Preparation and efficacy evaluation of Paenibacillus polymyxa KM2501-1 microbial organic fertilizer against root-knot nematodes

作     者：CHENG Wan-li ZENG Li YANG Xue HUANG Dian YU Hao CHEN Wen CAI Min-min ZHENG Long-yu YU Zi-niu ZHANG Ji-bin CHENG Wan-li;ZENG Li;YANG Xue;HUANG Dian;YU Hao;CHEN Wen;CAI Min-min;ZHENG Long-yu;YU Zi-niu;ZHANG Ji-bin

作者机构：State Key Laboratory of Agricultural Microbiology/National Engineering Research Center of Microbial Pesticides/College of Life Science and TechnologyHuazhong Agricultural UniversityWuhan 430070P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第2期

页      面：542-551页

核心收录：

学科分类：090403[农学-农药学(可授农学、理学学位）] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0904[农学-植物保护] 

基　　金：supported by the project from China Ocean Mineral Resources R&D Association(COMRA DY135-B2-17) the National Key Technologies R&D Program of China(2018YFD0500203 and 2018YFF0213503) the Major Projects of Technological Innovation in Hubei Province,China(2017ABA072) 

主　　题：chicken manure Hermetia illucens L. microbial organic fertilizer root-knot nematode field experiment 

摘      要：Root-knot nematodes(RKNs) cause huge yield losses to agricultural crops worldwide. Meanwhile, livestock manure is often improperly managed by farmers, which leads to serious environmental pollution. To resolve these two problems, this study developed a procedure for the conversion of chicken manure to organic fertilizer by larvae of Hermetia illucens L. and Bacillus subtilis BSF-CL. Chicken manure organic fertilizer was then mixed thoroughly with Paenibacillus polymyxa KM2501-1 to a final concentration of 1.5×10^(8)CFU g^(-1). The efficacy of KM2501-1 microbial organic fertilizer in controlling root-knot nematodes was evaluated in pot and field experiments. In pot experiments, applying KM2501-1 microbial organic fertilizer either as a base fertilizer or as a fumigant at the dose of 40 g/pot suppressed root-knot disease by 61.76 and 69.05% compared to the corresponding control treatments, respectively. When applied as a fumigant at the dose of 1 kg m;in field experiments, KM2501-1 microbial organic fertilizer enhanced the growth of tomato plants, suppressed root-knot disease by 49.97%, and reduced second stage juveniles of RKN in soil by 88.68%. KM2501-1 microbial organic fertilizer controlled RKNs better than commercial bio-organic fertilizer in both pot and field experiments. These results demonstrate that this co-conversion process efficiently transforms chicken manure into high value-added larvae biomass and KM2501-1 microbial organic fertilizer with potential application as a novel nematode control agent.