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clinical, cellular, microscopic, and ultrastructural studies of a case of fibrogenesis imperfecta ossium

Bone Research 2017年第2期5卷 123-136页

作者： Melissa L Barron Mark S Rybchyn Sutharshani Ramesh rebecca S Mason S Fiona Bonar Paul Stalley Sundeep Khosla Bernie Hudson christopher Arthur Edward Kim Roderick J clifton-Bligh Phillip B clifton-Bligh Department of Physiology School of Medical Sciences Bosch Institute University of Sydney Sydney 2006 New South Wales Australia Douglas HanlyMoir Pathology Macquarie Park 2113 New South Wales Australia Department of Orthopaedics Royal Prince Alfred HospitalCamperdown 2050 New South Wales Australia Department of Endocrinology Mayo Clinic Rochester 55905 MN USA Department of Microbiology Royal North Shore Hospital St Leonards 2065 New South Wales Australia Department ofMicrobiology Royal North Shore Hospital St Leonards 2065 New South Wales Australia Department of Haematology Royal North Shore Hospital St Leonards 2065 New South Wales Australia Department of Endocrinology Royal North Shore Hospital St Leonards 2065 New SouthWales Australia and Faculty of Medicine University of Sydney Sydney 2006 New South Wales Australia

Fibrogenesis imperfecta ossium is a rare disorder of bone usually characterized by marked osteopenia and associated with variable osteoporosis and osteosclerosis, changing over time. Histological examination shows that newly formed collagen is abnormal, lacking birefringence when examined by polarized light. The case presented demonstrates these features and, in addition, a previously undocumented finding of a persistent marked reduction of the serum c3 and c4. Osteoblasts established in culture from a bone biopsy showed abnormal morphology on electron microscopy and increased proliferation when cultured with benzoylbenzoyl-ATP and 1,25-dihydroxyvitamin D, contrasting with findings in normal osteoblasts in culture. A gene microarray study showed marked upregulation of the messenger RNA(mRNA) for G-protein-coupled receptor 128(GPR 128), an orphan receptor of unknown function and also of osteoprotegerin in the patient's osteoblasts in culture. When normal osteoblasts were cultured with the patient's serum, there was marked upregulation of the mRNA for aquaporin 1. A single pathogenetic factor to account for the features of this disorder has not been defined, but the unique findings described here may facilitate more definitive investigation of the abnormal bone cell function.

关键词： ultrastructural cellular clinical

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High affinity soluble ILT2 receptor:a potent inhibitor of cD8^(+)T cell activation

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Protein & cell 2010年第12期1卷 1118-1127页

作者： Ruth K.Moysey Yi Li Samantha J.Paston Emma E.Baston Malkit S.Sami Brian J.cameron Jessie Gavarret Penio Todorov Annelise Vuidepot Steven M.Dunn Nicholas J.Pumphrey Katherine J.Adams Fang Yuan rebecca E.Dennis Deborah H.Sutton Andy D.Johnson Joanna E.Brewer rebecca Ashfield Nikolai M.Lissin Bent K.Jakobsen Immunocore Limited 57c Milton ParkAbingdonOxonOX144RXUK current address:Oxford Nanopore Technologies UK current address:Geneva Merck Serono S.A. Switzerland current address:Adaptimmune Limited UK

Using directed mutagenesis and phage display on a soluble fragment of the human immunoglobulin superfamily receptor ILT2(synonyms:LIR1,MIR7,cD85j),we have selected a range of mutants with binding affinities enhanced by up to 168,000-fold towards the conserved region of major histocompatibility complex(MHc)class I *** in a dimeric form,either by chemical cross-linking with bivalent polyethylene glycol(PEG)derivatives or as a genetic fusion with human IgG Fc-fragment,the mutants exhibited a further increase in ligand-binding strength due to the avidity effect,with resident half-times(t1/2)on the surface of MHc I-positive cells of many *** novel compounds antagonized the interaction of cD8 co-receptor with MHc I in vitro without affecting the peptide-specific binding of T-cell receptors(TcRs).In both cytokine-release assays and cell-killing experiments the engineered receptors inhibited the activation of cD8^(+)cytotoxic T lymphocytes(cTLs)in the presence of their target cells,with subnanomolar potency and in a dose-dependent *** a selective inhibitor of cD8^(+)cTL responses,the engineered high affinity ILT2 receptor presents a new tool for studying the activation mechanism of different subsets of cTLs and could have potential for the development of novel autoimmunity therapies.

关键词： cD8^(+)T cells cellular activation autoimmunity cell surface molecules binding affinity phage display

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Improvements to a Novel Device Simulating Pneumatic Intussusception Reduction

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Open Journal of Radiology 2013年第2期3卷 73-77页

作者： Steffan K. Soosman Joseph c. Li Glade E. Roper Anthony S. Wexler rebecca Stein-Wexler Thomas Ray Sanchez Department of Mechanical and Aerospace Engineering University of California at Davis Civil and Environmental Engineering Land Air and Water Resources Davis USA Department of Radiology University of California at Davis Sacramento USA John A. Burns School of Medicine University of Hawaii Honolulu USA School of Medicine University of California at Davis Sacramento USA

Objectives: Intussusception, an uncommon but important cause of acute abdomen during infancy and early childhood, can be safely and effectively treated by fluoroscopic air reduction. Although pediatric radiology fellows report ample opportunity to practice intussusception reduction, radiology residents report few opportunities to participate in this procedure. Adequate training to reduce intussusception is essential, as successful reduction obviates the need for surgery. A teaching device that simulates intussusception reduction could help radiology residents develop the skills necessary to perform this procedure. Materials and Methods: We report on improvements made to a training device developed in 2010 by Stein-Wexler et al. Since then we have simplified the manufacturing process, added several unique patient cases, improved the software so that adding patient scenarios is easier, and improved the graphic interface to make the simulation more realistic and facilitate feedback. Results: The simulator is now a customizable and robust standalone package. A thorough instructor’s manual and improvements to the graphic design-such as embedded checklists, built-in feedback mechanisms, and a more intuitive interface-make the simulator easier to use. conclusion: We have improved our previously-reported teaching device for intussusception reduction and produced a robust simulator. We plan to make this device available to programs that train physicians in pneumatic intussusception reduction.

关键词： Intussusception Reduction Simulator Teaching

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Studies on Different Hydrolysis Procedures for Drugs of Abuse in Hair

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chinese chemical Letters 1999年第3期10卷 239-242页

作者： Ma, c Zhou, TH chinese Acad Med Sci Inst Mat Med Beijing 100050 Peoples R China Peking Union Med coll Beijing 100050 Peoples R China

Different hydrolysis and extraction procedures have been studied for the determination of drugs of abuse in hair samples. The results showed that acid and enzymatic hydrolysis gave high extraction recoveries for 6-monoacetylmorphine, morphine, codeine and heroin. Enzymatic hydrolysis was more expensive. After alkaline hydrolysis, 6-monoacetylmorphine, a unique proof of heroin abuse, could not be detected and after methanolic extraction, the extract was dirty and the obtained chromatogram showed an interfering background. It is concluded that acid hydrolysis is the extraction method of choice.

关键词： drugs of abuse hydrolysis hair

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轮作草地土壤碳蓄积与矿化动态特征

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草地学报 2019年第4期27卷 1037-1043页

作者：林栋张德罡 Mcculley rebecca L. 周生伟甘肃农业大学草业学院甘肃兰州730070 美国肯塔基大学植物与土壤科学系肯塔基列克星敦40546-0091 甘肃农业大学理学院甘肃兰州730070

为探明作物-草地轮作系统中草地土壤碳(carbon,c)蓄积与矿化动态特征,本试验以温带气候区有机农牧场低投入蔬菜生产后的5年草地轮作土壤为研究对象,测定不同轮作年限和季节土壤有机碳(Soil Organic carbon,SOc)及其颗粒有机碳(Particula... 详细信息

为探明作物-草地轮作系统中草地土壤碳(carbon,c)蓄积与矿化动态特征,本试验以温带气候区有机农牧场低投入蔬菜生产后的5年草地轮作土壤为研究对象,测定不同轮作年限和季节土壤有机碳(Soil Organic carbon,SOc)及其颗粒有机碳(Particulate Organic carbon,POc)和非颗粒有机碳(non-Particulate Organic carbon,n-POc)组分含量,并对土壤c矿化潜力进行分析。结果表明,草地轮作年限显著影响SOc含量(Pc含量无显著影响;SOc含量从草地建植初期的17.31 g·kg^-1增加到第5年的20.88 g·kg^-1,且接近永久性草地SOc含量;POc敏感指示土壤有机c库变化动态,草地生长年限显著影响土壤POc和n-POc组分变化(Pc含量增加了53.5%,n-POc含量增加17.6%,春季草地土壤n-POc含量显著高于夏季和冬季;草地轮作4年后土壤c矿化率显著升高,并接近永久性草地水平;秋季草地土壤在71 d的培养期内平均c矿化速率为5.9μg·g^-1·d^-1,显著低于其他季节。草地轮作增强了土壤c矿化潜力,提高了土壤c循环强度和有机c有效性,同时延长了SOc半衰期,对增加土壤c库容量和稳定性具有重要作用。

关键词：碳蓄积颗粒有机碳碳矿化轮作草地

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X-ray Multiple Diffraction Topographic Imaging Technique For Growth History Study of Habit Modifying Impurity Doped crystals

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chemical Research in chinese Universities 2004年第4期20卷 411-416页

作者： LAI X. MA c. K. J. Robert M. c. Miller Institute of Particle Science and Engineering Department of Chemical Engineering University of LeedsInstitute of Particle Science and Engineering Department of Chemical Engineering University of LeedsInstitute of Particle Science and Engineering Department of Chemical Engineering University of LeedsCCLRC Daresbury Laboratory Daresbury Leeds LS2 9JT UKLeeds LS2 9JT UKLeeds LS2 9JT UKWarrington Cheshire WA4 4AD UK

A novel crystal characterization instrument has been built up in which a combination of X-ray multiple diffraction and X-ray topography is applied to enabling the cross-correlation between micro-crystallographic symmetry and its spatial dependence in relation to lattice defects. This facility is used to examine, in a self-consistent manner, growth sector-dependant changes to both the crystallographic structure and the lattice defects associated with the action of habit-modifying additives in a number of representative crystal growth systems. In addition, the new instrument can be used to probe micro-crystallographic aspects(such as distortion to crystal symmetry) and relate these in a spatially resolved manner to the crystal defect structure in crystals doped with known habit modifiers.

关键词： X-ray diffraction X-ray multiple diffraction X-ray topography Habit modification crystal growth Growth defects

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Identification of anticancer drugs to radiosensitise BRAF-wild-type and mutant colorectal cancer

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cancer Biology & Medicine 2019年第2期16卷 234-246页

作者： rebecca carter Azadeh cheraghchi-Bashi Adam Westhorpe Sheng Yu Yasmin Shanneik Elena Seraia Djamila Ouaret Yasuhiro Inoue catherine Koch Jenny Wilding Daniel Ebner Anderson J. Ryan Francesca M. Buffa Ricky A. Sharma NIHR University college London Hospitals Biomedical Research centre/ UcL cancer Institute University College London London WC1E 6DD UK NIHR Oxford Biomedical Research centre Department of Oncology University of Oxford Oxford 0X1 2JD UK computational Biology and Integrative Genomics University of Oxford Oxford 0X1 2JD UK NDM Research Building Nuffield Department of Medicine University of Oxford Oxford 0X1 2JD UK Weatherall Institute of Molecular Medicine John Radcliffe Hospital University of Oxford Oxford 0X1 2JD UK Mie University Graduate School of Medicine Department of Gastrointestinal and Pediatric Surgery Division of Reparative Medicine Institute of Life Sciences Edobashi 2-174 Tsu Japan Departme nt of Biology Massachusetts In stitute of Tech no logy Cambridge Massachusetts 02142 USA Target Discovery Institute National Phenotypic Screening Centre Nuffield Department of Medicine University of OxfordOxford 0X1 2JD UK cRUK & MRc Oxford Institute for Radiation Oncology University of Oxford Oxford 0X1 2JD UK

Objective: Patients with BRAF-mutant colorectal cancer(cRc) have a poor prognosis. Molecular status is not currently used to select which drug to use in combination with radiotherapy. Our aim was to identify drugs that radiosensitise cRc cells with known BRAF ***: We screened 298 oncological drugs with and without ionising radiation in colorectal cancer cells isogenic for BRAF. Hits from rank product analysis were validated in a 16-cell line panel of human cRc cell lines, using clonogenic survival assays and xenograft models in ***: Most consistently identified hits were drugs targeting cell growth/proliferation or DNA damage repair. The most effective class of drugs that radiosensitised wild-type and mutant cell lines was PARP inhibitors. In clonogenic survival assays, talazoparib produced a radiation enhancement ratio of 1.9 in DLD1(BRAF-wildtype) cells and 1.8 in RKO(BRAF V600 E) cells. In DLD1 xenografts, talazoparib significantly increased the inhibitory effect of radiation on tumour growth(P ≤ 0.01).conclusions: Our method for screening large drug libraries for radiosensitisation has identified PARP inhibitors as promising radiosensitisers of colorectal cancer cells with wild-type and mutant BRAF backgrounds.

关键词： Radiosensitizer colorectal cancer PARP inhibitor radiotherapy

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Th1 cytokines promote T-cell binding to antigen-presenting cells via enhanced hyaluronan production and accumulation at the immune synapse

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cellular & Molecular Immunology 2010年第3期7卷 211-220页

作者： Paul L Bollyky Stephen P Evanko rebecca P Wu Susan Potter-Perigo S Alice Long Brian Kinsella Helena Reijonen Kelly Guebtner Brandon Teng christina K chan Kathy R Braun John A Gebe Gerald T Nepom Thomas N Wight Benaroya Research Institute SeattleWAUSA

Hyaluronan(HA)production by dendritic cells(Dcs)is known to promote antigen presentation and to augment T-cell activation and *** hypothesized that pericellular HA can function as intercellular‘glue’directly mediating T cell–Dc *** primary human cells,we observed HA-dependent binding between T cells and Dcs,which was abrogated upon pre-treatment of the Dcs with 4-methylumbelliferone(4-MU),an agent which blocks HA ***,T cells regulate HA production by Dcs via T cell-derived cytokines in a T helper(Th)subset-specific manner,as demonstrated by the observation that cell-culture supernatants from Th1 but not Th2 clones promote HA *** effects were seen upon the addition of exogenous Th1 cytokines,IL-2,interferon c(IFN-c)and tumor necrosis factor a(TNF-a).The critical factors which determined the extent of Dc–T cell binding in this system were the nature of the pre-treatment the Dcs received and their capacity to synthesize HA,as T-cell clones which were pre-treated with monensin,added to block cytokine secretion,bound equivalently irrespective of their Th *** data support the existence of a feedforward loop wherein T-cell cytokines influence Dc production of HA,which in turn affects the extent of Dc–T cell *** also document the presence of focal deposits of HA at the immune synapse between T-cells and APc and on dendritic processes thought to be important in antigen *** data point to a pivotal role for HA in Dc–T cell interactions at the IS.

关键词： dendritic cell hyaluronan immune synapse pericellular matrix Th1

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Genetic characterization of melon accessions in the U.S. National Plant Germplasm System and construction of a melon core collection

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Molecular Horticulture 2021年第1期1卷 72-84页

作者： Xin Wang Kaori Ando Shan Wu Umesh K.Reddy Prabin Tamang Kan Bao Sue A.Hammar rebecca Grumet James D.Mccreight Zhangjun Fei Boyce Thompson Institute Cornell UniversityIthacaNY 14853USA U.S.Department of Agriculture-Agricultural Research Service Crop Improvement and Protection Research UnitSalinasCA 93905USA Nunhems USA IncAcampoCA 95220USA Gus R.Douglass Institute and Department of Biology West Virginia State UniversityInstituteWV 25112USA U.S.Department of Agriculture-Agricultural Research Service Natural Products Utilization Research UnitThad Cochran Research CenterP.O.Box 1848OxfordMS 38677USA Department of Horticulture Michigan State UniversityEast LansingMI 48824USA U.S.Department of Agriculture-Agricultural Research Service Robert W.Holley Center for Agriculture and HealthIthacaNY 14853USA.

Melon(*** L.)is an economically important vegetable crop cultivated *** melon collection in the *** Plant Germplasm System(NPGS)is a valuable resource to conserve natural genetic diversity and provide novel traits for melon *** we use the genotyping-by-sequencing(GBS)technology to characterize 2083 melon accessions in the NPGS collected from major melon production areas as well as regions where primitive melons *** structure and genetic diversity analyses suggested that *** *** was firstly introduced from the centers of origin,Indian and Pakistan,to central and West Asia,and then brought to Europe and *** *** from East Asia was likely derived from *** *** in India and Pakistan and displayed a distinct genetic background compared to the rest of *** accessions from other geographic *** developed a core collection of 383 accessions capturing more than 98%of genetic variation in the germplasm,providing a publicly accessible collection for future research and genomics-assisted breeding of ***-five morphological characters investigated in the core collection indicated high variability of these characters across accessions in the ***-wide association studies using the core collection panel identified potentially associated genome regions related to fruit quality and other horticultural *** study provides insights into melon origin and domestication,and the constructed core collection and identified genome loci potentially associated with important traits provide valuable resources for future melon research and breeding.

关键词： Melon c.melo Germplasm Genetic characterization core collection Genome-wide association study

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探索MOOc教学方法在mLearning中的运用

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中国远程教育 2012年第5期 23-29页

作者： Inge DE Waard Apostolos Koutropoulos Nilgün zdamar Keskin Sean c.Abajian rebecca Hogue c.Osvaldo Rodriguez Michael Sean Gallagher 何伏刚马东明孙海民比利时热带医学研究所美国麻省大学波士顿校区土耳其阿纳多卢大学美国加州州立大学北岭分校加拿大渥太华大学阿根廷拉普拉塔国立大学英国爱丁堡大学北京师范大学教育学部中国人民公安大学信息安全工程系河北民族师范学院网络信息中心

本文把大型开放式网络课程(Massively Open Online course,以下简称MOOc)作为适应移动学习(mLearning)的一种可能的教学方法,这种教学方法建立在与当前学习或教学形式相适宜的基础上。本文呈现了一个MobiMOOc应用的案例研究,该案例使用M... 详细信息

本文把大型开放式网络课程(Massively Open Online course,以下简称MOOc)作为适应移动学习(mLearning)的一种可能的教学方法,这种教学方法建立在与当前学习或教学形式相适宜的基础上。本文呈现了一个MobiMOOc应用的案例研究,该案例使用MOOc形式创建一门课程,研究展示了MOOc和mLearning之间的协同特性,并将当前数字化的协作学习和知识建构这两个理想领域结合在一起。MobiMOOc是聚焦于mlearning、为期六周的网上课程,于2011年的4月到5月之间进行。MobiMOOc是免费开放的课程,有556位学习者加入了课程谷歌群组。课程结束时的调查结果有力地证明了MOOcs和mLearning之间的协同作用。本篇论文对时间和地点的独立性、情境学习、协作学习、潜在的跨学科信息交流,以及应用于网上学习的技术,如社交媒体,移动设备等方面进行了探讨。

关键词：移动学习大型开放式网络课程(MOOc) 联通主义协作学习开放教育资源

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