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Observation of granulations in the basal body of ovarioles and follicular dilatations for the determination of physiological age of Anopheles gambiae s.s.

Observation of granulations in the basal body of ovarioles and follicular dilatations for the determination of physiological age ofAnopheles gambiaes.s.

作     者:Rodrigue Anagonou Virgile Gnanguenon Fiacre Agossa Bruno Akinro Armand Akpo Martial Gbegbo Albert Salako Martin Akogbéto 

作者机构:Center of Entomological research of Cotonou06 BP 2604 CotonouBenin Faculty of Sciences and TechniquesUniversity of Abomey CalaviCalaviBenin 

出 版 物:《Journal of Coastal Life Medicine》 (海岸生命医学杂志(英文版))

年 卷 期:2015年第7期

页      面:526-530页

学科分类:08[工学] 0812[工学-计算机科学与技术(可授工学、理学学位)] 

基  金:Supported by Bill&Melinda GATES Foundation and the Ministry of Higher Education and Scientific Research of Benin 

主  题:Anopheles gambiae s.s. Physiological age Ovarioles Dilatations Granulations 

摘      要:Objective:To explore ovariole basal body granulations and follicular dilatations for determining physiological age inAnopheles gambiaes.s.(***.s.).Methods:Mosquitoes were collected by using window trap catch and identified *** the first lot ofmosquitoes,they were dissected,and ovary was left in distilled water for reading ovarian tracheoles and the second was cut and transferred to another blade in a physiological liquid for verification of ovariole basal body *** same approach was followed with the second lot of mosquitoes where follicular dilatations were found after classic dilaceration of ovaries were transferred into physiological *** other body parts of mosquitoes were used to identify the species of *** by ***:Among the *** *** the first lot,the method of Detinova determined the age of 89 mosquitoes versus 114 for the observation of granulations(P0.05).Among the *** *** the second lot,the method of Detinova determined the age of 84 mosquitoes versus 93 for the observation of follicular dilatations(P0.05).Unlike the method of Detinova,observation of follicular dilatations and basal body granulations of ovarioles were possible beyond the stage II ***:Overall,the observation of follicular dilatations and ovariole basal body granulations are reliable for the determination of the physiological age ***,these two methods can be used beyond the stage II.

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