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茶多酚（TP）饲料添加剂对尼罗罗非鱼生长、体成分、血液生化、抗氧化和免疫能力的影响

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茶多酚（TP）饲料添加剂对尼罗罗非鱼生长、体成分、血液生化、抗...

作者： CHRISTOPHER peterson DANIEL 南京农业大学

学位级别：硕士

茶多酚(TP)是水产养殖领域中最常用的中草药之一。本研究旨在研究该草药对尼罗罗非鱼的生长,饲料利用和转化,鱼体成分,抗氧化活性和免疫反应的影响。在实验开始之前,配制了六种饲粮,分别添加0.00 mg/kg,100 mg/kg,200 mg/kg,300 mg/kg,4... 详细信息

茶多酚(TP)是水产养殖领域中最常用的中草药之一。本研究旨在研究该草药对尼罗罗非鱼的生长,饲料利用和转化,鱼体成分,抗氧化活性和免疫反应的影响。在实验开始之前,配制了六种饲粮,分别添加0.00 mg/kg,100 mg/kg,200 mg/kg,300 mg/kg,400 mg/kg和500 mg/kg茶多酚。在开始投喂实验前,先将600条鱼放在300L的水箱中进行驯化,为期两周。在此期间,投喂商品饲料。驯化期结束后,将15尾平均体重(13.46±1.25 g)相近的健康幼鱼随机分为4个重复,在6个(150L)周转箱中,按其体重的5%,每天人工喂食3次,共8周。在为期8周的饲喂试验后,饲喂100 mg/kg茶多酚的鱼显示出较高的最终体重(FBw),增重率(wGR)和特定生长率(SGR),均无显著性(P>0.05)。与以0.00 mg/kg喂食的对照组不同,以500 mg/kg喂食的组显示出最低的最终体重(FBw)。100 mg/kg饲喂组的饵料系数(FCR)较低,但与对照组无显著性差异(P>0.05)。对照组的0.00 mg/kg脂肪含量略高,但与其他治疗组无显著差异(P>0.05)。饲喂100 mg/kg饲料组的水分含量较高。水分含量与对照组相比有显著性差异(PwGR)和特定生长率(SGR),降低肝脏与身体的比例以及内脏与身体的比例,在某种程度上提高了鱼体的非特异性免疫能力,但在高剂量(大于300 mg/kg)时抑制了生长。日粮种添加茶多酚(TP)对尼罗罗非鱼血液生化指标,抗氧化反应和免疫的影响这项研究旨在评估日粮中添加茶多酚对尼罗罗非鱼(GIFT)血液生化指标、抗氧化反应和机体免疫的影响。每个桶采集4条鱼的血样,4℃4000 g快速离心10 min后分离血浆。在实验室进行了血液生化分析和抗氧化功能的研究。为了研究形态变化,从前到后依次扫描样品,然后使用Semaphore软件拍摄受影响区域的数字图像。饲喂不同量的茶多酚对罗非鱼的血液生理和生化有一定的影响。其中,茶多酚的添加显著降低了血液中甘油三酯的含量(TG)(P0.05)。在鱼饲料中添加高剂量的茶多酚(400和500 mg/kg)后,罗非鱼肝脏中的超氧化物歧化酶(SOD),过氧化氢酶(CAT)和总抗氧化能力(T-AOC)也有显著提高的趋势(P<0.05)。低剂量组(100 mg/kg)肝脏丙二醛(MDA)含量显著高于对照组(P<0.05)。每桶收集10条实验鱼,为了引起急性肝损伤,采用四氯化碳(CCl4),每100 g鱼体重腹腔注射0.5 ml溶液(50%)。注射后6h,12h,1d,2d,3d,4d,5d,6d和7d观察鱼的死亡情况。随着饲粮中添加茶多酚量的增加,四氯化碳溶液注射剂诱导的累计死亡率逐渐增加。对照组0.00 mg/kg和100mg/kg组的累积死亡率明显低于500 mg/kg组(P<0.05)。采样期间,在高剂量组(400 mg/kg和500 mg/kg)的罗非鱼肝脏中观察到典型的胆汁浸润,胆囊和脾脏肿大,颜色加深,消化道易碎且易破裂,水肿结节等现象。对罗非鱼肠和肝细胞凋亡的组织进行染色,组织中受损的凋亡细胞的细胞核被染成黄棕色,正常组织的细胞核被染成蓝色。染色结果表明,随着茶多酚(TP)剂量的增加,罗非鱼肠道和肝脏中凋亡细胞的数量减少。肠道组织切片的结果显示,茶多酚对罗非鱼消化道的生理功能有明显的影响。茶多酚含量的增加会导致消化道水肿,炎症性损伤和细胞纤维化。

关键词：罗非鱼生长性能抗氧化反应免疫能力

Large-Scale Genome Programs at the National Human Genome Research Institute NIH

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Large-Scale Genome Programs at the National Human Genome Res...

第二届传染病诊治高峰论坛暨2009年浙江省感染病、肝病学术年会

作者： Jane L.peterson Associate Director Division of Extramural Research Program Director Large-scale Sequencing NHGRI/NIH

The National Human Genome Research Institute(NHGRI) is one of the Institutes of the National Institutes of Health(NIH) in the *** supports development of genomic technologies and large genomic resources for community ... 详细信息

关键词： Large-Scale Genome Programs at the National Human Genome Research Institute NIH

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由三叶形纽结得到的三维流形的不变量

科学通报 1996年第21期41卷 1924-1930页

作者：李邦河李起升 Chariya peterson 中国科学院系统科学研究所河南大学数学系 Department.of Mathematics Northwestern UniversityEvanstonIL 60206 USA

关于witten不变量,已有不少研究,可参见文献[1～9].Freed和Gompf及Neil曾对若干三维流形witten不变量τ_r(M)用计算机进行了近似的数值计算.本文给出所有由三叶结作整系数换球术得出的三维流形的不变量(?)_p(M,A)的计算公式(定理1),并... 详细信息

关于witten不变量,已有不少研究,可参见文献[1～9].Freed和Gompf及Neil曾对若干三维流形witten不变量τ_r(M)用计算机进行了近似的数值计算.本文给出所有由三叶结作整系数换球术得出的三维流形的不变量(?)_p(M,A)的计算公式(定理1),并通过计算机对p≤13进行符号计算,得到了几个有意思的结论.文中的记号是标准的,可参见文献[3,6,9],不另说明.若记L_n和R_n分别是由左手和右手标架为n的三叶结作换球术得到的三维流形,则L_(-n)和R_n是反向同胚的,于是(?)p(R_n,A)(?)(?)p(L_(-n),A),因此只需计算(?)p(Ln,A).记是m分支的标架全为零的链环,则有引理1〈e_(i_1),…,e_(i_m)〉k_m=e_(i_1)(λ_(i_2))…e_(i_(m-1))(λ_(i_m))〈e_(i_m)〉其中λ_j=-A^(2j+2)-A^(-2j-2).

关键词：纽结三维流形不变量流形三叶形纽结

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大豆中与人体健康有关的生理活性成分

食品工业科技 1998年第4期19卷 78-79页

作者：张慧敏 D.peterson 北京农学院食品科学系西澳农业研究院

大豆中与人体健康有关的生理活性成分张慧敏（北京农学院食品科学系，北京１０２２０６）Ｄ．Ｐｅｔｅｒｓｏｎ（西澳农业研究院，澳大利亚６１５１）１前言大豆（Ｇｌｙｃｉｎｅｍａｘ（Ｌ．）Ｍｅｒｒｉｌ）食品传统上广泛用于东方膳... 详细信息

大豆中与人体健康有关的生理活性成分张慧敏（北京农学院食品科学系，北京１０２２０６）Ｄ．Ｐｅｔｅｒｓｏｎ（西澳农业研究院，澳大利亚６１５１）１前言大豆（Ｇｌｙｃｉｎｅｍａｘ（Ｌ．）Ｍｅｒｒｉｌ）食品传统上广泛用于东方膳食中。例如，中国各种豆腐制品，印度...

关键词：大豆生理活性成分食品

Nucleotide Sequence and Characterization of Bovine Metallothionein Gene

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Chinese Science Bulletin 1993年第5期38卷 419-423页

作者：杨靖沈孝宙杨卫民 M.G.peterson J.F.B.Mercer Savio L.C.woo Institute of Developmental Biology Academia Sinica Beijing 100080 PRC. Laboratory of Reproductive Biology Institute of Zoology Academia Sinica Beijing 100080 PRC Birth Defects Research Institute Poyal Children's Hospital Parkville Australia Department of Cell Biology Baylor College of Medicine Houston TX 77030 USA

1 Introduction Metallothioneins (MTs) are a family of low-molecular weight heavy metal binding proteins, unique in their high cystein content. MTs are ubiquitously distributed in the eukaryote from yeast to human. It is an experimental model emphasized during researching gene expression and regulation of animal cells. In order to provide inducible regulatory elements for transgenosis livestock, we cloned bovine MT genes. At least four members of bovine MT family have been discovered. In this note, one gene (bMTc) of

关键词： bovine metallothionein gene DNA sequence gene structure.

CLONING AND ORGANIZATION OF BOVINE METALLOTHIONEIN GENES

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Chinese Science Bulletin 1989年第11期34卷 953-956页

作者：沈孝宙杨卫民彭红 M.G.peterson J.F.B.MERCER SAVIO L.C.wOO Laboratories of Reproductive Biology and Department of Endocrinology Institute of Zoology Academia Sinica Beijing Birth Defects Research Institute Royal Children's Hospital Parkville Australia Department of Cell Biology Baylor College of Medicine Texas Medical Center Houston TX 77030 USA

I. INTRODUCTIONMetallothioneins(MT) are ubiquitously distributed in animal and plant kingdoms. They are cysteine-rich proteins that specifically bind heavy metal ions, such as cadmium, zinc, mercury and copper. The high expression of the MT gene could be induced by heavy metals in vitro and in vivo. The first transgenic mice obtained by Palmiter et al. in 1982 were introduced with an exogenous growth hormnoe gene

关键词： bovine metallothionein gene gene organization genomic cloning.

RESTORATION OF SUPPRESSED IMMUNITY IN BURNED MICE wITH CIMETIDINE

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中华医学杂志(英文版) 1985年第5期98卷 384-387页

作者： R Zapata-Sirvent JF Hansbrough V peterson H Claman Burn and Trauma Research Laboratory Department of Surgery University of Colorado Health Sciences Center Denver CO China

Thermal injury has been shown to result in suppression of many aspects of immunity. we studied the effect of cimetidine on suppressed cell mediated immunity in burned mice, utilizing a method of quantitating sensitivity to the contact antigen, 2,4-dinitrofluorobenzene. Daily administration of cimetidine resulted in maintenance of normal cell mediated immunity in these otherwise immunosuppressed animals. This effect may be secondary to the antagonism of cimetidine with histamine H2receptors on suppressor cells. Immunorestoration with cimetidine may improve resistance of the burned host to infectious complications.

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Outcomes of Medicare beneficiaries hospitalised with transient ischaemic attack and stratification using the ABCD^(2) score

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Stroke & Vascular Neurology 2021年第2期6卷 314-318页

作者： Shreyansh Shah Li Liang Durgesh Bhandary Saga Johansson Eric E Smith Deepak L Bhatt Gregg C Fonarow Naeem D Khan Eric peterson Janet Prvu Bettger Department of Neurology Duke University School of MedicineDurhamNorth CarolinaUSA Duke Clinical Research Institute DurhamNorth CarolinaUSA AstraZeneca UK Ltd CambridgeUK Hotchkiss Brain Institute and Department of Clinical Neurosciences University of CalgaryCalgaryAlbertaCanada Division of Cardiology Brigham and Women's HospitalBostonMassachusettsUSA Division of Cardiology Ronald Reagan UCLA Medical CenterLos AngelesCaliforniaUSA

Background Long-term outcomes for Medicare beneficiaries hospitalised with transient ischaemic attack(TIA)and role of ABCD^(2) score in identifying high-risk individuals are not *** we identified 40825 Medicare beneficiaries hospitalised from 2011 to 2014 for a TIA to a Get with The Guidelines(GwTG)-Stroke hospital and classified them using ABCD^(2)s of mortality and rehospitalisation(all-cause,ischaemic stroke,haemorrhagic stroke,myocardial infarction,and gastrointestinal and intracranial haemorrhage)for high-risk versus low-risk groups adjusted for patient and hospital *** Of the 40825 patients,35118(86%)were high risk(ABCD^(2)≥4)and 5707(14%)were low risk(ABCD^(2)=0-3).Overall rate of mortality during 1-year follow-up after hospital discharge for the index TIA was 11.7%,44.3% were rehospitalised for any reason and 3.6%were readmitted due to *** with ABCD^(2) score≥4 had higher mortality at 1 year than not(adjusted HR 1.18,95%CI 1.07 to 1.30).Adjusted risks for ischaemic stroke,all-cause readmission and mortality/all-cause readmission at 1 year were also significantly higher for patients with ABCD^(2) score≥4 vs *** contrast,haemorrhagic stroke,myocardial infarction,gastrointestinal bleeding and intracranial haemorrhage risk were not significantly different by ABCD^(2) *** This study validates the use of ABCD^(2) score for long-term risk assessment after TIA in patients aged 65 years and *** efforts for community-based follow-up care after TIA are needed for ongoing prevention in Medicare beneficiaries who were hospitalised for TIA.

关键词： admitted score prevention

Evolving governance during health care reform in the United States and China

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中国研究型医院 2014年第1期1卷 78-89页

作者： Donghai wei Louis Rubino Robert peterson Guangzhou Medical University Guangdong Provincial Health Economics Association California State University

Governance of public hospitals has become an important consideration in China. Research is being conducted, consultants are being engaged, and new models are being tested to determine how a change in governance can positively affect health care reform. The United States is looked to as an example for hospital governance. Old principles and new trends for boards of directors of hospitals are discussed. Critical thought is given as to the governance principles that can be applied in China which will augment the changes taking place to make hospitals more autonomous under health care reform. Recommendations are made by the authors as to which of these principles and new methods should be considered to be adopted for China's public hospitals.

关键词： Governance Board of Directors Fiduciary Responsibility Public Hospitals Strategic Direction