Early Detection of White Spot Syndrome Virus(WSSV)in Isolated Hemocytes of Litopenaeus vannamei

作     者：Ariadne Hernandez-Perez Rossanna Rodriguez-Canul Edgar Torres-Irineo Fernando Mendoza-Cano Daniel Eduardo Coronado-Molina Jesus Alejandro Zamora-Briseno Jorge Hernandez-Lopez 

作者机构：Laboratorio de Inmunología y Biología MolecularCentro de Investigación y de Estudios Avanzados del IPN(CINVESTAV-IPN)Unidad MéridaMéridaMéxico Laboratorio de PesqueríasCentro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-Unidad MéridaMéridaMéxico UMDI-SisalFacultad de CienciasUniversidad Nacional Autónoma de MéxicoSierra PapacalMéxico Centro de Investigaciones Biológicas del NoroesteHermosilloMéxico 

出 版 物：《CellBio》 (细胞生物学（英文）)

年 卷 期：2017年第6卷第1期

页      面：1-12页

学科分类：0908[农学-水产] 09[农学] 

基　　金：funded by the“Laboratorio de Referencia,Analisis y Diagnostico de Sanidad Acuícola del Centro de Investigaciones Biologicas del Noroeste”(#15789) by the Project Conacyt-Ciencia Basica 2013“Actividad antiinflamatoria y cicatrizante del Pepino de Mar(Isostichopus badionotus)en un modelo murino:caracterizacion de la actividad farmacologica y los mecanismos moleculares involucrados”(#221734) 

主　　题：WSSV Litopenaeus vannamei Ie1 Gene Hemocytes In Vitro Infection GLS Analysis 

摘      要：To date, White Spot Syndrome (WSS) produced by the White Spot Syndrome Virus (WSSV) causes one of the most severe diseases infecting penaeid shrimps worldwide. Although a vast amount of studies has elucidated pathogenesis in live infection models, there is still little information about the interaction of WSSV infections using in vitro models in the whiteleg shrimp Litopenaeus vannamei (L. vannamei) hemocytes. In this study, a WSSV infection kinetics was performed using total hemocytes isolated from healthy L. vannamei organisms and maintained in in vitro conditions using isotonic solution for shrimp (ISS). The infected experimental cells received ≈ 30,000 viral copies of WSSV. The viability of the hemocytes (control and infected group) was measured during the kinetics with trypan blue exclusion method and cells were maintained up to 6 hpi (post-infection) with non-significant differences of viability between both groups. WSSV replication was assessed using RT- PCR at the RNA expression level of the early viral gene Ie1 and transcripts were detected as early as 30 min pi. Hemocytes from WSSV group showed disrupted integrity, degranulation and irregular shape. This study provides evidence of the capability of WSSV to infect and replicates in L. vannamei hemocytes using in vitro assays in short times as 30 min.