Hepatocyte culture in a radial-flow bioreactor with plasma polypyrrole coated scaffolds
作者机构：Departamento de Ingeniería EléctricaUniversidad Autónoma MetropolitanaUnidad IztapalapaApdo.Postal 55-534IztapalapaMéxico. Departamento de Ciencias de la SaludUniversidad Autónoma MetropolitanaUnidad IztapalapaApdo.Postal 55-534IztapalapaMéxico Departamento de FísicaUniversidad Autónoma MetropolitanaUnidad IztapalapaApdo.Postal 55-534IztapalapaMéxico
出 版 物：《BIOCELL》 (生物细胞（英文）)
年 卷 期：2015年第39卷第2期
页 面：9-14页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Universidad Autónoma Metropolitana(UAM),Consejo Nacional de Ciencia y Tecnología(CONACYT)(project 155239) Instituto de Ciencia y Tecnología del Distrito Federal(ICyT-DF)(PIUTE 10-63276/2010)y(PICSA 11-14/2011)for funding
主 题：Cell Cultures Flow Simulation Flow Features HepG2 Cells Plasma Polymerization
摘 要：We have designed and evaluated a radial-flow bioreactor for three-dimensional liver carcinoma cell culture on a new porous coated *** designed a culture chamber where a radial flow of culture medi-um was continuously delivered through *** this system was established,flow was simulated using flow dy-namics software based on numeric methods to solve Navier-Stockes flow *** within cell culture scaffolds was simulated using a flow velocity of 7 mL/min and found that cell culture medium was distributed unhindered in the bioreactor ***,the bioreactor was built according to the simulated design and was tested with liver carcinoma cells(HepG2)cultured over an L-polylactic acid scaffold whose surface was modified with iodine-doped *** bioreactor was tested under non-flow and in radial flow *** density under radial flow conditions was almost double than that under static conditions and both total protein and albumin output was also increased under radial flow conditions.
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