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Observation of granulations in the basal body of ovarioles and follicular dilatations for the determination of physiological age of Anopheles gambiae s.s.

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Journal of Coastal Life Medicine 2015年第7期 526-530页

作者： rodrigue Anagonou Virgile Gnanguenon Fiacre Agossa Bruno Akinro Armand Akpo Martial Gbegbo Albert Salako Martin Akogbéto Center of Entomological research of Cotonou 06 BP 2604 CotonouBenin Faculty of Sciences and Techniques University of Abomey CalaviCalaviBenin

Objective:To explore ovariole basal body granulations and follicular dilatations for determining physiological age inAnopheles gambiaes.s.(***.s.).Methods:Mosquitoes were collected by using window trap catch and identified *** the first lot ofmosquitoes,they were dissected,and ovary was left in distilled water for reading ovarian tracheoles and the second was cut and transferred to another blade in a physiological liquid for verification of ovariole basal body *** same approach was followed with the second lot of mosquitoes where follicular dilatations were found after classic dilaceration of ovaries were transferred into physiological *** other body parts of mosquitoes were used to identify the species of *** by ***:Among the *** *** the first lot,the method of Detinova determined the age of 89 mosquitoes versus 114 for the observation of granulations(P>0.05).Among the *** *** the second lot,the method of Detinova determined the age of 84 mosquitoes versus 93 for the observation of follicular dilatations(P>0.05).Unlike the method of Detinova,observation of follicular dilatations and basal body granulations of ovarioles were possible beyond the stage II ***:Overall,the observation of follicular dilatations and ovariole basal body granulations are reliable for the determination of the physiological age ***,these two methods can be used beyond the stage II.

关键词： Anopheles gambiae s.s. Physiological age Ovarioles Dilatations Granulations

Multiple Emulsions Able to Be Used for Oral Administration of Active Pharmaceutical Ingredients: Physico-Chemical Parameters Study of Different Phases

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Journal of Modern Physics 2020年第10期11卷 1528-1535页

作者： Louis Augustin Diaga Diouf Alphonse rodrigue Djiboune Mamadou Soumboundou Sidy Mouhamed Dieng Papa Mady Sy Gora Mbaye Mounibé Diarra Pharmaceutical Physics and Biophysics Laboratory Faculty of Medicine Pharmacy and Odontology Cheikh Anta Diop University of Dakar Dakar Senegal Pharmaceutical Physics and Biophysics Laboratory Healthy UFR of Thies University of Thies Thies Senegal Galenic and Industrial Pharmacy Laboratory Healthy UFR of Thies University of Thies Thies Senegal

Multiple emulsions are of great therapeutic interest especially in the administration of medicines which can be inactivated by digestive enzymes;moreover the researches of formulation not being often easy, a control of the different phases physicochemical parameters would be of great interest in rapid formulations and at low cost. When formulating emulsions, the preliminary tests, also known as formulation tests, constitute a step which can be long and expensive because of the quantity of reagents that can be used. A rigorous methodology could thus be of great interest, which is at the aim of our study which consists of evaluating the physico-chemical parameters of different phases used to make thus multiple emulsions. In our study, physico-chemical parameters such as conductivity, pH, density, viscosity, and surface tension have been studied by direct measurement using equipment and also by means of suitable mounting. The results showed that the pH and the surface tension have an important role in the prediction of the stability of emulsions, these latter must be of the same order of magnitude. For all phases conductivity does not have too much interest apart from helping to determine the type of the emulsion.

关键词： Active Pharmaceutical Ingredients Multiple Emulsions Stability Physico-Chemical Parameters

Study of Factors Associated with the Age of Natural Menopause in Menopausal Women Aged 30 to 80 Years from the Keur Massar Health District in 2015 (Senegal)

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Health 2020年第10期12卷 1333-1344页

作者： Fatoumata Binetou Diongue Adama Faye Khadim Niang Jean Augustin Diégane Tine Amadou Ibra Diallo rodrigue Magloire Eyema Anta Tal-Dia Health and Development Institute Cheikh Anta Diop University of Dakar Dakar Senegal

Introduction: Menopause is a biological phenomenon that occurs at a more or less early or late age in the life of every woman. The general objective is to study the factors associated with the age of natural menopause in menopausal women aged between 30 and 80 years who have gone through down Keur Massar health district in 2015. Methodology: This was an observational, cross-sectional, descriptive and analytical study conducted in Keur Massar health district from December 1st to 10th, 2015. Two-stage cluster sampling was conducted. Data were collected during an individual interview at home after consent. They covered socio-economic conditions, health and nutrition status, gynecological-obstetrical history, and lifestyle. A multivariate analysis was performed using the Cox model with a 5% risk of alpha error. Results: A total of 627 postmenopausal women were surveyed. Mean age of these women was 54.5 years (±8.6 years). A proportion of 72.1% of the women was married and 46.7% was in school. Mean age of natural menopause was 47.1 years (±5.03 years). The median age of natural menopause was 47 years. Factors associated with the age of natural menopause were high parity of more than 4 children (Hra = 0.35 [0.13 - 0.92]), the existence of frequent childhood illnesses (Hra = 1.72 [1.13 - 2.64]), use of the intra-uterine device—IUD—as a modern contraceptive method (HRa = 0.39 [0.24 - 0.63]), sibling size (HRa = 0.85 [0.78 - 0.93]), family size (HRa = 1.03 [1.01 - 1.06]), and polygamy regime (HRa = 0.56 [0.37 - 0.85]). Conclusion: Many factors in childhood as well as during the period of female genital activity can impact the age of natural menopause. It is then important to improve the health status of women, children and the standard of living of the population.

关键词： Age Menopause Associated Factors Survival Senegal

Cortical Blindness Following Neonatal Hypoxic Ischaemic Encephalopathy: Cases Series from Bamako

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Open Journal of Ophthalmology 2021年第2期11卷 99-104页

作者： Elien Gagnan Yan Zaou Tou rodrigue Romuald Bakayoko Seydou Sidibe Mohamed Kolé Konikpo Ali Diallo Hammadoun Toure Ousmane Sidibe Tata Institute of African Tropical Ophthalmology University of Sciences and Technology of Bamako Bamako Mali

Purpose: Report of series of cases of cortical blindness that occurred after neonatal hypoxic-ischaemic by analysing its epidemiological frequency in black Africa. Methodology: This is the report of two clinical cases received in consultation on Monday 16th November 2020 and Thursday 7th January 2021 in the paediatric ophthalmology department of the IOTA-University Hospital. Results: They are two infants, aged 05 and 17 months respectively, who were brought in for consultation by their mother for lack of eye-tracking movement since birth. Both infants were born at term following a dystocic delivery. At birth, both infants had a very poor Apar score and were given a resuscitation treatment. The clinical examination coupled with the results of the paraclinical examinations allowed us to conclude at cortical blindness induced the neonatal hypoxic-ischaemic encephalopathy. Their therapeutic management, in collaboration with the neurologist, included the combination of piracetam suspension and Valproate sodium syrup. The evolution after three months of treatment is marked by the regression of epileptic seizures and the perception of light. Conclusion: In black Africa, neonatal hypoxic-ischaemic encephalopathy is the second leading cause of cortical blindness in children, after the neuromalaria sequels.

关键词： Cortical Blindness Neonatal Hypoxic Ischaemic Encepalopathy Bamako

Clinical Presentations and Outcome of Cardiovascular Emergencies in Yaounde: A Cross-Sectional Study

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World Journal of Cardiovascular Diseases 2018年第2期8卷 133-142页

作者： Ba Hamadou Yaya Toure Kalissou Ahmadou Musa Jingi Sylvie Ndongo Amougou Sandrine Edie Dikosso rodrigue Njouoguep Samuel Kingue Department of Internal Medicine and Specialties Faculty of Medicine and Biomedical Sciences University of Yaounde 1 Yaounde Cameroon Internal Medicine Service Cardiology Unit Yaounde Central Hospital Yaounde Cameroon Yaounde Emergency Center Yaounde Cameroon

Background: Cardiovascular emergencies have become a public health problem with a high burden in low-income settings. This is due to the high rates of cardiovascular risk factors that are fast reaching epidemic?proportions. There is paucity of data on cardiovascular emergencies to guide repost strategies in our setting. Our aim was to determine the clinical presentation and outcome of cardiovascular emergencies at Yaounde Emergency Center.?Methods: We carried out this cross-sectional study between June 2015 and May 2017. We included all patients with confirmed cardiovascular emergency. We consecutively collected data on socio-demography, symptoms on admission, past history, clinical findings, and final diagnosis at discharge or in the event of death. Results: Of the 8285 patients admitted for medical emergencies, 388 (4.7%) were cardiovascular emergencies. Their mean age was 59.5 ± 13.8 years, and 59% were males. The Medical Emergency Aid Service was the means of transporting 4% of patients. The median time of arrival at the hospital was 48 hours. Symptoms on admission were mainly weakness of a limb (43.8%), and altered consciousness (33.5%). The most common cardiovascular emergencies were ischemic stroke (30.9%), hypertensive emergency (21.4%), and hemorrhagic stroke (16.5%). The most common comorbidity was diabetes (21.9%). The death rate in the 24 - 72 hours was 14.4%. The causes of death were hypertensive emergency (35.7%), and hemorrhagic stroke (30.3%). Conclusion: Stroke and hypertensive emergency were the most frequent cardiovascular emergencies. The early mortality was high. Hemorrhagic stroke and hypertensive emergencies accounted for most cases of death.

关键词： Cardiovascular Emergencies Ischemic Stroke Hemorrhagic Stroke Cameroon

Mineral Fertilization Influences the Acceptability of Fresh Pulp and Juice Made from Sugarloaf Pineapple

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Agricultural Sciences 2020年第3期11卷 342-353页

作者： Sabai Kate Elvire Line Sossa Codjo Emile Agbangba rodrigue Idohou Edmond Sacla Aide Gbènoukpo Pierre Tovihoudji Brice Sinsin Institut National des Recherches Agricoles du Benin CotonouBenin Laboratoire des Sciences du Sol Département de Production VégétaleUniversitéd’Abomey-CalaviCotonouBenin Laboratoire de Biomathématiques et d’Estimations Forestières Universitéd’Abomey-CalaviCotonouBénin Laboratoire de Recherche en Biologie Appliquée Ecole Polytechnique d’Abomey-CalaviUniversitéd’Abomey-CalaviCotonou Benin Ecole de Gestion et de Production Végétale et Semencière Université Nationale d’AgricultureKetouBenin Laboratory of Hydraulics and Environmental Modeling(HydroModE-Lab) Facultéd’AgronomieUniversitéde ParakouParakouBenin Laboratoire d’Ecologie Appliquée Universitéd’Abomey-CalaviCotonouBénin

Fertilization practices can influence the quality of pineapple fruit and consequently its acceptability by consumers who are increasingly oriented towards high quality agri-food products. This study aimed at evaluating the influence of N, P and K on some physico-chemical and organoleptic characteristics of pineapple (Ananas comosus L. Merr) for fresh consumption and juice processing. A complete NPK factorial design was installed in the south Benin. The treatments NPK in g plant-1 were randomized into four blocks: T1;T2;T3;T4;T5;T6;T7;T8;T9. Brix juice was determined using a refractometer and pH with a pH meter. An evaluation of sensory characteristics such as sweetness, acid taste and aroma of fresh pineapple pulp and processed juice was made by a panel of tasters selected and trained. A principal components analysis, followed by a numerical classification was performed on fruits’ sensory and physicochemical characteristics. Results showed that juice pH was significantly influenced by the phosphorus and potassium. Potassium influenced significantly juice yield. Some physico-chemical characteristics and sensory attributes were correlated between them and groups of treatments are formed for both the juice and the fresh fruit.

关键词： Ananas comosus Fertilizer Fruit Intensification Juice Sensory

Hydro-Sedimentary Dynamics of the Grand-Popo Lagoon Located Upstream of the Mobile Embouchure “Bouche du Roy” in South-Western Benin

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Journal of Geoscience and Environment Protection 2021年第11期9卷 98-112页

作者： Honorin Gbinibou Andemi Gérard A. F. d’Almeida Moussa Bio Djara Léandre Accalogoun rodrigue A. Adechina Christophe Kaki Laboratory of Geology Mines & Environment (Labo GME) Department of Earth Sciences (DES) University of Abomey-Calavi Cotonou Benin ACL Consultant: General Engineering Design Office Cotonou Benin

The problems of flooding, bank erosion and even the breaching of the barrier beach by the Grand-Popo lagoon are a concern in the south-western Beninese river-lagoon complex. The present study aims to improve the knowledge of the factors controlling the hydro-sedimentary functioning of this lagoon of Grand-Popo. It was based on bathymetric and sedimentological studies and the analysis of physical-chemical parameters of the water and allowed to know the morphology of the lagoon bottom and the distribution of sedimentary facies according to the intensity of the water current. The salinity of the lagoon waters shows a west-east gradient passing thus from 0‰ to 0.78‰ in the main direction of flow. Over the whole lagoon system, the average liquid flows vary from -38.499 to 159.13 m3/s. The bathymetry indicates depths varying from 0.4 to 6.5 m, revealing type V and type U lagoon bottoms which reflect acute bottom concavities marked by hollowing under the effect of strong currents or by sandy terraces resulting from the continuous input of sediments carried by the Mono River. These sediments are heterogeneously distributed in the lagoon bottom and vary from free sand to mud. Given the complexity of the factors that control the hydro-sedimentary functioning of the Grand-Popo lagoon, it is recommended that periodic hydrological and bathymetric monitoring be carried out to locate exceptional variations in water levels to prevent not only the risks of overflowing lagoon waters and the flooding that follows but also spectacular erosion of the lagoon banks.

关键词： Grand-Popo Lagoon Hydro-Sedimentary Functioning Lagoon Bottoms Sediments

实用鸡病鉴别诊断指南--第6篇痛风/肾衰竭

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国外畜牧学（猪与禽） 2016年第3期36卷 37-38页

作者：蔡霞 Inês rodrigue Luca Vandi Simone Schaumberger 百奥明新加坡公司百奥明控股有限公司

禽痛风是肾脏损伤引起的,多种原因可导致尿酸和尿酸盐在肾小管、心脏浆膜、肝脏、肠系膜、气囊或腹膜中沉积。由于其病原学很复杂,因此很难诊断。但最常见的症状是脱水、鸡冠发白、抑郁以及双爪红肿,家禽运动受影响。禽痛风主要在蛋鸡... 详细信息

禽痛风是肾脏损伤引起的,多种原因可导致尿酸和尿酸盐在肾小管、心脏浆膜、肝脏、肠系膜、气囊或腹膜中沉积。由于其病原学很复杂,因此很难诊断。但最常见的症状是脱水、鸡冠发白、抑郁以及双爪红肿,家禽运动受影响。禽痛风主要在蛋鸡中发生,可导致50%以上的死亡率,19~35周龄鸡群最易患此病。该病的原因众多（表1）,可从管理、营养相关到病原体以及饲料中的霉菌毒素。

关键词：鸡冠发尿酸盐结晶鸡病肾脏损伤磷平衡鉴别诊断菌毒素可从传染性法氏囊病传染性支气管炎

Autologous Adipose-Derived Mesenchymal Stem Cells Embedded in Platelet-Rich Fibrin in Diabetic Foot Ulcers

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Open Journal of Regenerative Medicine 2021年第2期10卷 19-30页

作者： Charbel Khalil Diana Chaker Rawad Salameh Yvonne-Elise Germanos Elie El Kayem Fady Nader Jessica Habbouche rodrigue Chemaly Albert Azar Ahmad Ibrahim Reviva Stem Cell Platform for Research and Applications Center Bsalim Lebanon Middle East Institute of Health University Hospital Bsalim Lebanon Faculty of Pharmacy Saint Joseph University Beirut Lebanon National Institute of Health and Medical Research INSERM Paris Sud France Faculty of Pharmacy Therapeutic Innovation from Fundamentals to Applications Paris Saclay University Ch&#228 tenay Malabry France Faculty of Medicine Lebanese University Beirut Lebanon School of Medicine Lebanese American University Beirut Lebanon Michigan Medicine Speciality Pharmacy Ann Arbor MI USA Cancer Center Middle East Institute of Health University Hospital Bsalim Lebanon

Context and Aim: Mesenchymal stem cells (MSCs) and platelet-rich fibrin (PRF) have emerged as ideal candidates for advanced therapies of various therapeutically-challenging diseases;however, their regenerative potential in diabetic foot ulcers (DFU) has not been well determined. In this study, we reviewed our clinical experience in mitigating chronic ulcer complications of diabetic foot through a conventional treatment of autologous adipose-derived MSCs embedded in PRF with pure PRF injections. Materials and Methods: The present study was carried out in 10 patients with an open DFU wound selected over a period of 1 year starting from April 2019. Patients were either injected with PRF alone (Group A) or injected with MSCs derived from adipose tissue (ADMSC) embedded in (PRF (Group B). Results: Patients in Group B had a better healing index when compared to Group A. Conclusion: Use of ADMSC embedded in PRF showed promising results to treat DFU.

关键词： Diabetic Foot Ulcer Diabetes Mesenchymal Stem Cells Adipose Tissue Platelet Rich Fibrin