检索结果-南通市图书馆

Current Inj.ction Provokes Rapid Expansion of the Guard Cell Cytosolic Volume and Triggers Ca^2＋ Signals

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2016年第3期9卷 471-480页

作者： Lena j. Voss rainer Hedrich M. Rob G. Roelfsema Molecular Plant Physiology and Biophysics Julius-von-Sachs Institute for Biosciences Biocenter Werzburg University Julius-von-Sachs-Platz 2 97082 Werzburg Germany

High-resolution microscopy opens the door for detailed single-cell studies with fluorescent reporter dyes and proteins. We used a confocal spinning disc microscope to monitor fluorescent dyes and the fluorescent protein Venus in tobacco and Arabidopsis guard cells. Multi-barreled microelectrodes were used to inj.ct dyes and apply voltage pulses, which provoke transient rises in the cytosolic Ca^2＋ level. Voltage pulses also caused changes in the distribution of Lucifer Yellow and Venus, which pointed to a reversible increase of guard cell cytosolic volume. The dynamic cytosolic volume changes turned out to be provoked by current inj.ction of ions. A reduction of the clamp current, by blocking K^＋ uptake channels with Cs^＋, strongly suppressed the cytosolic volume changes. Cs^＋ not only inhibited the expansion of the cytosol, but also inhibited hyperpolarization-induced elevations of the cytosolic Ca^2＋ concentration. A complete loss of voltage-induced Ca^2＋ signals occurred when Ca^2＋-permeable plasma membrane channels were simultaneously blocked with La^3＋. This shows that two mechanisms cause hyperpolarization-induced elevation of the cytosolic Ca^2＋-concentration：（i） activation of voltage-dependent Ca^2＋-permeable channels, （ii） osmotically induced expansion of the cytosol, which leads to a release of Ca^2＋ from intracellular stores.

关键词： guard cell cytosolic Ca^2+ cytosolic volume voltage clamp current inj.ction ion channel

Viscoelasticity and shear resistance at the aggregate scale of structured and organic carbon-free Chernozems

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2023年第5期33卷 754-764页

作者： Christoph HAAS rainer HORN Philipp SAGGAU rainer DUTTMANN Institute for Plant Nutrition and Soil Science University of KielKiel 24118Germany Department of Geography Division of Landscape Ecology and Geoinformation SciencesUniversity of KielKiel 24118Germany

Soil rheology characterises the flow behaviour of soils at the particle-particle to aggregate *** sweep tests(ASTs)are often the method of choice for parameterizing soil flow properties,such as the shear strain values at the end of the linear viscoelastic range(i.e.,the deformation is mainly elastic)and at the yield point(i.e.,elastic equals plastic deformation).Samples from seven soil profiles and five soil depths of Chernozems,collected in the Maidanetske study area,close to Uman City of Ukraine,were analysed to evaluate the effect of soil organic carbon(SOC)on the parameters related to soil microstructural stability derived from *** organic carbon was removed with H_(2)O_(2)to determine the soil texture-dependent values of soil rheological properties,which were compared to the values determined for samples with intact water-stable *** shear resistance-related parameters increased for aggregated soil samples compared to SOC-free soil samples,indicating an increase in soil stability due to *** contrast,the values of the overall viscoelasticity and the shear strain were reduced for aggregated soil samples,indicating decreased soil *** functions were applied to predict the shear strain-dependent loss and storage moduli and shear stress values as a function of SOC *** particles(630–2000μm)and volumetric water content improved the *** conclude that increased SOC content,through the gluing and cementing effects of SOC and altered aggregate shapes compared to SOC-free soil materials,contributes to higher microstructural ***,the increased water content in the SOC-containing soil samples reversed soil strengthening *** was due to the fact that a more rapid increase in positive water pressure under shear stress weakened the samples and the spherical aggregates began to rotate more easily,thus loosing energy,when compared to platy particles of the SOC-free soil materials.

关键词： aggregation rheology shear strain shear stress soil organic carbon depletion soil stability

Conditional Generative Adversarial Networks for modelling fuel sprays

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Energy and AI 2023年第2期12卷 62-75页

作者： Cihan Ates Farhad Karwan Max Okraschevski rainer Koch Hans-j.rg Bauer Karlsruhe Institute of Technology(KIT) Institute of Thermal TurbomachineryGermany Karlsruhe Institute of Technology(KIT) Machine Intelligence in Energy SystemsGermany

In this study, the probabilistic, data driven nature of the generative adversarial neural networks (GANs)was utilized to conduct virtual spray simulations for conditions relevant to aero engine combustors. Themodel consists of two sub-modules: (i) an autoencoder converting the variable length droplet traj.ctories intofixed length, lower dimensional representations and (ii) a Wasserstein GAN that learns to mimic the latentrepresentations of the evaporating droplets along their lifetime. The GAN module was also conditioned withthe inj.ction location and the diameters of the droplets to increase the generalizability of the whole *** training data was provided from highly resolved 3D, transient Eulerian–Lagrangian, large eddy simulationsconducted with OpenFOAM. Neural network models were created and trained within the open source machinelearning framework of PyTorch. Predictive capabilities of the proposed method was discussed with respect tospray statistics and evaporation dynamics. Results show that conditioned GAN models offer a great potentialas low order model approximations with high computational efficiency. Nonetheless, the capabilities of theautoencoder module to preserve local dependencies should be improved to realize this potential. For the currentcase study, the custom model architecture was capable of conducting the simulation in the order of secondsafter a day of training, which had taken one week on HPC with the conventional CFD approach for the samenumber of droplets (200,000 traj.ctories).

关键词： Generative Adversarial Networks Generative learning Fuel inj.ction Aero engines Multivariate time series

Cellular heterogeneity in pancreatic cancer: the different faces of gremlin action

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第11期7卷 3992-3994页

作者： Aristidis Moustakas j.Matthias Löhr rainer L.Heuchel Department of Medical Biochemistry and Microbiology Uppsala UniversityUppsalaSweden Department of Clinical Intervention and Technology(CLINTEC) Karolinska InstitutetStockholmSweden

A recent publication in Nature by Lan et al.,presented GREM1(gremlin 1)as an important regulatory node of cellular plasticity in pancreatic ductal adenocarcinoma(PDAC).1 Knockout experiments in mice point to an important role of Grem1 in retaining an epithelial,differentiated phenotype of pancreatic cancer cells presumably by inhibiting BMP signalling.

关键词： al. cancer inhibiting

Isolation of Microcystins from the Cyanobacterium Planktothrix rubescens Strain No80

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Natural Products and Bioprospecting 2014年第1期4卷 37-45页

作者： Timo H.j.Niedermeyer Peter Schmieder rainer Kurmayer Cyano Biotech GmbH Magnusstr.1112489 BerlinGermany Interfaculty Institute for Microbiology and Infection Medicine Tubingen Eberhard Karls University TubingenAuf der Morgenstelle 2872076 TubingenGermany Leibniz Institut fur Molekulare Pharmakologie Robert-Rossle-Str.1013125 BerlinGermany Research Institute for Limnology University of InnsbruckMondseestrasse 95310 MondseeAustria

Three minor microcystins have been isolated from a Planktothrix rubescens *** structures have been elucidated by one-and two-dimensional NMR spectroscopy and high-resolution tandem mass spectrometry as the compounds[Asp^(3),(E)-Dhb^(7)]MC-LY(1),[Asp^(3),(E)-Dhb^(7)]MC-HtyW(2),and[Asp^(3),(E)-Dhb^(7)]MC-LW(3).The amino acids found at the variable positions 2 and 4 of the microcystin core structure are in accordance with the predicted amino acid substrate activation selectivities of the non-ribosomal peptide synthetases McyA and McyB described earlier for this *** structural microcystin variants produced by this strain were shown to inhibit protein phosphatase 1 in the nanomolar range.

关键词： Planktothrix Cyanobacteria Microcystins Protein phosphatase inhibition

Toward optical coherence tomography on a chip: in vivo three-dimensional human retinal imaging using photonic integrated circuit-based arrayed waveguide gratings

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Light(Science & Applications) 2021年第1期10卷 71-85页

作者： Elisabet A.Rank Ryan Sentosa Danielle j.Harper Matthias Salas Anna Gaugutz Dana Seyringer Stefan Nevlacsil Alej.ndro Maese-Novo Moritz Eggeling Paul Muellner rainer Hainberger Martin Sagmeister j.chen Kraft rainer ALeitgeb Wolfgang Drexler Center for Medical Physics and Biomedical Engineering Medical University of ViennaWaehringer Guertel 18-20/4 L1090 ViennaAustria Research Centre for Microtechnology Vorarlberg University of Applied SciencesHochschulstrasse 16850 DornbirnAustria AIT Austrian Institute of Technology GmbH Gieffinggasse 41210 ViennaAustria ams AG Tobelbader Strasse 308141 PremstaettenAustria

In this work,we present a significant step toward in vivo ophthalmic optical coherence tomography and angiography on a photonic integrated *** diffraction gratings used in spectral-domain optical coherence tomography can be replaced by photonic integrated circuits comprising an arrayed waveguide *** arrayed waveguide grating designs with 256 channels were tested,which enabled the first chip-based optical coherence tomography and angiography in vivo three-dimensional human retinal *** 1 supports a bandwidth of 22nm,with which a sensitivity of up to 91 dB(830μW) and an axial resolution of 10.7 pm was *** 2 supports a bandwidth of 48 nm,with which a sensitivity of 90 dB(480μW) and an axial resolution of 6.5μm was *** silicon nitride-based integrated optical waveguides were fabricated with a fully CMOS-compatible process,which allows their monolithic co-integration on top of an optoelectronic silicon *** a benchmark for chip-based optical coherence tomography,tomograms generated by a commercially available clinical spectral-domain optical coherence tomography system were compared to those acquired with on-chip *** similarities in the tomograms demonstrate the significant clinical potential for further integration of optical coherence tomography on a chip system.

关键词： waveguide arrayed optical

Determination of basal ileal endogenous losses and standardized ileal digestibility of amino acids in barley fed to growing pigs

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

j.urnal of Animal Science and Biotechnology 2017年第1期8卷 134-142页

作者： Hanna Katharina Spindler rainer Mosenthin Pia Rosenfelder Henry j.rgensen Knud Erik Bach Knudsen Meike Eklund University of Hohenheim Institute of Animal Science Aarhus University Department of Animal ScienceResearch Center Foulum

Background： Basal ileal endogenous amino acid（AA） losses（IAAend） and standardized ileal digestibility（SID） values of cereal grains, such as barley, are apparently underestimated when determined according to the nitrogen（N）-free method. Regression analysis between the dietary apparent ileal digestible content（c AID） and total crude protein（CP） and AA can be considered as alternative approach to obtain more accurate values for IAAendand SID of AA in cereal ***： Eight hulled barley genotypes were used, with barley being the only source of CP and AA in the assay diets. The diets contained 95 % as-fed of these eight barley genotypes each, ranging in CP content between 109.1 and 123.8 g/kg dry matter（DM）. Nine ileally T-cannulated barrows, average body weight（BW） 30 ± 2 kg, were allotted to a row-column design comprising eight periods with 6 d each and nine pigs. On d 5 and the night of d 6 of every period, ileal digesta were collected for a total of 12 h. The IAAend and the SID were determined by linear regression analysis between c AID and total dietary CP and ***： There exist linear relationships between cA ID and total CP and AA（P 〈 0.001）. The IAAend of CP, Lys, Met, Thr and Trp amounted to 35.34, 1.08, 0.25, 1.02 and 0.38 g/kg DM intake（DMI）, respectively, which are greater compared to average IAAend determined previously under N-free feeding conditions. The SID of CP, Lys, Met, Thr and Trp was 90,79, 85, 79 and 86 %, respectively, and was greater when compared to tabulated values. Moreover, these SID values were greater than those reported in literature, based on correction of apparent ileal digestibility（AID） of CP and AA for their IAAendvalues. Summarized, the results of the present regression analysis indicate greater IAAendin barley-based diets compared to those obtained by N-free ***： For low-protein feed ingredients like barley the regression method may be preferred over correction of AID values for their IAAenddetermined und

关键词： Amino acid Barley Basal ileal endogenous loss Growing pigs Regression analysis Standardized ileal digestibility

COmbination of Targeted temperature management and Thrombectomy after acute Ischemic Stroke(COTTIS):a pilot study

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Stroke & Vascular Neurology 2024年第3期9卷 258-267页

作者： j.rgen Bardutzky rainer Kollmar Forat Al-Rawi j.hann Lambeck Mohammad Fazel Christian Taschner Wolf-Dirk Niesen Department of Neurology and Neurophysiology Medical Center-University of FreiburgFaculty of MedicineUniversity of FreiburgFreiburgGermany Neurology and Neurointensive Care Darmstadt HospitalDarmstadtGermany BrainCool AB LundSweden Department of Neuroradiology Medical Center-University of FreiburgFaculty of MedicineUniversity of FreiburgFreiburgGermany

Background To evaluate the feasibility and safety of a fast initiation of cooling to a target temperature of 35℃by means of transnasal cooling in patients with anterior circulation large vessel occlusion(LVO)undergoing endovascular thrombectomy(EVT).Methods Patients with an LVO onset of<24hour who had an indication for EVT were included in the *** cooling(RhinoChill)was initiated immediately after the patient was intubated for EVT and continued until an oesophageal target temperature of 35℃was *** was maintained with surface cooling for 6-hour postrecanalisation,followed by active rewarming(+0.2℃/hour).The primary outcome was defined as the time required to reach 35℃,while secondary outcomes comprised clinical,radiological and safety *** Twenty-two patients(median age,77 years)were included in the study(14 received additional thrombolysis,4 additional stenting of the proximal internal carotid artery).The median time intervals were 309min for last-seen-normal-to-groin,58min for door-to-cooling-initiation,65min for door-to-groin and 123min for *** target temperature of 35℃was reached within 30min(range 13–78min),corresponding to a cooling rate of 2.6℃/*** recanalisation,86%of the patients had a body temperature of≤35℃.The median National Institutes of Health Stroke Scale at admission was 15 and improved to 2 by day 7,and 68%of patients had a good outcome(modified Rankin Scale 0–2)at 3 *** complications included asymptomatic bradycardia(32%),pneumonia(18%)and asymptomatic haemorrhagic transformation(18%).Conclusion The combined application of hypothermia and thrombectomy was found to be feasible in sedated and ventilated *** events were comparable to those previously described for EVT in the absence of *** effect of this procedure will next be evaluated in the randomised COmbination of Targeted temperature management and Thrombectomy after acute Ischemic Stro

关键词： maintained continued initiated

Effects of substrate surface treatments on hybrid manufacturing of AlSi7Mg using die casting and selective laser melting

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

j.urnal of Materials Science & Technology 2023年第25期156卷 142-156页

作者： Haiyang Fan Ann Witvrouw Friedrich Wolf-Monheim rainer Souschek Shoufeng Yang Department of Mechanical Engineering and Flanders Make KU LeuvenCelestijnenlaan 300Bbox 2420HeverleeB-3001 LeuvenBelgium Ford Research & Advanced Engineering Ford Research and Innovation Center AachenSüsterfeldstraße 20052072 AachenGermany Yantai Research Institute Harbin Engineering University264000 YantaiChina

To balance the manufacturing cost and customizability of automotive parts,a hybrid manufacturing process combining die-casting and selective laser melting(SLM)is proposed:starting with a conventional cast substrate,SLM is utilized to add additional geometric elements on top of *** this hybrid process,the first priority is to prepare a substrate surface suitable for the subsequent SLM addition of the top-on *** this study,the original cast surface of AlSi7Mg was processed by sandblasting,wire electro-discharge machining,and laser remelting,***,additional AlSi7Mg components were built on both the original cast and treated surfaces through *** hybrid builds,these surfaces and resultant interfaces were examined by optical and scanning electron *** indicate that the defect-free metallurgical j.int between the cast and additively added parts can be formed on all surfaces except for the one processed by electro-discharge *** observed epitaxial grain growth crossing the interface implies a strong connection between the cast and the SLMed *** these benefits,also mismatches in microstructure,residual stress level and element distribution between the two parts are *** a comprehensive assessment,laser remelting with no additional machining is recommended as the optimal surface treatment preceding SLM fabrication,because of its user-friendly operation,low cost,and high industrial feasibility.

关键词： Hybrid manufacturing Aluminium alloy Surface treatment Laser remelting Interface Automotive