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Evaluation of the excopula ejaculatory potentials of Bersama engleriana in spinal male rats

Asian Journal of Andrology 2009年第5期11卷 533-539,I0001页

作者： pierre watcho[喀麦隆] Miguel Carro-Juarez[喀麦隆] Animal Physiology and Phytopharmacology Laboratory University of Dschang Dschang P.O. BOX 67 Cameroon Laboratorio de Comportamiento Reproductivo Escuela de Medicina Veterinaria y Zootecnia Universidad Aut6noma deTlaxcala Tlaxcala C.P. 90000 M~xico

The aim of this study was to investigate the effects of Bersama engleriana and its potential mechanism on fictive ejaculation in spinal male rats. The electromyographic activities of the bulbospongiosus muscles were recorded in spinal cord transected and urethane-anesthetized rats treated intravenously with aqueous （100 mg kg^-1） and methanolic （100 mg kg^-1） extracts from the dried leaves of B. engleriana in the absence and presence of dopamine （0.1 μmol kg^-1） or oxytocin （0.5 UI kg^-1）. Mechanical stimulations of the urethra were also carried out 5 min after the sequential treatments. A single intravenous administration of aqueous （100 mg kg^-1） and methanolic （100 mg kg^-1） extracts of B. engleriana did not activate fictive ejaculation. The electromyography recorded after the application of the plant extract was similar to that obtained after intravenous saline injection （200 Gl min^-1） with no contraction of the bulbospongiosus muscles. Dopamine （0.1 μmol kg^-1） and oxytocin （0.5 UI kg^-1） induced rapid rhythmic contractions （P 〈 0.001） of the bulbospongiosus muscles accompanied by penile erection and sometimes with expulsion of the seminal plugs. Pretreatment of rats with the two plant extracts completely abolished the occurrence of ejaculation induced by dopamine （0.1 μmol kg^-1） and oxytocin （0.5 UI kg^-1）. Mechanical stimulation of the urethra carried out 5 min after the sequential treatments always induced penile movements and erections. The inhibitory effect of B. engleriana extracts on the expression offictive ejaculation in spinal male rat is mediated through dopaminergic and oxytocinergic pathways. This prolonged ejaculatory latency caused by B. engleriana could support its potential use in patients with rapid ejaculation.

关键词： Bersama engleriana dopamine ejaculation inhibition oxytocin spinal rat

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Aframomum melegueta prevents the ejaculatory complications of propylthiouracil-induced hypothyroidism in sexually experienced male rats: Evidence from intravaginal and fictive ejaculations

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Journal of Integrative Medicine 2019年第5期17卷 359-365页

作者： Francois Xavier Kemka Nguimatio Patrick Brice Deeh Defo Modeste Wankeu-Nya Esther Ngadjui Albert Kamanyi pierre Kamtchouing pierre watcho Animal Physiology and Phytopharmacology Laboratory University of DschangP.O. Box 67DschangCameroon Department of Animal Organisms Biology University of DoualaP.O. Box24157 DoualaCameroon Department of Animal Biology and Physiology University of Yaounde 1P.O. Box 812YaoundeCameroon

Objective: Hypothyroidism has been claimed to generate sexual dysfunctions such as ejaculatory disorders. Aframomum melegueta is an aphrodisiac plant with pro-ejaculatory properties. We investigated the protective effects of aqueous extract(AE) and methanolic extract(ME) of A. melegueta on the ejaculatory function of hypothyroid male ***: Forty sexually experienced male rats were partitioned into 8 groups(5 rats per group) and treated for 28 d as follows: Group 1, Control;Group 2, propylthiouracil(PTU, 10 mg/kg)+ distilled water(DW,10 m L/kg);Group 3, PTU + 5% Tween 80(10 m L/kg);Group 4, PTU + bromocriptine(6 mg/kg);Group 5,PTU + AE(20 mg/kg);Group 6, PTU + AE(100 mg/kg);Group 7, PTU + ME(20 mg/kg), and Group 8,PTU + ME(100 mg/kg). On days 0, 7, 14 and 28 of treatment, each male rat was paired with primed receptive female for measurement of ejaculatory latency time(ELT) and post-ejaculatory interval(PEI) for1.5 h. On day 29, each male rat was urethane-anesthetized and the spinal cord was ***, following urethral/penile stimulations and intravenous injection of dopamine, contractions of the bulbospongiosus muscles and the intraseminal pressure were registered. After these recordings,blood was collected through the catheterization of abdominal artery and plasma was used for thyroidstimulating hormone(TSH), prolactin and testosterone ***: PTU-induced hypothyroidism was characterized by a significant elevation(P < 0.001) of plasmatic TSH and prolactin levels, but a decline(P < 0.001) in plasmatic testosterone, compared to untreated group. ELT, PEI, contractions of the bulbospongiosus muscles and the intraseminal pressure were also altered by PTU treatment. On the contrary, A. melegueta extracts elevated testosterone(AE, 100 mg/kg,P < 0.01;ME, 100 mg/kg, P < 0.05) and decreased prolactin(AE, 100 mg/kg, P < 0.05;ME, 20 mg/kg,P < 0.05) levels, compared to corresponding controls. With regard to DW + PTU group, prolactin concentration wa

关键词： Propylthiouracil Hypothyroidism Ejaculation Bromocriptine Aframomum melegueta

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Effects of Ficus asperifolia on normal rat estrus cyclicity

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Asian Pacific Journal of Tropical Biomedicine 2013年第1期3卷 53-57页

作者： Esther Ngadjui pierre watcho Telesphore Benoit Nguelefack Albert Kamanyi Department of Animal Biology Faculty of ScienceUniversity of Dschang

Objective:To evaluate Ficus asperifolia(Moraceae)(***)effecting on regular estrus cycle of Wistar ***:Air-dried fruits of *** were extracted using *** to the test,vaginal smear was monitored daily for a 3-week period to select females with normal(regular)estrous *** with regular estrus cycle weighing between 150-170 g were randomized into three sets of 15 animals *** set was then divided into three groups:Group 1(control)was orally administered with distilled water(10 mL/kg body weight)once a day for 1 week starting from the proestrus *** 2 and 3 were respectively treated with 100and 500 mg/kg body weight of the plant aqueous *** two other sets of 15 animals each were similarly treated as the first set for 3 weeks and 6 weeks *** cycle pattern was monitored before and during plant extract application whereas lipid profile,ovary,uterus and liver growth indices were determined at the end of each ***:*** did not disrupt(0%)the order of appearance of normal estrus cycle stages,namely,proestrus,estrus,metestrus and ***-term treatment(1 week duration)exhibited high frequency of appearance of proestrus and estrus stages while mid-(3 weeks)and long-term(6 weeks)treatments revealed constancy in the frequency of all stages irrespective to animal *** plasma and organ lipid profile,as well as ovary,uterus and liver growth remained unchanged when compared to distilled water-treated *** long-term administration of plant extract(6 weeks),no adverse effect was ***:Our data partially support the use of *** in common medicine.

关键词： Ficus asperifolia Estrus cycle Rats.

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Cyclophosphamide-induced reproductive toxicity: Beneficial effects of Helichrysum odoratissimum(Asteraceae) in male Wistar rats

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Journal of Integrative Medicine 2019年第5期17卷 366-373页

作者： pierre watcho Ismaelle Rosine Mpeck Patrick Brice Deeh Defo Modeste Wankeu-Nya Esther Ngadjui Georges Romeo Bonsou Fozin pierre Kamtchouing Albert Kamanyi Animal Physiology and Phytopharmacology Laboratory University of DschangP.O. BOX 67DschangCameroon Department of Animal Organisms Biology University of DoualaP.O. BOX 24157DoualaCameroon Department of Animal Biology and Physiology University of YaoundeP.O. BOX 812YaoundeCameroon

Objective: Cyclophosphamide(CP) is commonly used to treat some cancers, but its clinical efficacy is also linked with testicular toxicity. We investigated the effects of aqueous extract(AE) and methanolic extract(ME) of Helichrysum odoratissimum for reducing CP-induced reproductive toxicity in male ***: In addition to a normal control(group 1), drugs or vehicles were administered orally to seven groups(n = 5) of rats that had already received 4-weeks of pre-treatment with CP(5 mg/[kgád], per oral administration);group 2 received CP + distilled water(10 m L/[kgád]);group 3 received CP + 5% tween 80(10 m L/[kgád]);group 4 received CP + clomiphene citrate(0.25 mg/[kgád]);groups 5 and 6 received CP + AE(50 and 100 mg/[kgád]) and groups 7 and 8 received CP + ME(50 and 100 mg/[kgád]). Animals were sacrificed on day 15, and body and sexual organ weights, sperm characteristics, testosterone level and testicular histology were ***: The CP-treated group showed a significant reduction(P < 0.001) in the body and seminal vesicle weights, testosterone level, sperm count, sperm motility and sperm viability, but elevated(P < 0.001)sperm morphological abnormalities and testicular structure alterations, compared to the control ***, these detrimental effects of CP were reversed by treatment with H. odoratissimum *** instance, both extracts and all doses of H. odoratissimum significantly increased the sperm count(P < 0.001), sperm motility(AE, 50 mg/kg, P < 0.05;ME, 50 and 100 mg/kg, P < 0.05) and sperm viability(AE, 50 mg/kg, P < 0.001;ME, 50 and 100 mg/kg, P < 0.001) compared to the CP group. H. odoratissimum also improved plasmatic and intratesticular testosterone levels and prevented histological alterations of the ***: H. odoratissimum might be considered as an alternative drug to alleviate/prevent reproductive damage in cancer patients receiving CP chemotherapy.

关键词： Helichrysum odoratissimum Cyclophosphamide Reproductive toxicity Rat

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Androgenic effect of Mondia whitei roots in male rats

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Asian Journal of Andrology 2004年第3期6卷 269-272页

作者： pierre watcho pierre Kamtchouing Selestin D.Sokeng Paul F.Moundipa Justine Tantchou Jean L.Essame Noussithe Koueta Animal Physiology and Phytopharmacology Laboratory Faculty of ScienceUniversity of DschangDschangCameroon Animal Physiology Laboratory University of Yaounde IYaoundeCameroon Department of Biochemistry Faculty of SciencesUniversity of Yaounde IYaoundeCameroon 不详 University Hospital Centre YaoundeCameroon

Aim: To determine the effect of the aqueous extract of Mondia whitei (Periplocaceae) roots on testosterone production and fertility of male rats. Methods: Adult male Wistar rats were used. In the acute study, 20 rats were randomly divided into 5 groups of 4 animals each. Four treated groups were administered orally a single dose of Mondia whitei (400 mg/kg) and the controls received a similar amount of distilled water. One group of animals were sacrificed by cervical dislocation 1,2,4 and 6 h after treatment, respectively. The controls were sacrificed at 6 h. Testicular testosterone was determined by radioimmunoassay. In the chronic study, 28 rats were divided at random into 4 groups of 7 animals each: Groups 1, 2 and 3 were given orally the plant extract (400 mg·kg-1·day-1) for 2, 4 and 8 days, respectively. The animals of Groups 1 and 2 were sacrificed 24 hours after the last dosing. The controls (Group 4) received the same amount of distilled water for 8 days. The fertility was assessed only in Groups 3 and 4 and after that, the animals were sacrificed and the epididymal sperm density, the serum testosterone and the testicular testosterone and 17β-estradiol were assayed. The serum, testicular and epidydimal protein contents were also determined. Results: In the acute treatment groups, the serum and testicular concentrations of testosterone remained unchanged at all the time points. Chronic treatment for 8 days induced a significant increase in the testicular weight, the serum and testicular testosterone, the testicular protein content and the sperm density (P<0.05-0.01), but did not affect the accessory gland weights, the serum protein contents, the testicular concentration of 17β-estradiol and the fertility compared to the controls. Conclusion: Mondia whitei root extract possesses an androgenic property.

关键词： Mondia whitei testosterone sperm density fertility

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The methanolic extract ofGuibourtia tessmannii(Caesalpiniaceae)improves sexual parameters in high fat diet-induced obese sexually sluggish rats

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Asian pacific Journal of Reproduction 2017年第5期6卷 202-211页

作者： Patrick Brice Deeh Defo Modeste Wankeu-Nya Esther Ngadjui Georges Romeo Bonsou Fozin Francois Xavier Kemka Albert Kamanyi pierre Kamtchouing pierre watcho Animal Physiology and Phytopharmacology Laboratory University of DschangBox 67 DschangCameroon Department of Animal Organisms Biology University of Douala-Cameroon Department of Animal Biology and Physiology University of Yaoundé1YaoundéCameroon

Objective:To evaluate the effects of the methanolic extract ofGuibourtia tessmannii (G. tesmannii) on sperm parameters, lipid profile and testosterone level in obese ***: A total of 193 male Wistar rats were fed either with palm oil diet (n=185) or standard diet (n=8) for 16 wk. At the end of this feeding period, 90 obese rats were selected and randomly divided into 18 groups of five rats each and treated with distilled water (10 mL/kg), vitamin E (75 mg/kg), clomiphene citrate (2 mg/kg) or methanolic extract ofG. tessmannii (55, 110 or 220 mg/kg) for 7, 21 or 56 d. At the end of each treatment period, sperm parameters, lipid profile and testosterone level were evaluated. Data were analyzed using ANOVA for repeated measures followed by post-hoc Tukey HSD (P<0.05) for multiple ***: Feeding of rats for 16 wk with palm oil diet significantly damaged sperm parameters. The methanolic extract ofG. tesmannii improved sperm viability, motility and normality after 21 or 56 d of treatment. The sperm normality increased significantly in rats treated with the methanolic extract ofG. tesmannii for 7 (110 mg/kg,P<0.01) and 56 d (110 and 220 mg/kg,P<0.05) compared to control group. Triglycerids, total cholesterol, low and very low density lipoproteins cholesterol levels were lower in rats treated with the plant extract for 56 d.G. tesmannii also significantly increased the high density lipoprotein cholesterol and testosterone levels in the plasma after 56 d of ***: The methanolic extract ofG. tesmannii can improve sperm parameters, lipid profile and testosterone level in obese rats. These findings may justify the folkloric use ofG. tesmannii as a reproductive performance enhancer.

关键词： Guibourtia tessmannii Palm oil diet Sperm parameters Lipid profile Testosterone

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Treatment of peripheral diabetic neuropathy in Zucker diabetic fatty (ZDF) rats with cariporide

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Journal of Diabetes Mellitus 2014年第1期4卷 59-66页

作者： Sergey Lupachyk Hanna Shevalye pierre watcho Alexander Obrosov Irina G. Obrosova Mark A. Yorek Department of Veterans Affairs Iowa City Health Care System and Department of Internal Medicine University of Iowa Iowa City USA Pennington Biomedical Research Center Louisiana State University System Baton Rouge USA

To determine the effect of Na+/H+-exchanger-1 on diabetic neuropathy in Type 2 diabetes Zucker diabetic fatty (ZDF) rats and lean controls were treated with cariporide (10 or 20 mg/kg/d), a Na+/H+-exchanger-1 inhibitor, for 4 weeks at 15 weeks of age. Neuropathy endpoints included motor (MNCV) and sensory (SNCV) nerve conduction velocities, thermal nociception, tactile allodynia and intraepidermal nerve fiber density. Advanced glycation endproduct and markers of oxidative stress including nitrated protein levels in sciatic nerve and dorsal root ganglion were also evaluated. Expression of Na+/H+-exchanger-1 in dorsal root ganglion neurons was increased in ZDF rats. At 15 weeks of age ZDF rats displayed hyperglycemia, MNCV and SNCV deficits, thermal hypoalgesia and tactile allodynia. At 20 but not 10 mg/kg/d, cariporide significantly improved MNCV and SNCV deficits, thermal hypoalgesia and tactile allodynia. Cariporide treatment was also associated with reduction of diabetes-induced accumulation of advanced glycation end-product (AGE), oxidative stress and nitrated proteins in sciatic nerve and dorsal root ganglion neurons. In conclusion, these findings support an important role for Na+/H+-exchanger-1 in peripheral diabetic neuropathy, and provide rationale for development of Na+/H+-exchanger-1 inhibitors for treatment of diabetic neural complications.

关键词： Diabetic Neuropathy Sodium/Hydrogen Exchanges Oxidative Stress

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Effects of the Mixture of Erigeron floribundus(Asteraceae) and Tragia benthamii(Euphorbiaceae) on the Growth and Architecture of Estrogen-Sensitive Sexual Organs

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Pharmacology & Pharmacy 2021年第12期12卷 269-282页

作者： Gladis Komguep Djuidje Esther Ngadjui Aimé Césaire Momo Tetsatsi Georges Romeo Bonsou Fozin pierre watcho Research Unit of Animal Physiology and Phytopharmacology Faculty of Science University of Dschang. Dschang Cameroon

The mixture of Erigeron floribundus and Tragia benthamii (AEMEFTB) is traditionally used against pelvic pain, dysmenorrhea and female sexual dysfunctions. In a recent study, we showed that the aqueous extract of the mixture of AEMEFTB suppresses the endometrium growth in rats with experimental endometriosis. The present study was aimed at investigating the effects of AEMEFTB on estrogen’s sensitive sexual organs growth and architecture. Immature gonado-intact female rats were randomly distributed into 7 groups of 5 animals each and daily treated during one week with either distilled water (10 ml/kg), refined palm oil (1 ml/kg) or 17β-estradiol (1 μg/day). Plant extract groups received aqueous extract of AEMEFTB at 130 or 260

关键词： Estrogen Erigeron floribundus Rat Sexual Organs Tragia benthamii

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Oxidized palm oil impairs reproductive functions and architectures in female rats

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Asian pacific Journal of Reproduction 2023年第4期12卷 182-193页

作者： Wankeu-Nya Modeste Kengne Inès T Ateba Benjamin S Bend Fortune E Djeumeni Ornela N HathoDominique T Ngadjui E Moundipa Paul F Massoma DieudonnéL Dongmo Alain B watcho pierre Department of Animal Organisms Biology Faculty of ScienceUniversity of DoualaDoualaCameroon Department of Animal Production Faculty of Agronomy and Agricultural SciencesUniversity of DschangDschangCameroon Department of Biochemistry Faculty of ScienceUniversity of YaoundéIYaoundéCameroon Department of Animal Biology Faculty of ScienceUniversity of DschangDschangCameroon

Objective:To evaluate the effects of three oxidized palm oil diets(OPD)on female rat reproductive ***:Forty-four female Wistar rats presenting five consecutive and regular estrous cycles were divided into 4 *** rats were fed with:a standard diet,70%of standard diet+30%oxidized palm oil diet(OPD1),OPD1+5 g of boiled yolk egg(OPD2)and OPD1+10%sucrose(OPD3)for 125 days,*** the feeding period,morphometric,estrous cycle,sexual behavior,gestation,biochemical and histomorphometric parameters were ***:All OPDs significantly increased abdominal circumference,body mass index and Lee index coupled to an irregularity and lengthening of the estrous *** significantly decreased appetite and consumption behaviours,quantic pregnancy index,fertility rate,implantation sites and index,serum progesterone and high-density lipoprotein levels,increased pre-implantation losses,anti-implantation activities,serum estradiol,triglycerides,total and low-density lipoprotein-cholesterol levels,and impaired brain and ovaries oxidative *** examinations revealed increases in the number of atresic and primary follicles and decreases in secondary,tertiary,Degraaf,total and corpus luteum follicles in ovaries coupled to a neurodegeneration of hypothalamic anteroventral periventricular neurons in the OPD groups compared to the standard diet ***:The three OPDs induce obesity and impair the female reproductive function,especially OPD2 and *** findings contribute to a better understanding of the adverse effects of palm oil bleaching on the reproductive function in female rats,which could be useful in the management of women with obesity-related sexual dysfunction.

关键词： Bleached palm oil Reproductive function Anteroventral periventricular nucleus Ovary Estrogen Progesterone Female rat

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Long Term Administration of Lannea acida Rich. (Anacardiaceae) Reverses the Imidacloprid-Induced Fertility Impairments in Adult Male Rat through Androgenic and Antioxidant Properties

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Journal of Biosciences and Medicines 2024年第3期12卷 62-76页

作者： Aimé Césaire Momo Tetsatsi Gladis Komguep Djuidje Prechmy Carole Ngueyong Nsamou Karl Gombeu Zeugang Yannick Tchatat Petnga Désiré Alumeti Munyali Esther Ngadjui Pepin Alango Nkeng-Effouet pierre watcho Faculty of Health Sciences The University of Bamenda Bamenda Cameroon Animal Physiology and Phytopharmacology Laboratory Faculty of Science University of Dschang Dschang Cameroon Laboratory of Immunology and Molecular Biology The Evangelical University of Cameroon Bandjoun Cameroon School of Medicine and Community Health Evangelical University in Africa Bukavu DR Congo Alango Foundation Center for African Phytomedicine Dschang Cameroon

Aim: The harmful effects of pesticides have been largely documented in recent times. But effective therapeutic solutions to pesticide related male infertility are yet to be established. This study investigated the curative effects of Lannea acida on imidacloprid (IMI)-induced hypofertility in male Wistar rats. Methods: Rats of 150 – 200 g were administered IMI (22.5 mg/kg) for two weeks and partitioned into control (distilled water, vitamin E, clomiphene citrate) or test (aqueous (340 mg/kg), methanol (170 mg/kg) extract) groups for eight weeks treatment. Animals were sacrificed at the end of the treatment and samples were collected for sperm, antioxidant and hormonal analysis. Fertility tests were performed from treatment day 47 for fertility indices estimation. Results were expressed as mean ± SEM and one way ANOVA was applied using STATISTICA Software. Results: Exposition to IMI resulted in a significant decrease in sperm count, motility, viability and normality, testosterone and LH, coupled to an increase in oxidative stress markers. Moreover, IMI impaired male fertility evidenced by a significant drop in fertility index and litter size. Similar to clomiphene citrate and vitamin E, plant extracts significantly improved the sperm parameters, sexual hormones and decreased the oxidative stress markers. More importantly, the fertility index and litter size were restored, especially with the aqueous extract. Conclusion: Present results indicate that L. acida possesses curative potentials against IMI-induced hypofertility through its androgenic and antioxidant properties. However, the effects the extract on spermatozoa DNA structure and the fertility of offsprings from exposed parents are yet to be studied to conclude on total recovery from IMI toxicity.

关键词： Imidacloprid Lannea acida Hypofertility Male Rat

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