the growth of the bacteria Klebsiella pneumoniae in nutritive medium(TSB)was evaluated by absorbance kinetics experiments,where the chitosan solution at 2%(m/V)was selected for the coating *** coating application,the AZ31 substrate was pretreated with hydrofluoric acid for 48 hours in order to form a MgF_(2)conversion ***,the coating was applied to the pretreated substrate through the dry-casting *** of the alloy in each surface condition(bare,pretreated,and pretreated+coated with chitosan)were exposed to simulated body fluid(SBF)for 21 days at 37°C,with the solution renewed every 24 hours and the wastes *** surfaces were characterised by SEM-EDS,and XPS after the immersion tests,whereas the stored solutions were employed to measure the change in the Mg-ions *** impedance spectroscopy and potentiodynamic polarisation were performed in each surface condition to compare their corrosion resistance in *** antibacterial activity of the functionalised surfaces was evaluated by the plate counting method and compared with bare *** results were correlated and demonstrate that the modified surface of AZ31 achieved a higher corrosion resistance when it was exposed to SBF,as well as a reduction of the bacterial growth during in vitro tests.

关键词： AZ31 Chitosan coating Simulated body fluid Corrosion Antibacterial activity

Early Detection of White Spot Syndrome Virus(WSSV)in Isolated Hemocytes of Litopenaeus vannamei

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CellBio 2017年第1期6卷 1-12页

作者： Ariadne Hernandez-Perez rossanna rodriguez-canul Edgar Torres-Irineo Fernando Mendoza-Cano Daniel Eduardo Coronado-Molina Jesus Alejandro Zamora-Briseno Jorge Hernandez-Lopez Laboratorio de Inmunología y Biología Molecular Centro de Investigación y de Estudios Avanzados del IPN(CINVESTAV-IPN)Unidad MéridaMéridaMéxico Laboratorio de Pesquerías Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-Unidad MéridaMéridaMéxico UMDI-Sisal Facultad de CienciasUniversidad Nacional Autónoma de MéxicoSierra PapacalMéxico Centro de Investigaciones Biológicas del Noroeste HermosilloMéxico

To date, White Spot Syndrome (WSS) produced by the White Spot Syndrome Virus (WSSV) causes one of the most severe diseases infecting penaeid shrimps worldwide. Although a vast amount of studies has elucidated pathogenesis in live infection models, there is still little information about the interaction of WSSV infections using in vitro models in the whiteleg shrimp Litopenaeus vannamei (L. vannamei) hemocytes. In this study, a WSSV infection kinetics was performed using total hemocytes isolated from healthy L. vannamei organisms and maintained in in vitro conditions using isotonic solution for shrimp (ISS). The infected experimental cells received ≈ 30,000 viral copies of WSSV. The viability of the hemocytes (control and infected group) was measured during the kinetics with trypan blue exclusion method and cells were maintained up to 6 hpi (post-infection) with non-significant differences of viability between both groups. WSSV replication was assessed using RT- PCR at the RNA expression level of the early viral gene Ie1 and transcripts were detected as early as 30 min pi. Hemocytes from WSSV group showed disrupted integrity, degranulation and irregular shape. This study provides evidence of the capability of WSSV to infect and replicates in L. vannamei hemocytes using in vitro assays in short times as 30 min.

关键词： WSSV Litopenaeus vannamei Ie1 Gene Hemocytes In Vitro Infection GLS Analysis