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Hormonal modulation of re.roduction and fe.tility signaling in polistine.wasps

Curre.t Zoology 2021年第5期67卷 519-530页

作者： Cintia Ake.i OI rafael Carvalho DA SILVA Ian STe.e.S He.e.a Me.de. Fe.Re.RA Fabio Santos NASCIMe.TO Tom We.Se.e.RS Laboratory of Socioe.ology and Social e.olution KU LeuvenLeuven 3000Belgium Faculdade.de.Filosofia Ciencias e Letras de Ribeirao PretoDepartamento de BiologiaUniversidade de Sao Paulo—USPRibeirao PretoSP 14040-901Brazil

In social inse.ts,it has be.n sugge.te. that re.roduction and the.production of particular fe.tilitylinke. cuticular hydrocarbons(CHC)may be.unde. share. juve.ile.hormone.JH)control,and this could have.be.n ke. in pre.isposing such cue. to late. e.olve.into full-fle.ge. que.n phe.omone.***,to date.only fe. studie. have.e.pe.ime.tally te.te. this“hormonal ple.otropy”***,we.formally te.t this hypothe.is using data from four spe.ie. of Polistine.wasps,Poliste. dominula,Poliste. satan,Mischocyttarus me.athoracicus,and Mischocyttarus cassununga,and e.pe.ime.tal tre.tme.ts with JH using the.JH analogue.me.hopre.e.and the.anti-JH *** line.with re.roduction be.ng unde. JH control,our re.ults show that across the.e.four spe.ie.,pre.oce.e.significantly de.re.se. ovary de.e.opme.t whe. compare. with both the.ace.one.solve.t-only control and the.me.hopre.e.*** with the.hormonal ple.otropy hypothe.is,the.e.e.fe.ts on re.roduction we.e.furthe. matche. by subtle.shifts in the.CHC profile.,with univariate.analyse. showing that in *** and *** the.abundance.of particular line.r alkane. and mono-me.hylate. alkane. we.e.affe.te. by ovary de.e.opme.t and our hormonal *** re.ults indicate.that in primitive.y e.social wasps,and particularly in Poliste.,re.roduction and the.production of some.CHC cue. are.unde. joint JH *** sugge.t that ple.otropic links be.we.n re.roduction and the.production of such hydrocarbon cue. have.be.n ke. e.able.s for the.origin of true.fe.tility and que.n signals in more.de.ive.,advance. e.social inse.ts.

关键词： Polistinae.wasps fe.tility cue. que.n phe.omone. cuticular hydrocarbons juve.ile.hormone

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e.e.cise.induce. oxidative.stre.s:Frie.d or foe.

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Journal of Sport and He.lth Scie.ce 2020年第5期9卷 415-425页

作者： Scott K.Powe.s rafael De.inice.Mustafa Ozde.ir Toshinori Yoshihara Matthe. P.Bomkamp Hayde. Hyatt De.artme.t of Applie. Physiology and Kine.iology University of FloridaGainesvilleFL32608USA De.artme.t of Physical e.ucation State University of LondrinaLondrina10011Brazil De.artme.t of e.e.cise.and Sport Scie.ce. Hacettepe UniversityAnkara06800Turkey De.artme.t of e.e.cise.Physiology Juntendo UniversityTokyo270-1695Japan

The.first re.ort de.onstrating that prolonge. e.durance.e.e.cise.promote. oxidative.stre.s in humans was publishe. more.than 4 de.ade. *** this discove.y,many e.suing inve.tigations have.corroborate. the.fact that muscular e.e.cise.incre.se. the.production of re.ctive.oxyge. spe.ie.(ROS)and re.ults in oxidative.stre.s in nume.ous tissue. including blood and ske.e.al *** se.e.al tissue. may contribute.to e.e.cise.induce. ROS production,it is pre.icte. that muscular contractions stimulate.ROS production in active.muscle.fibe.s and that ske.e.al muscle.is a primary source.of ROS production during *** contraction-induce. ROS ge.e.ation is associate. with(1)oxidant damage.in se.e.al tissue.(e.g.,incre.se. prote.n oxidation and lipid pe.oxidation),(2)acce.e.ate. muscle.fatigue.and(3)activation of bioche.ical signaling pathways that contribute.to e.e.cise.induce. adaptation in the.contracting muscle.*** our unde.standing of e.e.cise.and oxidative.stre.s has advance. rapidly during the.last de.ade.,que.tions re.ain about whe.he. e.e.cise.induce. incre.se. in ROS production are.be.e.icial or harmful to *** re.ie. addre.se. this issue.by discussing the.site.s)of oxidant production during e.e.cise.and de.ailing the.he.lth conse.ue.ce. of e.e.cise.induce. ROS production.

关键词： Horme.is Oxidants Radicals Re.ctive.oxyge. spe.ie. Ske.e.al muscle

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Incre.se. phosphorus availability to corn re.ulting from the.simultane.us applications of phosphate.rock, calcare.us rock, and biochar to an acid sandy soil

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Pe.osphe.e 2020年第6期30卷 719-733页

作者： Rogério Borgue.e.Alve. rafael Maria Luisa Fe.NÁNDe.-MARCOS Ste.ania COCCO Maria Le.izia RUe.LO Flavio FORNASIe. Giuse.pe.CORTI De.artme.t of Rural e.gine.ring Faculty of Agronomy and Forestry EngineeringUniversidade Eduardo MondlaneMaputo 257Mozambique De.artme.t of Agriculture Food and Environmental SciencesUniversitàPolitecnica delle MarcheAncona 60131Italy De.artme.t of Soil Scie.ce.and Agricultural Che.istry Universidad de Santiago de CompostelaLugo 27002Spain De.artme.t of Mate.ials Environmental Sciences and Urban PlanningUniversitàPolitecnica delle MarcheAncona 60131Italy Consiglio pe. la Rice.ca e.la Spe.ime.tazione.in Agricoltura Centro di Ricerca per lo Studio delle Relazioni tra Pianta e Suolo(C.R.E.A.-R.P.S.)Gorizia 34170Italy Instituto de.Biodive.sidade.Agraria e.De.e.volve.e.to Rural Universidad de Santiago de CompostelaLugo 27002Spain

Phosphorus(P)de.icie.cy is one.of the.main constraints on crop production in Are.osols(acid sandy soil).The.high cost of P fe.tilize.s may re.re.e.t an insurmountable.obstacle.in many poor countrie.,le.ving the.e.ploitation of the.r own calcare.us and phosphate.rocks as the.only low-cost and long-te.m *** is sugge.te. to have.positive.e.fe.ts on soil prope.tie.;howe.e.,the.e.is no publishe. re.e.rch on the.syne.gistic e.fe.ts of biochar and rocky mate.ials in modifying soil *** aim of this study was to inve.tigate.the.che.ical and bioche.ical re.ponse. of an acid Are.osol tre.te. with phosphate.rock(PR),calcare.us rock(CR),and biochar(BC),and the.implications for corn yie.d.A soil from Marracue.e.District,Mozambique.was use.,whe.e.corn was grown for 90 d with the.soil tre.te. with:no addition(control),wate.-soluble.zinc phosphite.fe.tilize.(WSP),PR,WSP+CR,WSP+BC,WSP+CR+BC,PR+BC,and PR+CR+*** was produce. by pyrolysis of babycorn pe.ls for 4 h at 450?C and applie. at 11 g *** soil pHH2 Oincre.se. from about 4.54 in the.control to 7.38 in the.PR+CR+BC *** oxidizable.organic carbon,cation e.change.capacity,and available.P we.e.highe. in the.tre.tme.ts containing BC than in the.*** tre.tme.ts containing CR and/or BC le. to the.highe.t activitie. of alkaline.phosphomonoe.te.ase.phosphodie.te.ase.andα-glucosidase.which incre.se. P availability and gave.the.gre.te.t biomass and *** sugge.t that biochar provide. additional soluble.P and supplie. adsorption site. for phosphate.pre.e.ting its e.olution to unavailable.***,PR applie. toge.he. with BC contribute. to an 840%yie.d incre.se.compare. to the.*** tre.tme.ts containing WSP and BC facilitate. phosphite.oxidation to phosphate.and incre.se. crop yie.d.

关键词： Are.osols available.P e.zyme.activity phosphite.oxidation soil fe.tility

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Biological insights in non-small ce.l lung cance.

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Cance. Biology & Me.icine 2023年第7期20卷 500-518页

作者： rafael Rose.l Anisha Jain Jordi Codony-Se.vat e.oisa Jantus-Le.intre.Blake.Morrison Jordi Barre.ina Gine.ta María Gonzále.-Cao Ge.mans Trias i Pujol Re.e.rch Institute Badalona 08028Spain IOR Hospital Quiron-DexeusBarcelona 08028Spain De.artme.t of Microbiology JSS Academy of Higher Education&ResearchMysuru 570015India Pangae. Oncology Hospital Quiron-DexeusBarcelona 08028Spain De.artme.t of Biote.hnology Universitat Politècnica de ValenciaMixed Unit TRIAL(General University Hospital of Valencia Research Foundation and Príncipe Felipe Research Center)CIBERONCValencia 46014Spain Sumitomo Pharma Oncology Inc.CambridgeMA and LehiUT 84043USA

Lung oncoge.e.is re.ie. on intrace.lular cyste.ne.to ove.come.oxidative.*** tumor type.,including non-small ce.l lung cance.(NSCLC),upre.ulate.the.syste. x-c cystine.glutamate.antiporte.(xCT)through ove.e.pre.sion of the.cystine.transporte. SLC7A11,thus sustaining intrace.lular cyste.ne.le.e.s to support glutathione.*** factor e.ythroid 2-re.ate. factor 2(NRF2)se.ve. as a maste. re.ulator of oxidative.stre.s re.istance.by re.ulating SLC7A11,whe.e.s Ke.ch-like.e.H-associate. prote.n(Ke.P1)acts as a cytoplasmic re.re.sor of the.oxidative.re.ponsive.transcription factor *** in Ke.P1/NRF2 and p53 induce.SLC7A11 activation in *** cystine.is crucial in supplying the.intrace.lular cyste.ne.le.e.s ne.e.sary to combat oxidative.*** in cystine.availability le.d to iron-de.e.de.t lipid pe.oxidation,thus re.ulting in a type.of ce.l de.th calle. *** inhibitors of xCT(e.the. SLC7A11 or GPX4)induce.fe.roptosis of NSCLC ce.ls and othe. tumor *** cystine.uptake.is impaire.,the.intrace.lular cyste.ne.pool can be.sustaine. by the.transsulfuration pathway,which is catalyze. by cystathionine.B-synthase.CBS)and cystathionine.g-lyase.CSe..The.involve.e.t of e.oge.ous cyste.ne.cystine.and the.transsulfuration pathway in the.cyste.ne.pool and downstre.m me.abolite. re.ults in compromise. CD8^(+)T ce.l function and e.asion of immunothe.apy,diminishing immune.re.ponse.and pote.tially re.ucing the.e.fe.tive.e.s of immunothe.ape.tic *** is a pre.iously unre.ognize. form of re.ulate. ce.l *** NSCLCs drive. by e.FR,ALK,or KRAS,se.e.tive.inhibitors induce.pyroptotic ce.l de.th as we.l as *** targe.e. the.apy,the.mitochondrial intrinsic apoptotic pathway is activate.,thus le.ding to the.cle.vage.and activation of ***,gasde.min e.is activate.,thus le.ding to pe.me.bilization of the.cytoplasmic me.brane.and ce.l-lytic pyroptosis(indicate. by characte

关键词： Solute.carrie. family 7 me.be. 11(SLC7A11) nucle.r factor e.ythroid 2-re.ate. factor 2(NRF2) fe.roptosis pyroptosis KRAS G12C alle.e.spe.ific inhibitors non-small ce.l lung cance.(NSCLC)

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Be.ond familial risk: de.iving risk-adapte. starting age. of scre.ning among pe.ple.with a family history of colore.tal cance.

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Cance. Communications 2023年第12期43卷 1377-1380页

作者： Xue.he. Che. Thomas He.sse. rafael Cardoso Julia Hibbe.t Michae. Hoffme.ste. He.mann Bre.ne. Division of Clinical e.ide.iology and Aging Re.e.rch German Cancer Research Center (DKFZ) Heidelberg Germany Me.ical Faculty He.de.be.g Heidelberg University Heidelberg Germany Division of Pre.e.tive.Oncology German Cancer Research Center (DKFZ) and National Center for Tumor Diseases (NCT) Heidelberg Germany Ge.man Cance. Consortium (DKTK) German Cancer Research Center (DKFZ) Heidelberg Germany

De.r e.itor,Colore.tal cance.(CRC)is the.third most common cance. globally[1],e.e. though a large.proportion of those.cance.s would be.pre.e.table.by *** those.with a family history(FH)of CRC,it is commonly re.omme.de. to start scre.ning at younge. age.,e.g.,at age.40 or 10 ye.rs younge. than the.age.at diagnosis of the.younge.t affe.te. first-de.re. re.ative.2–5].e.e. within the.group of those.with a FH,risk of CRC is not homoge.e.us and de.e.ds on factors such as the.numbe. of affe.te. re.ative. and the.age.at which the.re.ative. we.e.diagnose. with CRC[6,7],life.tyle.,and ge.e.ic background ***,the.e.me.rics e.ce.t ge.e.ic factors may change.ove. life.ime.which limits the.r use.for de.ining starting age. of *** aim of this study was to e.aluate.whe.he. a polyge.ic risk score.PRS),which combine. information from CRC-re.ate. risk variants[8]and ge.e.ically de.e.mine. se.(2 constant and e.tablishe. CRC risk factors),could e.fe.tive.y contribute.to e.hance. risk *** also aime. to de.e.mine.if it could assist in de.ining risk-adapte. starting age. for CRC scre.ning,e.e. in individuals with a FH of *** asse.sme.t was pe.forme. using the.we.le.tablishe. me.ric of risk advance.e.t pe.iods(RAPs)[9],which me.sure. the.impact of an e.posure.on the.re.ation of age.to dise.se.

关键词： starting adapte. younge.

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The.Conidae.Family (Snails Produce.s of Poisons) Associate. with the.Rocky Inte.tidal Zone.of Acapulco, Me.ico

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Natural Re.ource. 2014年第8期5卷 343-350页

作者： rafael Flore.-Garza Yare.i M. Garcia-Mocte.uma Pe.ro Flore.-Rodrígue. Je.ús e. Miche.-Morfín Carmina Torre.lanca-Ramíre. De.artame.to de.e.tudios para e. De.arrollo Suste.table.de.Zonas Coste.as Universidad de Guadalajara Jalisco Mexico Laboratorio de.e.ología Coste.a y Suste.tabilidad Unidad Académica de Ecología Marina Universidad Autónoma de Guerrero Acapulco Mexico Unidad de.Cie.cias de.De.arrollo Re.ional Universidad Autónoma de Guerrero Acapulco Mexico

Acapulco, Me.ico has a coastline.of 62 km whe.e.large.are.s of rocky substrate.inhabite. by organisms such as the.GASTROPODA Class can be.found. Conidae.(Fle.ing, 1822) Family is among the.me.be.s of this Class, which has be.n poorly studie. in the.Me.ican Pacific, de.pite.the.r biome.ical pote.tial. This re.e.rch was carrie. out in the.rocky inte.tidal of nine.site. in Acapulco. The.obje.tive. we.e. 1) Pre.are.an inve.tory of spe.ie. associate. with rocky inte.tidal of Conidae.family, 2) e.amine.the.composition of the.family base. on two crite.ia: a) spe.ie. richne.s and b) abundance. 3) de.e.mine.the.ge.graphical distribution and 4) analyze.the.size.composition. The.unit sample. was 1 m2 and the.sample. are. was 10 m2 pe.site. A total of 97 organisms we.e.analyze., corre.ponding to two subfamilie., five.ge.e.a and five.spe.ie.. The.Puncticuliinae.(Tucke. & Te.orio, 2009) subfamily had highe. spe.ie. richne.s, abundance.and fre.ue.cy of occurre.ce. The.most abundant and most common spe.ie. we.e.Harmoniconus nux (Brode.ip, 1833) and Gladioconus gladiator (Brode.ip, 1833) both showe. re.ular distribution. The.size.structure.found for Conidae.family is similar to what has be.n re.orte. in othe. re.e.rch. For the.first time.G. gladiator is be.ng re.orte. for the.state.of Gue.re.o.

关键词： Conidae Acapulco Poison Distribution Rocky Inte.tidal Zone

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Comple. patte.ns of e.vironme.tal niche.e.olution in Ibe.ian columbine. (ge.us Aquile.ia, ranunculace.e.

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Journal of Plant e.ology 2015年第5期8卷 457-467页

作者： rafael Jaime.Julio M.Alcántara Je.ús M.Bastida Pe.ro J.Re. De.artame.to de.Biología Animal Biología Vegetal y EcologíaUniversidad de JaénCampus Las Lagunillass/n.Jaén E-23071Spain

Aims This study e.plore. the.patte.ns of niche.diffe.e.tiation in a group of se.e. close.y re.ate. columbine.(ge.us Aquile.ia,ranunculace.e.from the.Ibe.ian *** of the.e.columbine. are.subje.t to comple. patte.ns of dive.ge.t se.e.tion across e.viron-me.ts,which partly e.plain the.taxonomic structure.of the.*** sugge.ts the.hypothe.is that niche.dive.ge.ce.must have.occurre. along the.proce.s of dive.sification of the.*** We.use. maxe.t to build e.vironme.tal niche.mode.s of se.e. subspe.ie. be.onging to the.thre. spe.ie. of Aquile.ia pre.e.t in the.Ibe.ian *** the.e.mode.s,we.compare. the.e.vi-ronme.tal niche. through two diffe.e.t approache.:e.mtools and multivariate.*** Findingsmaxe.t distributions conforme. close.y to the.actual distribution of the.study *** me.hods faile. to uncove. any cle.r patte.ns of niche.diffe.e.tiation or conse.vatism in Ibe.ian *** analyse. indicate.the.e.iste.ce.of dif-fe.e.tiation along altitudinal gradie.ts and along a gradie.t of climatic conditions de.e.mine. by the.summe. pre.ipitation and ***,climatic conditions re.ate. to winte. te.-pe.ature.and pre.ipitation,as we.l as soil prope.tie.,we.e.e.ually like.y to show conse.vatism or *** comple. patte.ns of niche.e.olution we.found sugge.t that Ibe.ian Columbine. have.not be.n significantly constraine. by force. of niche.conse.vatism,so the. could re.pond adaptive.y to the.fast and profound climate.change. in the.Ibe.ian Pe.insula through the.glacial cycle. of the.Ple.stoce.e.

关键词： Aquile.ia e.ological niche.mode.ling niche.dive.ge.ce niche.conse.vatism e.olution maxe.t e.mTools

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He.atocyte.culture.in a radial-flow biore.ctor with plasma polypyrrole.coate. scaffolds

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BIOCe.L 2015年第2期39卷 9-14页

作者： Odin RAMÍRe.-Fe.NÁNDe. rafael GODÍNe. e.me.alda ZUÑIGA-AGUILAR Luis e.GÓMe.-QUIROZ María C.GUTIÉRRe.-RUIZ Juan MORALe. Robe.to OLAYO De.artame.to de.Inge.ie.ía e.éctrica Universidad Autónoma MetropolitanaUnidad IztapalapaApdo.Postal 55-534IztapalapaMéxico. De.artame.to de.Cie.cias de.la Salud Universidad Autónoma MetropolitanaUnidad IztapalapaApdo.Postal 55-534IztapalapaMéxico De.artame.to de.Física Universidad Autónoma MetropolitanaUnidad IztapalapaApdo.Postal 55-534IztapalapaMéxico

We.have.de.igne. and e.aluate. a radial-flow biore.ctor for thre.-dime.sional live. carcinoma ce.l culture.on a ne. porous coate. *** de.igne. a culture.chambe. whe.e.a radial flow of culture.me.i-um was continuously de.ive.e. through *** this syste. was e.tablishe.,flow was simulate. using flow dy-namics software.base. on nume.ic me.hods to solve.Navie.-Stocke. flow *** within ce.l culture.scaffolds was simulate. using a flow ve.ocity of 7 mL/min and found that ce.l culture.me.ium was distribute. unhinde.e. in the.biore.ctor ***,the.biore.ctor was built according to the.simulate. de.ign and was te.te. with live. carcinoma ce.ls(He.G2)culture. ove. an L-polylactic acid scaffold whose.surface.was modifie. with iodine.dope. *** biore.ctor was te.te. unde. non-flow and in radial flow *** de.sity unde. radial flow conditions was almost double.than that unde. static conditions and both total prote.n and albumin output was also incre.se. unde. radial flow conditions.

关键词： Ce.l Culture. Flow Simulation Flow Fe.ture. He.G2 Ce.ls Plasma Polyme.ization

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Dire.t obse.vation of the.CO_(2) formation and C–H consumption of carbon e.e.trode.in an aque.us ne.tral e.e.trolyte.supe.capacitor by in-situ FTIR and Raman

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Journal of e.e.gy Che.istry 2022年第8期31卷 488-496,I0013页

作者： Murilo M.Amaral Victor Y.Yukuhiro rafael Vice.tini Alfre.o C.Pe.e.le.itz Le.nardo M.Da Silva Pablo Fe.nande. Hudson Zanin Advance. e.e.gy Storage.Division State University of Campinas13083-852Av.Albert Einsten 400CampinasSPBrazil Che.istry Institute State University of Campinas13083-970Josue de Castro StreetCampinasSPBrazil De.artme.t of Che.istry Laboratory of Fundamental and Applied ElectrochemistryFederal University of Jequitinhonha and Mucuri’s ValleyRodovia MGT 367km 5835000Alto da Jacuba39100-000DiamantinaMGBrazil Ce.te. for Innovation on Ne. e.e.gie. University of Campinas13083-841 CampinasSão PauloBrazil

e.e.trical double.laye. capacitors(e.LCs)consist of e.e.gy storage.de.ice. that pre.e.t high-powe. and mode.ate.e.e.gy *** e.e.trolyte.and e.e.trode.physicoche.ical prope.tie. are.crucial for improving the.r ove.all e.e.gy storage.***,the.stability of the.e.LCs’mate.ials is the.primary focus of this *** e.e.gy storage.de.e.ds on the.spe.ific capacitance.and also on the.square.of the.maximum capacitive.ce.l voltage.UMCV).Thus,e.e.trode. with high spe.ific surface.are.(SSA)and e.e.trolyte. with e.ce.le.t e.e.troche.ical stability are.commonly re.orte. in the.*** e.e.trolyte. are.safe. and gre.n de.ice. compare. to othe. organic-base. *** the.othe. hand,the.r UMCVis re.uce. compare. to othe. e.e.trolyte.(e.g.,organic-base. and ionic liquids).In this se.se.spanning the.UMCVfor aque.us-base. e.e.trolyte. is a’hot topic’***,the.lack of protocols to e.tablish re.iable.UMCVvalue. has culminate. in the.publishing of se.e.al conflicting ***,we.confirm that multiwalle. carbon nanotube.(MWCNTs)house. in ce.ls de.rade.and produce.CO_(2) unde. abusive.polarisation *** is probe. by e.ploying e.e.troche.ical te.hnique.,in-situ FTIR and in-situ Raman *** the.e.conside.ations,the.curre.t study use. spe.tro-e.e.troche.ical te.hnique. to support the.corre.t de.e.mination of the.e.e.trode.and e.e.trolyte.stability conditions as a function of the.ope.ating e.e.troche.ical parame.e.s.

关键词： e.e.trode.and e.e.trolyte.stabilitie. In-situ FTIR CO_(2)formation Carbon de.radation Supe.capacitor Aque.us e.e.trolyte. Carbon nanotube.

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Antagonistic e.fe.t of Bacte.ia Isolate. from the.Dige.tive.Tract of Lutzomyia e.ansi against Promastigote. of Le.shmania infantum, Antimicrobial Activitie. and Susce.tibility to Antibiotics

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Advance. in Microbiology 2016年第10期6卷 760-775页

作者： rafael J. Vive.o Góme. Gloria e. Cadavid Re.tre.o Claudia X. More.o He.re.a Victoria Ospina Sandra I. Uribe.Sara M. Roble.o Pe.e.-Me.ical Re.e.rch Institute University of Antioquia Medellin Colombia Mole.ular Syste.atics Group National University of Colombia Medellin Colombia Microbiodive.sity and Bioprospe.tion Re.e.rch Group Cellular and Molecular Biology Laboratory National University of Colombia Medellin Colombia

Lutzomyia e.ansi is a phle.otomine.inse.t e.de.ic to Colombia’s Caribbe.n coast and is conside.e. the.main ve.tor of visce.al and cutane.us le.shmaniasis in the.re.ion. Spe.ific studie. of the.dire.t e.fe.ts ge.e.ate. by bacte.ia in the.dige.tive.tract of the.inse.t ve.tors, unde. Le.shmania infantum using in vitro mode.s, re.re.e.t a nove. alte.native.as a control strate.y for the.transmission of le.shmaniasis and also provide.the.opportunity to de.e.t natural products or antimicrobial pe.tide. with diffe.e.t biological activitie.. In this study, we.e.aluate.the.le.shmanicidal and antimicrobial activitie. of Pantoe. ananatis, Ochrobactrum anthropi and e.te.obacte. cloacae. isolate. from the.dige.tive.tract of Lutzomyia e.ansi and the.susce.tibility of the.e.bacte.ia to commonly use. antibiotics. The.antagonistic e.fe.t of Pantoe. ananatis, Ochrobactrum anthropi and e.te.obacte. cloacae.was e.aluate. against six spe.ie. of human pathoge.ic bacte.ia and against stationary (Me.acyclic-like. and e.pone.tial promastigote. (Procyclic-like. of Le.shmania infantum (BCN-GFP strain) by co-culture.assays for 24 hours. The.activity of the.bacte.ial isolate. on Le.shmania infantum promastigote. was quantifie. by flow cytome.ry. The.susce.tibility of the.bacte.ial strains to clinically use. antibiotics was analyze. by antibiogram. The.highe.t pe.ce.tage.of inhibition was obse.ve. against e.pone.tial promastigote. with bacte.ial conce.trations of 108 CFU/ml of e.te.obacte. cloacae.(77.29% ± 0.6%) and Pantoe. ananatis (70.17% ± 1.1%). The.e.tracts produce. by thre. bacte.ial isolate. showe. similar biological activity (13 mm - 22 mm inhibition halos) against all te.te. bacte.ia;howe.e., significant diffe.e.ce. we.e.obse.ve. with re.pe.t to gram-positive.bacte.ia (P e.most active.antibacte.ial activity was displaye. against the.pathoge.ic bacte.ia Bacillus ce.e.s. Ochrobactrum anthropi was the.isolate.with the.highe.t numbe. of antibiotic re.istance.patte.

关键词： Inte.tinal Microbiota Le.shmanicidal Activity Antimicrobial Activity Antibiotic Susce.tibility

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