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Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase Ⅱ in spinal cord injury rats

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Neural Regeneration Research 2017年第2期12卷 276-282页

作者： You-jiang Min Li-li-qiang ding Li-hong Cheng Wei-ping Xiao Xing-wei He Hui Zhang Zhi-yun Min Jia Pei Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine Nanchang Jiangxi Province China Shanghai University of Traditional Chinese Medicine Shanghai China

Electroacupuncture is beneficial for the recovery of spinal cord injury, but the underlying mechanism is unclear. The Rho/Rho-associated kinase（ROCK） signaling pathway regulates the actin cytoskeleton by controlling the adhesive and migratory behaviors of cells that could inhibit neurite regrowth after neural injury and consequently hinder the recovery from spinal cord injury. Therefore, we hypothesized electroacupuncture could affect the Rho/ROCK signaling pathway to promote the recovery of spinal cord injury. In our experiments, the spinal cord injury in adult Sprague-Dawley rats was caused by an impact device. Those rats were subjected to electroacupuncture at Yaoyangguan（GV3）, Dazhui（GV14）, Zusanli（ST36） and Ciliao（BL32） and/or monosialoganglioside treatment. Behavioral scores revealed that the hindlimb motor functions improved with those treatments. Real-time quantitative polymerase chain reaction, fluorescence in situ hybridization and western blot assay showed that electroacupuncture suppressed the m RNA and protein expression of Rho-A and Rho-associated kinase Ⅱ（ROCKⅡ） of injured spinal cord. Although monosialoganglioside promoted the recovery of hindlimb motor function, monosialoganglioside did not affect the expression of Rho-A and ROCKⅡ. However, electroacupuncture combined with monosialoganglioside did not further improve the motor function or suppress the expression of Rho-A and ROCKⅡ. Our data suggested that the electroacupuncture could specifically inhibit the activation of the Rho/ROCK signaling pathway thus partially contributing to the repair of injured spinal cord. Monosialoganglioside could promote the motor function but did not suppress expression of Rho A and ROCKⅡ. There was no synergistic effect of electroacupuncture combined with monosialoganglioside.

关键词： nerve regeneration spinal cord injury electroacupuncture Rho/Rho-associated kinase signaling pathway monosialoganglioside motor function cytoskeleton real-time quantitative polymerase chain reaction western blot assay hybridization in situ neural regeneration

Renal Protective Activity of Hsian-tsao Extracts in Diabetic Rats

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Biomedical and Environmental Sciences 2008年第3期21卷 222-227页

作者： MIN YANG ZHENG-PING XU CAI-JU XU JIA MENG GANG-qiang ding XIAO-MING ZHANG YAN WENG Zhejiang University School of Medicine Hangzhou 310058 Zhejiang China Zhejiang Provincial Center for Disease Control and Prevention Hangzhou 310009 Zhejiang China

Objective To investigate the renal protective activity of Hsian-tsao Mesona procumbens Hemsl. water extracts in diabetic rats. Methods Thirty Sprague-dawley female rats were randomly divided into three groups （n=10 each）, ＂control group＂ with intraperitoneal saline injection, ＂diabetic group＂ with 60 mg of intraperitoneal streptozotocin injection per kg of body weight and ＂Hsian-tsao group＂ with intragastric administration of Hsian-tsao extraction everyday for 4 weeks after intraperitoneal streptozotocin injection. The body weight and blood sugar were measured before and after model induction in the three groups. Thrombospondin-1 （TSP-1） expressions in the kidney were monitored by immunohistochemistry. Kidney ultrastructural changes were also analyzed by using transmission electron microscopy. Results Before diabetic model induction, there were no significant differences among the three groups in body weight and blood sugar. Four weeks after the induction of diabetes, the differences became statistically significant. Electron microscopy also revealed disruption of the foot processes of the podocytes and other damages in diabetic group. These damages were significantly less severe in Hsian-tsao group when compared with the diabetic group. TSP-1 expressions in the kidney were significantly increased in both the diabetic group and Hsian-tsao group, but it was relatively lower in Hsian-tsao group than in diabetic group. Conclusion Our results showed that Hsian-tsao treatment in the diabetic rats effectively prevented the pathological alterations in the kidney and decreased the TSP- 1 expression. It was suggested that Hsian-tsao had protective effect on the kidneys of the diabetic rats.

关键词： Rat Mesona procumbens Hemsl. Diabetic nephropathy Thrombospondin-1

基于孤立森林与KDE-LOF的冷水机组故障检测

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计算机应用与软件 2023年第1期40卷 84-89页

作者：熊坤丁强祝红梅杭州电子科技大学自动化学院浙江杭州310018

为了提升冷水机组微小故障的检测率,提出一种核密度估计的局部异常因子算法(KDE-LOF)结合孤立森林(iForest)的冷水机组故障检测策略。该策略通过使用孤立森林对实验数据异常值进行剔除,计算正常数据的LOF值作为统计量,并使用KDE确定控... 详细信息

为了提升冷水机组微小故障的检测率,提出一种核密度估计的局部异常因子算法(KDE-LOF)结合孤立森林(iForest)的冷水机组故障检测策略。该策略通过使用孤立森林对实验数据异常值进行剔除,计算正常数据的LOF值作为统计量,并使用KDE确定控制限来完成模型的训练。通过监测数据LOF值是否超过设定的控制限进而判断是否出现故障。采用ASHRAE RP-1043数据集进行验证,并分析了该方法与主元分析和单类支持向量机的方法的优劣,结果表明该方法检测效果要优于其他两种模型,该方法在微小故障下检测率超过80%,性能最佳。

关键词：故障检测冷水机组局部异常因子核密度估计孤立森林

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五相直线电机混合空间矢量脉宽调制控制策略

机床与液压 2022年第20期50卷 70-77页

作者：丁强石滕瑞鲍旭聪南京工业职业技术大学电气工程学院江苏南京210023 江苏省风力发电工程技术中心江苏南京210023 南京航空航天大学自动化学院江苏南京210016

直线电机能实现直接直线传动,不存在中间转换传动装置,转换效率高,且相较于传统旋转电机不存在离心力的约束,在工业中应用广泛。为进一步提高系统推力密度,以五相直线电机为研究对象。搭建五相直线电机离散域解耦控制数学模型,并针对传... 详细信息

直线电机能实现直接直线传动,不存在中间转换传动装置,转换效率高,且相较于传统旋转电机不存在离心力的约束,在工业中应用广泛。为进一步提高系统推力密度,以五相直线电机为研究对象。搭建五相直线电机离散域解耦控制数学模型,并针对传统五相直线电机单空间矢量脉宽调制控制策略运行性能差、系统谐波含量高的问题,提出一种五相直线电机双混合空间矢量脉宽调制控制策略。通过引入额外电压矢量,合理分配电压矢量作用时间,能有效抑制谐波,提升电机运行性能。仿真与实验均充分验证了所提五相直线电机双混合空间矢量脉宽调制控制策略的有效性及优越性。

关键词：五相直线电机单空间矢量混合空间矢量空间矢量脉宽调制谐波含量

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Research in Astronomy and Astrophysics 2020年第1期20卷 1-5页

作者：苏定强白华崔向群 School of Astronomy and Space Science&Key Laboratory of the Ministry of Education Nanjing UniversityNanjing 210023China National Astronomical Observatories/Nanjing Institute of Astronomical Optics and Technology(NIAOT) Chinese Academy of ScienceNanjing 210042China

FAST,the largest single-dish radio telescope in the world,has a 500-meter diameter main reflector and a 300-meter diameter illuminated *** has a main reflector that can vary its shape,which continuously changes the shape of the illuminated area in reflector into a *** this article,we propose a quasiCassegrain system for *** detailed design results are *** a quasi-Cassegrain system only needs to add a 14.6-meter diameter secondary reflector,which is close to the size of the feed *** distance from the secondary reflector to the focus is only 5.08 m,and it has excellent image *** this quasi-Cassegrain system,the shape of the illuminated area in the main reflector continuously changes into an optimized *** this quasi-Cassegrain system from frequency 0.5 GHz to 8 GHz,the multi-beam system can include 7 to 217 *** this system is used in combination with Phased Array Feed(PAF)technology,more multi-beam feeds or a higher working frequency can be used.

关键词： telescope techniques:miscellaneous methods:miscellaneous instrumentation:miscellaneous surveys

A novel mouse model of human breast cancer stem-like cells with high CD44^＋CD24^–/lower phenotype metastasis to human bone

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Chinese Medical Journal 2008年第20期121卷 1980-1986页

作者： LING Li-jun WANG Shui LIU Xiao-an SHEN En-chao ding qiang LU Chao XU Jian CAO Qin-hong ZHU Hai-qing WANG Feng Department of General Surgery First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China Department of Pathology Nanjing Brain Hospital Affiliated to Nanjing Medical University Nanjing Jiangsu 210029 China Department of Nuclear Medicine First Nanjing Hospital Affiliated to Nanjing Medical University Nanjing Jiangsu 210006 China

Background A satisfactory animal model of breast cancer metastasizing to bone is unavailable. In this study, we used human breast cancer stem-like cells and human bone to build a novel “human-source” model of human breast cancer skeletal metastasis. Methods Human breast cancer stem-like cells, the CD44^＋/CD24^-/lower subpopulation, was separated and cultured. Before injection with the stem-like cells, mice were implanted with human bone in the right or left dorsal flanks. Animals in Groups A, B, and C were injected with 1×10^5, 1×10^6 human breast cancer stem-like cells, and 1×10^6 parental MDA-MB-231 cells, respectively. A positive control group （D） without implantation of human bone was also injected with 1×10^6 MDA-MB-231 cells. Immunohistochemistry was performed for determination of CD34, CD105, smooth muscle antibody, CD44, CD24, cytokine, CXC chemokine receptor-4 （CXCR4）, and osteopontin （OPN）. mRNA levels of CD44, CD24, CXCR4, and OPN in bone metastasis tissues were analyzed by real-time quantitative polymerase chain reaction （PCR）.Results Our results demonstrated that cells in implanted human bones of group B, which received 1×10^6 cancer stem-like cells, stained strongly positive for CD44, CXCR4, and OPN, whereas those of other groups showed no or minimum staining. Moreover, group B had the highest incidence of human bone metastasis （77.8%, P=0.0230） and no accompaniment of other tissue metastasis. The real-time PCR showed an increase of CD44, CXCR4, and OPN mRNA in metastatic bone tissues in group B compared with those of groups C and D, however the expression of CD24 mRNA in group B were the lowest. Conclusions In the novel “human source” model of breast cancer, breast cancer stem-like cells demonstrated a higher human bone-seeking ability. Its mechanism might be related to the higher expressions of CD44, CXCR4, and OPN, and the lower expression of CD24 in breast cancer stem-like cells.

关键词： breast cancer cancer stem-like cells human source bone metastasis animal model

一类连续时间Markov跳跃系统鲁棒H_∞故障估计

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控制与决策 2009年第10期24卷 1555-1558页

作者：丁强钟麦英山东大学控制科学与工程学院济南250061 北京航空航天大学惯性技术与导航仪器系北京100191

研究了受L2范数有界未知输入影响的一类线性连续时间Markov跳跃系统鲁棒H∞故障估计问题.应用自适应观测器作为故障估计器,将鲁棒H∞故障估计问题归结为随机H∞滤波问题.推导并证明了问题可解的充分条件,并通过求解线性矩阵不等式得到了... 详细信息

研究了受L2范数有界未知输入影响的一类线性连续时间Markov跳跃系统鲁棒H∞故障估计问题.应用自适应观测器作为故障估计器,将鲁棒H∞故障估计问题归结为随机H∞滤波问题.推导并证明了问题可解的充分条件,并通过求解线性矩阵不等式得到了H∞故障估计器参数矩阵的解.最后,数字算例验证了所提方法的有效性.

关键词： Markov跳跃系统故障估计自适应观测器线性矩阵不等式

基于评价网络近似误差的自适应动态规划优化控制

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控制与决策 2015年第3期30卷 495-499页

作者：林小峰丁强广西大学电气工程学院南宁530004

为了求解有限时域最优控制问题,自适应动态规划(ADP)算法要求受控系统能一步控制到零.针对不能一步控制到零的非线性系统,提出一种改进的ADP算法,其初始代价函数由任意的有限时间容许序列构造.推导了算法的迭代过程并证明了算法的收敛性... 详细信息

为了求解有限时域最优控制问题,自适应动态规划(ADP)算法要求受控系统能一步控制到零.针对不能一步控制到零的非线性系统,提出一种改进的ADP算法,其初始代价函数由任意的有限时间容许序列构造.推导了算法的迭代过程并证明了算法的收敛性.当考虑评价网络的近似误差并满足假设条件时,迭代代价函数将收敛到最优代价函数的有界邻域.仿真例子验证了所提出方法的有效性.

关键词：自适应动态规划优化控制人工神经网络近似误差

Hypoxia-induced factor-1 alpha upregulates vascular endothelial growth factor C to promote lymphangiogenesis and angiogenesis in breast cancer patients

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The Journal of Biomedical Research 2013年第6期27卷 478-485页

作者： Xiaojian Ni Yingchun Zhao Jingjing Ma Tiansong Xia Xiaoan Liu qiang ding Xiaoming Zha Shui Wang Department of Breast Surgery The First Affiliated Hospital of Nanjing Medical University Department of Breast Surgery The Second People's Hospital Affiliated with Wannan Medical College State Key Laboratory of Reproductive Medicine Department of Breast Surgery Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University

Hypoxia-induced factor-1 alpha （HIF-1α） affects many effector molecules and regulates tumor lymphangio- genesis and angiogenesis during hypoxia. The aim of this study was to investigate the role of HIF-1α in the regu- lation of vascular endothelial growth factor C （VEGF-C） expression and its effect on lymphangiogenesis and an- giogenesis in breast cancer. Lymphatic vessel density （LVD）, microvessel density （MVD） and the expressions of HIF-1α and VEGF-C proteins were evaluated by immunohistochemistry in 75 breast cancer samples. There was a significant correlation between HIF-1α and VEGF-C （P = 0.014, r = 0.273, Spearman＇s coefficient of correlation）. HIF-1α and VEGF-C overexpression was significantly correlated with higher LVD （P = 0.003 and P = 0.017, re- spectively）, regional lymph nodal involvement （P = 0.002 and P = 0.004, respectively） and advanced tumor, node, metastasis （TNM） classification （P = 0.001 and P = 0.01, respectively）. Higher MVD was observed in the group expressing higher levels of HIF-1α and VEGF-C （P = 0.033 and P = 0.037, respectively）. Univariate analysis showed shorter survival time in patients expressing higher levels of HIF-1α and VEGF-C. HIF-1α was also found to be an independent prognostic factor of overall survival in multivariate analysis. The results suggest that HIF-1α may affect VEGF-C expression, thus acting as a crucial regulator of lymphangiogenesis and angiogenesis in breast cancer. This study highlights promising potential of HIF- 1α as a therapeutic target against tumor lymph node me- tastasis.

关键词： HIF-1α VEGF-C lymphangiogenesis angiogenesis breast cancer