检索结果-南通市图书馆

DNA Damage,Apoptosis and C-myc,C-fos,and C-jun Overexpression Induced by Selenium in Rat Hepatocytes

Biomedical and Environmental Sciences 2006年第3期19卷 197-204页

作者： ri-an yu cheng-feng yang xue-min chen Department of Occupational and Environmental Health MOE Key Laboratory of Environment and Health School of Public Health Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei China

Objective To study the effects of selenium on DNA damage, apoptosis and c-myc, c-fos, and c-jun expression in rat hepatocytes. Methods Sodium selenite at the doses of 5, 10, and 20 μmol/kg was given to rats by i.p. and there were 5 male SD rats in each group. Hepatocellular DNA damage was detected by single cell gel electrophoresis （or comet assay）. Hepatocellular apoptosis was determined by TUNEL （TdT-mediated dUTP nick end labelling） and flow cytometry. C-myc, c-fos, and c-jun expression in rat bepatocytes were assayed by Northern dot hybridization. C-myc, c-fos, and c-jun protein were detected by immunohistochemical method. Results At the doses of 5, 10, and 20μmol/kg, DNA damage was induced by sodium selenite in rat hepatocytes and the rates of comet cells were 34.40%, 74.80%, and 91.40% respectively. Results also showed an obvious dose-response relationship between the rates of comet cells and the doses of sodium selenite （r=0.9501, P〈0.01）. Sodium selenite at the doses of 5, 10, and 20μmol/kg caused c-myc, c-fos, and c-jun overexpression obviously. The positive brown-yellow signal for proteins of c-myc, c-fos, and c-jun was mainly located in the cytoplasm of bepatocytes with immunohistocbemical method. TUNEL-positive cells were detected in selenium-treated rat livers. Apoptotic rates （%） of selenium-treated liver cells at the doses of 5, 10, and 20 μmol/kg were （3.72±1.76）, （5.82±1.42）, and （11.76±1.87） respectively, being much higher than those in the control. Besides an obvious dose-response relationship between apoptotic rates and the doses of sodium selenite （r=0.9897, P〈0.01）, these results displayed a close relationship between DNA damage rates and apoptotic rates, and the relative coefficient was 0.9021, P〈0.01. Conclusion Selenium at 5-20μmol/kg can induce DNA damage, apoptosis, and overexpression of c-myc, c-fos, and c-jun in rat hepatocytes.

关键词： Selenium DNA damage Apoptosis C-myc C-fos C-jun

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A 2.5D finite element approach for predicting ground vibrations generated by vertical track irregularities

引用

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2011年第12期12卷 885-894页

作者： xue-cheng BIAN Chang CHAO Wan-feng JIN yun-min chen MOE Key Laboratory of Soft Soils and Geoenvironmental Engineering Department of Civil Engineering Zhejiang University Hangzhou 310058 China

Dynamic responses of track structure and wave propagation in nearby ground vibration become significant when train operates on high speeds. A train-track-ground dynamic interaction analysis model based on the 2.5D finite element method is developed for the prediction of ground vibrations due to vertical track irregularities. The one-quarter car mode,1 is used to represent the train as lumped masses connected by springs. The embankment and the underlying ground are modeled by the 2.5D finite element approach to improve the computation efficiency. The Fourier transform is applied in the direction of train＇s movement to express the wave motion with a wave-number. The one-quarter car model is coupled into the global stiffness matrix describing the track-ground dynamic system with the displacement compatibility condition at the wheel-rail interface, including the irregularities on the track surface. Dynamic responses of the track and ground due to train＇s moving loads are obtained in the wave-number domain by solving the governing equation, using a conventional finite element procedure. The amplitude and wavelength are identified as two major parameters describing track irregularities. The irregularity amplitude has a direct impact on the vertical response for low-speed trains, both for short wavelength and long wavelength irregularities. Track irregularity with shorter wavelength can generate stronger track vibration both for low-speed and high-speed cases. For low-speed case, vibrations induced by track irregularities dominate far field responses. For high-speed case, the wavelength of track irregularities has very little effect on ground vibration at distances far from track center, and train＇s wheel axle weights becomes dominant.

关键词： High-speed railway 2.5D finite element method One-quarter car model Ground vibration Track irregularities

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Preparation and efficacy evaluation of Paenibacillus polymyxa KM2501-1 microbial organic fertilizer against root-knot nematodes

引用

Journal of Integrative Agriculture 2022年第2期21卷 542-551页

作者： cheng Wan-li ZENG Li yang xue HUANG Dian yu Hao chen Wen CAI min-min ZHENG Long-yu yu Zi-niu ZHANG Ji-bin State Key Laboratory of Agricultural Microbiology/National Engineering Research Center of Microbial Pesticides/College of Life Science and Technology Huazhong Agricultural UniversityWuhan 430070P.R.China

Root-knot nematodes(RKNs) cause huge yield losses to agricultural crops worldwide. Meanwhile, livestock manure is often improperly managed by farmers, which leads to serious environmental pollution. To resolve these two problems, this study developed a procedure for the conversion of chicken manure to organic fertilizer by larvae of Hermetia illucens L. and Bacillus subtilis BSF-CL. Chicken manure organic fertilizer was then mixed thoroughly with Paenibacillus polymyxa KM2501-1 to a final concentration of 1.5×10^(8)CFU g^(-1). The efficacy of KM2501-1 microbial organic fertilizer in controlling root-knot nematodes was evaluated in pot and field experiments. In pot experiments, applying KM2501-1 microbial organic fertilizer either as a base fertilizer or as a fumigant at the dose of 40 g/pot suppressed root-knot disease by 61.76 and 69.05% compared to the corresponding control treatments, respectively. When applied as a fumigant at the dose of 1 kg m;in field experiments, KM2501-1 microbial organic fertilizer enhanced the growth of tomato plants, suppressed root-knot disease by 49.97%, and reduced second stage juveniles of RKN in soil by 88.68%. KM2501-1 microbial organic fertilizer controlled RKNs better than commercial bio-organic fertilizer in both pot and field experiments. These results demonstrate that this co-conversion process efficiently transforms chicken manure into high value-added larvae biomass and KM2501-1 microbial organic fertilizer with potential application as a novel nematode control agent.

关键词： chicken manure Hermetia illucens L. microbial organic fertilizer root-knot nematode field experiment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Oxidation of Difluorocarbene by Pyridine N-Oxide and Ensuing Access to Carbamoyl Fluorides

引用

Chinese Journal of Chemistry 2023年第22期41卷 2981-2987页

作者： Hai-Jun Tang xue-min Shi Xiao-yu Zhu cheng-Qiang Wang Chao feng Technical Institute of Fluorochemistry(TiF) Institute of Advanced Synthesis(iAs)State Key Laboratory of Material-Oriented Chemical EngineeringSchool of Chemistry and Moiecular EngineeringNanjing TechUniversity30 South Puzhu RoadNanjingJiangsu211816China

A practical one-pot preparation of carbamoyl fluorides from easily obtained pyridine N-oxide,commercially available secondary amines and synthetically versatile difluorocarbene precursors(Ruppert-Prakash reagent or chen's reagent)was developed herein,which dexterously resorted to the oxidation of difluorocarbene by external pyridine N-oxide to produce the toxic and gaseous fluorophosgene in *** features of this method include nice functionality tolerance,late-stage modification of drug molecules and the recoveryand recycle of quinoline.

关键词： Difluorocarbene Pyridine N-oxide Fluorophosgene Carbamoyl fluorides Amines Fluorine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of nerve growth factor on the action potential duration and repolarizing currents in a rabbit model of myocardial infarction

引用

Journal of Geriatric Cardiology 2013年第1期10卷 39-51页

作者： yun-feng Lan Jian-cheng Zhang Jin-Lao Gao xue-Ping Wang Zhou Fang Yi-cheng Fu Mei-Yan chen min Lin Qiao xue yang Li Institute of Geriatric Cardiology of Chinese PLA General Hospital 28 Fuxing Road 100853 Beijing China Provincial Clinical Medicine College of Fujian Medical University Fujian 350001 China

Objectives To investigate the effect of nerve growth factor (NGF) on the action potential and potassium currents of non-infarcted myocardium in the myocardial infarcted rabbit *** Rabbitswith occlusion of the left anterior descending coronary arterywere prepared and allowed to recover for eight weeks (healed myocardial infarction, HMI). During ligation surgery of the left coronary artery, a polyethylene tube was placed near the left stellate ganglion in the subcutis of the neck for the purpose of administering NGF 400 U/d for eight weeks (HMI + NGF group). Cardiomyocytes were isolated from regions of the non-infarcted left ventricular wall and the action potentials and ion currents in these cellswere recorded using whole-cell patch clamps. Results Comparedwith HMI and control cardiomyocytes, significant prolongation of APD50 or APD90 (Action potential duration (APD) measured at 50% and 90% of repolarization) in HMI + NGF cardiomyocytes was found. The results showed that the 4-aminopyridine sensitive transient outward potassium current (Ito), the rapidly activated omponent of delayed rectifier potassium current (IKr), the slowly activated component of delayed rectifier potassium current (IKs), and the L-type calcium current (ICaL) were significantly altered in NGF + HMI cardiomyocytes compared with HMI and control cells. Conclusions Our results suggest thatNGF treatment significantly prolongsAPDinHMI cardiomyocytes and that a decrease in outward potassium currents and an increase of inward Ca2+ current are likely the underlying mechanism of action.

关键词： Action potential Cardiomyocytes Myocardial infarction Nervous growth factor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Decomposition of pre-existing Au-P clusters induced in situ a-Fe grains uniformization in Fe-P-based nanocrystalline alloys

引用

Rare Metals 2024年第10期43卷 5242-5255页

作者： min-Hao Han cheng Sun Guo-Guo Xi yang Meng Qiang Luo xue-Qian yu Wen-feng Zhang Hao Liu Hong-Jie Xu Tao Zhang Key Laboratory of Aerospace Materials and Performance(ministry of Education) School of Materials Science and EngineeringBeihang UniversityBeijing 100191China Laboratory of Functional Fiber Materials Institute of Bast Fiber CropsChinese Academy of Agricultural ScienceChangsha 410205China School of Materials Science and Engineering Jiangsu Key Laboratory for Advanced Metallic MaterialsSoutheast UniversityNanjing 211189China Institute for Advanced Materials North China Electric Power UniversityBeijing 102206China Center for Advanced Analysis and Computational Science Zhengzhou UniversityZhengzhou 450001China

In Fe-based amorphous-/nanocrystalline ribbons,the uniformization and refinement of a-Fe grains are key aspects for optimizing their soft magnetic and mechanical ***,the Fe-P-C-B nanocrystalline alloy system was selected for *** produced as-spun ribbons with pre-existing nanocrystals through melt-quenching and then obtained a well-distributed a-Fe nanocrystalline structure through annealing below the first crystallization onset temperature(633 K)resulting in excellent magnetic properties(saturation magnetization of 1.65 T and coercivity of 1.6 A·m^(-1))ultra-wide annealing temperature window(from 613 to 733 K),and extremely high annealing stability(up to 480 min at 633 K).Furthermore,we propose a new in situ two-step mechanism for the uniformization of a-Fe nanocrystals,which is separately induced by the decomposition of the Au-P clusters and the pre-existing a-Fe nanocrystals during *** work underscores the crucial significance of micro-alloying via metastable clusters primarily influenced by metal-phosphide interactions in the process of refining a-Fe ***,i introduces a new principle for optimizing the comprehensive properties of Fe-based amorphous/nanocrystalline alloys.

关键词： Fe-based nanocrystalline alloys Soft magnetic property In-situ nano-crystallization Microalloying

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Isothermal oxidation behavior of a new Re-free nickel-based single-crystal superalloy at 950℃

引用

Rare Metals 2017年第8期36卷 617-621页

作者： xue-feng Zhou Guang chen Ya-Ya feng Zhi-Xiang Qi min-Zhi Wang Pei Li Jia-Lin cheng Engineering Research Center of Materials Behavior and Design ministry of Education Nanjing University of Science and TechnologyNanjing 210094China School of Chemistry and Materials Engineering Changshu Institute of TechnologyChangshu 215500China School of Materials Science and Engineering Nanjing Instituteof TechnologyNanjing 211167China

The isothermal oxidation behavior of a new Refree nickel-based single-crystal superalloy in air at 950 ℃ for 200 h was studied by scanning electron microscopy（SEM）with energy-dispersive spectroscopy（EDS）and X-ray diffraction（XRD）.The results indicate that oxidation kinetics obeys parabolic law approximately,and the mass gain increases rapidly during initial oxidation stage and then gradually slows *** oxidation scales are composed of three layers：the outer layer mainly consists of NiO with a small amount of CoO;the intermediate layer is mainly composed of Cr_2O_3 with a small amount of spinel compounds such as CrTaO_4,NiCr_2O_4,CoCrAl_2O_4,CoAl_2O_4,and NiAl_2O_4;and the inner layer is composed of Al_2O_*** Al_2O_3 layer suppresses the diffusion of elements between oxygen and alloy elements,slows down the alloy oxidation speed,and also suppresses the growth of the oxide scale and reduces the oxidation rate,which is agreeable with the oxidation kinetics.

关键词： Nickel-based single-crystal superalloy Isothermal oxidation Oxidation kinetic Surfacemorphology

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

In-situ physical/chemical cross-linked hydrogel electrolyte achieving ultra-stable zinc anode-electrolyte interface towards dendrite-free zinc ion battery

引用

Journal of Energy Chemistry 2024年第10期97卷 342-351,I0007页

作者： chen-yang Li Jiang-Lin Wang Dong-Ting Zhang min-Peng Li Hao chen Wei-Hai Yi Xin-Ying Ren Bao Liu xue-feng Lu Mao-cheng Liu State Key Laboratory of Advanced Processing and Recycling of Non-ferrous Metals Lanzhou University of TechnologyLanzhou 730050GansuChina School of Materials Science and Engineering Lanzhou University of TechnologyLanzhou 730050GansuChina Chaowei Power Group Co. LTDChangxing 313100ZhejiangChina School of Vehicle Engineering Jiangsu UniversityNanjing 210031jiangsuChina

Hydrogen evolution reaction(HER),zinc corrosion,and dendrites growth on zinc metal anode are the major issues limiting the practical applications of zinc-ion ***,an in-situ physical/chemical cross-linked hydrogel electrolyte(carrageenan/polyacrylamide/ZnSO_(4),denoted as CPZ)has been developed to stabilize the zinc anode-electrolyte interface,which can eliminate side reactions and prevent dendrites *** in-situ CPZ hydrogel electrolyte improves the reversibility of zinc anode due to eliminating side reactions caused by active water ***,the electrostatic interaction between the SO_(4)^(-)groups in CPZ and Zn^(2+)can encourage the preferential deposition of zinc atoms on(002)crystal plane,which achieve dendrite-free and homogeneous zinc *** in-situ hydrogel electrolyte offers a streamlined approach to battery manufacturing by allowing for direct integration into the ***,the Zn//Zn half battery with CPZ hydrogel electrolyte can enable an ultra-long cycle over 5500 h at a current density of 0.5 mA cm^(-2),and the Zn//Cu half battery reach an average coulombic efficiency of 99.37%.The Zn//V_(2)O_5-GO full battery with CPZ hydrogel electrolyte demonstrates94.5%of capacity retention after 2100 *** study is expected to open new thought for the development of commercial hydrogel electrolytes for low-cost and long-life zinc-ion batteries.

关键词： In-suit CPZ hydrogel electrolyte Hydrogen evolution reaction and zinc corrosion Dendrites growth Zinc anode-electrolyte interface Zn ion batteries

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mobilisation of endothelial progenitor cells： one of the possible mechanisms involved in the chronic administration of melatonin preventing erectile dysfunction in diabetic rats

引用

Asian Journal of Andrology 2012年第3期14卷 481-486,I0009页

作者： xue-feng Qiu Xiao-Xin Li yun chen Hao-cheng Lin Wen yu Run Wang yu-Tian Dai Department of Urology Affiliated Drum Tower HospitalSchool of MedicineNanjing UniversityNanjing 210008China Department of Urology Nantong Hospital of Traditional Chinese MedicineNantong 226001China Department of Urology University of Texas Medical School at Houston and M.D.Anderson Cancer CenterHoustonTexas 77030USA

Diabetes-induced oxidative stress plays a critical role in the mobilisation of endothelial progenitor cells （EPCs） from the bone marrow to the circulation. This study was designed to explore the effects of chronic melatonin administration on the promotion of the mobilisation of EPCs and on the preservation of erectile function in type I diabetic rats. Melatonin was administered to streptozotocin-induced type I diabetic rats. EPCs levels were determined using flow cytometry. Oxidative stress in the bone marrow was indicated by the levels of superoxide dismutase and malondialdehyde. Erectile function was evaluated by measuring the intracavernous pressure during an electrostimulation of the cavernous nerve. The density of the endothelium and the proportions of smooth muscle and collagen in the corpus cavernosum were determined by immunohistochemistry. The administration of melatonin increased the superoxide dismutase level and decreased the malondialdehyde level in the bone marrow. This effect was accompanied by an increased level of circulating EPCs in the diabetic rats. The intracavernous pressure to mean arterial pressure ratio of the rats in the treatment group was significantly greater, compared with diabetic control rats. The histological analysis demonstrated an increase in the endothelial density of the corpus cavernosum after the administration of melatonin. However, melatonin treatment did not change the proportions of smooth muscle and collagen in the corpus cavernosum of diabetic rats. Chronic administration of melatonin has a beneficial effect on preventing erectile dysfunction （ED） in type I diabetic rats. Promoting the mobilisation of EPCs is one of the possible mechanisms involved in the improvement of ED.

关键词： diabetes endothelial progenitor cells erectile dysfunction melatonin mobilisation oxidative stress

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

LB100 ameliorates nonalcoholic fatty liver disease via the AMPK/Sirt1 pathway

引用

World Journal of Gastroenterology 2019年第45期25卷 6607-6618页

作者： xue-yang chen Chang-Zhou Cai Meng-Li yu Ze-min feng yu-Wei Zhang Pei-Hao Liu Hang Zeng Chao-Hui yu Department of Gastroenterology The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvinceChina Clinical Research Center for Hepatobiliary and Pancreatic Diseases of Zhejiang Province Hangzhou 310003Zhejiang ProvinceChina

BACKGROUND It is well known that nonalcoholic fatty liver disease(NAFLD)is associated with insulin resistance(IR).LB100,a serine/threonine protein phosphatase 2A(PP2A)inhibitor,is closely related to ***,there is little data regarding its direct influence on *** To elucidate the effect and underlying mechanism of LB100 in *** After 10 wk of high fat diet(HFD)feeding,male C57BL/6 mice were injected intraperitoneally with vehicle or LB100 for an additional 6 wk(three times a week).The L02 cell line was treated with LB100 and free fatty acids(FFAs)for 24 *** and eosin and oil red O staining were performed for histological *** blot analysis was used to detect the protein expression of Sirtuin 1(Sirt1),total and phosphorylated AMP-activated protein kinaseα(AMPKα),and the proteins involved in lipogenesis and fatty acid *** mRNA levels were determined by *** inhibition of AMPK was performed to further examine the exact mechanism of LB100 in *** LB100 significantly ameliorated HFD-induced obesity,hepatic lipid accumulation and hepatic injury in *** addition,LB100 significantly downregulated the protein levels of acetyl-CoA carboxylase,sterol regulatory element-binding protein 1 and its lipogenesis target genes,including stearoyl-CoA desaturase-1 and fatty acid synthase,and upregulated the levels of proteins involved in fatty acidβ-oxidation,such as peroxisome proliferator-activated receptorα(PPARα),peroxisome proliferator-activated receptor gamma coactivator-1α(PGC-1α),carnitine palmitoyltransferase 1α,acyl-CoA oxidase 1 and uncoupling protein 2,as well as the upstream mediators Sirt1 and AMPKαin the livers of HFD-fed *** vitro,LB100 alleviated FFA-induced lipid accumulation in L02 cells through the AMPK/Sirt1 signaling *** studies showed that the curative effect of LB100 on lipid accumulation was abolished by inhibiting AMPKαin L02 *** PP2A inhibition by LB

关键词： LB100 Nonalcoholic fatty liver disease Serine/threonine-protein phosphatase 2A Lipid metabolism AMP-activated protein kinaseα Sirtuin 1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：